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María Angeles Pedreño - One of the best experts on this subject based on the ideXlab platform.
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Critical parameters on which the production of trans -resveratrol in Vitis vinifera cv Monastrell cell cultures depends
Plant Cell Tissue and Organ Culture (PCTOC), 2019Co-Authors: Sarai Belchí-navarro, María Angeles Pedreño, Lorena AlmagroAbstract:The effect of several parameters on Trans-Resveratrol extracellular production in Vitis vinifera cv Monastrell suspension cultured cells elicited with cyclodextrins separately or in combination with methyl jasmonate was evaluated. The results indicated that Trans-Resveratrol production was independent of aeration surface when the same volume of suspension cultured cells was cultivated in flasks of different size (250, 500 and 1000 mL). Moreover, the effect of scale-up on Trans-Resveratrol production under elicitation was also evaluated. The highest Trans-Resveratrol production (2140 mg/L) was observed when 200 mL of suspension cultured cells were cultivated in flasks of 500 mL capacity in the presence of cyclodextrins and methyl jasmonate. Finally, the results also indicated that the same suspension cultured cells can be used for at least six continuous elicitation cycles with cyclodextrins and methyl jasmonate to enhance the production of Trans-Resveratrol. Trans-Resveratrol production was independent of aeration surface and the same grapevine cells could be used for at least six continuous elicitation cycles to enhance the production of Trans-Resveratrol.
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Enhanced extracellular production of Trans-Resveratrol in Vitis vinifera suspension cultured cells by using cyclodextrins and coronatine
Plant Physiology and Biochemistry, 2015Co-Authors: Lorena Almagro, Sarai Belchí-navarro, Ascensión Martínez-márquez, María Angeles PedreñoAbstract:Abstract In the present work the effect of cyclodextrin and coronatine on both trans -resveratrol production and the expression of stilbene biosynthetic genes in Vitis vinifera L. cv Monastrell suspension cultured cells were evaluated. The results showed the maximum level of trans -resveratrol produced by cells and secreted to the culture medium with 50 mM cyclodextrins and 1 μM coronatine. Since the levels of trans -resveratrol produced in the combined treatment were higher than the sum of the individual treatments, a synergistic effect between both elicitors was assumed. In addition, all the analysed genes were induced by cyclodextrins and/or coronatine. The expression of the phenylalanine ammonia lyase and stilbene synthase genes was greatly enhanced by coronatine although an increase in the amount of trans -resveratrol in the spent medium was not detected. Therefore, despite the fact that trans -resveratrol production is related with the expression of genes involved in the biosynthetic process, other factors may be involved, such as post-transcriptional and post-traductional regulation. The expression maximal levels of cinnamate 4-hydroxylase and 4-coumarate-CoA ligase genes were found with cyclodextrins alone or in combination with coronatine suggesting that the activity of these enzymes could be not only important for the formation of intermediates of trans -R biosynthesis but also for those intermediates involved in the biosynthesis of lignins and/or flavonoids.
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Enhanced extracellular production of Trans-Resveratrol in Vitis vinifera suspension cultured cells by using cyclodextrins and methyljasmonate.
Plant cell reports, 2011Co-Authors: Sarai Belchí-navarro, Lorena Almagro, Diego Lijavetzky, Roque Bru, María Angeles PedreñoAbstract:In this work, the effect of different inducing factors on Trans-Resveratrol extracellular production in Monastrell grapevine suspension cultured cells is evaluated. A detailed analysis provides the optimal concentrations of cyclodextrins, methyljasmonate and UV irradiation dosage, optimal cell density, elicitation time and sucrose content in the culture media. The results indicate that Trans-Resveratrol production decreases as the initial cell density increases for a constant elicitor concentration in Monastrell suspension cultured cells treated with cyclodextrins individually or in combination with methyljasmonate; the decrease observed in cell cultures elicited with cyclodextrins alone is far more drastic than those observed in the combined treatment. Trans-Resveratrol extracellular production observed by the joint use of cyclodextrins and methyljasmonate (1,447.8 ± 60.4 μmol Trans-Resveratrol g(-1) dry weight) is lower when these chemical compounds are combined with UV light short exposure (669.9 ± 45.2 μmol Trans-Resveratrol g(-1) dry weight). Likewise, Trans-Resveratrol production is dependent on levels of sucrose in the elicitation medium with the maximal levels observed with 20 g l(-1) sucrose and the joint action of cyclodextrins and 100 μM methyljasmonate. The sucrose concentration did not seem to limit the process although it affects significantly the specific productivity since the lowest sucrose concentration is 10 g l(-1), the highest productivity is reached (100.7 ± 5.8 μmol Trans-Resveratrol g(-1) dry weight g(-1) sucrose) using cyclodextrins and 25 μM methyljasmonate.
