The Experts below are selected from a list of 231 Experts worldwide ranked by ideXlab platform
Toshifumi Hirata - One of the best experts on this subject based on the ideXlab platform.
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Biotransformation using plant Cultured Cells
Journal of Molecular Catalysis B-enzymatic, 2003Co-Authors: Kohji Ishihara, Toshifumi Hirata, Hiroki Hamada, Nobuyoshi NakajimaAbstract:This review outlines the recent progress during the last 25 years concerning the biotransformation of exogenous substrates by plant Cultured Cells. The plant Cultured Cells have abilities of the regio- and stereoselective hydroxylation, oxido-reduction, hydrogenation, glycosylation, and hydrolysis for various organic compounds as well as microorganisms. The reaction types and the stereochemistry of the products involved in the biotransformations are described. The development of techniques using immobilized plant Cells are also delineated.
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Asymmetric epoxidation of digeranyl by Cultured Cells of Nicotiana tabacum
Journal of Labelled Compounds and Radiopharmaceuticals, 2003Co-Authors: Osamu Nakagawa, Kei Shimoda, Sunsuke Izumi, Toshifumi HirataAbstract:Asymmetric epoxidation of digeranyl, which is a squalene analog, with Cultured Cells of Nicotiana tabacum was investigated. Feeding of [8-3H]-digeranyl into the Cultured Cells of N. tabacum resulted in the formation of (3S)-2,3-epoxydigeranyl and 6,7-epoxydigeranyl. It was found that the epoxidation of digeranyl with N. tabacum was highly stereoselective. Copyright © 2003 John Wiley & Sons, Ltd.
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Enantioselective hydrolyses of α-methylated cyclohexyl acetates by the Cultured Cells of Marchantia polymorpha
Journal of Molecular Catalysis B-enzymatic, 2001Co-Authors: Ryoichi Utsumi, Shunsuke Izumi, Toshifumi HirataAbstract:A high enantioselectivity was observed in the hydrolyses of racemic α-methylated cyclohexyl acetates with the Cultured Cells of Marchantia polymorpha. The enantiomeric excesses of alcohols obtained in the hydrolyses were correlated with the torsional angles between the acetoxyl and α-methyl groups. They have made it possible to predict the optical purity as well as the absolute stereochemistry of alcohols hydrolyzed by using the Cultured Cells of M. polymorpha.
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Enantioselective hydrolysis of 2-methylcyclohexanyl acetates with the Cultured Cells of Marchantia polymorpha
Tetrahedron: Asymmetry, 1993Co-Authors: Toshifumi Hirata, Shunsuke Izumi, Kenji Akita, Hiroaki Yoshida, Shisei GotohAbstract:Abstract Enantioseleclivity in the hydrolysis of trans - and cis -2-methylcyclohexanyl acetates with the Cultured Cells of Marchantia polymorpha was investigated. The Cultured Cells were found to hydrolyze preferentially the acetates having the R -configuration.
Hiroki Hamada - One of the best experts on this subject based on the ideXlab platform.
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Glycosylation of trans-Resveratrol by Plant-Cultured Cells
Bioscience biotechnology and biochemistry, 2012Co-Authors: Hiroya Imai, Kohji Ishihara, Nobuyoshi Nakajima, Megumi Kitagawa, Noriyoshi Masuoka, Kei Shimoda, Hiroki HamadaAbstract:Plant-Cultured Cells of Catharanthus roseus converted trans-resveratrol into its 3-O-β-D-glucopyranoside, 4'-O-β-D-glucopyranoside, 3-O-(6-O-β-D-xylopyranosyl)-β-D-glucopyranoside, and 3-O-(6-O-α-L-arabinopyranosyl)-β-D-glucopyranoside. The 3-O-(6-O-β-D-xylopyranosyl)-β-D-glucopyranoside and 3-O-(6-O-α-L-arabinopyranosyl)-β-D-glucopyranoside compounds of trans-resveratrol are both new. Incubation of plant-Cultured Cells of Ipomoea batatas and Strophanthus gratus with trans-resveratrol gave trans-resveratrol 3-O-β-D-glucopyranoside and trans-resveratrol 4'-O-β-D-glucopyranoside.
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Biotransformation of (+)-catechin by plant Cultured Cells of Eucalyptus perriniana
Plant Biotechnology, 2004Co-Authors: Otani Shuichi, Kohji Ishihara, Hatsuyuki Hamada, Nobuyoshi Nakajima, Tsutomu Furuya, Yoko Kondo, Yoshihisa Asada, Hiroki HamadaAbstract:To examine the biotransformation of (+)-catechin by the plant Cultured Cells of Eucalyptus perriniana, three new biotransformation products, (+)-catechin-3′-O-β-D-glucoside, (+)-catechin-5-O-β-D-glucoside and (+)-catechin-7-O-β-D-glucoside were isolated after 3 days incubation. The Cultured Cells of E. perriniana were found to be capable of glycosylation at the 5, 7-positions on the A ring and the 3′-position on the B ring of (+)-catechin.
