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Zeng Lin - One of the best experts on this subject based on the ideXlab platform.

  • Comparison of Gene Expression Levels of TYR、TYRP1 Gene in Cricetulus barabensis and the Albino mutant
    Chinese Journal of Comparative Medicine, 2012
    Co-Authors: Zeng Lin
    Abstract:

    Objective To explore the relationship between gene expression of Cricetulus barabensis TYR gene family and Cricetulus barabensis coat color.Methods The relative expression quantity of TYR,TYRP1 in Cricetulus barabensis of different colors was analyzed by using real time quantitative PCR in this research.Results The relative expression quantity of TYR,TYRP1 in Cricetulus barabensis respectively were 2.5,5.3 times than that in the Albino mutant,all results were corrected by the household gene.Conclusion The findings indicated that gene expression level of TYR gene family in Cricetulus barabensis were higher than that in the Albino mutant,and the gene expression level of TYR gene family were related with phenotype of Cricetulus barabensis coat color.

  • comparison of gene expression levels of tyr tyrp1 gene in Cricetulus barabensis and the albino mutant
    Chinese Journal of Comparative Medicine, 2012
    Co-Authors: Zeng Lin
    Abstract:

    Objective To explore the relationship between gene expression of Cricetulus barabensis TYR gene family and Cricetulus barabensis coat color.Methods The relative expression quantity of TYR,TYRP1 in Cricetulus barabensis of different colors was analyzed by using real time quantitative PCR in this research.Results The relative expression quantity of TYR,TYRP1 in Cricetulus barabensis respectively were 2.5,5.3 times than that in the Albino mutant,all results were corrected by the household gene.Conclusion The findings indicated that gene expression level of TYR gene family in Cricetulus barabensis were higher than that in the Albino mutant,and the gene expression level of TYR gene family were related with phenotype of Cricetulus barabensis coat color.

  • Cloning and sequence analysis of tyrosinase gene in Cricetulus barabensis and albino mutant Cricetulus barabensis
    2012
    Co-Authors: Zeng Lin
    Abstract:

    Objective In order to reveal the molecular mechanism of the albino mutant Cricetulus barabensis,the tyrosinase gene of Cricetulus barabensis and the albino mutant Cricetulus barabensis was cloned,sequenced,and comparatively analyzed.Methods The Tyrosinase cDNA was amplified from the total RNA of the skin of Cricetulus barabensis and its albino mutant Cricetulus barabensis by reverse transcriptase(RT-PCR).According to the conservative region of tyrosinase gene of mice and rats,three pairs of primers were designed to link three fragments to a long epitope by overlap extension.Then the three fragments were amplified and sequenced and finally spliced into the complete sequence.The mutation site was identified through DNA matching software.Results The tyrosinase genes of Cricetulus barabensis and the albino mutant Cricetulus barabensis were cloned and the mutation site was measured by DNA sequence analysis software.The results showed that they have the same coding region and there is no mutation.Conclusions The reason of the albino trait of the albino mutant Cricetulus barabensis is different from that of albino trait known in mice,not by the mutation of tyrosinase gene coding region,and reqires further investigation.

  • Histochemical Study on the Distribution of Melanocytes in Hair Folicle of the Cricetulus barabensis and the Albino Mutant
    Chinese Journal of Comparative Medicine, 2010
    Co-Authors: Zeng Lin
    Abstract:

    Objective To observe the distribution of melanocytes in skin of the Cricetulus barabensis and the albino mutant,and to explain the cytologic basis of the the albino mutant. Methods The skin of back were surgically separated from six Cricetulus barabensis and the albino mutant,paraffin sections were prepared conventionally. Dopa staining and dopa-toluidine blue staining were executed to obtain the visible information about melanocytes distribution. Results The results showed that the melanocytes were mainly distributed in the dermal papilla of the hair follicles,and the are was more significant in Cricetulus barabensis than the in albino mutant. Conclusion The tyrosinase activity of the Cricetulus barabensis is higher than the albino mutant.

Steffen Maak - One of the best experts on this subject based on the ideXlab platform.

  • molecular phylogeny of the cricetinae subfamily based on the mitochondrial cytochrome b and 12s rrna genes and the nuclear vwf gene
    Molecular Phylogenetics and Evolution, 2006
    Co-Authors: Karsten Neumann, Johan Michaux, V S Lebedev, Nuri Yigit, Ercument Colak, Natalia Ivanova, Andrey Poltoraus, A V Surov, Georgi Markov, Steffen Maak
    Abstract:

    Despite some popularity of hamsters as pets and laboratory animals there is no reliable phylogeny of the subfamily Cricetinae available so far. Contradicting views exist not only about the actual number of species but also concerning the validity of several genera. We used partial DNA sequences of two mitochondrial (cytochrome b, 12S rRNA) and one partial nuclear gene (von Willebrand Factor exon 28) to provide a first gene tree of the Cricetinae based on 15 taxa comprising six genera. According to our data, Palaearctic hamsters fall into three distinct phylogenetic groups: Phodopus, Mesocricetus, and Cricetus-related species which evolved during the late Miocene about 7–12 MY ago. Surprisingly, the genus Phodopus, which was previously thought to have appeared during the Pleistocene, forms the oldest clade. The largest number of extant hamster genera is found in a group of Cricetus-related hamsters. The genus Cricetulus itself proved to be not truly monophyletic with Cricetulus migratorius appearing more closely related to Tscherskia, Cricetus, and AlloCricetulus. We propose to place the species within a new monotypic genus. Molecular clock calculations are not always in line with the dating of fossil records. DNA based divergence time estimates as well as taxonomic relationships demand a reevaluation of morphological characters previously used to identify fossils and extant hamsters.

Richard J. Hurley - One of the best experts on this subject based on the ideXlab platform.

Clifford E. Desch - One of the best experts on this subject based on the ideXlab platform.

Jing Cao - One of the best experts on this subject based on the ideXlab platform.