The Experts below are selected from a list of 423 Experts worldwide ranked by ideXlab platform
Kemp B. - One of the best experts on this subject based on the ideXlab platform.
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Meningococcal Disease Diagnostic Criteria In Greater Metropolitan Campinas, São Paulo State, Brazil [critério Diagnóstico Da Doença Meningocócica Na Região Metropolitana De Campinas, São Paulo, Brasil.]
2015Co-Authors: Donalisio M.r., Rocha M.m., Ramalheira R.m., Kemp B.Abstract:The aim of this article is to evaluate confirmatory criteria: culture, latex agglutination, Counter Immunoelectrophoresis, microscopic examination, and clinical/epidemiological criteria for cases of meningococcal disease reported in Greater Metropolitan Campinas, São Paulo State, Brazil, from 1993 to 2002 (568 cases). The following variables were also studied: clinical features, gender, age, city, hospital, case fatality, seasonality, and Neisseria meningitidis serogroup. Culture as a confirmatory criterion was the dependent variable in univariate analysis. The mean proportion of confirmatory criterion by culture was 68.7%. Clinical features of meningococcal disease -- meningitis without septicemia (OR = 2.87; CI: 1.89-4.38) and septicemia without meningitis (OR = 0.26; CI: 0.17-0.45) -- were associated with confirmation by culture. Case fatality rates were different among all diagnostic criteria. More attention should be given to etiological diagnostic confirmation in more severe cases. Diagnostic methods such as PCR may improve etiological confirmation of meningococcal disease in cases with negative cultures
Kemp Brigina - One of the best experts on this subject based on the ideXlab platform.
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[meningococcal Disease Diagnostic Criteria In Greater Metropolitan Campinas, São Paulo State, Brazil].
2015Co-Authors: Donalisio, Maria Rita, Rocha, Marilu Mendes Moscardini, Ramalheira, Raquel Maria Ferreira, Kemp BriginaAbstract:The aim of this article is to evaluate confirmatory criteria: culture, latex agglutination, Counter Immunoelectrophoresis, microscopic examination, and clinical/epidemiological criteria for cases of meningococcal disease reported in Greater Metropolitan Campinas, São Paulo State, Brazil, from 1993 to 2002 (568 cases). The following variables were also studied: clinical features, gender, age, city, hospital, case fatality, seasonality, and Neisseria meningitidis serogroup. Culture as a confirmatory criterion was the dependent variable in univariate analysis. The mean proportion of confirmatory criterion by culture was 68.7%. Clinical features of meningococcal disease -- meningitis without septicemia (OR = 2.87; CI: 1.89-4.38) and septicemia without meningitis (OR = 0.26; CI: 0.17-0.45) -- were associated with confirmation by culture. Case fatality rates were different among all diagnostic criteria. More attention should be given to etiological diagnostic confirmation in more severe cases. Diagnostic methods such as PCR may improve etiological confirmation of meningococcal disease in cases with negative cultures
Iftikhar Hussain And M. Ashfaque - One of the best experts on this subject based on the ideXlab platform.
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SEROLOGICAL STUDY OF PESTE-DES-PETITS RUMINANTS (PPR) USING Counter Immunoelectrophoresis IN FAISALABAD
University of Agriculture Faisalabad, 2000Co-Authors: Touseef M. Tahir, Rehan Ahmad1, Iftikhar Hussain And M. AshfaqueAbstract:Antibodies against peste-des-petits ruminants (PPR) in sheep and goats were detected using Counter Immunoelectrophoresis. Out of 208 sheep and 209 goats serum samples, 27.88 and 31.10 per cent, respectively were positive for the presence of antibodies against PPR. Sex, age and breed wise results were also recorded. The results indicated that PPR is widely distributed in sheep and goats
X Bossuyt - One of the best experts on this subject based on the ideXlab platform.
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Evaluation of a Dot-Blot Method for Identification of Antibodies against Extractable Nuclear Antigens and Anti-Cytoplasmic Antibodies
Clinical Chemistry, 1999Co-Authors: Alex Mewis, Godelieve Mariën, Norbert Blanckaert, X BossuytAbstract:Antibodies against extractable nuclear antigens (ENAs) are useful diagnostic markers for various autoimmune diseases. Anti-SSA and anti-SSB antibodies are found in Sjogren Syndrome (SS), anti-Sm antibodies are found in systemic lupus erythematosus (SLE), anti-RNP antibodies are found in mixed connective tissue disease (MCTD), and anti-Scl-70 antibodies are found in scleroderma. Antibodies against cytoplasmic Jo-1 and M2 are helpful markers for the diagnosis of, respectively, polymyositis and primary biliary cirrhosis. The antibodies can be identified by either Counter Immunoelectrophoresis (CIE) or immunoblotting. These techniques, however, are time-consuming, technically demanding, and do not provide for fast turnaround times. Over the last few years, alternative …
L Krishna - One of the best experts on this subject based on the ideXlab platform.
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comparative evaluation of agar gel precipitation CounterImmunoelectrophoresis and passive haemagglutination tests for the diagnosis of dicrocoelium dendriticum infection in sheep and goats
Veterinary Parasitology, 1996Co-Authors: K P Jithendran, Jyotsna Vaid, L KrishnaAbstract:Abstract The sensitivity and specificity of the agar gel precipitation test (AGPT), Counter Immunoelectrophoresis (CIEP) and passive haemagglutination test (PHT) were evaluated for the diagnosis of Dicrocoelium dendriticum infection in naturally infected sheep and goats. Two hundred and forty five sheep and goat sera samples were tested using phosphate buffered saline, pH 7.2 extracted adult fluke antigen. CIEP detected 69.8% of the infected animals and was found to be the most sensitive, followed by PHT which detected 50.0% of the infected sheep and goats. AGPT was found to be the least sensitive, detecting only 23.8% of the infected animals. The specificity of AGPT, CIEP and PHT were 93.3%, 84.0% and 93.3%, respectively. CIEP was found to be the most sensitive, specific and rapid test for the seroepidemiological survey of dicrocoeliosis in sheep and goats.