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Carlos Wilson Gomes Lopes - One of the best experts on this subject based on the ideXlab platform.

  • avaliacao das tecnicas de rotina no diagnostico de oocistos de Cryptosporidium baileyi em amostras de fezes de frangos de corte gallus gallus domesticus
    Revista Brasileira De Parasitologia Veterinaria, 2008
    Co-Authors: Sergian Vianna Cardozo, Walter Leira Teixeira Filho, Carlos Wilson Gomes Lopes
    Abstract:

    The objective of this work is to evaluate different diagnostic techniques for Cryptosporidium baileyi oocysts in broiler chickens fecal samples infected na...

  • experimental transmission of Cryptosporidium baileyi apicomplexa cryptosporidiidae isolated of broiler chicken to japanese quail coturnix japonica
    Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia , 2005
    Co-Authors: Sergian V Cardozo, Carlos Wilson Gomes Lopes
    Abstract:

    In this work, oocysts of Cryptosporidium baileyi were isolated and identified in broiler chickens from three different Municipalities of the State of Rio de Janeiro, where they were isolated and identified by using the centrifuge- flotation technique associated to bright-field. Staining techniques, such as: modified Ziehl-Neelsen and safranin-methylene blue, were carried out to confirm natural infection. Oocysts of C. baileyi from broiler chickens were able to infect Coccidia-free Japanese quails, by observation of endogenous stages at histological sections, and the elimination of oocysts in the feces with prepatent period of seven days and patent period of 21 days after infection. Oocysts of C. baileyi from broiler chickens and Japanese quails were similar on bright-field microscopy. With respect to the staining techniques used in this research, all of them left to significant changes in length and width of oocysts, but shape indexes were maintained. Bright-field microscopy was the best technique for oocysts comparison shed by broiler chickens and Japanese quail because of no different among oocysts were observed.

  • transmissao experimental de Cryptosporidium baileyi apicomplexa cryptosporidiidae isolado de frango de corte a codorna japonesa coturnix japonica
    Revista Brasileira De Parasitologia Veterinaria, 2005
    Co-Authors: Sergian V Cardozo, Walter Leira Teixeira Filho, Carlos Wilson Gomes Lopes
    Abstract:

    2 ABSTRACT: - CARDOZO, S.V.; TEIXEIRA FILHO, W.L.; LOPES, C.W.G. (Experimental transmission of Cryptosporidum baileyi (Apicomplexa: Cryptosporidiidae) isolated of broiler chicken to Japanese quail (Coturnix japonica)). Transmissao experimental de Cryptosporidium baileyi (Apicomplexa: Cryptoporidiidae) isolado de frango de corte a codorna japonesa. Revista Brasileira de Parasitologia Veterinaria, v. 14, n. 3, p. 119-124, 2005. Universidade Federal Rural do Rio de Janeiro, Departamento de Parasitologia Animal, Km 7 da BR 465, Seropedica, RJ, Brazil, 23890-000. E-mail: lopescwg@ufrrj.br In this work, oocysts of Cryptosporidium baileyi were isolated and identified in broiler chickens from three different Municipalities of the State of Rio de Janeiro, where they were isolated and identified by using the centrifuge- flotation technique associated to bright-field. Staining techniques, such as: modified Ziehl-Neelsen and safranin- methylene blue, were carried out to confirm natural infection. Oocysts of C. baileyi from broiler chickens were able to infect Coccidia-free Japanese quails, by observation of endogenous stages at histological sections, and the elimination of oocysts in the feces with prepatent period of seven days and patent period of 21 days after infection. Oocysts of C. baileyi from broiler chickens and Japanese quails were similar on bright-field microscopy. With respect to the staining techniques used in this research, all of them left to significant changes in length and width of oocysts, but shape indexes were maintained. Bright-field microscopy was the best techinique for oocysts comparison shed by broiler chickens and Japanese quail because of no different among oocysts were observed.

Ivan Pavlasek - One of the best experts on this subject based on the ideXlab platform.