Lorena Almagro - One of the best experts on this subject based on the ideXlab platform.
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Critical parameters on which the production of trans -resveratrol in Vitis vinifera cv Monastrell cell cultures depends
Plant Cell Tissue and Organ Culture (PCTOC), 2019Co-Authors: Sarai Belchí-navarro, María Angeles Pedreño, Lorena AlmagroAbstract:The effect of several parameters on Trans-Resveratrol extracellular production in Vitis vinifera cv Monastrell suspension cultured cells elicited with cyclodextrins separately or in combination with methyl jasmonate was evaluated. The results indicated that Trans-Resveratrol production was independent of aeration surface when the same volume of suspension cultured cells was cultivated in flasks of different size (250, 500 and 1000 mL). Moreover, the effect of scale-up on Trans-Resveratrol production under elicitation was also evaluated. The highest Trans-Resveratrol production (2140 mg/L) was observed when 200 mL of suspension cultured cells were cultivated in flasks of 500 mL capacity in the presence of cyclodextrins and methyl jasmonate. Finally, the results also indicated that the same suspension cultured cells can be used for at least six continuous elicitation cycles with cyclodextrins and methyl jasmonate to enhance the production of Trans-Resveratrol. Trans-Resveratrol production was independent of aeration surface and the same grapevine cells could be used for at least six continuous elicitation cycles to enhance the production of Trans-Resveratrol.
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Enhanced extracellular production of Trans-Resveratrol in Vitis vinifera suspension cultured cells by using cyclodextrins and coronatine
Plant Physiology and Biochemistry, 2015Co-Authors: Lorena Almagro, Sarai Belchí-navarro, Ascensión Martínez-márquez, María Angeles PedreñoAbstract:Abstract In the present work the effect of cyclodextrin and coronatine on both trans -resveratrol production and the expression of stilbene biosynthetic genes in Vitis vinifera L. cv Monastrell suspension cultured cells were evaluated. The results showed the maximum level of trans -resveratrol produced by cells and secreted to the culture medium with 50 mM cyclodextrins and 1 μM coronatine. Since the levels of trans -resveratrol produced in the combined treatment were higher than the sum of the individual treatments, a synergistic effect between both elicitors was assumed. In addition, all the analysed genes were induced by cyclodextrins and/or coronatine. The expression of the phenylalanine ammonia lyase and stilbene synthase genes was greatly enhanced by coronatine although an increase in the amount of trans -resveratrol in the spent medium was not detected. Therefore, despite the fact that trans -resveratrol production is related with the expression of genes involved in the biosynthetic process, other factors may be involved, such as post-transcriptional and post-traductional regulation. The expression maximal levels of cinnamate 4-hydroxylase and 4-coumarate-CoA ligase genes were found with cyclodextrins alone or in combination with coronatine suggesting that the activity of these enzymes could be not only important for the formation of intermediates of trans -R biosynthesis but also for those intermediates involved in the biosynthesis of lignins and/or flavonoids.
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Enhanced extracellular production of Trans-Resveratrol in Vitis vinifera suspension cultured cells by using cyclodextrins and methyljasmonate.
Plant cell reports, 2011Co-Authors: Sarai Belchí-navarro, Lorena Almagro, Diego Lijavetzky, Roque Bru, María Angeles PedreñoAbstract:In this work, the effect of different inducing factors on Trans-Resveratrol extracellular production in Monastrell grapevine suspension cultured cells is evaluated. A detailed analysis provides the optimal concentrations of cyclodextrins, methyljasmonate and UV irradiation dosage, optimal cell density, elicitation time and sucrose content in the culture media. The results indicate that Trans-Resveratrol production decreases as the initial cell density increases for a constant elicitor concentration in Monastrell suspension cultured cells treated with cyclodextrins individually or in combination with methyljasmonate; the decrease observed in cell cultures elicited with cyclodextrins alone is far more drastic than those observed in the combined treatment. Trans-Resveratrol extracellular production observed by the joint use of cyclodextrins and methyljasmonate (1,447.8 ± 60.4 μmol Trans-Resveratrol g(-1) dry weight) is lower when these chemical compounds are combined with UV light short exposure (669.9 ± 45.2 μmol Trans-Resveratrol g(-1) dry weight). Likewise, Trans-Resveratrol production is dependent on levels of sucrose in the elicitation medium with the maximal levels observed with 20 g l(-1) sucrose and the joint action of cyclodextrins and 100 μM methyljasmonate. The sucrose concentration did not seem to limit the process although it affects significantly the specific productivity since the lowest sucrose concentration is 10 g l(-1), the highest productivity is reached (100.7 ± 5.8 μmol Trans-Resveratrol g(-1) dry weight g(-1) sucrose) using cyclodextrins and 25 μM methyljasmonate.
