The Experts below are selected from a list of 309 Experts worldwide ranked by ideXlab platform
Anthony Atala - One of the best experts on this subject based on the ideXlab platform.
-
reconstitution of human Corpus Cavernosum smooth muscle in vitro and in vivo
Tissue Engineering, 2002Co-Authors: R T Kershen, Robert B. Moreland, Robert J Krane, Anthony AtalaAbstract:A large number of congenital and acquired abnormalities of the genitalia would benefit from the availability of transplantable, autologous Corpus Cavernosum tissue for use in reconstructive procedures. We describe the results of preliminary experiments designed to determine the feasibility of using cultured human corporal smooth muscle cells seeded onto biodegradable polymer scaffolds for the formation of Corpus Cavernosum smooth muscle in vitro and in vivo. Primary cultures of human Corpus Cavernosum smooth muscle cells were derived from operative biopsies obtained during penile prosthesis implantation. Cells were characterized in vitro and seeded as a contiguous multilayered sheet onto polymers of non-woven polyglycolic acid. The seeded polymer constructs were then implanted subcutaneously in athymic mice. Animals were killed 7, 14, and 24 days after surgery and implants were examined via histology, immunocytochemistry, and Western blot analyses. Cultured cell multilayers were identified as smooth muscl...
-
Reconstitution of human Corpus Cavernosum smooth muscle in vitro and in vivo
Tissue Engineering, 2002Co-Authors: R T Kershen, R J Krane, J. J. Yoo, Robert B. Moreland, Anthony AtalaAbstract:A large number of congenital and acquired abnormalities of the genitalia would benefit from the availability of transplantable, autologous Corpus Cavernosum tissue for use in reconstructive procedures. We describe the results of preliminary experiments designed to determine the feasibility of using cultured human corporal smooth muscle cells seeded onto biodegradable polymer scaffolds for the formation of Corpus Cavernosum smooth muscle in vitro and in vivo. Primary cultures of human Corpus Cavernosum smooth muscle cells were derived from operative biopsies obtained during penile prosthesis implantation. Cells were characterized in vitro and seeded as a contiguous multilayered sheet onto polymers of non-woven polyglycolic acid. The seeded polymer constructs were then implanted subcutaneously in athymic mice. Animals were killed 7, 14, and 24 days after surgery and implants were examined via histology, immunocytochemistry, and Western blot analyses. Cultured cell multilayers were identified as smooth muscle before implantation via phase-contrast microscopy, immunocytochemistry and Western blot analyses. Retrieved implants from all time points demonstrated corporal smooth muscle tissue grossly, and histologically, at the time of sacrifice. Intact smooth muscle cell multilayers were observed growing along the surface of the polymers. There was evidence of early vascular ingrowth at the periphery of the implants by 7 days. By 24 days, there was evidence of polymer degradation. Maintenance of the smooth muscle phenotype in vivo was confirmed immunocytochemically and by Western blot analyses with antibodies to alpha-smooth muscle actin. This study provides evidence that cultured human corporal smooth muscle cells may be used in conjunction with biodegradable polymer scaffolds to create Corpus Cavernosum smooth muscle tissue in vitro and in vivo.
Ali R Mani - One of the best experts on this subject based on the ideXlab platform.
-
role of endogenous hydrogen sulfide in neurogenic relaxation of rat Corpus Cavernosum
Biochemical Pharmacology, 2012Co-Authors: Mehdi Ghasemi, K Moore, Ali R Mani, Ahmad Reza DehpourAbstract:Abstract Relaxation of Corpus Cavernosum during penile erection is mediated by a non-adrenergic non-cholinergic (NANC) neurotransmission and by the endothelium via the release of nitric oxide. Hydrogen sulfide (H 2 S) is an endogenous gaseous mediator which is a potent vasodilator and a neurotransmitter. This study was initiated to characterize the role of H 2 S in NANC neurogenic transmission in rat Corpus Cavernosum. The expression of H 2 S producing enzymes was assessed using RT-PCR as well as Western blotting and showed the expression of cystathionine γ-lyase (CSE) in rat corporal tissue. Homogenates from rat Corpus Cavernosum convert l -cysteine to H 2 S and this was partially inhibited by a CSE inhibitor, propargylglycine. Electrical stimulation of corporal tissue strips caused NANC relaxation. This neurogenic relaxation was significantly enhanced by inhibition of CSE by propargylglycine indicating that endogenously produced H 2 S may have a negative regulatory role in neurogenic relaxation of rat Corpus Cavernosum. To investigate this further we used physiologically relevant concentrations of exogenous NaHS, and showed that nanomolar concentrations could inhibit corporal relaxation induced by a nitroxyl (HNO) donor (Angeli's salt) but not with nitrosonium (NO + ) or NO donors. This suggests that an interaction between endogenously produced H 2 S and nitroxyl (HNO) might be involved in erectile function.
