The Experts below are selected from a list of 264 Experts worldwide ranked by ideXlab platform
Roberto Montes De Oca-jiménez - One of the best experts on this subject based on the ideXlab platform.
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Identification and molecular characterization of Corynebacterium Xerosis isolated from a sheep cutaneous abscess: first case report in Mexico
BMC research notes, 2016Co-Authors: Fernando Hernández-león, Jorge Acosta-dibarrat, Juan Carlos Vázquez-chagoyán, Pomposo Fernández Rosas, Roberto Montes De Oca-jiménezAbstract:Background Corynebacterium Xerosis is a commensal organism found in skin and mucous membranes of humans. It is considered an unusual pathogen, and it is rarely found in human and animal clinical samples. Here we describe the isolation of C. Xerosis from a 4-months-old Pelifolk lamb located in Tesistan, central western Mexico. This microorganism should be considered for differential diagnosis in cutaneous abscessed lesions in sheep, as it represents a zoonotic risk factor for human infection in sheep farms.
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Identification and molecular characterization of Corynebacterium Xerosis isolated from a sheep cutaneous abscess: first case report in Mexico
BMC Research Notes, 2016Co-Authors: Fernando Hernández-león, Jorge Acosta-dibarrat, Juan Carlos Vázquez-chagoyán, Pomposo Fernández Rosas, Roberto Montes De Oca-jiménezAbstract:Background Corynebacterium Xerosis is a commensal organism found in skin and mucous membranes of humans. It is considered an unusual pathogen, and it is rarely found in human and animal clinical samples. Here we describe the isolation of C. Xerosis from a 4-months-old Pelifolk lamb located in Tesistán, central western Mexico. This microorganism should be considered for differential diagnosis in cutaneous abscessed lesions in sheep, as it represents a zoonotic risk factor for human infection in sheep farms. Case presentation The animal exhibited a hard-consistency, 5 cm diameter abscess, without drainage, in the neck. The presumptive clinical diagnosis was caseous lymphadenitis, caused by Corynebacterium pseudotuberculosis . Samples were obtained by puncture and cultured in 8 % sheep blood agar under microaerophilic conditions. Colonies were non-haemolytic, brown-yellowish and showed microscopic and biochemical features similar to C. pseudotuberculosis , except for the urea test. A multiplex-PCR for the amplification of partial sequences of the pld , rpoB and intergenic fragment from 16S to 23S genes suggested that isolate could be C. Xerosis, which was later confirmed by sequencing analysis of the rpoB gene. Conclusions This study shows for the first time isolation and molecular characterization of C. Xerosis from a clinical sample of an ovine cutaneous abscess in Mexico. This finding highlights the need for differential diagnosis of this pathogen in ovine skin abscesses, as well as epidemiological and control studies of this pathogen in sheep farms.
Fernando Hernández-león - One of the best experts on this subject based on the ideXlab platform.
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Identification and molecular characterization of Corynebacterium Xerosis isolated from a sheep cutaneous abscess: first case report in Mexico
BMC research notes, 2016Co-Authors: Fernando Hernández-león, Jorge Acosta-dibarrat, Juan Carlos Vázquez-chagoyán, Pomposo Fernández Rosas, Roberto Montes De Oca-jiménezAbstract:Background Corynebacterium Xerosis is a commensal organism found in skin and mucous membranes of humans. It is considered an unusual pathogen, and it is rarely found in human and animal clinical samples. Here we describe the isolation of C. Xerosis from a 4-months-old Pelifolk lamb located in Tesistan, central western Mexico. This microorganism should be considered for differential diagnosis in cutaneous abscessed lesions in sheep, as it represents a zoonotic risk factor for human infection in sheep farms.
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Identification and molecular characterization of Corynebacterium Xerosis isolated from a sheep cutaneous abscess: first case report in Mexico
BMC Research Notes, 2016Co-Authors: Fernando Hernández-león, Jorge Acosta-dibarrat, Juan Carlos Vázquez-chagoyán, Pomposo Fernández Rosas, Roberto Montes De Oca-jiménezAbstract:Background Corynebacterium Xerosis is a commensal organism found in skin and mucous membranes of humans. It is considered an unusual pathogen, and it is rarely found in human and animal clinical samples. Here we describe the isolation of C. Xerosis from a 4-months-old Pelifolk lamb located in Tesistán, central western Mexico. This microorganism should be considered for differential diagnosis in cutaneous abscessed lesions in sheep, as it represents a zoonotic risk factor for human infection in sheep farms. Case presentation The animal exhibited a hard-consistency, 5 cm diameter abscess, without drainage, in the neck. The presumptive clinical diagnosis was caseous lymphadenitis, caused by Corynebacterium pseudotuberculosis . Samples were obtained by puncture and cultured in 8 % sheep blood agar under microaerophilic conditions. Colonies were non-haemolytic, brown-yellowish and showed microscopic and biochemical features similar to C. pseudotuberculosis , except for the urea test. A multiplex-PCR for the amplification of partial sequences of the pld , rpoB and intergenic fragment from 16S to 23S genes suggested that isolate could be C. Xerosis, which was later confirmed by sequencing analysis of the rpoB gene. Conclusions This study shows for the first time isolation and molecular characterization of C. Xerosis from a clinical sample of an ovine cutaneous abscess in Mexico. This finding highlights the need for differential diagnosis of this pathogen in ovine skin abscesses, as well as epidemiological and control studies of this pathogen in sheep farms.
