The Experts below are selected from a list of 1218 Experts worldwide ranked by ideXlab platform

Yan Wang - One of the best experts on this subject based on the ideXlab platform.

  • the use of masson s trichrome staining second harmonic imaging and two photon excited fluorescence of collagen in distinguishing intestinal tuberculosis from crohn s Disease
    Colorectal Disease, 2016
    Co-Authors: Pei-zhu Su, Liyun Huang, Haiting Yu, Yan Wang
    Abstract:

    Aim Differentiation between Crohns Disease (CD) and intestinal tuberculosis (ITB) continues to be difficult. The present study investigated the collagen fibre characteristics of CD and ITB using Masson's Trichrome staining and second harmonic generation imaging (SHG) and two-photon excited fluorescence (TPEF) imaging with the aim of distinguishing between them. Method The characteristics of collagen fibres in intestinal specimens from patients with CD, ITB and healthy controls were compared using Masson's Trichrome staining, SHI and TPEF imaging. Results Masson's Trichrome staining showed that the content of collagen fibre (540.92 [139.61-1681.93] vs 236.17 [72.94-1108.32], p<0.05) and fibre deposits (888.92 [315.89-3172.9] vs 498.98 [38.82-5802.31], p<0.05) were both higher in ITB than in CD. The content of collagen fibre (594.677 [139.61-1681.93] vs 107.425 [4.66-988.7], p<0.05) and fibre deposits (1118.4661 [315.89-5802.31] vs 340.575 [29.62-1188.87], p<0.05) were significantly higher in lesions with granuloma than those without. The SHG/TPEF images demonstrated that the percentage of fibrosis in ITB was also significantly higher than in CD (p<0.05), both in surgical (13.363±5.303% vs 8.322±5.078%, p=0.044)_and endoscopic specimens (mean rank 13.5 vs 7.5, p=0.023). The SHG/TPEF imaging described different distribution patterns of collagen between CD and ITB; in the former this was irregular in clumps while in ITB the collagen was arranged around caseating granulomata. Conclusion The evaluation of fibrosis in Crohns Disease and intestinal tuberculosis by Masson's Trichrome staining and SHG and TPEF imaging appear to distinguish between these two Diseases. This article is protected by copyright. All rights reserved.

  • the use of masson s trichrome staining second harmonic imaging and two photon excited fluorescence of collagen in distinguishing intestinal tuberculosis from crohn s Disease
    Colorectal Disease, 2016
    Co-Authors: Pei-zhu Su, Liyun Huang, Haiting Yu, Yan Wang
    Abstract:

    Aim Differentiation between Crohns Disease (CD) and intestinal tuberculosis (ITB) continues to be difficult. The present study investigated the collagen fibre characteristics of CD and ITB using Masson's Trichrome staining and second harmonic generation imaging (SHG) and two-photon excited fluorescence (TPEF) imaging with the aim of distinguishing between them. Method The characteristics of collagen fibres in intestinal specimens from patients with CD, ITB and healthy controls were compared using Masson's Trichrome staining, SHI and TPEF imaging. Results Masson's Trichrome staining showed that the content of collagen fibre (540.92 [139.61-1681.93] vs 236.17 [72.94-1108.32], p<0.05) and fibre deposits (888.92 [315.89-3172.9] vs 498.98 [38.82-5802.31], p<0.05) were both higher in ITB than in CD. The content of collagen fibre (594.677 [139.61-1681.93] vs 107.425 [4.66-988.7], p<0.05) and fibre deposits (1118.4661 [315.89-5802.31] vs 340.575 [29.62-1188.87], p<0.05) were significantly higher in lesions with granuloma than those without. The SHG/TPEF images demonstrated that the percentage of fibrosis in ITB was also significantly higher than in CD (p<0.05), both in surgical (13.363±5.303% vs 8.322±5.078%, p=0.044)_and endoscopic specimens (mean rank 13.5 vs 7.5, p=0.023). The SHG/TPEF imaging described different distribution patterns of collagen between CD and ITB; in the former this was irregular in clumps while in ITB the collagen was arranged around caseating granulomata. Conclusion The evaluation of fibrosis in Crohns Disease and intestinal tuberculosis by Masson's Trichrome staining and SHG and TPEF imaging appear to distinguish between these two Diseases. This article is protected by copyright. All rights reserved.