  • protozoan and helminth infections of aviary reared alectoris rufa galliformes phasianidae before releasing for hunting in the czech republic infection dynamics and potential risks
    Journal of Parasitology, 2020
    Co-Authors: Ondřej Maca, Ivan Pavlasek
    Abstract:

    Age-related dynamics of protozoan and helminth infections in the red-legged partridge, Alectoris rufa, were studied in an intensive breeding aviary in the Czech Republic before releasing birds for hunting purposes. Pooled fecal samples (n = 900) were examined over 3 rearing seasons (2012-2014). A total of 4 protozoan species, Cryptosporidium baileyi, Cryptosporidium meleagridis, Eimeria kofoidi, and Eimeria legionensis, and 4 helminths, Capillaria phasianina, Eucoleus perforans, Heterakis gallinarum, and Syngamus trachea, was found. The most common parasite was S. trachea (prevalence 20-26%) in dead birds (n = 99), which represents a high risk for breeders. Co-occurrence of protozoans and helminths indicated similarities in infection dynamics throughout the 3 breeding seasons. Mixed infections of Cryptosporidium baileyi and C. meleagridis with other parasitic species are reported for the first time. Our findings provide new insights into breeding of A. rufa and may help to improve the efficacy of disease control strategies and prevention, especially with the potential for spreading of parasitic infections to wildlife through released birds into open areas.

  • first finding of spontaneous infections with Cryptosporidium baileyi and c meleagridis in the red legged partridge alectoris rufa from an aviary in the czech republic
    Veterinary Parasitology, 2015
    Co-Authors: Ondřej Maca, Ivan Pavlasek
    Abstract:

    Abstract This paper represents the first report of spontaneous infection with Cryptosporidium baileyi and Cryptosporidium meleagridis in the red-legged partridge (Alectoris rufa), as well as the percentage of positive samples and age-associated dynamics of cryptosporidial infections in an aviary in the Czech Republic. The entire infection process was monitored over two semesters (July–December 2012 and 2013) until release of birds for hunting purposes. Coprological examination of 663 pooled fecal samples and 89 post-mortem examinations of red-legged partridges were carried out. Our results indicated that infections with C. baileyi only occurred in 5–7 week-old birds during 2013 (percentage of positivity, 1%) and those with C. meleagridis in 18–22 week (17%) and 17–21 week-old birds (24%) during 2012 and 2013, respectively. Molecular characterization of isolates of C. baileyi and C. meleagridis heat shock protein 70 and actin genes were analyzed in order to support our coprological results. DNA sequence analysis of the 60 kDa glycoprotein gene was used to subtype C. meleagridis. Our findings extend the host range for C. baileyi.

Ondřej Maca - One of the best experts on this subject based on the ideXlab platform.

  • protozoan and helminth infections of aviary reared alectoris rufa galliformes phasianidae before releasing for hunting in the czech republic infection dynamics and potential risks
    Journal of Parasitology, 2020
    Co-Authors: Ondřej Maca, Ivan Pavlasek
    Abstract:

    Age-related dynamics of protozoan and helminth infections in the red-legged partridge, Alectoris rufa, were studied in an intensive breeding aviary in the Czech Republic before releasing birds for hunting purposes. Pooled fecal samples (n = 900) were examined over 3 rearing seasons (2012-2014). A total of 4 protozoan species, Cryptosporidium baileyi, Cryptosporidium meleagridis, Eimeria kofoidi, and Eimeria legionensis, and 4 helminths, Capillaria phasianina, Eucoleus perforans, Heterakis gallinarum, and Syngamus trachea, was found. The most common parasite was S. trachea (prevalence 20-26%) in dead birds (n = 99), which represents a high risk for breeders. Co-occurrence of protozoans and helminths indicated similarities in infection dynamics throughout the 3 breeding seasons. Mixed infections of Cryptosporidium baileyi and C. meleagridis with other parasitic species are reported for the first time. Our findings provide new insights into breeding of A. rufa and may help to improve the efficacy of disease control strategies and prevention, especially with the potential for spreading of parasitic infections to wildlife through released birds into open areas.

  • first finding of spontaneous infections with Cryptosporidium baileyi and c meleagridis in the red legged partridge alectoris rufa from an aviary in the czech republic
    Veterinary Parasitology, 2015
    Co-Authors: Ondřej Maca, Ivan Pavlasek
    Abstract:

    Abstract This paper represents the first report of spontaneous infection with Cryptosporidium baileyi and Cryptosporidium meleagridis in the red-legged partridge (Alectoris rufa), as well as the percentage of positive samples and age-associated dynamics of cryptosporidial infections in an aviary in the Czech Republic. The entire infection process was monitored over two semesters (July–December 2012 and 2013) until release of birds for hunting purposes. Coprological examination of 663 pooled fecal samples and 89 post-mortem examinations of red-legged partridges were carried out. Our results indicated that infections with C. baileyi only occurred in 5–7 week-old birds during 2013 (percentage of positivity, 1%) and those with C. meleagridis in 18–22 week (17%) and 17–21 week-old birds (24%) during 2012 and 2013, respectively. Molecular characterization of isolates of C. baileyi and C. meleagridis heat shock protein 70 and actin genes were analyzed in order to support our coprological results. DNA sequence analysis of the 60 kDa glycoprotein gene was used to subtype C. meleagridis. Our findings extend the host range for C. baileyi.

