Corynebacterium Pseudotuberculosis

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Vasco Azevedo - One of the best experts on this subject based on the ideXlab platform.

Sharon J. Spier - One of the best experts on this subject based on the ideXlab platform.

  • Whole-Genome Sequence of Corynebacterium Pseudotuberculosis Strain 226, Isolated from the Abscess of a Goat in California
    Genome announcements, 2016
    Co-Authors: Larissa M. Dias, Luis C Guimaraes, Jorianne Thyeska Castro Alves, Adonney A. O. Veras, Judy M. Edman, Sharon J. Spier, Flávia S. Rocha, Rafael A. Baraúna, Rommel Thiago Jucá Ramos
    Abstract:

    Corynebacterium Pseudotuberculosis is the etiological agent of a caseous lymphadenitis disease. Herein, we present the first complete genome sequencing of C. Pseudotuberculosis strain 226, isolated from an abscess of the sub-iliac lymph node of a goat from California (USA). The genome contains 2,138 coding sequences (CDSs), 12 rRNAs, 49 tRNAs, and 72 pseudogenes.

  • Minimum Inhibitory Concentrations of Equine Corynebacterium Pseudotuberculosis Isolates (1996–2012)
    Journal of veterinary internal medicine, 2015
    Co-Authors: D. M. Rhodes, K G Magdesian, Barbara A. Byrne, Philip H. Kass, Judy M. Edman, Sharon J. Spier
    Abstract:

    Background Few studies report the minimum inhibitory concentrations for antimicrobials against equine Corynebacterium Pseudotuberculosis isolates. Hypothesis/Objectives To evaluate trends in the in vitro activities of 20 antimicrobials against equine Corynebacterium Pseudotuberculosis isolates from 1996 to 2012 and to determine if a relationship exists between the minimum inhibitory concentration (MIC) and location of the abscess. Animals Corynebacterium Pseudotuberculosis isolates from 196 horses with naturally occurring disease. Methods Retrospective and cross-sectional design. Medical records were reviewed to obtain clinical and MIC data. Minimum inhibitory concentrations were determined by the microdilution technique. The MIC results over 3 periods were compared (1996–2001, 2002–2006, 2007–2012). Results The MIC90 values for clinically relevant antimicrobials were as follows: chloramphenicol ≤4 μg/mL, enrofloxacin ≤0.25 μg/mL, gentamicin ≤1 μg/mL, penicillin =0.25 μg/mL, rifampin ≤1 μg/mL, tetracycline ≤2 μg/mL, trimethoprim-sulfamethoxazole (TMS) ≤0.5 μg/mL, ceftiofur =2 μg/mL, and doxycycline ≤2 μg/mL. There were no significant changes in MIC results over the study period. There was no relationship between MIC patterns and abscess location. Conclusions and Clinical Importance The MIC50 and MIC90 values of antimicrobials evaluated in this study for equine isolates of C. Pseudotuberculosis did not vary over time. Abscess location was not associated with different MIC patterns in cultured isolates. Several commonly used antimicrobials are active in vitro against C. Pseudotuberculosis in vitro.

  • minimum inhibitory concentrations of equine Corynebacterium Pseudotuberculosis isolates 1996 2012
    Journal of Veterinary Internal Medicine, 2015
    Co-Authors: D. M. Rhodes, K G Magdesian, Barbara A. Byrne, Philip H. Kass, Judy M. Edman, Sharon J. Spier
    Abstract:

    Background Few studies report the minimum inhibitory concentrations for antimicrobials against equine Corynebacterium Pseudotuberculosis isolates. Hypothesis/Objectives To evaluate trends in the in vitro activities of 20 antimicrobials against equine Corynebacterium Pseudotuberculosis isolates from 1996 to 2012 and to determine if a relationship exists between the minimum inhibitory concentration (MIC) and location of the abscess. Animals Corynebacterium Pseudotuberculosis isolates from 196 horses with naturally occurring disease. Methods Retrospective and cross-sectional design. Medical records were reviewed to obtain clinical and MIC data. Minimum inhibitory concentrations were determined by the microdilution technique. The MIC results over 3 periods were compared (1996–2001, 2002–2006, 2007–2012). Results The MIC90 values for clinically relevant antimicrobials were as follows: chloramphenicol ≤4 μg/mL, enrofloxacin ≤0.25 μg/mL, gentamicin ≤1 μg/mL, penicillin =0.25 μg/mL, rifampin ≤1 μg/mL, tetracycline ≤2 μg/mL, trimethoprim-sulfamethoxazole (TMS) ≤0.5 μg/mL, ceftiofur =2 μg/mL, and doxycycline ≤2 μg/mL. There were no significant changes in MIC results over the study period. There was no relationship between MIC patterns and abscess location. Conclusions and Clinical Importance The MIC50 and MIC90 values of antimicrobials evaluated in this study for equine isolates of C. Pseudotuberculosis did not vary over time. Abscess location was not associated with different MIC patterns in cultured isolates. Several commonly used antimicrobials are active in vitro against C. Pseudotuberculosis in vitro.

  • Pilot immunization of mice infected with an equine strain of Corynebacterium Pseudotuberculosis.
    Veterinary therapeutics : research in applied veterinary medicine, 2010
    Co-Authors: Jennifer K. Gorman, Mourad W. Gabriel, Nigel J Maclachlan, Nathan C. Nieto, Janet E Foley, Sharon J. Spier
    Abstract:

    This pilot study evaluated protection of an equine autogenous bacterin-toxoid vaccine against Corynebacterium Pseudotuberculosis infection. Twenty-four BALB/c mice were inoculated with two doses of bacterin-toxoid vaccine or two injections of a placebo. Clinical, microbiologic, and pathologic outcomes were assessed after intradermal infection with one of two equine-origin C. Pseudotuberculosis strains. Mice receiving bacterin-toxoid from fast-growing C. Pseudotuberculosis showed significant protection from challenge infection, as evidenced by a higher survival rate, fewer gross and histopathologic lesions, and lower bacterial levels on culture. Successful protection via a vaccine against equine internal abscesses might provide supplementary management options against an important, potentially fatal disease.

  • Respiratory Disease Caused by Corynebacterium Pseudotuberculosis
    Clinical Techniques in Equine Practice, 2006
    Co-Authors: Suzanne M. Pratt, Sharon J. Spier, Betsy Vaughan, Mary Beth Withcomb, Megan R. Uerling, W. David Wilson
    Abstract:

    Corynebacterium Pseudotuberculosis is a common cause of external abscesses in horses. Approximately 8% of affected horses develop internal infection, which is associated with increased mortality and expense. Respiratory disease caused by C. Pseudotuberculosis occurs in approximately 40% of horses that develop internal C. Pseudotuberculosis infection. The lower respiratory tract may be the only organ system affected and infection can manifest as pneumonia or pleuropneumonia. Approximately half of the horses that develop C. Pseudotuberculosis lower respiratory tract infection have concurrent abdominal organ involvement. The liver is the site most commonly affected, although renal and splenic involvement also occurs. Abdominal ultrasound is an important diagnostic technique to specifically identify affected abdominal organs. With early diagnosis, a high proportion of horses with internal C. Pseudotuberculosis infection respond well to long-term antimicrobial therapy; however, pregnant mares are at risk for fetal loss.

Marcus Vinicius Canário Viana - One of the best experts on this subject based on the ideXlab platform.

Rommel Thiago Jucá Ramos - One of the best experts on this subject based on the ideXlab platform.

Artur Silva - One of the best experts on this subject based on the ideXlab platform.