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Evidente A. - One of the best experts on this subject based on the ideXlab platform.
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Gigantelline, gigantellinine and gigancrinine, cherylline- and crinine-type alkaloids isolated from Crinum jagus with anti-acetylcholinesterase activity
'Elsevier BV', 2020Co-Authors: Ka S., Masi M., Merindol N., Di Lecce R., Plourde M. B., Seck M., Gorecki M., Pescitelli G., Evidente A.Abstract:Three undescribed Amarylidaceae alkaloids, named gigantelline, gigantellinine and gigancrinine, were isolated from Crinum jagus (syn. = Crinum giganteum) collected in Senegal, together with the already known sanguinine, cherylline, lycorine, crinine, flexinine and the isoquinolinone derivative hippadine. Gigantelline, gigantellinine and gigancrinine were characterized as 4-(6,7-dimethoxy-2-methyl-1,2,3,4-tetrahydro-isoquinolin-4-yl)-phenol, its 7-O-demethyl-5ꞌ-hydroxy-4ꞌ-methoxy derivative and 5,6a,7,7a,8a,9-hexahydro-6,9a-ethano[1,3]dioxolo[4,5-j]oxireno[2,3-b]phenanthridin-9-ol, respectively, by using spectroscopic (1D and 2D 1H and 13C NMR and HRESIMS) and chemical methods. Their relative configuration was assigned by NOESY NMR spectra and NMR calculations, while the absolute configuration was assigned using electronic circular dichroism (ECD) experiments and calculations. Sanguinine, cherylline, crinine, flexinine, and the isoquinolinone hippadine, were isolated for the first time from C. jagus. Cherylline, gigantellinine, crinine, flexinine and sanguinine inhibited the activity of AChE in a dose-dependent manner, and inhibition by sanguinine was remarkably effective (IC50 = 1.83 ± 0.01 μM). Cherylline and hippadine showed weak cytotoxicity at 100 μM
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A Brief Up-to-Date Overview of Amaryllidaceae Alkaloids: Phytochemical Studies of Narcissus tazetta subsp. tazetta L., Collected in Turkey
'SAGE Publications', 2019Co-Authors: Karakoyun C., Masi M., Cimmino A., Somer N.u., Kornienko A., Evidente A.Abstract:A brief up-to-date overview on the isolation, and chemical and biological characterization of new and known alkaloids from different Amaryllidaceae species, including Brunsvigia, Crinum, Cyrtanthus, Narcissus, and Nerine genera, was reported. Furthermore, the isolation and chemical characterization of alkaloids extracted from bulbs of Narcissus tazetta subsp. tazetta L. collected from Muğla, Turkey were described. © The Author(s) 2019.The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This study was supported by the academic grant from the Department of Chemical Sciences of University of Naples Federico II
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Alkaloids isolated from indigenous South African Amaryllidaceae: Crinum buphanoides (Welw. ex Baker), Crinum graminicola (I. Verd.), Cyrtanthus mackenii (Hook. f) and Brunsvigia grandiflora (Lindl)
'Elsevier BV', 2018Co-Authors: Masi M., Mubaiwa B., Cimmino A., Van Otterlo W. A. L., Green I. R., Mabank T., Karakoyun Ç., Evidente A.Abstract:Screening for alkaloids produced in four indigenous South African Amaryllidaceae plants, namely, Crinum buphanoides Welw. ex Baker, Crinum graminicola I. Verd., Cyrtanthus mackenii Hook. f and Brunsvigia grandiflora Lindl, leads to the isolation of lycorine as the one metabolite produced by all the species studied. C. graminicola produced lycorine in the best yield (2.1 g/kg). Moreover, 1-O-acetyl- and 2-O-acetyl-lycorine, pratorimine, hippadine and tazettine were isolated from C. buphanoides. Haemanthidine, haemanthamine and criwelline were isolated fromC. graminicola,while tazettine and 11-hydroxyvittatinewere produced by C. mackenii. Finally, crinamine and 11-hydroxyvittatine were isolated from B. grandiflora. This is the very first report on the isolation of these alkaloids from the four South African Amaryllidaceae. Furthermore, some interesting chemosystematic evaluations are reported
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Alkaloids isolated from indigenous South African Amaryllidaceae: Crinum buphanoides (Welw. ex Baker), Crinum graminicola (I. Verd.), Cyrtanthus mackenii (Hook. f) and Brunsvigia grandiflora (Lindl)