Pei-zhu Su - One of the best experts on this subject based on the ideXlab platform.

  • the use of masson s trichrome staining second harmonic imaging and two photon excited fluorescence of collagen in distinguishing intestinal tuberculosis from crohn s Disease
    Colorectal Disease, 2016
    Co-Authors: Pei-zhu Su, Liyun Huang, Haiting Yu, Yan Wang
    Abstract:

    Aim Differentiation between Crohns Disease (CD) and intestinal tuberculosis (ITB) continues to be difficult. The present study investigated the collagen fibre characteristics of CD and ITB using Masson's Trichrome staining and second harmonic generation imaging (SHG) and two-photon excited fluorescence (TPEF) imaging with the aim of distinguishing between them. Method The characteristics of collagen fibres in intestinal specimens from patients with CD, ITB and healthy controls were compared using Masson's Trichrome staining, SHI and TPEF imaging. Results Masson's Trichrome staining showed that the content of collagen fibre (540.92 [139.61-1681.93] vs 236.17 [72.94-1108.32], p<0.05) and fibre deposits (888.92 [315.89-3172.9] vs 498.98 [38.82-5802.31], p<0.05) were both higher in ITB than in CD. The content of collagen fibre (594.677 [139.61-1681.93] vs 107.425 [4.66-988.7], p<0.05) and fibre deposits (1118.4661 [315.89-5802.31] vs 340.575 [29.62-1188.87], p<0.05) were significantly higher in lesions with granuloma than those without. The SHG/TPEF images demonstrated that the percentage of fibrosis in ITB was also significantly higher than in CD (p<0.05), both in surgical (13.363±5.303% vs 8.322±5.078%, p=0.044)_and endoscopic specimens (mean rank 13.5 vs 7.5, p=0.023). The SHG/TPEF imaging described different distribution patterns of collagen between CD and ITB; in the former this was irregular in clumps while in ITB the collagen was arranged around caseating granulomata. Conclusion The evaluation of fibrosis in Crohns Disease and intestinal tuberculosis by Masson's Trichrome staining and SHG and TPEF imaging appear to distinguish between these two Diseases. This article is protected by copyright. All rights reserved.

  • the use of masson s trichrome staining second harmonic imaging and two photon excited fluorescence of collagen in distinguishing intestinal tuberculosis from crohn s Disease
    Colorectal Disease, 2016
    Co-Authors: Pei-zhu Su, Liyun Huang, Haiting Yu, Yan Wang
    Abstract:

    Aim Differentiation between Crohns Disease (CD) and intestinal tuberculosis (ITB) continues to be difficult. The present study investigated the collagen fibre characteristics of CD and ITB using Masson's Trichrome staining and second harmonic generation imaging (SHG) and two-photon excited fluorescence (TPEF) imaging with the aim of distinguishing between them. Method The characteristics of collagen fibres in intestinal specimens from patients with CD, ITB and healthy controls were compared using Masson's Trichrome staining, SHI and TPEF imaging. Results Masson's Trichrome staining showed that the content of collagen fibre (540.92 [139.61-1681.93] vs 236.17 [72.94-1108.32], p<0.05) and fibre deposits (888.92 [315.89-3172.9] vs 498.98 [38.82-5802.31], p<0.05) were both higher in ITB than in CD. The content of collagen fibre (594.677 [139.61-1681.93] vs 107.425 [4.66-988.7], p<0.05) and fibre deposits (1118.4661 [315.89-5802.31] vs 340.575 [29.62-1188.87], p<0.05) were significantly higher in lesions with granuloma than those without. The SHG/TPEF images demonstrated that the percentage of fibrosis in ITB was also significantly higher than in CD (p<0.05), both in surgical (13.363±5.303% vs 8.322±5.078%, p=0.044)_and endoscopic specimens (mean rank 13.5 vs 7.5, p=0.023). The SHG/TPEF imaging described different distribution patterns of collagen between CD and ITB; in the former this was irregular in clumps while in ITB the collagen was arranged around caseating granulomata. Conclusion The evaluation of fibrosis in Crohns Disease and intestinal tuberculosis by Masson's Trichrome staining and SHG and TPEF imaging appear to distinguish between these two Diseases. This article is protected by copyright. All rights reserved.