Sarai Belchí-navarro - One of the best experts on this subject based on the ideXlab platform.
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Critical parameters on which the production of trans -resveratrol in Vitis vinifera cv Monastrell cell cultures depends
Plant Cell Tissue and Organ Culture (PCTOC), 2019Co-Authors: Sarai Belchí-navarro, María Angeles Pedreño, Lorena AlmagroAbstract:The effect of several parameters on Trans-Resveratrol extracellular production in Vitis vinifera cv Monastrell suspension cultured cells elicited with cyclodextrins separately or in combination with methyl jasmonate was evaluated. The results indicated that Trans-Resveratrol production was independent of aeration surface when the same volume of suspension cultured cells was cultivated in flasks of different size (250, 500 and 1000 mL). Moreover, the effect of scale-up on Trans-Resveratrol production under elicitation was also evaluated. The highest Trans-Resveratrol production (2140 mg/L) was observed when 200 mL of suspension cultured cells were cultivated in flasks of 500 mL capacity in the presence of cyclodextrins and methyl jasmonate. Finally, the results also indicated that the same suspension cultured cells can be used for at least six continuous elicitation cycles with cyclodextrins and methyl jasmonate to enhance the production of Trans-Resveratrol. Trans-Resveratrol production was independent of aeration surface and the same grapevine cells could be used for at least six continuous elicitation cycles to enhance the production of Trans-Resveratrol.
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Enhanced extracellular production of Trans-Resveratrol in Vitis vinifera suspension cultured cells by using cyclodextrins and coronatine
Plant Physiology and Biochemistry, 2015Co-Authors: Lorena Almagro, Sarai Belchí-navarro, Ascensión Martínez-márquez, María Angeles PedreñoAbstract:Abstract In the present work the effect of cyclodextrin and coronatine on both trans -resveratrol production and the expression of stilbene biosynthetic genes in Vitis vinifera L. cv Monastrell suspension cultured cells were evaluated. The results showed the maximum level of trans -resveratrol produced by cells and secreted to the culture medium with 50 mM cyclodextrins and 1 μM coronatine. Since the levels of trans -resveratrol produced in the combined treatment were higher than the sum of the individual treatments, a synergistic effect between both elicitors was assumed. In addition, all the analysed genes were induced by cyclodextrins and/or coronatine. The expression of the phenylalanine ammonia lyase and stilbene synthase genes was greatly enhanced by coronatine although an increase in the amount of trans -resveratrol in the spent medium was not detected. Therefore, despite the fact that trans -resveratrol production is related with the expression of genes involved in the biosynthetic process, other factors may be involved, such as post-transcriptional and post-traductional regulation. The expression maximal levels of cinnamate 4-hydroxylase and 4-coumarate-CoA ligase genes were found with cyclodextrins alone or in combination with coronatine suggesting that the activity of these enzymes could be not only important for the formation of intermediates of trans -R biosynthesis but also for those intermediates involved in the biosynthesis of lignins and/or flavonoids.
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Enhanced extracellular production of Trans-Resveratrol in Vitis vinifera suspension cultured cells by using cyclodextrins and methyljasmonate.