'Elsevier BV', 2018Co-Authors: Masi M., Mubaiwa B., Cimmino A., Van Otterlo W. A. L., Green I. R., Mabank T., Karakoyun C., Evidente A.Abstract:WOS: 000445521800024Screening for alkaloids produced in four indigenous South African Amaryllidaceae plants, namely, Crinum buphanoides Welw. ex Baker, Crinum graminicola I. Verd., Cyrtanthus mackenii Hook. f and Brunsvigia grandiflora Lindl, leads to the isolation of lycorine as the one metabolite produced by all the species studied. C. graminicola produced lycorine in the best yield (2.1 g/kg). Moreover, 1-O-acetyl-and 2-O-acetyl-lycorine, pratorimine, hippadine and tazettine were isolated from C. buphanoides. Haemanthidine, haemanthamine and criwelline were isolated from C. graminicola, while tazettine and 11-hydroxyvittatine were produced by C. mackenii. Finally, crinamine and 11-hydroxyvittatine were isolated from B. grandiflora. This is the very first report on the isolation of these alkaloids from the four South African Amaryllidaceae. Furthermore, some interesting chemosystematic evaluations are reported. (c) 2018 SAAB. Published by Elsevier B.V. All rights reserved.South-Africa Ministry of Foreign Affairs Area: South Africa, Medicine and Health [ZA14MO06]; UniNA; Compagnia di San PaoloCompagnia di San Paolo [E62F16001250003]; National Research Foundation of South AfricaNational Research Foundation - South Africa [113322]The work was supported in part by grants from a bilateral project between the Italian and South-Africa Ministry of Foreign Affairs Area: South Africa, Medicine and Health Project number: ZA14MO06, titled: Bioactive metabolites from South-African plants with anticancer activity and in part by the Programme STAR 2017, financially supported by UniNA and Compagnia di San Paolo grant number E62F16001250003. WvO and IRG thank the South African National Research Foundation (NRF) (Incentive and CPRR funding), and Stellenbosch University (Faculty and Departmental support). WvO and IRG acknowledge this work is based in part by support from the National Research Foundation of South Africa (Grant Number 113322). A NEMBA application is being processed. The authors thank Mr. Pierfrancesco Motti and Ms. Nadia Serino for their contributions to this work and thank Dr. Martin Smit (Curator - Stellenbosch University Botanical Garden) for facilitation of live plant specimens
Masi M. - One of the best experts on this subject based on the ideXlab platform.
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Gigantelline, gigantellinine and gigancrinine, cherylline- and crinine-type alkaloids isolated from Crinum jagus with anti-acetylcholinesterase activity
'Elsevier BV', 2020Co-Authors: Ka S., Masi M., Merindol N., Di Lecce R., Plourde M. B., Seck M., Gorecki M., Pescitelli G., Evidente A.Abstract:Three undescribed Amarylidaceae alkaloids, named gigantelline, gigantellinine and gigancrinine, were isolated from Crinum jagus (syn. = Crinum giganteum) collected in Senegal, together with the already known sanguinine, cherylline, lycorine, crinine, flexinine and the isoquinolinone derivative hippadine. Gigantelline, gigantellinine and gigancrinine were characterized as 4-(6,7-dimethoxy-2-methyl-1,2,3,4-tetrahydro-isoquinolin-4-yl)-phenol, its 7-O-demethyl-5ꞌ-hydroxy-4ꞌ-methoxy derivative and 5,6a,7,7a,8a,9-hexahydro-6,9a-ethano[1,3]dioxolo[4,5-j]oxireno[2,3-b]phenanthridin-9-ol, respectively, by using spectroscopic (1D and 2D 1H and 13C NMR and HRESIMS) and chemical methods. Their relative configuration was assigned by NOESY NMR spectra and NMR calculations, while the absolute configuration was assigned using electronic circular dichroism (ECD) experiments and calculations. Sanguinine, cherylline, crinine, flexinine, and the isoquinolinone hippadine, were isolated for the first time from C. jagus. Cherylline, gigantellinine, crinine, flexinine and sanguinine inhibited the activity of AChE in a dose-dependent manner, and inhibition by sanguinine was remarkably effective (IC50 = 1.83 ± 0.01 μM). Cherylline and hippadine showed weak cytotoxicity at 100 μM
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A Brief Up-to-Date Overview of Amaryllidaceae Alkaloids: Phytochemical Studies of Narcissus tazetta subsp. tazetta L., Collected in Turkey