Sergian V Cardozo - One of the best experts on this subject based on the ideXlab platform.

  • experimental transmission of Cryptosporidium baileyi apicomplexa cryptosporidiidae isolated of broiler chicken to japanese quail coturnix japonica
    Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia , 2005
    Co-Authors: Sergian V Cardozo, Carlos Wilson Gomes Lopes
    Abstract:

    In this work, oocysts of Cryptosporidium baileyi were isolated and identified in broiler chickens from three different Municipalities of the State of Rio de Janeiro, where they were isolated and identified by using the centrifuge- flotation technique associated to bright-field. Staining techniques, such as: modified Ziehl-Neelsen and safranin-methylene blue, were carried out to confirm natural infection. Oocysts of C. baileyi from broiler chickens were able to infect Coccidia-free Japanese quails, by observation of endogenous stages at histological sections, and the elimination of oocysts in the feces with prepatent period of seven days and patent period of 21 days after infection. Oocysts of C. baileyi from broiler chickens and Japanese quails were similar on bright-field microscopy. With respect to the staining techniques used in this research, all of them left to significant changes in length and width of oocysts, but shape indexes were maintained. Bright-field microscopy was the best technique for oocysts comparison shed by broiler chickens and Japanese quail because of no different among oocysts were observed.

  • transmissao experimental de Cryptosporidium baileyi apicomplexa cryptosporidiidae isolado de frango de corte a codorna japonesa coturnix japonica
    Revista Brasileira De Parasitologia Veterinaria, 2005
    Co-Authors: Sergian V Cardozo, Walter Leira Teixeira Filho, Carlos Wilson Gomes Lopes
    Abstract:

    2 ABSTRACT: - CARDOZO, S.V.; TEIXEIRA FILHO, W.L.; LOPES, C.W.G. (Experimental transmission of Cryptosporidum baileyi (Apicomplexa: Cryptosporidiidae) isolated of broiler chicken to Japanese quail (Coturnix japonica)). Transmissao experimental de Cryptosporidium baileyi (Apicomplexa: Cryptoporidiidae) isolado de frango de corte a codorna japonesa. Revista Brasileira de Parasitologia Veterinaria, v. 14, n. 3, p. 119-124, 2005. Universidade Federal Rural do Rio de Janeiro, Departamento de Parasitologia Animal, Km 7 da BR 465, Seropedica, RJ, Brazil, 23890-000. E-mail: lopescwg@ufrrj.br In this work, oocysts of Cryptosporidium baileyi were isolated and identified in broiler chickens from three different Municipalities of the State of Rio de Janeiro, where they were isolated and identified by using the centrifuge- flotation technique associated to bright-field. Staining techniques, such as: modified Ziehl-Neelsen and safranin- methylene blue, were carried out to confirm natural infection. Oocysts of C. baileyi from broiler chickens were able to infect Coccidia-free Japanese quails, by observation of endogenous stages at histological sections, and the elimination of oocysts in the feces with prepatent period of seven days and patent period of 21 days after infection. Oocysts of C. baileyi from broiler chickens and Japanese quails were similar on bright-field microscopy. With respect to the staining techniques used in this research, all of them left to significant changes in length and width of oocysts, but shape indexes were maintained. Bright-field microscopy was the best techinique for oocysts comparison shed by broiler chickens and Japanese quail because of no different among oocysts were observed.

Guanghui Zhao - One of the best experts on this subject based on the ideXlab platform.

