The Experts below are selected from a list of 1146 Experts worldwide ranked by ideXlab platform
A Tsonispanagiotis - One of the best experts on this subject based on the ideXlab platform.
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Intrinsic lens forming potential of mouse lens Epithelial versus newt iris pigment Epithelial Cells in three-dimensional culture.
Tissue engineering. Part C Methods, 2013Co-Authors: Hoffmannandrea, Nakamurakenta, A TsonispanagiotisAbstract:Adult newts (Notophthalmus viridescens) are capable of complete lens regeneration that is mediated through dorsal iris pigment Epithelial (IPE) Cells transdifferentiation. In contrast, higher vertebrates such as mice demonstrate only limited lens regeneration in the presence of an intact lens capsule with remaining lens Epithelial Cells. To compare the intrinsic lens regeneration potential of newt IPE versus mouse lens Epithelial Cells (MLE), we have established a novel culture method that uses Cell aggregation before culture in growth factor-reduced Matrigel™. Dorsal newt IPE aggregates demonstrated complete lens formation within 1 to 2 weeks of Matrigel culture without basic fibroblast growth factor (bFGF) supplementation, including the establishment of a peripheral Cuboidal Epithelial Cell layer, and the appearance of central lens fibers that were positive for αA-crystallin. In contrast, the lens-forming potential of MLE Cell aggregates cultured in Matrigel was incomplete and resulted in the formation ...
Hoffmannandrea - One of the best experts on this subject based on the ideXlab platform.
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Intrinsic lens forming potential of mouse lens Epithelial versus newt iris pigment Epithelial Cells in three-dimensional culture.
Tissue engineering. Part C Methods, 2013Co-Authors: Hoffmannandrea, Nakamurakenta, A TsonispanagiotisAbstract:Adult newts (Notophthalmus viridescens) are capable of complete lens regeneration that is mediated through dorsal iris pigment Epithelial (IPE) Cells transdifferentiation. In contrast, higher vertebrates such as mice demonstrate only limited lens regeneration in the presence of an intact lens capsule with remaining lens Epithelial Cells. To compare the intrinsic lens regeneration potential of newt IPE versus mouse lens Epithelial Cells (MLE), we have established a novel culture method that uses Cell aggregation before culture in growth factor-reduced Matrigel™. Dorsal newt IPE aggregates demonstrated complete lens formation within 1 to 2 weeks of Matrigel culture without basic fibroblast growth factor (bFGF) supplementation, including the establishment of a peripheral Cuboidal Epithelial Cell layer, and the appearance of central lens fibers that were positive for αA-crystallin. In contrast, the lens-forming potential of MLE Cell aggregates cultured in Matrigel was incomplete and resulted in the formation ...
Nakamurakenta - One of the best experts on this subject based on the ideXlab platform.
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Intrinsic lens forming potential of mouse lens Epithelial versus newt iris pigment Epithelial Cells in three-dimensional culture.
Tissue engineering. Part C Methods, 2013Co-Authors: Hoffmannandrea, Nakamurakenta, A TsonispanagiotisAbstract:Adult newts (Notophthalmus viridescens) are capable of complete lens regeneration that is mediated through dorsal iris pigment Epithelial (IPE) Cells transdifferentiation. In contrast, higher vertebrates such as mice demonstrate only limited lens regeneration in the presence of an intact lens capsule with remaining lens Epithelial Cells. To compare the intrinsic lens regeneration potential of newt IPE versus mouse lens Epithelial Cells (MLE), we have established a novel culture method that uses Cell aggregation before culture in growth factor-reduced Matrigel™. Dorsal newt IPE aggregates demonstrated complete lens formation within 1 to 2 weeks of Matrigel culture without basic fibroblast growth factor (bFGF) supplementation, including the establishment of a peripheral Cuboidal Epithelial Cell layer, and the appearance of central lens fibers that were positive for αA-crystallin. In contrast, the lens-forming potential of MLE Cell aggregates cultured in Matrigel was incomplete and resulted in the formation ...
Chikako Itano - One of the best experts on this subject based on the ideXlab platform.
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Cell differentiation of alveolar epithelium in the developing rat lung: ultrahistochemical studies of glycoconjugates on the Epithelial Cell surface
Histochemistry, 1993Co-Authors: Hirohiko Iwatsuki, Kazunobu Sasaki, Masumi Suda, Chikako ItanoAbstract:Glycoconjugates on the surface of pulmonary Epithelial Cells were ultrahistochemically examined in the fetal, neonatal and adult rat lung. Lectin and colloidal iron staining procedures were performed in combination with digestion using carbohydrate-degrading enzymes or methylation. The glycoconjugate composition of columnar Cells at 16 days gestation was similar to that of Cuboidal Cells at 19 days gestation. Glycoconjugate differentiation on the Cell surface occurred at 20 days gestation, and especially the loss of soybean agglutinin (SBA) binding sites could be detected on type II Cells. The contents of Ricinus communis agglutinin-I (RCA-I) and Concanavalin A (Con A) binding sites on type II Cells also began to decrease. On the contrary, the content of sulfated saccharides decreased on the surface of type I Cells during development. Glycoconjugate differentiation on both type I and II Cells was completed with the disappearance of hyaluronic acid and peanut agglutinin (PNA) binding sites; type I and II Cells acquired a similar histochemical composition to that on adult type I and II Cells at 5 days after birth. Both type I and II Cells share a common early precursor Cell, that is, the Cuboidal Epithelial Cell at the canalicular stage.
Philip S. Rudland - One of the best experts on this subject based on the ideXlab platform.
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Relationship of growth factors and differentiation in normal and neoplastic development of the mammary gland.
Cancer treatment and research, 1991Co-Authors: David G. Fernig, John A. Smith, Philip S. RudlandAbstract:The mammary gland of nonpregnant mammals is composed of an epithelium embedded in a fatty stroma. The epithelium consists of a branching ductal tree terminating in alveolar buds (ABs) in rats or in terminal ductal- lobuloalveolar units (TDLUs) in humans [1, 2]. The boundary of the epithelium is formed by a basement membrane, on the inner surface of which is a more or less continuous layer of elongated myoEpithelial Cells possessing smooth muscle-like myofilaments and pinocytotic vesicles [3–6]. One or more layers of Cuboidal Epithelial Cells constitute the core of the ducts, with the inner layer bordering a lumen that is continuous throughout the ductal tree [7]. The luminal, Cuboidal Epithelial Cells have apical microvilli and specialized junctional complexes with associated desmosomes. In the terminal ABs and TDLUs that form distended lobules, the luminal layer is composed of secretory or alveolar Cells that synthesize and secrete milk products during lactation [2, 8]. More recently a battery of immunocytochemical probes has been used to define, on a more molecular basis, the Cuboidal Epithelial Cell of the ducts, the Epithelial Cells of the ABs/TDLUs, the myoEpithelial Cells, and potential transitional Cells [9–11]. These probes have been important in understanding the developmental relationship between the different Cell types found in the mature mammary gland.