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Yue Shi - One of the best experts on this subject based on the ideXlab platform.
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fingerprint efficacy study of the quaternary alkaloids in Corydalis yanhusuo
Journal of Ethnopharmacology, 2017Co-Authors: Huanyu Guan, Xiaoming Wang, Hong Sun, Wenting Tan, Xiaomei Luo, Yue ShiAbstract:Abstract Ethnopharmacological relevance Corydalis yanhusuo is a well-known Chinese herbal medicine that is commonly applied as an analgesic agent in clinic using for thousands of years. Resent research showed that the quaternary ammonium alkaloids from Corydalis yanhusuo have a significant effect on myocardial ischemia. However, the corresponding anti-myocardial ischemia components that represent the efficacy of the quaternary ammonium alkaloids have not been elucidated. Aim of the study Explore the anti-myocardial ischemia components of Corydalis yanhusuo and develop a method of quality control for Corydalis yanhusuo. Chemical fingerprints of quaternary ammonium alkaloids extracted from Corydalis yanhusuo samples from 37 different sources were identified using UPLC-Q-TOF MS. The protective effects of the 37 samples with respect to H9C2 cell hypoxia–reoxygenation were detected by MTT assays. The fingerprint–efficacy relationship between the chemical fingerprints and cardioprotection afforded by Corydalis yanhusuo was investigated using three chemometric methods. Results Because of their inherent differences in chemical compositions, the protective effects to H9C2 cell hypoxia–reoxygenation were different. The results of three chemometric methods showed that the source of the Corydalis yanhusuo has an important influence on both the chemical fingerprint and efficacy. In particular, dehydrocorybulbine, 13-methyldehydrocorydalmine, dehydrocorydaline, columbamine, and palmatine appear to be the main effective components for quality control of this TCM. Conclusion This work provides a general model of combination of UPLC and cardioprotection efficiency to study the fingerprint–efficacy relationship of Corydalis yanhusuo which can offer some references for detecting principal components of Corydalis yanhusuo on cardioprotection efficiency. Fingerprint–efficacy studies also provide a powerful method of quality control in Corydalis yanhusuo and other TCMs.
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Fingerprint–efficacy study of the quaternary alkaloids in Corydalis yanhusuo
Journal of ethnopharmacology, 2017Co-Authors: Li Qiuyue, Huanyu Guan, Xiaoming Wang, Hong Sun, Wenting Tan, Xiaomei Luo, Yue ShiAbstract:Abstract Ethnopharmacological relevance Corydalis yanhusuo is a well-known Chinese herbal medicine that is commonly applied as an analgesic agent in clinic using for thousands of years. Resent research showed that the quaternary ammonium alkaloids from Corydalis yanhusuo have a significant effect on myocardial ischemia. However, the corresponding anti-myocardial ischemia components that represent the efficacy of the quaternary ammonium alkaloids have not been elucidated. Aim of the study Explore the anti-myocardial ischemia components of Corydalis yanhusuo and develop a method of quality control for Corydalis yanhusuo. Chemical fingerprints of quaternary ammonium alkaloids extracted from Corydalis yanhusuo samples from 37 different sources were identified using UPLC-Q-TOF MS. The protective effects of the 37 samples with respect to H9C2 cell hypoxia–reoxygenation were detected by MTT assays. The fingerprint–efficacy relationship between the chemical fingerprints and cardioprotection afforded by Corydalis yanhusuo was investigated using three chemometric methods. Results Because of their inherent differences in chemical compositions, the protective effects to H9C2 cell hypoxia–reoxygenation were different. The results of three chemometric methods showed that the source of the Corydalis yanhusuo has an important influence on both the chemical fingerprint and efficacy. In particular, dehydrocorybulbine, 13-methyldehydrocorydalmine, dehydrocorydaline, columbamine, and palmatine appear to be the main effective components for quality control of this TCM. Conclusion This work provides a general model of combination of UPLC and cardioprotection efficiency to study the fingerprint–efficacy relationship of Corydalis yanhusuo which can offer some references for detecting principal components of Corydalis yanhusuo on cardioprotection efficiency. Fingerprint–efficacy studies also provide a powerful method of quality control in Corydalis yanhusuo and other TCMs.