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one step glucosylation of capsaicinoids by Cultured Cells of phytolacca americana
Plant Biotechnology, 2003Co-Authors: Hiroki Hamada, Shogo Ohiwa, Tomohisa Nishida, Hisashi Katsuragi, Hatsuyuki Hamada, Takeshi Takeda, Nobuyoshi Nakajima, Kohji IshiharaAbstract:The synthesis of capsaisinoid monoglucoside, a key material for the preparation of the capsaicinoid oligosaccharide, using plant Cultured Cells has been investigated. Among the Cultured Cells tested, only the Cells of Phytolacca americana glucosylated capsaicin and 8-nordihydrocapsaicin into the corresponding monoglucoside in good yields. Thus, the one-step glucosylation of capsaicinoids has been achieved using the Cells of P. americana.
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Biotransformation using plant Cultured Cells
Journal of Molecular Catalysis B-enzymatic, 2003Co-Authors: Kohji Ishihara, Toshifumi Hirata, Hiroki Hamada, Nobuyoshi NakajimaAbstract:This review outlines the recent progress during the last 25 years concerning the biotransformation of exogenous substrates by plant Cultured Cells. The plant Cultured Cells have abilities of the regio- and stereoselective hydroxylation, oxido-reduction, hydrogenation, glycosylation, and hydrolysis for various organic compounds as well as microorganisms. The reaction types and the stereochemistry of the products involved in the biotransformations are described. The development of techniques using immobilized plant Cells are also delineated.
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Biotransformation of (+)- and (−)-camphorquinones by plant Cultured Cells
Phytochemistry, 2001Co-Authors: Wen Chai, Hiroki Hamada, Jumpei Suhara, C. Akira HoriuchiAbstract:Abstract Biotransformation of (+)- and (−)-camphorquinones with suspension plant Cultured Cells of Nicotiana tabacum and Catharanthus roseus was investigated. It was found that the plant Cultured Cells of N. tabacum and C. roseus reduce stereoselectively the carbonyl group of (+)- and (−)-camphorquinones to the corresponding α-keto alcohols.
C. Akira Horiuchi - One of the best experts on this subject based on the ideXlab platform.
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The biocatalytic oxidation of thujopsene by plant Cultured-Cells
Journal of Molecular Catalysis B-enzymatic, 2003Co-Authors: Wen Chai, Yumiko Hayashida, Hiroshi Sakamaki, C. Akira HoriuchiAbstract:The biotransformation of thujopsene (1) using plant suspension Cultured-Cells was investigated. It was found that the Cultured-Cells oxidate thujopsene to 3β-hydroxy-4-thujopsene (2), mayurone (3), and 3β-epoxythujopsan-5β-ol (4). And, it was shown that the biotransformation activity of Cultured-Cells of Hibiscus cannabinus is significantly higher than that of Cultured-Cells of Nicotiana tabacum and Catharanthus roseus.
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Biotransformation of (+)- and (−)-camphorquinones by plant Cultured Cells
Phytochemistry, 2001Co-Authors: Wen Chai, Hiroki Hamada, Jumpei Suhara, C. Akira HoriuchiAbstract:Abstract Biotransformation of (+)- and (−)-camphorquinones with suspension plant Cultured Cells of Nicotiana tabacum and Catharanthus roseus was investigated. It was found that the plant Cultured Cells of N. tabacum and C. roseus reduce stereoselectively the carbonyl group of (+)- and (−)-camphorquinones to the corresponding α-keto alcohols.
Tsutomu Kawasaki - One of the best experts on this subject based on the ideXlab platform.
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Chitin-Triggered MAPK Activation and ROS Generation in Rice Suspension-Cultured Cells.
Methods in molecular biology (Clifton N.J.), 2017Co-Authors: Koji Yamaguchi, Tsutomu KawasakiAbstract:Suspension-Cultured Cells respond sensitively to a number of stimuli including pathogen-derived molecules. Therefore, they are used in a simple, single-cell model system in order to gain insights into immune signaling pathways in rice. Here we describe protocols for studying chitin-induced MAPK activation and ROS generation in rice suspension-Cultured Cells.
Wen Chai - One of the best experts on this subject based on the ideXlab platform.
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The biocatalytic oxidation of thujopsene by plant Cultured-Cells
Journal of Molecular Catalysis B-enzymatic, 2003Co-Authors: Wen Chai, Yumiko Hayashida, Hiroshi Sakamaki, C. Akira HoriuchiAbstract:The biotransformation of thujopsene (1) using plant suspension Cultured-Cells was investigated. It was found that the Cultured-Cells oxidate thujopsene to 3β-hydroxy-4-thujopsene (2), mayurone (3), and 3β-epoxythujopsan-5β-ol (4). And, it was shown that the biotransformation activity of Cultured-Cells of Hibiscus cannabinus is significantly higher than that of Cultured-Cells of Nicotiana tabacum and Catharanthus roseus.
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Biotransformation of (+)- and (−)-camphorquinones by plant Cultured Cells
Phytochemistry, 2001Co-Authors: Wen Chai, Hiroki Hamada, Jumpei Suhara, C. Akira HoriuchiAbstract:Abstract Biotransformation of (+)- and (−)-camphorquinones with suspension plant Cultured Cells of Nicotiana tabacum and Catharanthus roseus was investigated. It was found that the plant Cultured Cells of N. tabacum and C. roseus reduce stereoselectively the carbonyl group of (+)- and (−)-camphorquinones to the corresponding α-keto alcohols.