'SAGE Publications', 2019Co-Authors: Karakoyun C., Masi M., Cimmino A., Somer N.u., Kornienko A., Evidente A.Abstract:A brief up-to-date overview on the isolation, and chemical and biological characterization of new and known alkaloids from different Amaryllidaceae species, including Brunsvigia, Crinum, Cyrtanthus, Narcissus, and Nerine genera, was reported. Furthermore, the isolation and chemical characterization of alkaloids extracted from bulbs of Narcissus tazetta subsp. tazetta L. collected from Muğla, Turkey were described. © The Author(s) 2019.The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This study was supported by the academic grant from the Department of Chemical Sciences of University of Naples Federico II
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First isolation of acetovanillone and piceol from Crinum buphanoides and Crinum graminicola (I. Verd.) Amaryllidaceae
'Elsevier BV', 2018Co-Authors: Masi M., Mubaiwa B., Cimmino A., Van Otterlo W. A. L., Green I. R.Abstract:Screening of three native South African Amaryllidaceae bulbs, aimed at finding new metabolites for their promising biological activities, lead to the initial discovery of two interesting non-alkaloid compounds viz., acetovanillone 1 (also known as apocynin) and 4-hydroxyacetophenone 2 (also named piceol) isolated from Crinum buphonoides, while only the former was isolated from Crinum graminicola. This is the first time that acetovanillone 1 and piceol 2 were isolated from C. graminicola and C. buphanoides, respectively. Acetovanillone 1 was recently reported as a metabolite of Boophane disticha (L.f.), another South Africa Amaryllidacea species
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Alkaloids isolated from indigenous South African Amaryllidaceae: Crinum buphanoides (Welw. ex Baker), Crinum graminicola (I. Verd.), Cyrtanthus mackenii (Hook. f) and Brunsvigia grandiflora (Lindl)
'Elsevier BV', 2018Co-Authors: Masi M., Mubaiwa B., Cimmino A., Van Otterlo W. A. L., Green I. R., Mabank T., Karakoyun Ç., Evidente A.Abstract:Screening for alkaloids produced in four indigenous South African Amaryllidaceae plants, namely, Crinum buphanoides Welw. ex Baker, Crinum graminicola I. Verd., Cyrtanthus mackenii Hook. f and Brunsvigia grandiflora Lindl, leads to the isolation of lycorine as the one metabolite produced by all the species studied. C. graminicola produced lycorine in the best yield (2.1 g/kg). Moreover, 1-O-acetyl- and 2-O-acetyl-lycorine, pratorimine, hippadine and tazettine were isolated from C. buphanoides. Haemanthidine, haemanthamine and criwelline were isolated fromC. graminicola,while tazettine and 11-hydroxyvittatinewere produced by C. mackenii. Finally, crinamine and 11-hydroxyvittatine were isolated from B. grandiflora. This is the very first report on the isolation of these alkaloids from the four South African Amaryllidaceae. Furthermore, some interesting chemosystematic evaluations are reported
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Alkaloids isolated from indigenous South African Amaryllidaceae: Crinum buphanoides (Welw. ex Baker), Crinum graminicola (I. Verd.), Cyrtanthus mackenii (Hook. f) and Brunsvigia grandiflora (Lindl)
'Elsevier BV', 2018Co-Authors: Masi M., Mubaiwa B., Cimmino A., Van Otterlo W. A. L., Green I. R., Mabank T., Karakoyun C., Evidente A.Abstract:WOS: 000445521800024Screening for alkaloids produced in four indigenous South African Amaryllidaceae plants, namely, Crinum buphanoides Welw. ex Baker, Crinum graminicola I. Verd., Cyrtanthus mackenii Hook. f and Brunsvigia grandiflora Lindl, leads to the isolation of lycorine as the one metabolite produced by all the species studied. C. graminicola produced lycorine in the best yield (2.1 g/kg). Moreover, 1-O-acetyl-and 2-O-acetyl-lycorine, pratorimine, hippadine and tazettine were isolated from C. buphanoides. Haemanthidine, haemanthamine and criwelline were isolated from C. graminicola, while tazettine and 11-hydroxyvittatine were produced by C. mackenii. Finally, crinamine and 11-hydroxyvittatine were isolated from B. grandiflora. This is the very first report on the