The Experts below are selected from a list of 321087 Experts worldwide ranked by ideXlab platform
Sebastiaan Mastenbroek - One of the best experts on this subject based on the ideXlab platform.
-
Culture Media for human pre implantation embryos in assisted reproductive technology cycles
Cochrane Database of Systematic Reviews, 2015Co-Authors: Mohamed M Youssef, Eleni Mantikou, Hesham Alinany, Sjoerd Repping, Madelon Van Wely, Fulco Van Der Veen, Sebastiaan MastenbroekAbstract:Background Many Media are commercially available for culturing pre-implantation human embryos in assisted reproductive technology (ART) cycles. It is unknown which Culture medium leads to the best success rates after ART. Objectives To evaluate the safety and effectiveness of different human pre-implantation embryo Culture Media in used for in vitro fertilisation (IVF) and intracytoplasmic sperm injection (ICSI) cycles. Search methods We searched the Cochrane Menstrual Disorders and Subfertility Group's Trials Register, Cochrane Central Register of Controlled Trials (CENTRAL), MEDLINE, EMBASE, the National Research Register, the Medical Research Council's Clinical Trials Register and the NHS Center for Reviews and Dissemination databases from January 1985 to March 2015. We also examined the reference lists of all known primary studies, review articles, citation lists of relevant publications and abstracts of major scientific meetings. Selection criteria We included all randomised controlled trials which randomised women, oocytes or embryos and compared any two commercially available Culture Media for human pre-implantation embryos in an IVF or ICSI programme. Data collection and analysis Two review authors independently selected the studies, assessed their risk of bias and extracted data. We sought additional information from the authors if necessary. We assessed the quality of the evidence using Grades of Recommendation, Assessment, Development and Evaluation (GRADE) methods. The primary review outcome was live birth or ongoing pregnancy. Main results We included 32 studies in this review. Seventeen studies randomised women (total 3666), three randomised cycles (total 1018) and twelve randomised oocytes (over 15,230). It was not possible to pool any of the data because each study compared different Culture Media. Only seven studies reported live birth or ongoing pregnancy. Four of these studies found no evidence of a difference between the Media compared, for either day three or day five embryo transfer. The data from the fifth study did not appear reliable. Six studies reported clinical pregnancy rate. One of these found a difference between the Media compared, suggesting that for cleavage-stage embryo transfer, Quinn's Advantage was associated with higher clinical pregnancy rates than G5 (odds ratio (OR) 1.56; 95% confidence interval (CI) 1.12 to 2.16; 692 women). This study was available only as an abstract and the quality of the evidence was low. With regards to adverse effects, three studies reported multiple pregnancies and six studies reported miscarriage. None of them found any evidence of a difference between the Culture Media used. None of the studies reported on the health of offspring. Most studies (22/32) failed to report their source of funding and none described their methodology in adequate detail. The overall quality of the evidence was rated as very low for nearly all comparisons, the main limitations being imprecision and poor reporting of study methods. Authors' conclusions An optimal embryo Culture medium is important for embryonic development and subsequently the success of IVF or ICSI treatment. There has been much controversy about the most appropriate embryo Culture medium. Numerous studies have been performed, but no two studies compared the same Culture Media and none of them found any evidence of a difference between the Culture Media used. We conclude that there is insufficient evidence to support or refute the use of any specific Culture medium. Properly designed and executed randomised trials are necessary.
-
embryo Culture Media and ivf icsi success rates a systematic review
Human Reproduction Update, 2013Co-Authors: Eleni Mantikou, Mohamed Youssef, M Van Wely, F Van Der Veen, Hesham Alinany, Sjoerd Repping, Sebastiaan MastenbroekAbstract:BACKGROUND The Media that are used to Culture human preimplantation embryos are considered to be an important factor for the success rates of IVF/ICSI. Here, we present a systematic review of randomized controlled trials (RCTs) on the effect of Culture Media on IVF/ICSI success rates. METHODS RCTs published between January 1985 and July 2012 were eligible for inclusion. The primary outcome was live birth. Secondary outcomes were health of babies born, ongoing pregnancies, clinical pregnancies, miscarriages, multiple pregnancies, implantation rate, cryopreservation rate, embryo quality and fertilization rate. For those Media that were evaluated in more than one comparison, an unconventional meta-analysis was performed by pooling the data of the Media they were compared to. RESULTS Twenty-two RCTs were included that evaluated 31 different comparisons. Conventional meta-analysis was not possible for any of the outcomes as nearly all trials compared different Culture Media. Only four trials reported on live birth, and one of them reported a significant difference. Nine trials reported on ongoing and/or clinical pregnancy rates, of which four showed a significant difference. Pooling the data did not reveal a superior Culture medium. CONCLUSIONS It is yet unknown what Culture medium leads to the best success rates in IVF/ICSI. Given the potential importance of Culture Media for treatment outcome, rigorously designed RCTs are needed for currently available, as well as newly introduced Culture Media.
En Yang - One of the best experts on this subject based on the ideXlab platform.