Claude Bollet - One of the best experts on this subject based on the ideXlab platform.
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Corynebacterium hansenii sp. nov., an α-glucosidase-negative bacterium related to Corynebacterium Xerosis
International Journal of Systematic and Evolutionary Microbiology, 2007Co-Authors: François N.r. Renaud, Philippe Riegel, Dominique Aubel, Alain Le Coustumier, Nathalie Wilhem, Claude Bollet, Jean FreneyAbstract:A novel strain, C-138T, belonging to the genus Corynebacterium was isolated from a severe thigh liposarcoma infection and its differentiation from Corynebacterium Xerosis and Corynebacterium freneyi is described. Analysis of 16S rRNA gene sequences, rpoB sequences and the PCR profile of the 16S–23S spacer regions was not conclusive enough to differentiate strain C-138T from C. Xerosis and C. freneyi. However, according to DNA–DNA hybridization data, strain C-138T constitutes a member of a distinct novel species. It can be differentiated from strains of C. Xerosis and C. freneyi by colony morphology, the absence of α-glucosidase and some biochemical characteristics such as glucose fermentation at 42 °C and carbon assimilation substrates. The name Corynebacterium hansenii sp. nov. is proposed for this novel species; the type strain is C-138T (=CIP 108444T=CCUG 53252T).
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Corynebacterium hansenii sp. nov., an alpha-glucosidase-negative bacterium related to Corynebacterium Xerosis.
International journal of systematic and evolutionary microbiology, 2007Co-Authors: François N.r. Renaud, Philippe Riegel, Dominique Aubel, Alain Le Coustumier, Nathalie Wilhem, Claude Bollet, Jean FreneyAbstract:A novel strain, C-138(T), belonging to the genus Corynebacterium was isolated from a severe thigh liposarcoma infection and its differentiation from Corynebacterium Xerosis and Corynebacterium freneyi is described. Analysis of 16S rRNA gene sequences, rpoB sequences and the PCR profile of the 16S-23S spacer regions was not conclusive enough to differentiate strain C-138(T) from C. Xerosis and C. freneyi. However, according to DNA-DNA hybridization data, strain C-138(T) constitutes a member of a distinct novel species. It can be differentiated from strains of C. Xerosis and C. freneyi by colony morphology, the absence of alpha-glucosidase and some biochemical characteristics such as glucose fermentation at 42 degrees C and carbon assimilation substrates. The name Corynebacterium hansenii sp. nov. is proposed for this novel species; the type strain is C-138(T) (=CIP 108444(T)=CCUG 53252(T)).
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Corynebacterium freneyi sp. nov., alpha-glucosidase-positive strains related to Corynebacterium Xerosis.
International Journal of Systematic and Evolutionary Microbiology, 2001Co-Authors: François Renaud, Philippe Riegel, Dominique Aubel, Hélène Meugnier, Claude BolletAbstract:Three coryneform strains from clinical specimens were studied. They belonged to the genus Corynebacterium, since they had type IV cell walls containing corynemycolic acids. They had phenotypic characteristics that included alpha-glucosidase, pyrazinamidase and alkaline phosphatase activities and fermentation of glucose, ribose, maltose and sucrose. These are the characteristics of Corynebacterium Xerosis. Since this species is very rare in human pathology, the strains were studied in more detail by comparing the 16S-23S intergenic spacers, rDNA sequences and levels of DNA similarity of these three strains and those of the reference strains C. Xerosis ATCC 373T and Corynebacterium amycolatum CIP 103452T. According to DNA-DNA hybridization data, the three novel strains are members of the same species (level of DNA similarity >72%). Phylogenetic analysis revealed that these strains are closely related to C. Xerosis and C. amycolatum, but DNA-relatedness experiments showed clearly that they constitute a distinct new species, with levels of DNA relatedness of less than 23% to C. Xerosis ATCC 373T and less than 5% to C. amycolatum CIP 103452T. Two other alpha-glucosidase-positive strains presenting the same biochemical characteristics were included in the study and proved to be C. amycolatum. This new species can be differentiated from C. Xerosis and C. amycolatum strains by carbon source utilization, intergenic spacer region length profiles and some biochemical characteristics such as glucose fermentation at 42 degrees C and growth at 20 degrees C. The name Corynebacterium freneyi sp. nov. is proposed with the type strain ISPB 6695110T (= CIP 106767T = DSM 44506T).
Jean Freney - One of the best experts on this subject based on the ideXlab platform.
