The Experts below are selected from a list of 150 Experts worldwide ranked by ideXlab platform
Jinhong Guo - One of the best experts on this subject based on the ideXlab platform.
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CMOS-Compatible Silicon-Nanowire-Based Coulter Counter for Cell Enumeration
IEEE Transactions on Biomedical Engineering, 2016Co-Authors: Yu Chen, Jinhong Guo, Hamidullah Muhammad, Yuejun Kang, Sunil K. AryAbstract:A silicon-nanowire-based Coulter Counter has been designed and fabricated for particle/cell enumeration. The silicon nanowire was fabricated in a fully complementary metal-oxide-semiconductor (CMOS)-compatible process and used as a field effect transistor (FET) device. The Coulter Counter device worked on the principle of potential change detection introduced by the passing of microparticles/cells through a sensing channel. Device uniformity was confirmed by scanning electron microscopy and transmission electron microscopy. Current-voltage measurement showed the high sensitivity of the nanowire FET device to the surface potential change. The results revealed that the silicon-nanowire-based Coulter Counter can differentiate polystyrene beads with diameters of 8 and 15 μm. Michigan Cancer Foundation-7 (MCF-7) cells have been successfully counted to validate the device. A fully CMOS-compatible fabrication process can help the device integration and facilitate the development of sensor arrays for high throughput application. With appropriate sample preparation steps, it is also possible to expand the work to applications such as rare-cells detection.
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portable Coulter Counter with vertical through holes for high throughput applications
Sensors and Actuators B-chemical, 2015Co-Authors: Yu Chen, Jinhong Guo, Yuejun Kang, Seongjin Kim, Jaya P Kausalya, Alicia Ghia Min Ong, Walter Hunziker, Jaehoon ChungAbstract:Abstract A high-throughput Coulter Counter for point-of-care (POC) applications has been developed, which measures the concentration and size distribution of cells in suspension at a single cell resolution. Vertical channels (silicon-based through-holes), instead of conventional planar ones, were employed to achieve a higher density of sensing channels for a high-throughput operation. The crosstalk from the multiple sensing channels was minimized by potentiometric measurement based on a three-electrode configuration, decoupling the neighboring channels electrically. The through-hole array and potentiometric sensing increased throughput up to 8000 cells/s in the prototype (designed with four channels). To secure the sensitivity over a wide range of cell sizes in a limited setting (a portable device powered by a battery power,
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3D numerical simulation of a Coulter Counter array with analysis of electrokinetic forces
Electrophoresis, 2012Co-Authors: Jinhong Guo, Tze Sian Pui, Abdur Rub Abdur Rahman, Yuejun KangAbstract:Coulter Counters have played an important role in biological cell assays since their introduction decades ago. Several types of high throughput micro-Coulter Counters based on lab-on-chip devices have been commercialized recently. In this paper, we propose a highly integrated micro-Coulter Counter array working under low DC voltage. The real-time electrical current change, including the pulse amplitude and width, of the micro-Coulter Counter with novel structure is systematically investigated numerically. The major types of forces exerted on the particle in the micro-Coulter Counter, including hydrodynamic force and electrokinetic force are quantitatively analyzed. The simulation in this study shows the pulse profile, such as width and amplitude, is affected by both particle size and the flow condition. The special cases of multiple particle aggregation and cross-talk between neighboring channels are also considered for their effects on the electric current pulses. This simulation provides critical insight and guidance for developing next new generations of micro-Coulter Counter.
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Numerical Investigation of the Performance of Coulter Counter with Novel Structure
2012Co-Authors: Jinhong Guo, Tze Sian Pui, Abdur Rub, Abdur Rahman, Yuejun KangAbstract: Abstract—Coulter Counters are instruments designed to count particles of various shapes and sizes. A significant limitation of the contemporary Coulter device is high throughput. In this article, a novel Coulter structure is proposed and the change of resistance due to particle translocation is studied numerically. The simulation results show the resistance change affected by the cell size as well as the cell location. The cases when cells stick together and non- stick to translocate in the channel are also analyzed numerically. A Coulter device with multiple sensing units is modeled and simulated to investigate the effect of cross-talk between sensing channels. The studies provide useful instructions for the design of solid-state vertical micro Coulter Counter for high throughput particles counting and scaling up for a Counter array.
Yuejun Kang - One of the best experts on this subject based on the ideXlab platform.
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CMOS-Compatible Silicon-Nanowire-Based Coulter Counter for Cell Enumeration
IEEE Transactions on Biomedical Engineering, 2016Co-Authors: Yu Chen, Jinhong Guo, Hamidullah Muhammad, Yuejun Kang, Sunil K. AryAbstract:A silicon-nanowire-based Coulter Counter has been designed and fabricated for particle/cell enumeration. The silicon nanowire was fabricated in a fully complementary metal-oxide-semiconductor (CMOS)-compatible process and used as a field effect transistor (FET) device. The Coulter Counter device worked on the principle of potential change detection introduced by the passing of microparticles/cells through a sensing channel. Device uniformity was confirmed by scanning electron microscopy and transmission electron microscopy. Current-voltage measurement showed the high sensitivity of the nanowire FET device to the surface potential change. The results revealed that the silicon-nanowire-based Coulter Counter can differentiate polystyrene beads with diameters of 8 and 15 μm. Michigan Cancer Foundation-7 (MCF-7) cells have been successfully counted to validate the device. A fully CMOS-compatible fabrication process can help the device integration and facilitate the development of sensor arrays for high throughput application. With appropriate sample preparation steps, it is also possible to expand the work to applications such as rare-cells detection.
