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Gottfried O. H. Naumann - One of the best experts on this subject based on the ideXlab platform.
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Reduction in number of Corpora amylacea with advancing histological changes of glaucoma
Graefe's Archive for Clinical and Experimental Ophthalmology, 1993Co-Authors: Toshiaki Kubota, Gottfried O. H. NaumannAbstract:Retina and optic nerve of eyes enucleated due to an iris ring melanoma or a malignant melanoma of the ciliary body were studied to investigate the correlation between Corpora amylacea count and glaucoma stages. Ten eyes from ten patients [age: 62.3±13.0 (mean±SD); range 43-84 years] were used for the present study. Eight eyes had shown elevated intraocular pressure readings preoperatively. The Corpora amylacea count was correlated with histomorphometric changes of the intra- and parapapillary region. The Corpora amylacea count of the retinal ganglion cells decreased significantly with increased histological ratio of cup to disc, decreased retinal nerve fiber layer thickness, and increased optic cup depth. There was no significant correlation between the Corpora amylacea count of the bipolar cells and each histomorphometric datum. These results suggest that the Corpora amylacea of the retinal ganglion cells decrease in number with advancing histological changes of secondary glaucoma.
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Corpora amylacea in glaucomatous and non-glaucomatous optic nerve and retina
Graefe's Archive for Clinical and Experimental Ophthalmology, 1993Co-Authors: Toshiaki Kubota, Leonard M. Holbach, Gottfried O. H. NaumannAbstract:We studied the occurrence, location, and size of Corpora amylacea (CA) in periodic acid-Schiff (PAS)-stained histological sections of the retina and optic nerve. The glaucoma group included 48 blind eyes obtained from 48 patients (mean age: 64±13.9 years; range 26–83 years) with advanced secondary angle-closure glaucoma. The non-glaucomatous group consisted of 45 non-glaucomatous eyes from 45 patients (mean age: 62.1±12.2 years, range 34–78 years) suffering from malignant melanoma of the choroid, and six autopsy eyes obtained from young individuals (age range 2.5–29 years). The mean diameter of CA at the level of the retinal ganglion cells (retrolaminar: 10.92±5.15 µm, intralaminar: 10.97±5.04 µm, prelaminar: 9.17±4.53 µm, nerve fiber layer: 8.56±4.27 µm) was significantly larger ( P
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Corpora amylacea in glaucomatous and non glaucomatous optic nerve and retina
Graefes Archive for Clinical and Experimental Ophthalmology, 1993Co-Authors: Toshiaki Kubota, Leonard M. Holbach, Gottfried O. H. NaumannAbstract:We studied the occurrence, location, and size of Corpora amylacea (CA) in periodic acid-Schiff (PAS)-stained histological sections of the retina and optic nerve. The glaucoma group included 48 blind eyes obtained from 48 patients (mean age: 64±13.9 years; range 26–83 years) with advanced secondary angle-closure glaucoma. The non-glaucomatous group consisted of 45 non-glaucomatous eyes from 45 patients (mean age: 62.1±12.2 years, range 34–78 years) suffering from malignant melanoma of the choroid, and six autopsy eyes obtained from young individuals (age range 2.5–29 years). The mean diameter of CA at the level of the retinal ganglion cells (retrolaminar: 10.92±5.15 µm, intralaminar: 10.97±5.04 µm, prelaminar: 9.17±4.53 µm, nerve fiber layer: 8.56±4.27 µm) was significantly larger (P<0.0001; Wilcoxon-Mann-Whitney test) than their size at the level of the bipolar cells (inner plexiform layer: 3.79±1.30 µm). The count of CA in sections from non-glaucomatous subjects aged 2.5 to 78 years (45 eyes with malignant melanoma and 6 autopsy eyes) increased significantly (P<0.01) with advancing age. CA occurred significantly more often (P<0.0001) in eyes with melanoma (45.7±29.4 per section) than in eyes with glaucoma (4.7±6.9 per section). These results suggest that CA represent intraneuronal aging products that are diminished in eyes with end-stage glaucoma due to neuronal loss.
H M Schipper - One of the best experts on this subject based on the ideXlab platform.