-
d serine modulates neurogenic relaxation in rat Corpus Cavernosum
Biochemical Pharmacology, 2010Co-Authors: Mehdi Ghasemi, Fatemeh Rezania, Jackie Lewin, K Moore, Ali R ManiAbstract:Abstract d -Serine, an endogenous co-agonist for the N-methyl- d -aspartate (NMDA) receptor in mammals, is synthesized from l -serine by serine racemase. Although much attention has been focused on the role of d -serine within the central nervous system, the physiological role of d -serine in peripheral nerves such as Corpus cavernosal nerves has not been investigated. The present study was aimed to study the expression, cellular localization and function of serine racemase/ d -serine system in isolated rat Corpus Cavernosum. Reverse transcription polymerase chain reaction (RT-PCR) and Western blot analysis showed the expression of serine racemase in rat Corpus Cavernosum. Immunogold electron microscopy demonstrated the cellular localization of serine racemase in the cavernosal nerves’ membrane of the tissue. The organ bath studies on isolated rat Corpus Cavernosum showed that d -serine increases the non-adrenergic non-cholinergic neurogenic relaxation of isolated rat Corpus Cavernosum in vitro . This effect of d -serine was inhibited by a variety of NMDA receptor antagonists (ketamine, MK 801 and ifenprodil), suggesting that NMDA receptors are involved in the effects of d -serine on the neurogenic relaxation of corporal tissue strips. These observations provide the first evidence for the role of d -serine in modulating the neurogenic relaxation of rat Corpus Cavernosum, and may open new therapeutic avenues for the treatment of impotence.
R T Kershen - One of the best experts on this subject based on the ideXlab platform.
-
reconstitution of human Corpus Cavernosum smooth muscle in vitro and in vivo
Tissue Engineering, 2002Co-Authors: R T Kershen, Robert B. Moreland, Robert J Krane, Anthony AtalaAbstract:A large number of congenital and acquired abnormalities of the genitalia would benefit from the availability of transplantable, autologous Corpus Cavernosum tissue for use in reconstructive procedures. We describe the results of preliminary experiments designed to determine the feasibility of using cultured human corporal smooth muscle cells seeded onto biodegradable polymer scaffolds for the formation of Corpus Cavernosum smooth muscle in vitro and in vivo. Primary cultures of human Corpus Cavernosum smooth muscle cells were derived from operative biopsies obtained during penile prosthesis implantation. Cells were characterized in vitro and seeded as a contiguous multilayered sheet onto polymers of non-woven polyglycolic acid. The seeded polymer constructs were then implanted subcutaneously in athymic mice. Animals were killed 7, 14, and 24 days after surgery and implants were examined via histology, immunocytochemistry, and Western blot analyses. Cultured cell multilayers were identified as smooth muscl...
-
Reconstitution of human Corpus Cavernosum smooth muscle in vitro and in vivo
Tissue Engineering, 2002Co-Authors: R T Kershen, R J Krane, J. J. Yoo, Robert B. Moreland, Anthony AtalaAbstract:A large number of congenital and acquired abnormalities of the genitalia would benefit from the availability of transplantable, autologous Corpus Cavernosum tissue for use in reconstructive procedures. We describe the results of preliminary experiments designed to determine the feasibility of using cultured human corporal smooth muscle cells seeded onto biodegradable polymer scaffolds for the formation of Corpus Cavernosum smooth muscle in vitro and in vivo. Primary cultures of human Corpus Cavernosum smooth muscle cells were derived from operative biopsies obtained during penile prosthesis implantation. Cells were characterized in vitro and seeded as a contiguous multilayered sheet onto polymers of non-woven polyglycolic acid. The seeded polymer constructs were then implanted subcutaneously in athymic mice. Animals were killed 7, 14, and 24 days after surgery and implants were examined via histology, immunocytochemistry, and Western blot analyses. Cultured cell multilayers were identified as smooth muscle before implantation via phase-contrast microscopy, immunocytochemistry and Western blot analyses. Retrieved implants from all time points demonstrated corporal smooth muscle tissue grossly, and histologically, at the time of sacrifice. Intact smooth muscle cell multilayers were observed growing along the surface of the polymers. There was evidence of early vascular ingrowth at the periphery of the implants by 7 days. By 24 days, there was evidence of polymer degradation. Maintenance of the smooth muscle phenotype in vivo was confirmed immunocytochemically and by Western blot analyses with antibodies to alpha-smooth muscle actin. This study provides evidence that cultured human corporal smooth muscle cells may be used in conjunction with biodegradable polymer scaffolds to create Corpus Cavernosum smooth muscle tissue in vitro and in vivo.
Robert B. Moreland - One of the best experts on this subject based on the ideXlab platform.