Haiting Yu - One of the best experts on this subject based on the ideXlab platform.

  • the use of masson s trichrome staining second harmonic imaging and two photon excited fluorescence of collagen in distinguishing intestinal tuberculosis from crohn s Disease
    Colorectal Disease, 2016
    Co-Authors: Pei-zhu Su, Liyun Huang, Haiting Yu, Yan Wang
    Abstract:

    Aim Differentiation between Crohns Disease (CD) and intestinal tuberculosis (ITB) continues to be difficult. The present study investigated the collagen fibre characteristics of CD and ITB using Masson's Trichrome staining and second harmonic generation imaging (SHG) and two-photon excited fluorescence (TPEF) imaging with the aim of distinguishing between them. Method The characteristics of collagen fibres in intestinal specimens from patients with CD, ITB and healthy controls were compared using Masson's Trichrome staining, SHI and TPEF imaging. Results Masson's Trichrome staining showed that the content of collagen fibre (540.92 [139.61-1681.93] vs 236.17 [72.94-1108.32], p<0.05) and fibre deposits (888.92 [315.89-3172.9] vs 498.98 [38.82-5802.31], p<0.05) were both higher in ITB than in CD. The content of collagen fibre (594.677 [139.61-1681.93] vs 107.425 [4.66-988.7], p<0.05) and fibre deposits (1118.4661 [315.89-5802.31] vs 340.575 [29.62-1188.87], p<0.05) were significantly higher in lesions with granuloma than those without. The SHG/TPEF images demonstrated that the percentage of fibrosis in ITB was also significantly higher than in CD (p<0.05), both in surgical (13.363±5.303% vs 8.322±5.078%, p=0.044)_and endoscopic specimens (mean rank 13.5 vs 7.5, p=0.023). The SHG/TPEF imaging described different distribution patterns of collagen between CD and ITB; in the former this was irregular in clumps while in ITB the collagen was arranged around caseating granulomata. Conclusion The evaluation of fibrosis in Crohns Disease and intestinal tuberculosis by Masson's Trichrome staining and SHG and TPEF imaging appear to distinguish between these two Diseases. This article is protected by copyright. All rights reserved.

  • the use of masson s trichrome staining second harmonic imaging and two photon excited fluorescence of collagen in distinguishing intestinal tuberculosis from crohn s Disease
    Colorectal Disease, 2016
    Co-Authors: Pei-zhu Su, Liyun Huang, Haiting Yu, Yan Wang
    Abstract:

    Aim Differentiation between Crohns Disease (CD) and intestinal tuberculosis (ITB) continues to be difficult. The present study investigated the collagen fibre characteristics of CD and ITB using Masson's Trichrome staining and second harmonic generation imaging (SHG) and two-photon excited fluorescence (TPEF) imaging with the aim of distinguishing between them. Method The characteristics of collagen fibres in intestinal specimens from patients with CD, ITB and healthy controls were compared using Masson's Trichrome staining, SHI and TPEF imaging. Results Masson's Trichrome staining showed that the content of collagen fibre (540.92 [139.61-1681.93] vs 236.17 [72.94-1108.32], p<0.05) and fibre deposits (888.92 [315.89-3172.9] vs 498.98 [38.82-5802.31], p<0.05) were both higher in ITB than in CD. The content of collagen fibre (594.677 [139.61-1681.93] vs 107.425 [4.66-988.7], p<0.05) and fibre deposits (1118.4661 [315.89-5802.31] vs 340.575 [29.62-1188.87], p<0.05) were significantly higher in lesions with granuloma than those without. The SHG/TPEF images demonstrated that the percentage of fibrosis in ITB was also significantly higher than in CD (p<0.05), both in surgical (13.363±5.303% vs 8.322±5.078%, p=0.044)_and endoscopic specimens (mean rank 13.5 vs 7.5, p=0.023). The SHG/TPEF imaging described different distribution patterns of collagen between CD and ITB; in the former this was irregular in clumps while in ITB the collagen was arranged around caseating granulomata. Conclusion The evaluation of fibrosis in Crohns Disease and intestinal tuberculosis by Masson's Trichrome staining and SHG and TPEF imaging appear to distinguish between these two Diseases. This article is protected by copyright. All rights reserved.