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Role of heme oxygenase-1 in the biogenesis of Corpora amylacea
Biogerontology, 2002Co-Authors: D.j. Sahlas, A. Liberman, H M SchipperAbstract:Corpora amylacea (CA) are glycoproteinaceous inclusions that accumulate in the human brain during normal aging and to a greater extent in Alzheimer's disease. We previously demonstrated that, in cultured rat astroglia, cysteamine (CSH) upregulates heme oxygenase-1(HO-1) and promotes the transformation of normal mitochondria into CA-like inclusions. In the current study, primary cultures of neonatal rat astroglia were exposed to880 µM CSH for three months in the presence or absence of dexamethasone, a suppressor ofHO-1 gene transcription. Cells were double-labeled with periodic acid-Schiffreagent (PAS) and antisera against ubiquitin,HO-1, or a mitochondrial epitope. CA were quantified and their immunostaining characteristics analyzed using confocalmicroscopy.HO-1 immunofluore scence was more abundant in cultures exposed to CSH alone relative to untreated control cultures and cultures exposed to both CSH and dexamethasone. Mature CA appeared as large (5–50 µM), spherical orpolygonal, intensely PAS-positive inclusions within glial cytoplasm or deposited extracellularly. The inclusions manifested intense rim and, less commonly, homogeneous or stippled patterns of immunoreactivity for ubiquitin, HO-1, and the mitochondrial marker. Monolayers exposed to CSH exhibited 660% more CA relative to untreated controls ( P < 0.05). Numbers of CA in cultures exposed to CSH were diminished by co-administration of 50 µg/ml dexamethasone ( P < 0.05 relative to CSH alone)or 100 µg/ml dexamethasone ( P < 0.05relative to CSH alone). Numbers of CA incultures co-treated with CSH and 50 µg/mldexamethasone or 100 µg/ml dexamethasone were not significantly different from untreated control values. Up-regulation of HO-1 may contribute to the formation of CA in aging astroglia.
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experimental induction of Corpora amylacea like inclusions in rat astroglia
Neuropathology and Applied Neurobiology, 1995Co-Authors: S Cisse, H M SchipperAbstract:Corpora amylacea (CA) are glycoproteinaceous inclusions that accumulate in the human central nervous system during normal ageing, and to an even greater extent in Alzheimer's disease and other neurodegenerative disorders. They are particularly prominent in subpial and subependymal regions, and are most commonly located within astrocytes and their processes. We previously demonstrated that human CA share many tinctorial and histochemical properties in common with Gomori-positive cytoplasmic granules which accumulate in periventricular astrocytes of the ageing vertebrate brain and in rat astroglial cultures exposed to the sulphydryl agent, cysteamine (CSH). In the present study, long-term exposure of neonatal rat astrocyte cultures to CSH resulted in the formation of large spherical, PAS-positive cytoplasmic inclusions which are highly reminiscent of, if not identical to, human CA. As in the case of human CA and Gomori-positive astrocyte granules, the CSH-induced CA-like inclusions exhibit non-enzymatic peroxidase activity and consistent immunolabelling with antibodies directed against the mitochondrial protein, sulphite oxidase. Taken together, our findings suggest that progressive mitochondrial damage and macroautophagy play an important role in the biogenesis of CA (and Gomori-positive granules) in astrocytes of the ageing periventricular brain.
Sankara P Sarma - One of the best experts on this subject based on the ideXlab platform.
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Corpora amylacea in mesial temporal lobe epilepsy clinico pathological correlations
Epilepsy Research, 2007Co-Authors: Ashalatha Radhakrishnan, V V Radhakrishnan, Kurupath Radhakrishnan, Paramban Rappai Mary, Chandrasekharan Kesavadas, Aley Alexander, Sankara P SarmaAbstract:Summary Purpose To investigate the electro-clinical significance of premature accumulation of Corpora amylacea (CoA) in the resected hippocampus of patients with medically refractory mesial temporal lobe epilepsy with hippocampal sclerosis (MTLE-HS). Methods We compared the clinical and EEG characteristics, and post-operative seizure outcome of 373 (mean age 29.4 years, range 7–55 years) surgically treated MTLE-HS patients with (MTLE-HS-CoA + , n =129 [34.5%]) and without (MTLE-HS-CoA − , n =244 [65.5%]) CoA. Results Age at surgery was significantly higher and duration of epilepsy before surgery was significantly longer for MTLE-HS-CoA + patients compared to MTLE-HS-CoA − patients. Although the distribution of interictal epileptiform EEG abnormalities did not differ, type 1 ictal EEG pattern was more frequent in MTLE-HS-CoA + patients. Among the 21 patients with major interictal psychosis detected prior to epilepsy surgery, 19 (90.5%) belonged to MTLE-HS-CoA + group. Schizophrenia-like psychosis was most prevalent. The post-operative seizure-free outcome was comparable, but significantly more MTLE-HS-CoA − patients were free of antiepileptic drugs. Conclusions Overall, our observations support the hypothesis that the pathological process in MTLE-HS is progressive. MTLE-HS-CoA + patients are predisposed to increased psychiatric morbidity. In vivo detection of hippocampal CoA accumulation in the future will help us to understand the neurobiological significance of this phenomenon.