Zhanying Hong - One of the best experts on this subject based on the ideXlab platform.
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comparative studies on pharmacokinetic fates of tetrahydropalmatine enantiomers in different chemical environments in rats
Chirality, 2008Co-Authors: Zhanying Hong, Mei Lin, Guorong Fan, Yifeng Chai, Xueping YinAbstract:Tetrahydropalmatine (THP) is the active component in Rhizoma Corydalis and the medicine Yuanhu-Baizhi (YB), which consists of Rhizoma Corydalis and Radix angelicae dahuricae. The aim of this work was to compare pharmacokinetic features of THP enantiomers in rats dosed with racemic THP (rac-THP), Rhizoma Corydalis, or YB extracts. A single dose of rac-THP (5 mg kg(-1)) or extracts of Rhizoma Corydalis and YB (both equivalent to 5 mg kg(-1) of rac-THP) was given orally to three groups of Sprague-Dawley rats, respectively. Blood samples were collected periodically and plasma concentrations of THP enantiomers were determined using an achiral-chiral high-performance liquid chromatographic (HPLC) method previously reported, with some modifications. The C(max) ratio (-/+) of THP was 2.91, 1.38, and 1.19, and the AUC(0 approximately infinity) ratio (-/+) of THP was 2.84, 1.50, and 1.35 in rats after dosed with rac-THP, extracts of Rhizoma Corydalis and YB, respectively. The mean AUC(0 approximately infinity) and C(max) of (+)-THP dosed with YB extracts were 0.652 +/- 0.30 microg h ml(-1) and 0.148 +/- 0.09 microg ml(-1), significantly higher (P < 0.05) than those dosed with rac-THP and Rhizoma Corydalis extracts. The mean AUC(0 approximately infinity) and T(max) of rac-THP dosed with YB extracts were 1.500 +/- 0.56 microg h ml(-1) and 2.12 +/- 1.1 h, significantly higher (P < 0.05) than those dosed with rac-THP or Rhizoma Corydalis extracts. These findings suggested the stereoselectivity in pharmacokinetics of THP enantiomers in rats was decreased when dosed in plant form, while the AUC(0 approximately infinity) of rac-THP increased when YB extracts were dosed, confirming the compatibility in drug combination of Rhizoma Corydalis and Radix angelicae dahuricae.
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qualitative and quantitative determination of ten alkaloids in traditional chinese medicine Corydalis yanhusuo w t wang by lc ms ms and lc dad
Journal of Pharmaceutical and Biomedical Analysis, 2007Co-Authors: Bo Ding, Guorong Fan, Tingting Zhou, Zhanying HongAbstract:Abstract An analytical method incorporating high-performance liquid chromatography (HPLC) with MS and UV-detection was developed for the qualitative and quantitative determination of alkaloids in Corydalis yanhusuo . Ten alkaloids, including seven tertiary alkaloids and three quaternary alkaloids, were identified by comparing their retention times, UV and MS spectra with those of authentic compounds. Furthermore, the collision-induced dissociations of the [M + H] + and [M] + ions were studied to clarify the MS behavior of the different types of alkaloids. In positive ion electrospray ionization tandem mass spectrometry (ESI-MS) all the tertiary alkaloids yielded prominent [M + H] + ions and quaternary alkaloids yielded prominent [M] + ions in the first order mass spectra. Fragments involving losses of H, CH 3 , CO, H 2 O and OCH 3 were observed in the MS/MS spectra. In addition, quantification of the 10 alkaloids in Corydalis yanhusuo from methanol and ethyl acetate extract of different origins were performed by this method, which provides a new tool for the assessment of quality of Corydalis yanhusuo preparations. The method provides the best sensitivity and specificity for characterization and quantitative determination of the alkaloids in Corydalis yanhusuo so far.