-
influence of Culture Media ph and temperature on growth and bacteriocin production of bacteriocinogenic lactic acid bacteria
AMB Express, 2018Co-Authors: En Yang, Yueming Jiang, Craig Doucette, Sherry Fillmore, Bradley WalkerAbstract:There has been continued interest in bacteriocins research from an applied perspective as bacteriocins have potential to be used as natural preservative. Four bacteriocinogenic lactic acid bacteria (LAB) strains of Lactobacillus curvatus (Arla-10), Enterococcus faecium (JFR-1), Lactobacillus paracasei subsp. paracasei (JFR-5) and Streptococcus thermophilus (TSB-8) were previously isolated and identified in our lab. The objective of this study was to determine the optimal growth conditions for both LAB growth and bacteriocins production. In this study, various growth conditions including Culture Media (MRS and BHI), initial pH of Culture Media (4.5, 5.5, 6.2, 7.4 and 8.5), and incubation temperatures (20, 37 and 44 °C) were investigated for LAB growth measured as optical density (OD), bacteriocin activity determined as arbitrary unit and viability of LAB expressed as log CFU ml−1. Growth curves of the bacteriocinogenic LAB were generated using a Bioscreen C. Our results indicated that Arla-10, JFR-1, and JFR-5 strains grew well on both MRS and BHI Media at growth temperature tested whereas TSB-8 strain, unable to grow at 20 °C. LAB growth was significantly affected by the initial pH of Culture Media (p < 0.001) and the optimal pH was found ranging from 6.2 to 8.5. Bacteriocin activity was significantly different in MRS versus BHI (p < 0.001), and the optimal condition for LAB to produce bacteriocins was determined in MRS broth, pH 6.2 at 37 °C. This study provides useful information on potential application of bacteriocinogenic LAB in food fermentation processes.
-
Influence of Culture Media, pH and temperature on growth and bacteriocin production of bacteriocinogenic lactic acid bacteria
SpringerOpen, 2018Co-Authors: En Yang, Lihua Fan, Jinping Yan, Yueming Jiang, Craig Doucette, Sherry Fillmore, Bradley WalkerAbstract:Abstract There has been continued interest in bacteriocins research from an applied perspective as bacteriocins have potential to be used as natural preservative. Four bacteriocinogenic lactic acid bacteria (LAB) strains of Lactobacillus curvatus (Arla-10), Enterococcus faecium (JFR-1), Lactobacillus paracasei subsp. paracasei (JFR-5) and Streptococcus thermophilus (TSB-8) were previously isolated and identified in our lab. The objective of this study was to determine the optimal growth conditions for both LAB growth and bacteriocins production. In this study, various growth conditions including Culture Media (MRS and BHI), initial pH of Culture Media (4.5, 5.5, 6.2, 7.4 and 8.5), and incubation temperatures (20, 37 and 44 °C) were investigated for LAB growth measured as optical density (OD), bacteriocin activity determined as arbitrary unit and viability of LAB expressed as log CFU ml−1. Growth curves of the bacteriocinogenic LAB were generated using a Bioscreen C. Our results indicated that Arla-10, JFR-1, and JFR-5 strains grew well on both MRS and BHI Media at growth temperature tested whereas TSB-8 strain, unable to grow at 20 °C. LAB growth was significantly affected by the initial pH of Culture Media (p
-
Influence of Culture Media, pH and temperature on growth and bacteriocin production of bacteriocinogenic lactic acid bacteria
'Springer Science and Business Media LLC', 2018Co-Authors: En Yang, Fan Lihua, Yan Jinping, Jiang Yueming, Doucette Craig, Fillmore Sherry, Walker BradleyAbstract:There has been continued interest in bacteriocins research from an applied perspective as bacteriocins have potential to be used as natural preservative. Four bacteriocinogenic lactic acid bacteria (LAB) strains of Lactobacillus curvatus (Arla-10), Enterococcus faecium (JFR-1), Lactobacillus paracasei subsp. paracasei (JFR-5) and Streptococcus thermophilus (TSB-8) were previously isolated and identified in our lab. The objective of this study was to determine the optimal growth conditions for both LAB growth and bacteriocins production. In this study, various growth conditions including Culture Media (MRS and BHI), initial pH of Culture Media (4.5, 5.5, 6.2, 7.4 and 8.5), and incubation temperatures (20, 37 and 44 degrees C) were investigated for LAB growth measured as optical density (OD), bacteriocin activity determined as arbitrary unit and viability of LAB expressed as log CFU ml(-1). Growth curves of the bacteriocinogenic LAB were generated using a Bioscreen C. Our results indicated that Arla-10, JFR-1, and JFR-5 strains grew well on both MRS and BHI Media at growth temperature tested whereas TSB-8 strain, unable to grow at 20 degrees C. LAB growth was significantly affected by the initial pH of Culture Media (p < 0.001) and the optimal pH was found ranging from 6.2 to 8.5. Bacteriocin activity was significantly different in MRS versus BHI (p < 0.001), and the optimal condition for LAB to produce bacteriocins was determined in MRS broth, pH 6.2 at 37 degrees C. This study provides useful information on potential application of bacteriocinogenic LAB in food fermentation processes
Marion Bonnet - One of the best experts on this subject based on the ideXlab platform.
