The Experts below are selected from a list of 2733 Experts worldwide ranked by ideXlab platform
Tingting Niu - One of the best experts on this subject based on the ideXlab platform.
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simultaneous determination of scutellarin and Tetrahydropalmatine of deng yan granule in rat plasma by uflc ms ms and its application to a pharmacokinetic study
Journal of Chromatography B, 2014Co-Authors: Xiurong Zhang, Jiao Guan, Heyun Zhu, Tingting NiuAbstract:Deng-yan granule, consisting of Herba Erigerontis Breviscapi, Rhizoma Corydalis Yanhusuo and Radix Astragali Mongolici, is a widely used Traditional Chinese Medicine preparation for treatment of coronary heart disease. Scutellarin and Tetrahydropalmatine are main active constituents in Herba Erigerontis Breviscapi and Rhizoma Corydalis Yanhusuo, and have been used as marker components for quality control of Deng-yan preparations. In order to make good and rational use of Deng-yan granule in the future, a rapid, sensitive and high throughput ultra-fast liquid chromatography with tandem mass spectrometry (UFLC-MS/MS) method was developed for the simultaneous determination of scutellarin and Tetrahydropalmatine in rat plasma using rutin as internal standard (IS). The plasma samples were extracted by liquid-liquid extraction with ethyl acetate after acidification and separated on a Shim-pack XR-ODS C18 column (75mm×3.0mm, 2.2μm) with a mobile phase consisting of methanol-0.1% formic acid water (50:50, v/v) at a flow rate of 0.4mL/min. Mass spectrometric detection was conducted on an API 3200 QTRAP mass spectrometry equipped with electrospray ionization source in positive ionization mode. Quantification was performed using multiple reaction monitoring (MRM) by monitoring the fragmentation of m/z 463.2→287.1 for scutellarin, m/z 356.1→192.1 for Tetrahydropalmatine and m/z 611.2→303.2 for IS, respectively. The linear range was 10-5000ng/mL for both scutellarin and Tetrahydropalmatine with lower limit of quantitation (LLOQ) of 10ng/mL. The intra- and inter-day precisions were below 12.2% for scutellarin and below 9.7% for Tetrahydropalmatine in terms of relative standard deviation (RSD), and the accuracy was within ±9.1% for scutellarin and within ±11.2% for Tetrahydropalmatine in terms of relative error (RE). Extraction recovery, matrix effect and stability were satisfactory in rat plasma. The validated method was successfully applied to a pharmacokinetic study of scutellarin and Tetrahydropalmatine after oral administration of Deng-yan granule to rats.
Baoxian Ye - One of the best experts on this subject based on the ideXlab platform.
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a simple and sensitive method for determination of Tetrahydropalmatine based on a new voltammetric sensor
Talanta, 2016Co-Authors: Qinqin Wang, Gaiping Li, Kai Sheng, Lu Wang, Baoxian YeAbstract:Abstract In this paper, a simple, low cost, highly sensitive voltammetric method for determination of Tetrahydropalmatine was described, which was based on a new electrochemical sensor, poly (L-glutamic acid) functionalized electrochemical reduction graphene oxide modified glassy carbon electrode. The electrochemical behavior of Tetrahydropalmatine was investigated in detail and some dynamic parameters of electrode process were calculated for the first time. Due to large surface area and good conductivity of proposed sensor, a wider linear range (3.0×10 −7 –2.0×10 −5 mol L −1 ) and lower detection limit (1.0×10 −7 mol L −1 ) were achieved for determination of Tetrahydropalmatine. Meanwhile, the proposed method was used in determination of Tetrahydropalmatine in Rhizoma Corydalis with well results.
Lu Wang - One of the best experts on this subject based on the ideXlab platform.
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a simple and sensitive method for determination of Tetrahydropalmatine based on a new voltammetric sensor
Talanta, 2016Co-Authors: Qinqin Wang, Gaiping Li, Kai Sheng, Lu Wang, Baoxian YeAbstract:Abstract In this paper, a simple, low cost, highly sensitive voltammetric method for determination of Tetrahydropalmatine was described, which was based on a new electrochemical sensor, poly (L-glutamic acid) functionalized electrochemical reduction graphene oxide modified glassy carbon electrode. The electrochemical behavior of Tetrahydropalmatine was investigated in detail and some dynamic parameters of electrode process were calculated for the first time. Due to large surface area and good conductivity of proposed sensor, a wider linear range (3.0×10 −7 –2.0×10 −5 mol L −1 ) and lower detection limit (1.0×10 −7 mol L −1 ) were achieved for determination of Tetrahydropalmatine. Meanwhile, the proposed method was used in determination of Tetrahydropalmatine in Rhizoma Corydalis with well results.