isolation of these alkaloids from the four South African Amaryllidaceae. Furthermore, some interesting chemosystematic evaluations are reported. (c) 2018 SAAB. Published by Elsevier B.V. All rights reserved.South-Africa Ministry of Foreign Affairs Area: South Africa, Medicine and Health [ZA14MO06]; UniNA; Compagnia di San PaoloCompagnia di San Paolo [E62F16001250003]; National Research Foundation of South AfricaNational Research Foundation - South Africa [113322]The work was supported in part by grants from a bilateral project between the Italian and South-Africa Ministry of Foreign Affairs Area: South Africa, Medicine and Health Project number: ZA14MO06, titled: Bioactive metabolites from South-African plants with anticancer activity and in part by the Programme STAR 2017, financially supported by UniNA and Compagnia di San Paolo grant number E62F16001250003. WvO and IRG thank the South African National Research Foundation (NRF) (Incentive and CPRR funding), and Stellenbosch University (Faculty and Departmental support). WvO and IRG acknowledge this work is based in part by support from the National Research Foundation of South Africa (Grant Number 113322). A NEMBA application is being processed. The authors thank Mr. Pierfrancesco Motti and Ms. Nadia Serino for their contributions to this work and thank Dr. Martin Smit (Curator - Stellenbosch University Botanical Garden) for facilitation of live plant specimens
Olaniyi Amos Fawole - One of the best experts on this subject based on the ideXlab platform.
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anti inflammatory anticholinesterase antioxidant and phytochemical properties of medicinal plants used for pain related ailments in south africa
Journal of Ethnopharmacology, 2010Co-Authors: Olaniyi Amos Fawole, J.f. Finnie, S O Amoo, A R Ndhlala, M E Light, J. Van StadenAbstract:ETHNOPHARMACOLOGICAL RELEVANCE: Extracts of seven South African medicinal plants used traditionally for the treatment of pain-related ailments were evaluated. AIMS OF THE STUDY: The study was aimed at evaluating medicinal and therapeutic potentials of the investigated traditional medicinal plants. Plant extracts were evaluated for anti-inflammatory activity and other pharmacological properties such as anticholinesterase and antioxidant activities. Phytochemical analysis of total phenolic contents, condensed tannins, gallotannins and flavonoids in the aqueous methanol extracts of the medicinal plants were also carried out. MATERIALS AND METHODS: The evaluation of anti-inflammatory activity of 50% methanol (50% MeOH), petroleum ether (PE), dichloromethane (DCM) and ethanol (EtOH) plant extracts was done against cyclooxygenase-1 and -2 (COX-1 and COX-2) enzymes. 50% MeOH, PE, DCM and EtOH extracts were tested for acetylcholinesterase (AChE) inhibition, while 50% MeOH extracts were tested for 2,2-diphenyl-1-picryl hydrazyl (DPPH) radical scavenging activity and ferric-reducing power in the antioxidant assays. Total phenolic compounds, condensed tannins, gallotannins and flavonoids were quantitatively determined using spectrophotometric methods. RESULTS: At the screening assay concentration (0.25 microg/microl), 13 extracts showed good COX-1 inhibitory activity (>50%), while good activity was observed in 15 extracts against COX-2 enzyme. All the extracts of Crinum moorei (bulbs) showed good inhibition against both COX-1 and COX-2 enzymes. Though not significantly different (P=0.05), the highest COX-1 percentage inhibition (100%) was shown by Aloe ferox leaf PE and Colocasia antiquorum tuber DCM extracts, while Colocasia antiquorum tuber PE extract exhibited the highest (92.7%) percentage inhibition against COX-2. Crinum moorei bulb DCM extract showed the lowest EC(50) value (2.9 microg/ml) in the AChE assay. In addition, good to moderate bioactivities were observed in some extracts of Aloe ferox (leaves), Crinum moorei (bulbs) and Pycnostachys reticulata (leaves) in all the assays. The presence and/or amounts of phenolic compounds varied with plant species. CONCLUSION: The results obtained in this study validate the use of the investigated medicinal plants in South African traditional medicine for pain-related ailments.