Plant cell reports, 2011Co-Authors: Sarai Belchí-navarro, Lorena Almagro, Diego Lijavetzky, Roque Bru, María Angeles PedreñoAbstract:In this work, the effect of different inducing factors on Trans-Resveratrol extracellular production in Monastrell grapevine suspension cultured cells is evaluated. A detailed analysis provides the optimal concentrations of cyclodextrins, methyljasmonate and UV irradiation dosage, optimal cell density, elicitation time and sucrose content in the culture media. The results indicate that Trans-Resveratrol production decreases as the initial cell density increases for a constant elicitor concentration in Monastrell suspension cultured cells treated with cyclodextrins individually or in combination with methyljasmonate; the decrease observed in cell cultures elicited with cyclodextrins alone is far more drastic than those observed in the combined treatment. Trans-Resveratrol extracellular production observed by the joint use of cyclodextrins and methyljasmonate (1,447.8 ± 60.4 μmol Trans-Resveratrol g(-1) dry weight) is lower when these chemical compounds are combined with UV light short exposure (669.9 ± 45.2 μmol Trans-Resveratrol g(-1) dry weight). Likewise, Trans-Resveratrol production is dependent on levels of sucrose in the elicitation medium with the maximal levels observed with 20 g l(-1) sucrose and the joint action of cyclodextrins and 100 μM methyljasmonate. The sucrose concentration did not seem to limit the process although it affects significantly the specific productivity since the lowest sucrose concentration is 10 g l(-1), the highest productivity is reached (100.7 ± 5.8 μmol Trans-Resveratrol g(-1) dry weight g(-1) sucrose) using cyclodextrins and 25 μM methyljasmonate.
Joana M. Planas - One of the best experts on this subject based on the ideXlab platform.
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Colorectal cancer chemoprevention by Trans-Resveratrol.
Pharmacological research, 2012Co-Authors: M. Emília Juan, Irene Alfaras, Joana M. PlanasAbstract:Trans-Resveratrol (trans-3,4',5-trihydroxystilbene) is a natural phytoalexin present in grapes, red wine, berries and peanuts with health protecting properties. The low oral bioavailability indicated for this polyphenol, with the intestine as a bottleneck to its absorption, has promoted the large intestine as a potential target site for its chemopreventive activity. This review recapitulates the current evidence of the effects of Trans-Resveratrol on colon cancer. First, we describe the studies conducted in vitro which show that the protective activity takes place by inhibition of proliferation and induction of apoptosis. Secondly, the chemopreventive activity in animal models of colon carcinogenesis is revised. Trans-Resveratrol not only reduces the number of preneoplastic lesions but also the incidence and multiplicity of tumors. Lastly, the article also reviews the available data on clinical trials. Altogether, the present findings support the hypothesis that the oral administration of Trans-Resveratrol might contribute to the prevention of colon carcinogenesis.
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Trans-Resveratrol Reduces Precancerous Colonic Lesions in Dimethylhydrazine-Treated Rats
Journal of agricultural and food chemistry, 2010Co-Authors: Irene Alfaras, M. Emília Juan, Joana M. PlanasAbstract:Trans-Resveratrol, a natural occurring polyphenol, has been described as an antiproliferative and proapoptotic agent in vitro. Here, we studied the effect of Trans-Resveratrol administered orally at a dose of 60 mg/kg for 49 days on early preneoplastic markers induced by the intraperitoneal injection of 1,2-dimethylhydrazine (20 mg/kg). We measured Trans-Resveratrol and its derivates by liquid-liquid extraction followed by high-performance liquid chromatography diode array detection analysis in colon contents. Dihydroresveratrol was the most abundant compound in the colon, followed by Trans-Resveratrol glucuronide and small amounts of Trans-Resveratrol and its sulfate. The administration of Trans-Resveratrol decreased aberrant crypt foci by 52%, and mucin depleted foci by 45% in colon. In conclusion, the correlation between the reduction of precancerous colonic lesions and the availability of Trans-Resveratrol in the colon provides a new insight into the therapeutical potential of this polyphenol and its metabolites.
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Quantification of Trans-Resveratrol and its metabolites in rat plasma and tissues by HPLC.