Liyun Huang - One of the best experts on this subject based on the ideXlab platform.

  • the use of masson s trichrome staining second harmonic imaging and two photon excited fluorescence of collagen in distinguishing intestinal tuberculosis from crohn s Disease
    Colorectal Disease, 2016
    Co-Authors: Pei-zhu Su, Liyun Huang, Haiting Yu, Yan Wang
    Abstract:

    Aim Differentiation between Crohns Disease (CD) and intestinal tuberculosis (ITB) continues to be difficult. The present study investigated the collagen fibre characteristics of CD and ITB using Masson's Trichrome staining and second harmonic generation imaging (SHG) and two-photon excited fluorescence (TPEF) imaging with the aim of distinguishing between them. Method The characteristics of collagen fibres in intestinal specimens from patients with CD, ITB and healthy controls were compared using Masson's Trichrome staining, SHI and TPEF imaging. Results Masson's Trichrome staining showed that the content of collagen fibre (540.92 [139.61-1681.93] vs 236.17 [72.94-1108.32], p<0.05) and fibre deposits (888.92 [315.89-3172.9] vs 498.98 [38.82-5802.31], p<0.05) were both higher in ITB than in CD. The content of collagen fibre (594.677 [139.61-1681.93] vs 107.425 [4.66-988.7], p<0.05) and fibre deposits (1118.4661 [315.89-5802.31] vs 340.575 [29.62-1188.87], p<0.05) were significantly higher in lesions with granuloma than those without. The SHG/TPEF images demonstrated that the percentage of fibrosis in ITB was also significantly higher than in CD (p<0.05), both in surgical (13.363±5.303% vs 8.322±5.078%, p=0.044)_and endoscopic specimens (mean rank 13.5 vs 7.5, p=0.023). The SHG/TPEF imaging described different distribution patterns of collagen between CD and ITB; in the former this was irregular in clumps while in ITB the collagen was arranged around caseating granulomata. Conclusion The evaluation of fibrosis in Crohns Disease and intestinal tuberculosis by Masson's Trichrome staining and SHG and TPEF imaging appear to distinguish between these two Diseases. This article is protected by copyright. All rights reserved.

  • the use of masson s trichrome staining second harmonic imaging and two photon excited fluorescence of collagen in distinguishing intestinal tuberculosis from crohn s Disease
    Colorectal Disease, 2016
    Co-Authors: Pei-zhu Su, Liyun Huang, Haiting Yu, Yan Wang
    Abstract:

    Aim Differentiation between Crohns Disease (CD) and intestinal tuberculosis (ITB) continues to be difficult. The present study investigated the collagen fibre characteristics of CD and ITB using Masson's Trichrome staining and second harmonic generation imaging (SHG) and two-photon excited fluorescence (TPEF) imaging with the aim of distinguishing between them. Method The characteristics of collagen fibres in intestinal specimens from patients with CD, ITB and healthy controls were compared using Masson's Trichrome staining, SHI and TPEF imaging. Results Masson's Trichrome staining showed that the content of collagen fibre (540.92 [139.61-1681.93] vs 236.17 [72.94-1108.32], p<0.05) and fibre deposits (888.92 [315.89-3172.9] vs 498.98 [38.82-5802.31], p<0.05) were both higher in ITB than in CD. The content of collagen fibre (594.677 [139.61-1681.93] vs 107.425 [4.66-988.7], p<0.05) and fibre deposits (1118.4661 [315.89-5802.31] vs 340.575 [29.62-1188.87], p<0.05) were significantly higher in lesions with granuloma than those without. The SHG/TPEF images demonstrated that the percentage of fibrosis in ITB was also significantly higher than in CD (p<0.05), both in surgical (13.363±5.303% vs 8.322±5.078%, p=0.044)_and endoscopic specimens (mean rank 13.5 vs 7.5, p=0.023). The SHG/TPEF imaging described different distribution patterns of collagen between CD and ITB; in the former this was irregular in clumps while in ITB the collagen was arranged around caseating granulomata. Conclusion The evaluation of fibrosis in Crohns Disease and intestinal tuberculosis by Masson's Trichrome staining and SHG and TPEF imaging appear to distinguish between these two Diseases. This article is protected by copyright. All rights reserved.