  • serum metabolomics in chickens infected with Cryptosporidium baileyi
    Parasites & Vectors, 2021
    Co-Authors: Xin Yang, Longxian Zhang, Xiancheng Fan, Xi Chen, Yuxin Wang, Junke Song, Guanghui Zhao
    Abstract:

    Cryptosporidium baileyi is an economically important zoonotic pathogen that causes serious respiratory symptoms in chickens for which no effective control measures are currently available. An accumulating body of evidence indicates the potential and usefulness of metabolomics to further our understanding of the interaction between pathogens and hosts, and to search for new diagnostic or pharmacological biomarkers of complex microorganisms. The aim of this study was to identify the impact of C. baileyi infection on the serum metabolism of chickens and to assess several metabolites as potential diagnostic biomarkers for C. baileyi infection. Ultraperformance liquid chromatography-mass spectrometry (UPLC-MS) and subsequent multivariate statistical analysis were applied to investigate metabolomics profiles in the serum samples of chickens infected with C. baileyi, and to identify potential metabolites that can be used to distinguish chickens infected with C. baileyi from non-infected birds. Multivariate statistical analysis identified 138 differential serum metabolites between mock- and C. baileyi-infected chickens at 5 days post-infection (dpi), including 115 upregulated and 23 downregulated compounds. These metabolites were significantly enriched into six pathways, of which two pathways associated with energy and lipid metabolism, namely glycerophospholipid metabolism and sphingolipid metabolism, respectively, were the most enriched. Interestingly, some important immune-related pathways were also significantly enriched, including the intestinal immune network for IgA production, autophagy and cellular senescence. Nine potential C. baileyi-responsive metabolites were identified, including choline, sirolimus, all-trans retinoic acid, PC(14:0/22:1(13Z)), PC(15:0/22:6(4Z,7Z,10Z,13Z,16Z,19Z)), PE(16:1(9Z)/24:1(15Z)), phosphocholine, SM(d18:0/16:1(9Z)(OH)) and sphinganine. This is the first report on serum metabolic profiling of chickens with early-stage C. baileyi infection. The results provide novel insights into the pathophysiological mechanisms of C. baileyi in chickens.

  • Serum metabolomics in chickens infected with Cryptosporidium baileyi
    'Springer Science and Business Media LLC', 2021
    Co-Authors: Xin Yang, Longxian Zhang, Xiancheng Fan, Xi Chen, Yuxin Wang, Junke Song, Guanghui Zhao
    Abstract:

    Abstract Background Cryptosporidium baileyi is an economically important zoonotic pathogen that causes serious respiratory symptoms in chickens for which no effective control measures are currently available. An accumulating body of evidence indicates the potential and usefulness of metabolomics to further our understanding of the interaction between pathogens and hosts, and to search for new diagnostic or pharmacological biomarkers of complex microorganisms. The aim of this study was to identify the impact of C. baileyi infection on the serum metabolism of chickens and to assess several metabolites as potential diagnostic biomarkers for C. baileyi infection. Methods Ultraperformance liquid chromatography-mass spectrometry (UPLC-MS) and subsequent multivariate statistical analysis were applied to investigate metabolomics profiles in the serum samples of chickens infected with C. baileyi, and to identify potential metabolites that can be used to distinguish chickens infected with C. baileyi from non-infected birds. Results Multivariate statistical analysis identified 138 differential serum metabolites between mock- and C. baileyi-infected chickens at 5 days post-infection (dpi), including 115 upregulated and 23 downregulated compounds. These metabolites were significantly enriched into six pathways, of which two pathways associated with energy and lipid metabolism, namely glycerophospholipid metabolism and sphingolipid metabolism, respectively, were the most enriched. Interestingly, some important immune-related pathways were also significantly enriched, including the intestinal immune network for IgA production, autophagy and cellular senescence. Nine potential C. baileyi-responsive metabolites were identified, including choline, sirolimus, all-trans retinoic acid, PC(14:0/22:1(13Z)), PC(15:0/22:6(4Z,7Z,10Z,13Z,16Z,19Z)), PE(16:1(9Z)/24:1(15Z)), phosphocholine, SM(d18:0/16:1(9Z)(OH)) and sphinganine. Conclusions This is the first report on serum metabolic profiling of chickens with early-stage C. baileyi infection. The results provide novel insights into the pathophysiological mechanisms of C. baileyi in chickens. Graphic abstrac

  • genome wide analysis of differentially expressed profiles of mrnas lncrnas and circrnas during Cryptosporidium baileyi infection
    BMC Genomics, 2018
    Co-Authors: Guanjing Ren, Xiancheng Fan, Tingli Liu, Shasha Wang, Guanghui Zhao
    Abstract:

    Cryptosporidium baileyi is the most common Cryptosporidium species in birds. However, effective prevention measures and treatment for C. baileyi infection were still not available. Long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) play important roles in regulating occurrence and progression of many diseases and are identified as effective biomarkers for diagnosis and prognosis of several diseases. In the present study, the expression profiles of host mRNAs, lncRNAs and circRNAs associated with C. baileyi infection were investigated for the first time. The tracheal tissues of experimental (C. baileyi infection) and control chickens were collected for deep RNA sequencing, and 545,479,934 clean reads were obtained. Of them, 1376 novel lncRNAs were identified, including 1161 long intergenic non-coding RNAs (lincRNAs) and 215 anti-sense lncRNAs. A total of 124 lncRNAs were found to be significantly differentially expressed between the experimental and control groups. Additionally, 14,698 mRNAs and 9085 circRNAs were identified, and significantly different expressions were observed for 1317 mRNAs and 104 circRNAs between two groups. Bioinformatic analyses of gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway for their targets and source genes suggested that these dysregulated genes may be involved in the interaction between the host and C. baileyi. The present study revealed the expression profiles of mRNAs, lncRNAs and circRNAs during C. baileyi infection for the first time, and sheds lights on the roles of lncRNAs and circRNAs underlying the pathogenesis of Cryptosporidium infection.

  • Genome-wide analysis of differentially expressed profiles of mRNAs, lncRNAs and circRNAs during Cryptosporidium baileyi infection
    'Springer Science and Business Media LLC', 2018
    Co-Authors: Guanjing Ren, Xiancheng Fan, Tingli Liu, Shasha Wang, Guanghui Zhao
    Abstract:

    Abstract Background Cryptosporidium baileyi is the most common Cryptosporidium species in birds. However, effective prevention measures and treatment for C. baileyi infection were still not available. Long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) play important roles in regulating occurrence and progression of many diseases and are identified as effective biomarkers for diagnosis and prognosis of several diseases. In the present study, the expression profiles of host mRNAs, lncRNAs and circRNAs associated with C. baileyi infection were investigated for the first time. Results The tracheal tissues of experimental (C. baileyi infection) and control chickens were collected for deep RNA sequencing, and 545,479,934 clean reads were obtained. Of them, 1376 novel lncRNAs were identified, including 1161 long intergenic non-coding RNAs (lincRNAs) and 215 anti-sense lncRNAs. A total of 124 lncRNAs were found to be significantly differentially expressed between the experimental and control groups. Additionally, 14,698 mRNAs and 9085 circRNAs were identified, and significantly different expressions were observed for 1317 mRNAs and 104 circRNAs between two groups. Bioinformatic analyses of gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway for their targets and source genes suggested that these dysregulated genes may be involved in the interaction between the host and C. baileyi. Conclusions The present study revealed the expression profiles of mRNAs, lncRNAs and circRNAs during C. baileyi infection for the first time, and sheds lights on the roles of lncRNAs and circRNAs underlying the pathogenesis of Cryptosporidium infection

  • the expression dynamics of il 17 and th17 response relative cytokines in the trachea and spleen of chickens after infection with Cryptosporidium baileyi
    Parasites & Vectors, 2014
    Co-Authors: Guanghui Zhao, Wenyu Cheng, Wan Wang, Yanqing Jia, Yanqin Fang
    Abstract:

    Cryptosporidium baileyi is the dominant Cryptosporidium species in birds causing emerging health problems in the poultry industry, and is also a model to study the biology of Cryptosporidium spp.. IL-17 (also called IL-17A) is a hallmark pro-inflammatory cytokine of Th17 cells that plays an important role in several human autoimmune diseases and microbial infection disease of many animals, and it may play a role in Cryptosporidium infection. The present study examined the mRNA level of IL-17 and Th17 response relative cytokines in the trachea and spleen of C. baileyi-infected chickens at different time points using real-time quantitative PCR (qPCR). All examined cytokines in the trachea were up-regulated in the infected chickens compared with the uninfected control during C. baileyi infection. Significant increased IL-17 mRNA level in the trachea was observed as early as 12 h post infection (pi), peaking at 24 h pi and 10 d pi, and declining thereafter. The transcription levels of IL-17 and Th17 response relative cytokines in spleen were also significantly increased at different time points during the infection. IL-17 was indicated to participate in the induction of inflammation during infection of some intracellular protozoan parasites. The results in the present study suggest that IL-17 may play a role in immunity against Cryptosporidium infection, and provide basic information for determining the role of Th17 cell in Cryptosporidium infection.