-
Expression of functional prostaglandin D (DP) receptors in human Corpus Cavernosum smooth muscle
International Journal of Impotence Research, 2002Co-Authors: Robert B. Moreland, A Nehra, H Albadawi, M T Watkins, I Goldstein, A M TraishAbstract:Prostaglandin D_2 (PGD_2) binds to specific G-protein coupled receptors (DP) and induces smooth muscle relaxation by stimulating the synthesis of intracellular cAMP. In this study, we examined the role of PGD_2 and DP receptors in regulating human penile smooth muscle contractility. We determined that human Corpus Cavernosum tissue and smooth muscle cells in culture expressed functional DP receptor and lipocalin-like prostaglandin D synthase by reverse-transcribed polymerase chain reaction (RT-PCR). Functional PGD synthase activity was confirmed by the synthesis of PGD_2 in human Corpus Cavernosum smooth muscle cells upon addition of exogenous arachidonic acid. Organ bath preparations of human Corpus Cavernosum tissue strips, contracted with phenylephrine, relaxed in a dose-dependent fashion to either PGD_2 or the DP selective agonist BW245C. Cultures of human Corpus Cavernosum smooth muscle cells treated with BW245C showed a two-fold increase in cAMP synthesis. These data are consistent with the expression of functional DP receptors in human Corpus Cavernosum. This suggests the presence of an intact prostanoid autocrine system that may play a role in regulating penile erectile function.
-
reconstitution of human Corpus Cavernosum smooth muscle in vitro and in vivo
Tissue Engineering, 2002Co-Authors: R T Kershen, Robert B. Moreland, Robert J Krane, Anthony AtalaAbstract:A large number of congenital and acquired abnormalities of the genitalia would benefit from the availability of transplantable, autologous Corpus Cavernosum tissue for use in reconstructive procedures. We describe the results of preliminary experiments designed to determine the feasibility of using cultured human corporal smooth muscle cells seeded onto biodegradable polymer scaffolds for the formation of Corpus Cavernosum smooth muscle in vitro and in vivo. Primary cultures of human Corpus Cavernosum smooth muscle cells were derived from operative biopsies obtained during penile prosthesis implantation. Cells were characterized in vitro and seeded as a contiguous multilayered sheet onto polymers of non-woven polyglycolic acid. The seeded polymer constructs were then implanted subcutaneously in athymic mice. Animals were killed 7, 14, and 24 days after surgery and implants were examined via histology, immunocytochemistry, and Western blot analyses. Cultured cell multilayers were identified as smooth muscl...
-
Reconstitution of human Corpus Cavernosum smooth muscle in vitro and in vivo
Tissue Engineering, 2002Co-Authors: R T Kershen, R J Krane, J. J. Yoo, Robert B. Moreland, Anthony AtalaAbstract:A large number of congenital and acquired abnormalities of the genitalia would benefit from the availability of transplantable, autologous Corpus Cavernosum tissue for use in reconstructive procedures. We describe the results of preliminary experiments designed to determine the feasibility of using cultured human corporal smooth muscle cells seeded onto biodegradable polymer scaffolds for the formation of Corpus Cavernosum smooth muscle in vitro and in vivo. Primary cultures of human Corpus Cavernosum smooth muscle cells were derived from operative biopsies obtained during penile prosthesis implantation. Cells were characterized in vitro and seeded as a contiguous multilayered sheet onto polymers of non-woven polyglycolic acid. The seeded polymer constructs were then implanted subcutaneously in athymic mice. Animals were killed 7, 14, and 24 days after surgery and implants were examined via histology, immunocytochemistry, and Western blot analyses. Cultured cell multilayers were identified as smooth muscle before implantation via phase-contrast microscopy, immunocytochemistry and Western blot analyses. Retrieved implants from all time points demonstrated corporal smooth muscle tissue grossly, and histologically, at the time of sacrifice. Intact smooth muscle cell multilayers were observed growing along the surface of the polymers. There was evidence of early vascular ingrowth at the periphery of the implants by 7 days. By 24 days, there was evidence of polymer degradation. Maintenance of the smooth muscle phenotype in vivo was confirmed immunocytochemically and by Western blot analyses with antibodies to alpha-smooth muscle actin. This study provides evidence that cultured human corporal smooth muscle cells may be used in conjunction with biodegradable polymer scaffolds to create Corpus Cavernosum smooth muscle tissue in vitro and in vivo.
Bruce S Malkowicz - One of the best experts on this subject based on the ideXlab platform.
-
idiopathic partial thrombosis of the Corpus Cavernosum
Urology, 2010Co-Authors: Rakesh P Patel, Phillip Mucksavage, Parvati Ramchandani, Philip M Hanno, Bruce S MalkowiczAbstract:We report the case of an idiopathic partial segmental thrombosis of the left Corpus Cavernosum. The diagnosis was made by physical examination and radiological imaging. The patient was treated conservatively with aspirin, and follow-up imaging at 3 and 7 months revealed mild persistent thrombosis. Erectile function was maintained at 1 year follow-up.