R M Beattie - One of the best experts on this subject based on the ideXlab platform.

  • g35 Crohns Disease initial treatment and outcomes at 12 months abstract g35 table 1
    Archives of Disease in Childhood, 2016
    Co-Authors: J Gavin, James J Ashton, N Heather, Luise V Marino, R M Beattie
    Abstract:

    Introduction Exclusive enteral nutrition (EEN) is the primary therapy for induction of remission and growth restoration in newly diagnosed children with Crohns Disease (CD). Patients who failed to go into clinical remission were offered steroids with partial enteral nutrition (PEN) alongside diet or steroids alone. The aim of this retrospective study was to investigate the effect of PEN/ steroids alone on growth outcome and relapse rate compared to EEN as induction therapy. Methods Basic clinical data including anthropometry, Paris classification, treatment type and outcome were collected at diagnosis, post induction therapy, at 3 months and at 1 year for 78 patients diagnosed from 2012–14. Clinical remission was determined using a physician global assessment and blood biochemistry. Relapse was defined as a change in medication due to worsening symptoms. Data are presented as median values. Results Age at diagnosis was 13 years (2–16 years), n = 62 (79%) were male. Patients received either EEN; n = 43;PEN; n = 16 or steroids alone; n = 19 as induction therapy. All groups had similar rates of clinical remission post induction therapy; 67% EEN, 75% PEN, 79% Steroids. There were no differences in relapse rates within 6 months; 30% EEN, 37% PEN, 47%. There was no difference between the mode of primary induction and Disease location or those who went on to require biological therapy (infliximab or adalimumab) EEN, 20%; PEN, 35.7% and Steroids 26.3%. There were no differences in height-for-age-z-scores (HAZ) at 1 year for any treatment group. Body mass index z-scores (BMIZ) had significantly improved in EEN and PEN groups at 1 year (EEN p Conclusion EEN and PEN primary induction treatments improved BMIZ. The EEN group displayed an improved HAZ at 1year. We do not demonstrate a superiority of any induction therapy on relapse rate.

  • g46 p maintenance enteral nutrition post induction therapy in paediatric Crohns Disease abstract g46 p table 1
    Archives of Disease in Childhood, 2016
    Co-Authors: J Gavin, James J Ashton, N Heather, Luise V Marino, R M Beattie
    Abstract:

    Introduction Several studies suggest that maintenance enteral nutrition (MEN) post induction in Crohns Disease (CD) may have a role in prolonging remission. This retrospective study compares growth outcomes and relapse rate in CD patients supplemented with MEN versus an unsupplemented group. Methods Data including anthropometry, treatment type and outcome were collected for 78 patients at diagnosis, start of MEN, at 3 months and at 1 year from 2012–14. Patients who received MEN (n = 42) post induction therapy were compared with those on normal diet (ND; n = 36). Clinical remission was determined using a physician global assessment and blood biochemistry. Relapse was defined as a change in medication due to symptoms. Data are presented as median values. Results Age at diagnosis was 13 years, 62/78 (79%) were male. All groups had similar rates of clinical remission post induction therapy. The length of MEN was 3 months. The energy content of MEN was 635kcals representing 33% of energy requirement. 32/42 (76%) MEN patients received EEN as induction therapy, 7/42 (17%) received PEN and 3/42 (7%) steroids. 24% of MEN group relapsed within 6 months of diagnosis versus 53% ND (p = 0.009). Body mass index z score (BMIZ) significantly improved in both groups (MEN: p Conclusion CD patients treated with MEN who received EEN or PEN as induction therapy were less likely to relapse within 6 months of diagnosis. BMIZ improved towards normal in both groups. Prolonged nutritional support from diagnosis may extend length of remission.