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anti inflammatory anticholinesterase antioxidant and phytochemical properties of medicinal plants used for pain related ailments in south africa
Journal of Ethnopharmacology, 2010Co-Authors: Olaniyi Amos Fawole, J.f. Finnie, S O Amoo, A R Ndhlala, M E Light, J. Van StadenAbstract:Abstract Ethnopharmacological relevance Extracts of seven South African medicinal plants used traditionally for the treatment of pain-related ailments were evaluated. Aims of the study The study was aimed at evaluating medicinal and therapeutic potentials of the investigated traditional medicinal plants. Plant extracts were evaluated for anti-inflammatory activity and other pharmacological properties such as anticholinesterase and antioxidant activities. Phytochemical analysis of total phenolic contents, condensed tannins, gallotannins and flavonoids in the aqueous methanol extracts of the medicinal plants were also carried out. Materials and methods The evaluation of anti-inflammatory activity of 50% methanol (50% MeOH), petroleum ether (PE), dichloromethane (DCM) and ethanol (EtOH) plant extracts was done against cyclooxygenase-1 and -2 (COX-1 and COX-2) enzymes. 50% MeOH, PE, DCM and EtOH extracts were tested for acetylcholinesterase (AChE) inhibition, while 50% MeOH extracts were tested for 2,2-diphenyl-1-picryl hydrazyl (DPPH) radical scavenging activity and ferric-reducing power in the antioxidant assays. Total phenolic compounds, condensed tannins, gallotannins and flavonoids were quantitatively determined using spectrophotometric methods. Results At the screening assay concentration (0.25 μg/μl), 13 extracts showed good COX-1 inhibitory activity (>50%), while good activity was observed in 15 extracts against COX-2 enzyme. All the extracts of Crinum moorei (bulbs) showed good inhibition against both COX-1 and COX-2 enzymes. Though not significantly different ( P = 0.05), the highest COX-1 percentage inhibition (100%) was shown by Aloe ferox leaf PE and Colocasia antiquorum tuber DCM extracts, while Colocasia antiquorum tuber PE extract exhibited the highest (92.7%) percentage inhibition against COX-2. Crinum moorei bulb DCM extract showed the lowest EC 50 value (2.9 μg/ml) in the AChE assay. In addition, good to moderate bioactivities were observed in some extracts of Aloe ferox (leaves), Crinum moorei (bulbs) and Pycnostachys reticulata (leaves) in all the assays. The presence and/or amounts of phenolic compounds varied with plant species. Conclusion The results obtained in this study validate the use of the investigated medicinal plants in South African traditional medicine for pain-related ailments.
Cimmino A. - One of the best experts on this subject based on the ideXlab platform.