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portable Coulter Counter with vertical through holes for high throughput applications
Sensors and Actuators B-chemical, 2015Co-Authors: Yu Chen, Jinhong Guo, Yuejun Kang, Seongjin Kim, Jaya P Kausalya, Alicia Ghia Min Ong, Walter Hunziker, Jaehoon ChungAbstract:Abstract A high-throughput Coulter Counter for point-of-care (POC) applications has been developed, which measures the concentration and size distribution of cells in suspension at a single cell resolution. Vertical channels (silicon-based through-holes), instead of conventional planar ones, were employed to achieve a higher density of sensing channels for a high-throughput operation. The crosstalk from the multiple sensing channels was minimized by potentiometric measurement based on a three-electrode configuration, decoupling the neighboring channels electrically. The through-hole array and potentiometric sensing increased throughput up to 8000 cells/s in the prototype (designed with four channels). To secure the sensitivity over a wide range of cell sizes in a limited setting (a portable device powered by a battery power,
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3D numerical simulation of a Coulter Counter array with analysis of electrokinetic forces
Electrophoresis, 2012Co-Authors: Jinhong Guo, Tze Sian Pui, Abdur Rub Abdur Rahman, Yuejun KangAbstract:Coulter Counters have played an important role in biological cell assays since their introduction decades ago. Several types of high throughput micro-Coulter Counters based on lab-on-chip devices have been commercialized recently. In this paper, we propose a highly integrated micro-Coulter Counter array working under low DC voltage. The real-time electrical current change, including the pulse amplitude and width, of the micro-Coulter Counter with novel structure is systematically investigated numerically. The major types of forces exerted on the particle in the micro-Coulter Counter, including hydrodynamic force and electrokinetic force are quantitatively analyzed. The simulation in this study shows the pulse profile, such as width and amplitude, is affected by both particle size and the flow condition. The special cases of multiple particle aggregation and cross-talk between neighboring channels are also considered for their effects on the electric current pulses. This simulation provides critical insight and guidance for developing next new generations of micro-Coulter Counter.
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Numerical Investigation of the Performance of Coulter Counter with Novel Structure
2012Co-Authors: Jinhong Guo, Tze Sian Pui, Abdur Rub, Abdur Rahman, Yuejun KangAbstract: Abstract—Coulter Counters are instruments designed to count particles of various shapes and sizes. A significant limitation of the contemporary Coulter device is high throughput. In this article, a novel Coulter structure is proposed and the change of resistance due to particle translocation is studied numerically. The simulation results show the resistance change affected by the cell size as well as the cell location. The cases when cells stick together and non- stick to translocate in the channel are also analyzed numerically. A Coulter device with multiple sensing units is modeled and simulated to investigate the effect of cross-talk between sensing channels. The studies provide useful instructions for the design of solid-state vertical micro Coulter Counter for high throughput particles counting and scaling up for a Counter array.
I Jovanovic - One of the best experts on this subject based on the ideXlab platform.
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On the applicability of the Coulter Counter to the cavitation nuclei size distribution analysis
International shipbuilding progress, 1993Co-Authors: A Dzubur, B Bajic, I JovanovicAbstract:The use of the Coulter-Counter method in analysing the size distribution of cavitation nuclei and bubbles in a cavitation tunnel is discussed. Relevant physical details are modelled and tested, and an experimental system is carefully designed. Even when extensively modified, the Coulter-Counter method poses serious difficulties not proving useful in experiments of this kind.
J Nygård - One of the best experts on this subject based on the ideXlab platform.
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Interference by cryoglobulins with white blood cell measurements on Coulter Counter.
Scandinavian journal of clinical and laboratory investigation, 1991Co-Authors: A Bremmelgaard, J NygårdAbstract:By using the Coulter Counter S-Plus IV erroneous measurements of leukocytes, platelets and red cells were experienced in blood from two patients with cryoglobulinaemia. Leukocyte levels were estimated too high owing to contamination of the leukocyte counting chamber by unlysed erythrocytes. One of the patients had leukopenia, but the Coulter Counter reported white blood cell counts within normal limits. Rewarming of the sample to 37 degrees C eliminated the problem.
Simon Mendez - One of the best experts on this subject based on the ideXlab platform.
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Numerical simulation of deformable particles in a Coulter Counter.
International journal for numerical methods in biomedical engineering, 2019Co-Authors: Pierre Taraconat, Jean‐philippe Gineys, Damien Isebe, Franck Nicoud, Simon MendezAbstract:In Coulter Counters, cells counting and volumetry are achieved by monitoring their electrical print when they flow through a sensing zone. However, the volume measurement may be impaired by the cell dynamics, which may be difficult to control. In this paper, numerical simulations of the dynamics and electrical signature of red blood cells in a Coulter Counter are presented, accounting for the deformability of the cells. In particular, a specific numerical pipeline is developed to overcome the challenge of the multi-scale nature of the problem. It consists in segmenting the whole computation of the cell dynamics and electrical response in a series of dedicated computations, with a saving of one order of magnitude in computational time. This numerical pipeline is used with rigid spheres and deformable red blood cells in an industrial Coulter Counter geometry, and compared with experimental measurements. The simulations not only reproduce electrical signatures typical of those measured experimentally, but also allow an analysis of the electrical signature in terms of the heterogeneity of the electrical field and dynamics of the particles in the measurement zone. This study provides a methodology for computing the sizing of rigid or deformable particles by Coulter Counters, opening the way to a better understanding of cells signatures in such devices.