Xiaohui Pu - One of the best experts on this subject based on the ideXlab platform.
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simultaneous determination of Tetrahydropalmatine protopine and palmatine in rat plasma by lc esi ms and its application to a pharmacokinetic study
Journal of Pharmaceutical and Biomedical Analysis, 2009Co-Authors: Yongjun Wang, Zhonggui He, Xinyu Chang, Xiaohui PuAbstract:Abstract A sensitive and specific liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS) method has been developed and validated for the simultaneous quantification of Tetrahydropalmatine, protopine and palmatine in rat plasma using phenacetin as the internal standard (IS). Two hundred microliters plasma samples were extracted by dichloromethane under a strong basic condition. The analytes were separated by a C18 column and detected with a single quadrupole mass spectrometer. The used mobile phase was acetonitrile–water (40:60, v/v) containing 5 mM ammonium acetate and 0.2% glacial acetic acid. Detection was carried out by positive electrospray ionization in selected ion reaction (SIR) mode at m/z 356.6 for Tetrahydropalmatine, 354.6 for protopine, 352.6 for palmatine and 180.4 for the IS, respectively. The method was validated over the concentration range of 1.00–500 ng mL−1 and the lower limit of quantification (LLOQ) was 1.00 ng mL−1 for all three analytes. The intra- and inter-day precision values were less than 9% relative standard deviation (R.S.D.), and the relative error ranged from −7.4 to 4.8%. The extraction recoveries were on average 91.42% for Tetrahydropalmatine, 84.75% for protopine, 57.26% for palmatine, and 83.18% for IS. The validated method was successfully applied to a pharmacokinetic study of Tetrahydropalmatine, protopine and palmatine in rats after oral administration of Rhizoma Corydalis Decumbentis extract.
Xiurong Zhang - One of the best experts on this subject based on the ideXlab platform.
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simultaneous determination of scutellarin and Tetrahydropalmatine of deng yan granule in rat plasma by uflc ms ms and its application to a pharmacokinetic study
Journal of Chromatography B, 2014Co-Authors: Xiurong Zhang, Jiao Guan, Heyun Zhu, Tingting NiuAbstract:Deng-yan granule, consisting of Herba Erigerontis Breviscapi, Rhizoma Corydalis Yanhusuo and Radix Astragali Mongolici, is a widely used Traditional Chinese Medicine preparation for treatment of coronary heart disease. Scutellarin and Tetrahydropalmatine are main active constituents in Herba Erigerontis Breviscapi and Rhizoma Corydalis Yanhusuo, and have been used as marker components for quality control of Deng-yan preparations. In order to make good and rational use of Deng-yan granule in the future, a rapid, sensitive and high throughput ultra-fast liquid chromatography with tandem mass spectrometry (UFLC-MS/MS) method was developed for the simultaneous determination of scutellarin and Tetrahydropalmatine in rat plasma using rutin as internal standard (IS). The plasma samples were extracted by liquid-liquid extraction with ethyl acetate after acidification and separated on a Shim-pack XR-ODS C18 column (75mm×3.0mm, 2.2μm) with a mobile phase consisting of methanol-0.1% formic acid water (50:50, v/v) at a flow rate of 0.4mL/min. Mass spectrometric detection was conducted on an API 3200 QTRAP mass spectrometry equipped with electrospray ionization source in positive ionization mode. Quantification was performed using multiple reaction monitoring (MRM) by monitoring the fragmentation of m/z 463.2→287.1 for scutellarin, m/z 356.1→192.1 for Tetrahydropalmatine and m/z 611.2→303.2 for IS, respectively. The linear range was 10-5000ng/mL for both scutellarin and Tetrahydropalmatine with lower limit of quantitation (LLOQ) of 10ng/mL. The intra- and inter-day precisions were below 12.2% for scutellarin and below 9.7% for Tetrahydropalmatine in terms of relative standard deviation (RSD), and the accuracy was within ±9.1% for scutellarin and within ±11.2% for Tetrahydropalmatine in terms of relative error (RE). Extraction recovery, matrix effect and stability were satisfactory in rat plasma. The validated method was successfully applied to a pharmacokinetic study of scutellarin and Tetrahydropalmatine after oral administration of Deng-yan granule to rats.