Sun Wei - One of the best experts on this subject based on the ideXlab platform.
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Study on processing techniques of Corydalis
Journal of Tianjin University of Traditional Chinese Medicine, 2009Co-Authors: Sun WeiAbstract:[Objective]To choice the most suitable processing conditions for Corydalis and to provide a standard processing procedure.[Methods]Orthogonal design was adopted to optimize the processing methods.The content of Tetrahydropalmatine and Dehydrocorydaline were the evaluation indexs. [Results]The optimized conditions of vinegar-fried Corydalis were fragmenting to 0.4～0.5cm,adding the vinegar quality ratio of 40%,mixing run for 2 hours and frying at the temprature of 150℃;The optimized conditions of liquor-fried Corydalis were fragmenting to 0.4～0.5cm,adding the liquor quality ratio of 20%,mixing run for 6 hours,and frying at the temprature of 120℃;The optimized conditions of vinegar-sauted Corydalis were fragmenting to 0.2～0.3cm,adding the vinegar quality ratio of 20%and the water ratio of 80%～120%and mixing run for 2 hours.[Conclusion]This three methods are reliable, simple,and have a good repeatability,there are positive meanings for Corydalis standardization.
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Efect of Different Processing on the Content of Tetrahydropalmatine in Rhizoma Corydalis
Journal of Tianjin University of Traditional Chinese Medicine, 2006Co-Authors: Sun WeiAbstract:[Objective] To explore the effect of different processing on the content of tetrahydropalmatine in Rhizoma Corydalis. [Methods] For the different processing products of Corydalis,HPLC method was used with chromatographic condition included: Thermo hypersil GOLD-C18 column,mobile phase of content determine is methanol-water(62∶38). The UV detector was set at 280 nm. [Results] The content of tetrahydropalmatine in the Rhizoma Corydlis roast with vinergar and rice wine is the hightest among all processed products,The recovery is 100.81% and RSD=2.76%,r=0.999 7. [Conclusion] The processing product with vinegar and rice wine can increase tetrahydropalmatine content of Rhizoma Corydalis.The boiling product with vinegar was a descent.
Lianxiu Jiang - One of the best experts on this subject based on the ideXlab platform.
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beneficial effects of the extract from Corydalis yanhusuo in rats with heart failure following myocardial infarction
Journal of Pharmacy and Pharmacology, 2010Co-Authors: Haiyun Ling, Lianxiu JiangAbstract:As indicated in ancient Chinese medical books, Corydalis yanhusuo has therapeutic effects on cardiovascular diseases. The analgesic effect of this plant has been fully elucidated, and l-tetrahydropalmatine has been shown to be the main active principle. The aim of this investigation was to evaluate its protective effects in a rat heart failure model. Rats were subjected to coronary artery ligation, and orally administered with ethanolic extract of Corydalis yanhusuo 50, 100, or 200 mg kg(-1) daily, from the 7th day after surgery. We measured cardiac function, plasma atrial natriuretic peptide (ANP), relative heart and lung weights, infarct size and ventricular dilatation after treatment for 8 weeks. Administration with Corydalis yanhusuo led to a significant reduction in infarct size and improvement in cardiac function as demonstrated by lower left ventricular end diastolic pressure (LVEDP) and elevated +/-dp/dt(max). We also found that Corydalis yanhusuo significantly reduced left ventricular (LV)/body weight ratio, lung/body weight ratio and significantly inhibited neurohormonal activation. Taken together, this study indicated that Corydalis yanhusuo exerted salutary effects on heart failure induced by myocardial infarction in rats.
Huanyu Guan - One of the best experts on this subject based on the ideXlab platform.