-
bacterial Culture through selective and non selective conditions the evolution of Culture Media in clinical microbiology
new microbes and new infections, 2020Co-Authors: Marion BonnetAbstract:Abstract Microbiology has been largely developed thanks to the discovery and optimization of Culture Media. The first liquid artificial Culture medium was created by Louis Pasteur in 1860. Previously, bacterial growth on daily materials such as some foods had been observed. These observations thus highlighted the importance of the bacteria's natural environment and their nutritional needs in the development of Culture Media for their isolation. A Culture medium is essentially composed of basic elements (water, nutrients), to which must be added different growth factors that will be specific to each bacterium and necessary for their growth. The evolution of bacterial Culture through the Media used for their Culture began with the development of the first solid Culture medium by Koch, allowing not only the production of bacterial colonies, but also the possibility of purifying a bacterial clone. The main gelling agent used in solid Culture Media is agar. However, some limits have been observed in the use of agar because of some extremely oxygen sensitive bacteria that do not grow on agar Media and other alternatives were proposed and tested. Then, the discovery of antimicrobial agents and their specific target induced the emergence of selective Media. These inhibiting agents thus make it possible to eliminate undesirable bacteria from microbiota and select the bacteria desired. Thanks to a better knowledge of the bacterial environment, it will be possible to develop new Culture Media and new Culture conditions, better adapted to certain fastidious bacteria, difficult to isolate.
Jean Christophe Lagier - One of the best experts on this subject based on the ideXlab platform.
-
bacterial Culture through selective and non selective conditions the evolution of Culture Media in clinical microbiology
new microbes and new infections, 2020Co-Authors: Marion S Bonnet, Jean Christophe LagierAbstract:Abstract Microbiology has been largely developed thanks to the discovery and optimization of Culture Media. The first liquid artificial Culture medium was created by Louis Pasteur in 1860. Previously, bacterial growth on daily materials such as some foods had been observed. These observations highlighted the importance of the bacteria's natural environment and their nutritional needs in the development of Culture Media for their isolation. A Culture medium is essentially composed of basic elements (water, nutrients), to which must be added different growth factors that will be specific to each bacterium and necessary for their growth. The evolution of bacterial Culture through the Media used for their Culture began with the development of the first solid Culture medium by Koch, allowing not only the production of bacterial colonies, but also the possibility of purifying a bacterial clone. The main gelling agent used in solid Culture Media is agar. However, some limits have been observed in the use of agar because of some extremely oxygen-sensitive bacteria that do not grow on agar Media, and other alternatives were proposed and tested. Then, the discovery of antimicrobial agents and their specific targets prompted the emergence of selective Media. These inhibiting agents make it possible to eliminate undesirable bacteria from the microbiota and select the bacteria desired. Thanks to a better knowledge of the bacterial environment, it will be possible to develop new Culture Media and new Culture conditions, better adapted to certain fastidious bacteria that are difficult to isolate.
Diana Ballhausen - One of the best experts on this subject based on the ideXlab platform.
-
ammonium accumulation and chemokine decrease in Culture Media of gcdh 3d reaggregated brain cell Cultures
Molecular Genetics and Metabolism, 2019Co-Authors: Hongphuc Cudrecung, Noemie Remacle, Sonia Do Valepereira, Mary Gonzalez, Hugues Henry, Julijana Ivanisevic, Jessica Schmiesing, Chris Muhlhausen, Olivier Braissant, Diana BallhausenAbstract:Abstract Glutaric Aciduria type I (GA-I) is caused by mutations in the GCDH gene. Its deficiency results in accumulation of the key metabolites glutaric acid (GA) and 3-hydroxyglutaric acid (3-OHGA) in body tissues and fluids. Present knowledge on the neuropathogenesis of GA-I suggests that GA and 3-OHGA have toxic properties on the developing brain. We analyzed morphological and biochemical features of 3D brain cell aggregates issued from Gcdh−/− mice at two different developmental stages, day-in-vitro (DIV) 8 and 14, corresponding to the neonatal period and early childhood. We also induced a metabolic stress by exposing the aggregates to 10 mM l -lysine (Lys). Significant amounts of GA and 3-OHGA were detected in Gcdh−/− aggregates and their Culture Media. Ammonium was significantly increased in Culture Media of Gcdh−/− aggregates at the early developmental stage. Concentrations of GA, 3-OHGA and ammonium increased significantly after exposure to Lys. Gcdh−/− aggregates manifested morphological alterations of all brain cell types at DIV 8 while at DIV 14 they were only visible after exposure to Lys. Several chemokine levels were significantly decreased in Culture Media of Gcdh−/− aggregates at DIV 14 and after exposure to Lys at DIV 8. This new in vitro model for brain damage in GA-I mimics well in vivo conditions. As seen previously in WT aggregates exposed to 3-OHGA, we confirmed a significant ammonium production by immature Gcdh−/− brain cells. We described for the first time a decrease of chemokines in Gcdh−/− Culture Media which might contribute to brain cell injury in GA-I.