Kurupath Radhakrishnan - One of the best experts on this subject based on the ideXlab platform.
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Corpora amylacea in mesial temporal lobe epilepsy clinico pathological correlations
Epilepsy Research, 2007Co-Authors: Ashalatha Radhakrishnan, V V Radhakrishnan, Kurupath Radhakrishnan, Paramban Rappai Mary, Chandrasekharan Kesavadas, Aley Alexander, Sankara P SarmaAbstract:Summary Purpose To investigate the electro-clinical significance of premature accumulation of Corpora amylacea (CoA) in the resected hippocampus of patients with medically refractory mesial temporal lobe epilepsy with hippocampal sclerosis (MTLE-HS). Methods We compared the clinical and EEG characteristics, and post-operative seizure outcome of 373 (mean age 29.4 years, range 7–55 years) surgically treated MTLE-HS patients with (MTLE-HS-CoA + , n =129 [34.5%]) and without (MTLE-HS-CoA − , n =244 [65.5%]) CoA. Results Age at surgery was significantly higher and duration of epilepsy before surgery was significantly longer for MTLE-HS-CoA + patients compared to MTLE-HS-CoA − patients. Although the distribution of interictal epileptiform EEG abnormalities did not differ, type 1 ictal EEG pattern was more frequent in MTLE-HS-CoA + patients. Among the 21 patients with major interictal psychosis detected prior to epilepsy surgery, 19 (90.5%) belonged to MTLE-HS-CoA + group. Schizophrenia-like psychosis was most prevalent. The post-operative seizure-free outcome was comparable, but significantly more MTLE-HS-CoA − patients were free of antiepileptic drugs. Conclusions Overall, our observations support the hypothesis that the pathological process in MTLE-HS is progressive. MTLE-HS-CoA + patients are predisposed to increased psychiatric morbidity. In vivo detection of hippocampal CoA accumulation in the future will help us to understand the neurobiological significance of this phenomenon.
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Corpora amylacea in mesial temporal lobe epilepsy: clinico-pathological correlations.
Epilepsy research, 2007Co-Authors: Ashalatha Radhakrishnan, V V Radhakrishnan, Kurupath Radhakrishnan, Paramban Rappai Mary, Chandrasekharan Kesavadas, Aley Alexander, P Sankara SarmaAbstract:To investigate the electro-clinical significance of premature accumulation of Corpora amylacea (CoA) in the resected hippocampus of patients with medically refractory mesial temporal lobe epilepsy with hippocampal sclerosis (MTLE-HS). We compared the clinical and EEG characteristics, and post-operative seizure outcome of 373 (mean age 29.4 years, range 7-55 years) surgically treated MTLE-HS patients with (MTLE-HS-CoA(+), n=129 [34.5%]) and without (MTLE-HS-CoA(-), n=244 [65.5%]) CoA. Age at surgery was significantly higher and duration of epilepsy before surgery was significantly longer for MTLE-HS-CoA(+) patients compared to MTLE-HS-CoA(-) patients. Although the distribution of interictal epileptiform EEG abnormalities did not differ, type 1 ictal EEG pattern was more frequent in MTLE-HS-CoA(+) patients. Among the 21 patients with major interictal psychosis detected prior to epilepsy surgery, 19 (90.5%) belonged to MTLE-HS-CoA(+) group. Schizophrenia-like psychosis was most prevalent. The post-operative seizure-free outcome was comparable, but significantly more MTLE-HS-CoA(-) patients were free of antiepileptic drugs. Overall, our observations support the hypothesis that the pathological process in MTLE-HS is progressive. MTLE-HS-CoA(+) patients are predisposed to increased psychiatric morbidity. In vivo detection of hippocampal CoA accumulation in the future will help us to understand the neurobiological significance of this phenomenon.