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Corynebacterium hansenii sp. nov., an α-glucosidase-negative bacterium related to Corynebacterium Xerosis
International Journal of Systematic and Evolutionary Microbiology, 2007Co-Authors: François N.r. Renaud, Philippe Riegel, Dominique Aubel, Alain Le Coustumier, Nathalie Wilhem, Claude Bollet, Jean FreneyAbstract:A novel strain, C-138T, belonging to the genus Corynebacterium was isolated from a severe thigh liposarcoma infection and its differentiation from Corynebacterium Xerosis and Corynebacterium freneyi is described. Analysis of 16S rRNA gene sequences, rpoB sequences and the PCR profile of the 16S–23S spacer regions was not conclusive enough to differentiate strain C-138T from C. Xerosis and C. freneyi. However, according to DNA–DNA hybridization data, strain C-138T constitutes a member of a distinct novel species. It can be differentiated from strains of C. Xerosis and C. freneyi by colony morphology, the absence of α-glucosidase and some biochemical characteristics such as glucose fermentation at 42 °C and carbon assimilation substrates. The name Corynebacterium hansenii sp. nov. is proposed for this novel species; the type strain is C-138T (=CIP 108444T=CCUG 53252T).
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Corynebacterium hansenii sp. nov., an alpha-glucosidase-negative bacterium related to Corynebacterium Xerosis.
International journal of systematic and evolutionary microbiology, 2007Co-Authors: François N.r. Renaud, Philippe Riegel, Dominique Aubel, Alain Le Coustumier, Nathalie Wilhem, Claude Bollet, Jean FreneyAbstract:A novel strain, C-138(T), belonging to the genus Corynebacterium was isolated from a severe thigh liposarcoma infection and its differentiation from Corynebacterium Xerosis and Corynebacterium freneyi is described. Analysis of 16S rRNA gene sequences, rpoB sequences and the PCR profile of the 16S-23S spacer regions was not conclusive enough to differentiate strain C-138(T) from C. Xerosis and C. freneyi. However, according to DNA-DNA hybridization data, strain C-138(T) constitutes a member of a distinct novel species. It can be differentiated from strains of C. Xerosis and C. freneyi by colony morphology, the absence of alpha-glucosidase and some biochemical characteristics such as glucose fermentation at 42 degrees C and carbon assimilation substrates. The name Corynebacterium hansenii sp. nov. is proposed for this novel species; the type strain is C-138(T) (=CIP 108444(T)=CCUG 53252(T)).
Pomposo Fernández Rosas - One of the best experts on this subject based on the ideXlab platform.
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Identification and molecular characterization of Corynebacterium Xerosis isolated from a sheep cutaneous abscess: first case report in Mexico
BMC research notes, 2016Co-Authors: Fernando Hernández-león, Jorge Acosta-dibarrat, Juan Carlos Vázquez-chagoyán, Pomposo Fernández Rosas, Roberto Montes De Oca-jiménezAbstract:Background Corynebacterium Xerosis is a commensal organism found in skin and mucous membranes of humans. It is considered an unusual pathogen, and it is rarely found in human and animal clinical samples. Here we describe the isolation of C. Xerosis from a 4-months-old Pelifolk lamb located in Tesistan, central western Mexico. This microorganism should be considered for differential diagnosis in cutaneous abscessed lesions in sheep, as it represents a zoonotic risk factor for human infection in sheep farms.
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Identification and molecular characterization of Corynebacterium Xerosis isolated from a sheep cutaneous abscess: first case report in Mexico
BMC Research Notes, 2016Co-Authors: Fernando Hernández-león, Jorge Acosta-dibarrat, Juan Carlos Vázquez-chagoyán, Pomposo Fernández Rosas, Roberto Montes De Oca-jiménezAbstract:Background Corynebacterium Xerosis is a commensal organism found in skin and mucous membranes of humans. It is considered an unusual pathogen, and it is rarely found in human and animal clinical samples. Here we describe the isolation of C. Xerosis from a 4-months-old Pelifolk lamb located in Tesistán, central western Mexico. This microorganism should be considered for differential diagnosis in cutaneous abscessed lesions in sheep, as it represents a zoonotic risk factor for human infection in sheep farms. Case presentation The animal exhibited a hard-consistency, 5 cm diameter abscess, without drainage, in the neck. The presumptive clinical diagnosis was caseous lymphadenitis, caused by Corynebacterium pseudotuberculosis . Samples were obtained by puncture and cultured in 8 % sheep blood agar under microaerophilic conditions. Colonies were non-haemolytic, brown-yellowish and showed microscopic and biochemical features similar to C. pseudotuberculosis , except for the urea test. A multiplex-PCR for the amplification of partial sequences of the pld , rpoB and intergenic fragment from 16S to 23S genes suggested that isolate could be C. Xerosis, which was later confirmed by sequencing analysis of the rpoB gene. Conclusions This study shows for the first time isolation and molecular characterization of C. Xerosis from a clinical sample of an ovine cutaneous abscess in Mexico. This finding highlights the need for differential diagnosis of this pathogen in ovine skin abscesses, as well as epidemiological and control studies of this pathogen in sheep farms.