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A Brief Up-to-Date Overview of Amaryllidaceae Alkaloids: Phytochemical Studies of Narcissus tazetta subsp. tazetta L., Collected in Turkey
'SAGE Publications', 2019Co-Authors: Karakoyun C., Masi M., Cimmino A., Somer N.u., Kornienko A., Evidente A.Abstract:A brief up-to-date overview on the isolation, and chemical and biological characterization of new and known alkaloids from different Amaryllidaceae species, including Brunsvigia, Crinum, Cyrtanthus, Narcissus, and Nerine genera, was reported. Furthermore, the isolation and chemical characterization of alkaloids extracted from bulbs of Narcissus tazetta subsp. tazetta L. collected from Muğla, Turkey were described. © The Author(s) 2019.The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This study was supported by the academic grant from the Department of Chemical Sciences of University of Naples Federico II
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First isolation of acetovanillone and piceol from Crinum buphanoides and Crinum graminicola (I. Verd.) Amaryllidaceae
'Elsevier BV', 2018Co-Authors: Masi M., Mubaiwa B., Cimmino A., Van Otterlo W. A. L., Green I. R.Abstract:Screening of three native South African Amaryllidaceae bulbs, aimed at finding new metabolites for their promising biological activities, lead to the initial discovery of two interesting non-alkaloid compounds viz., acetovanillone 1 (also known as apocynin) and 4-hydroxyacetophenone 2 (also named piceol) isolated from Crinum buphonoides, while only the former was isolated from Crinum graminicola. This is the first time that acetovanillone 1 and piceol 2 were isolated from C. graminicola and C. buphanoides, respectively. Acetovanillone 1 was recently reported as a metabolite of Boophane disticha (L.f.), another South Africa Amaryllidacea species
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Alkaloids isolated from indigenous South African Amaryllidaceae: Crinum buphanoides (Welw. ex Baker), Crinum graminicola (I. Verd.), Cyrtanthus mackenii (Hook. f) and Brunsvigia grandiflora (Lindl)
'Elsevier BV', 2018Co-Authors: Masi M., Mubaiwa B., Cimmino A., Van Otterlo W. A. L., Green I. R., Mabank T., Karakoyun Ç., Evidente A.Abstract:Screening for alkaloids produced in four indigenous South African Amaryllidaceae plants, namely, Crinum buphanoides Welw. ex Baker, Crinum graminicola I. Verd., Cyrtanthus mackenii Hook. f and Brunsvigia grandiflora Lindl, leads to the isolation of lycorine as the one metabolite produced by all the species studied. C. graminicola produced lycorine in the best yield (2.1 g/kg). Moreover, 1-O-acetyl- and 2-O-acetyl-lycorine, pratorimine, hippadine and tazettine were isolated from C. buphanoides. Haemanthidine, haemanthamine and criwelline were isolated fromC. graminicola,while tazettine and 11-hydroxyvittatinewere produced by C. mackenii. Finally, crinamine and 11-hydroxyvittatine were isolated from B. grandiflora. This is the very first report on the isolation of these alkaloids from the four South African Amaryllidaceae. Furthermore, some interesting chemosystematic evaluations are reported
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Alkaloids isolated from indigenous South African Amaryllidaceae: Crinum buphanoides (Welw. ex Baker), Crinum graminicola (I. Verd.), Cyrtanthus mackenii (Hook. f) and Brunsvigia grandiflora (Lindl)
'Elsevier BV', 2018Co-Authors: Masi M., Mubaiwa B., Cimmino A., Van Otterlo W. A. L., Green I. R., Mabank T., Karakoyun C., Evidente A.Abstract:WOS: 000445521800024Screening for alkaloids produced in four indigenous South African Amaryllidaceae plants, namely, Crinum buphanoides Welw. ex Baker, Crinum graminicola I. Verd., Cyrtanthus mackenii Hook. f and Brunsvigia grandiflora Lindl, leads to the isolation of lycorine as the one metabolite produced by all the species studied. C. graminicola produced lycorine in the best yield (2.1 g/kg). Moreover, 1-O-acetyl-and 2-O-acetyl-lycorine, pratorimine, hippadine and tazettine were isolated from C. buphanoides. Haemanthidine, haemanthamine and criwelline were isolated from C. graminicola, while tazettine and 11-hydroxyvittatine were produced by C. mackenii. Finally, crinamine and 11-hydroxyvittatine were