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the significance of Corpora amylacea in mesial temporal lobe epilepsy
Neurology India, 2003Co-Authors: P J Cherian, V V Radhakrishnan, Kurupath RadhakrishnanAbstract:Temporal lobe epilepsy (TLE) associated with mesial temporal sclerosis (MTS), mesial TLE (MTLE), is the commonest medically refractory adult epilepsy syndrome. Corpora amylacea (CoA) have been shown to be a marker of MTS. We compared 9 patients with MTS who had dense deposition of CoA in their hippocampi with 25 patients with MTS who did not have CoA. The patients with CoA were significantly older and they showed a trend towards having a significantly longer duration of epilepsy. The postoperative seizure outcome at 2 years was not different in the 2 groups. Our results could indicate the progressive nature of the pathology of MTS, probably indicating excitotoxic damage due to recurrent seizures, but they need to be verified by clinicopathological correlation among a larger number of patients with MTLE.
Toshiaki Kubota - One of the best experts on this subject based on the ideXlab platform.
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Reduction in number of Corpora amylacea with advancing histological changes of glaucoma
Graefe's Archive for Clinical and Experimental Ophthalmology, 1993Co-Authors: Toshiaki Kubota, Gottfried O. H. NaumannAbstract:Retina and optic nerve of eyes enucleated due to an iris ring melanoma or a malignant melanoma of the ciliary body were studied to investigate the correlation between Corpora amylacea count and glaucoma stages. Ten eyes from ten patients [age: 62.3±13.0 (mean±SD); range 43-84 years] were used for the present study. Eight eyes had shown elevated intraocular pressure readings preoperatively. The Corpora amylacea count was correlated with histomorphometric changes of the intra- and parapapillary region. The Corpora amylacea count of the retinal ganglion cells decreased significantly with increased histological ratio of cup to disc, decreased retinal nerve fiber layer thickness, and increased optic cup depth. There was no significant correlation between the Corpora amylacea count of the bipolar cells and each histomorphometric datum. These results suggest that the Corpora amylacea of the retinal ganglion cells decrease in number with advancing histological changes of secondary glaucoma.
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Corpora amylacea in glaucomatous and non-glaucomatous optic nerve and retina
Graefe's Archive for Clinical and Experimental Ophthalmology, 1993Co-Authors: Toshiaki Kubota, Leonard M. Holbach, Gottfried O. H. NaumannAbstract:We studied the occurrence, location, and size of Corpora amylacea (CA) in periodic acid-Schiff (PAS)-stained histological sections of the retina and optic nerve. The glaucoma group included 48 blind eyes obtained from 48 patients (mean age: 64±13.9 years; range 26–83 years) with advanced secondary angle-closure glaucoma. The non-glaucomatous group consisted of 45 non-glaucomatous eyes from 45 patients (mean age: 62.1±12.2 years, range 34–78 years) suffering from malignant melanoma of the choroid, and six autopsy eyes obtained from young individuals (age range 2.5–29 years). The mean diameter of CA at the level of the retinal ganglion cells (retrolaminar: 10.92±5.15 µm, intralaminar: 10.97±5.04 µm, prelaminar: 9.17±4.53 µm, nerve fiber layer: 8.56±4.27 µm) was significantly larger ( P
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Corpora amylacea in glaucomatous and non glaucomatous optic nerve and retina
Graefes Archive for Clinical and Experimental Ophthalmology, 1993Co-Authors: Toshiaki Kubota, Leonard M. Holbach, Gottfried O. H. NaumannAbstract:We studied the occurrence, location, and size of Corpora amylacea (CA) in periodic acid-Schiff (PAS)-stained histological sections of the retina and optic nerve. The glaucoma group included 48 blind eyes obtained from 48 patients (mean age: 64±13.9 years; range 26–83 years) with advanced secondary angle-closure glaucoma. The non-glaucomatous group consisted of 45 non-glaucomatous eyes from 45 patients (mean age: 62.1±12.2 years, range 34–78 years) suffering from malignant melanoma of the choroid, and six autopsy eyes obtained from young individuals (age range 2.5–29 years). The mean diameter of CA at the level of the retinal ganglion cells (retrolaminar: 10.92±5.15 µm, intralaminar: 10.97±5.04 µm, prelaminar: 9.17±4.53 µm, nerve fiber layer: 8.56±4.27 µm) was significantly larger (P<0.0001; Wilcoxon-Mann-Whitney test) than their size at the level of the bipolar cells (inner plexiform layer: 3.79±1.30 µm). The count of CA in sections from non-glaucomatous subjects aged 2.5 to 78 years (45 eyes with malignant melanoma and 6 autopsy eyes) increased significantly (P<0.01) with advancing age. CA occurred significantly more often (P<0.0001) in eyes with melanoma (45.7±29.4 per section) than in eyes with glaucoma (4.7±6.9 per section). These results suggest that CA represent intraneuronal aging products that are diminished in eyes with end-stage glaucoma due to neuronal loss.