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fingerprint efficacy study of the quaternary alkaloids in Corydalis yanhusuo
Journal of Ethnopharmacology, 2017Co-Authors: Huanyu Guan, Xiaoming Wang, Hong Sun, Wenting Tan, Xiaomei Luo, Yue ShiAbstract:Abstract Ethnopharmacological relevance Corydalis yanhusuo is a well-known Chinese herbal medicine that is commonly applied as an analgesic agent in clinic using for thousands of years. Resent research showed that the quaternary ammonium alkaloids from Corydalis yanhusuo have a significant effect on myocardial ischemia. However, the corresponding anti-myocardial ischemia components that represent the efficacy of the quaternary ammonium alkaloids have not been elucidated. Aim of the study Explore the anti-myocardial ischemia components of Corydalis yanhusuo and develop a method of quality control for Corydalis yanhusuo. Chemical fingerprints of quaternary ammonium alkaloids extracted from Corydalis yanhusuo samples from 37 different sources were identified using UPLC-Q-TOF MS. The protective effects of the 37 samples with respect to H9C2 cell hypoxia–reoxygenation were detected by MTT assays. The fingerprint–efficacy relationship between the chemical fingerprints and cardioprotection afforded by Corydalis yanhusuo was investigated using three chemometric methods. Results Because of their inherent differences in chemical compositions, the protective effects to H9C2 cell hypoxia–reoxygenation were different. The results of three chemometric methods showed that the source of the Corydalis yanhusuo has an important influence on both the chemical fingerprint and efficacy. In particular, dehydrocorybulbine, 13-methyldehydrocorydalmine, dehydrocorydaline, columbamine, and palmatine appear to be the main effective components for quality control of this TCM. Conclusion This work provides a general model of combination of UPLC and cardioprotection efficiency to study the fingerprint–efficacy relationship of Corydalis yanhusuo which can offer some references for detecting principal components of Corydalis yanhusuo on cardioprotection efficiency. Fingerprint–efficacy studies also provide a powerful method of quality control in Corydalis yanhusuo and other TCMs.
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Fingerprint–efficacy study of the quaternary alkaloids in Corydalis yanhusuo
Journal of ethnopharmacology, 2017Co-Authors: Li Qiuyue, Huanyu Guan, Xiaoming Wang, Hong Sun, Wenting Tan, Xiaomei Luo, Yue ShiAbstract:Abstract Ethnopharmacological relevance Corydalis yanhusuo is a well-known Chinese herbal medicine that is commonly applied as an analgesic agent in clinic using for thousands of years. Resent research showed that the quaternary ammonium alkaloids from Corydalis yanhusuo have a significant effect on myocardial ischemia. However, the corresponding anti-myocardial ischemia components that represent the efficacy of the quaternary ammonium alkaloids have not been elucidated. Aim of the study Explore the anti-myocardial ischemia components of Corydalis yanhusuo and develop a method of quality control for Corydalis yanhusuo. Chemical fingerprints of quaternary ammonium alkaloids extracted from Corydalis yanhusuo samples from 37 different sources were identified using UPLC-Q-TOF MS. The protective effects of the 37 samples with respect to H9C2 cell hypoxia–reoxygenation were detected by MTT assays. The fingerprint–efficacy relationship between the chemical fingerprints and cardioprotection afforded by Corydalis yanhusuo was investigated using three chemometric methods. Results Because of their inherent differences in chemical compositions, the protective effects to H9C2 cell hypoxia–reoxygenation were different. The results of three chemometric methods showed that the source of the Corydalis yanhusuo has an important influence on both the chemical fingerprint and efficacy. In particular, dehydrocorybulbine, 13-methyldehydrocorydalmine, dehydrocorydaline, columbamine, and palmatine appear to be the main effective components for quality control of this TCM. Conclusion This work provides a general model of combination of UPLC and cardioprotection efficiency to study the fingerprint–efficacy relationship of Corydalis yanhusuo which can offer some references for detecting principal components of Corydalis yanhusuo on cardioprotection efficiency. Fingerprint–efficacy studies also provide a powerful method of quality control in Corydalis yanhusuo and other TCMs.