Journal of pharmaceutical and biomedical analysis, 2009Co-Authors: M. Emília Juan, Mònica Maijó, Joana M. PlanasAbstract:Trans-Resveratrol, a polyphenol from grapes, is being recognized as a bioactive agent with potential beneficial effects on health. However, little is known about its distribution in the organism mainly because of the lack of accurate and precise detection methods. Consequently the aim of the present study was to develop a methodology of extraction and quantification of Trans-Resveratrol and its metabolites in plasma, brain, testis, liver, lungs and kidney by HPLC. To this end, the time of homogenization and liquid extraction were adapted to the different tissues. The methods were validated using homogenized tissues spiked with pure Trans-Resveratrol. The precision (% R.S.D.) ranged from 3.7% in testis to 13.2% in lungs. Recoveries were 98.5+/-3.2% (liver), 100.1+/-1.8% (kidney), 96.5+/-7.6% (lungs), 99.0+/-0.7% (brain) and 103+/-2.7% (testicle). The limits of detection ranged from 5.5 nM in testis to 11.2 nM in kidney. After validation, the methods were applied to the assessment of the bioavailability and distribution of Trans-Resveratrol in rats after the intravenous administration of 15 mg/kg. At 90 min, Trans-Resveratrol and its glucuronide and sulfate conjugates were widely distributed in all the tissues studied. The highest concentrations (nmol/g tissue) were found in kidney (resveratrol: 1.45+/-0.35; glucuronide: 2.91+/-0.19; sulfate: not detected), and the lowest in brain (resveratrol: 0.17+/-0.04; glucuronide: not detected; sulfate: 0.04+/-0.01). In conclusion, accurate and reproducible methods have been described to identify target tissues of resveratrol as a first step to understand its mechanisms of action in vivo.
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The Daily Oral Administration of High Doses of Trans-Resveratrol to Rats for 28 Days Is Not Harmful
The Journal of nutrition, 2002Co-Authors: M. Emília Juan, M. Pilar Vinardell, Joana M. PlanasAbstract:trans-3,5,4'-Trihydroxystilbene (Trans-Resveratrol) is a phytochemical present in peanuts, grapes and wine with beneficial effects such as protection against cardiovascular disease and cancer prevention. The purpose of this study was to evaluate whether high doses of Trans-Resveratrol have harmful effects on Sprague-Dawley rats. Trans-Resveratrol was administered orally to male rats for 28 d at a dose of 20 mg/(kg x d), 1000 times the amount consumed by a 70-kg person taking 1.4 g of Trans-Resveratrol/d. Body weight, and food and water consumption did not differ between rats treated with Trans-Resveratrol and the control group. Hematologic and biochemical variables were not affected by the treatment. Histopathologic examination of the organs obtained at autopsy did not reveal any alterations. These results support the view that repeated consumption of Trans-Resveratrol at 20 mg/(kg x d) does not adversely affect the variables tested in rats.
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Determination of Trans-Resveratrol in plasma by HPLC.
Analytical chemistry, 1999Co-Authors: M E Juan, Rosa M. Lamuela-raventós, M. C. De La Torre-boronat, Joana M. PlanasAbstract:Trans-Resveratrol (trans-3,5,4'-trihydroxystilbene), a phenolic compound present in grapes, wines, and peanuts, has been reported to have health benefits including anticarcinogenic effects and protection against cardiovascular diseases. Despite its importance, little is known about its bioavailability in both humans and animals. A fundamental step for this evaluation consisted in measuring this stilbene in blood. In the present work, a simple and rapid HPLC method with diode array-UV detection has been developed. Resveratrol contained in plasma was purified by solid-phase extraction using a C18 cartridge. The sample was rinsed with water and methanol-water (25:75 v/v), and Trans-Resveratrol was finally eluted with methanol. The collected fraction was evaporated under nitrogen and analyzed by HPLC. The method was validated by obtaining a linear correlation, a detection limit of 20 micrograms/L, and a good precision with a coefficient of variation of 2.85%. Trans-Resveratrol administered orally to rats was detected in plasma. With this procedure, excellent separation of Trans-Resveratrol is achieved, thus allowing a rapid analysis of the sample for absorption, distribution, and metabolism studies.
Christophoros Zonas - One of the best experts on this subject based on the ideXlab platform.
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Trans -Resveratrol Concentration in Wines Produced in Greece
Journal of Food Composition and Analysis, 1999Co-Authors: Vassilis Dourtoglou, Dimitrios P. Makris, Fabienne Bois-dounas, Christophoros ZonasAbstract:Abstract A direct injection-HPLC method, based on existing methods, slightly modified, was used to analyse commercially available wines in the Greek market for their trans -resveratrol content. Red wines produced from French, or French and Greek cultivars were found to have relatively higher amounts of trans -resveratrol compared with those produced exclusively from native Vitis vinifera cultivars. However, the wines produced from the original Greek Vitis vinifera cultivars, Kotsifali and Mantilaria , appeared to contain important amounts of trans -resveratrol. White wines were found to have markedly lower concentrations of this stilbene in comparison with red wines.