isolated from B. grandiflora. This is the very first report on the isolation of these alkaloids from the four South African Amaryllidaceae. Furthermore, some interesting chemosystematic evaluations are reported. (c) 2018 SAAB. Published by Elsevier B.V. All rights reserved.South-Africa Ministry of Foreign Affairs Area: South Africa, Medicine and Health [ZA14MO06]; UniNA; Compagnia di San PaoloCompagnia di San Paolo [E62F16001250003]; National Research Foundation of South AfricaNational Research Foundation - South Africa [113322]The work was supported in part by grants from a bilateral project between the Italian and South-Africa Ministry of Foreign Affairs Area: South Africa, Medicine and Health Project number: ZA14MO06, titled: Bioactive metabolites from South-African plants with anticancer activity and in part by the Programme STAR 2017, financially supported by UniNA and Compagnia di San Paolo grant number E62F16001250003. WvO and IRG thank the South African National Research Foundation (NRF) (Incentive and CPRR funding), and Stellenbosch University (Faculty and Departmental support). WvO and IRG acknowledge this work is based in part by support from the National Research Foundation of South Africa (Grant Number 113322). A NEMBA application is being processed. The authors thank Mr. Pierfrancesco Motti and Ms. Nadia Serino for their contributions to this work and thank Dr. Martin Smit (Curator - Stellenbosch University Botanical Garden) for facilitation of live plant specimens
J. Van Staden - One of the best experts on this subject based on the ideXlab platform.
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anti inflammatory anticholinesterase antioxidant and phytochemical properties of medicinal plants used for pain related ailments in south africa
Journal of Ethnopharmacology, 2010Co-Authors: Olaniyi Amos Fawole, J.f. Finnie, S O Amoo, A R Ndhlala, M E Light, J. Van StadenAbstract:Abstract Ethnopharmacological relevance Extracts of seven South African medicinal plants used traditionally for the treatment of pain-related ailments were evaluated. Aims of the study The study was aimed at evaluating medicinal and therapeutic potentials of the investigated traditional medicinal plants. Plant extracts were evaluated for anti-inflammatory activity and other pharmacological properties such as anticholinesterase and antioxidant activities. Phytochemical analysis of total phenolic contents, condensed tannins, gallotannins and flavonoids in the aqueous methanol extracts of the medicinal plants were also carried out. Materials and methods The evaluation of anti-inflammatory activity of 50% methanol (50% MeOH), petroleum ether (PE), dichloromethane (DCM) and ethanol (EtOH) plant extracts was done against cyclooxygenase-1 and -2 (COX-1 and COX-2) enzymes. 50% MeOH, PE, DCM and EtOH extracts were tested for acetylcholinesterase (AChE) inhibition, while 50% MeOH extracts were tested for 2,2-diphenyl-1-picryl hydrazyl (DPPH) radical scavenging activity and ferric-reducing power in the antioxidant assays. Total phenolic compounds, condensed tannins, gallotannins and flavonoids were quantitatively determined using spectrophotometric methods. Results At the screening assay concentration (0.25 μg/μl), 13 extracts showed good COX-1 inhibitory activity (>50%), while good activity was observed in 15 extracts against COX-2 enzyme. All the extracts of Crinum moorei (bulbs) showed good inhibition against both COX-1 and COX-2 enzymes. Though not significantly different ( P = 0.05), the highest COX-1 percentage inhibition (100%) was shown by Aloe ferox leaf PE and Colocasia antiquorum tuber DCM extracts, while Colocasia antiquorum tuber PE extract exhibited the highest (92.7%) percentage inhibition against COX-2. Crinum moorei bulb DCM extract showed the lowest EC 50 value (2.9 μg/ml) in the AChE assay. In addition, good to moderate bioactivities were observed in some extracts of Aloe ferox (leaves), Crinum moorei (bulbs) and Pycnostachys reticulata (leaves) in all the assays. The presence and/or amounts of phenolic compounds varied with plant species. Conclusion The results obtained in this study validate the use of the investigated medicinal plants in South African traditional medicine for pain-related ailments.