The Experts below are selected from a list of 5319 Experts worldwide ranked by ideXlab platform
Gouranga Biswas - One of the best experts on this subject based on the ideXlab platform.
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Immune responses to methanolic extract of black Cumin (Nigella sativa) in rainbow trout (Oncorhynchus mykiss).
Fish & shellfish immunology, 2017Co-Authors: Yasemin Celik Altunoglu, Soner Bilen, Ferhat Ulu, Gouranga BiswasAbstract:Abstract The immune stimulating effects of the methanolic extract of black Cumin (Nigella sativa) in rainbow trout (Oncorhynchus mykiss) was evaluated. Variable concentrations of black Cumin methanolic extract [0 (Control), 0.1 and 0.5 g kg−1 of feed] were individually added to the basal diet and rainbow trout was fed for 30 days to assess the innate immune responses and growth performance. Feed conversion ratio significantly decreased in the group fed with 0.5 g kg−1 black Cumin extract. Respiratory burst activity was observed to be the highest in the 0.5 g kg−1 black Cumin extract fed group. Lysozyme and myeloperoxidase activities were significantly increased in fish of experimental groups compared to control (P 0.05) after challenged with Aeromonas hydrophila. The results indicate that the methanolic extract of black Cumin is a stimulator of some innate humoral immune responses, but it is ineffective for cytokine-related gene trancriptions in rainbow trout.
Uzma Zaman - One of the best experts on this subject based on the ideXlab platform.
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protein profiling of non model plant Cuminum cyminum by gel based proteomic approach
Phytochemical Analysis, 2018Co-Authors: Uzma Zaman, Henning Urlaub, Atiya AbbasiAbstract:Introduction Cumin (Cuminum cyminum), a popular spice has been widely used in traditional medicine to cure various ailments. Despite the existence of scientific literature about its pharmacological properties, no successful proteome profiling has yet been attempted. Objective To optimise extraction of Cumin proteins and analyse its profile by shotgun proteomics, using one-dimensional electrophoresis coupled with nano-ESI-LC–MS/MS. Methodology As a first step, we have compared three extraction protocols for total proteins extraction from Cumin. Extracted proteins were separated on one-dimensional gel and analysed by state-of-the-art linear ion trap (LTQ)-Orbitrap Velose and Q Exactive HF mass spectrometer. Results Evaluation of extraction method revealed significant differences in protein yield and proteome composition between the three extracts. LC–MS/MS allowed identification of several proteins with functional significance in various biological processes. Conclusion This study provides identification of a large number of proteins and offers a molecular basis for future research on potential pharmacologically active Cumin proteins. Copyright © 2017 John Wiley & Sons, Ltd.
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isolation purification and characterization of a nonspecific lipid transfer protein from Cuminum cyminum
Phytochemistry, 2009Co-Authors: Uzma Zaman, Atiya AbbasiAbstract:Abstract Cuminum cyminum, an aromatic plant from the family Umbelliferae, is used as a flavoring and seasoning agent in foods. This communication reports the characterization of a nonspecific lipid transfer protein nsLTP1 from its seeds. Plant nsLTPs are small basic proteins involved in transport of lipids between membranes. These proteins are known to participate in plant defense; however, the exact mechanism of their antimicrobial action against fungi or bacteria is still unclear. The Cumin nsLTP1 has been purified using a combination of chromatographic procedures and further characterized using mass spectrometry, circular dichroism spectroscopy and Edman degradation. Amino acid sequence has been used to predict homology model of Cumin nsLTP1 in complex with myristic acid, and lyso-myristoyl phosphatidyl choline (LMPC). Cumin nsLTP1 is a monomeric protein with a molecular weight of 9.7 kDa as estimated by SDS–PAGE and ESIMS. The protein shows an isoelectric point of 7.8 on 6% PAGE. The primary structure consists of 92 amino acids with eight conserved cysteine residues. The global fold of Cumin nsLTP1 includes four α-helices stabilized by four disulfide bonds and a C-terminal tail. The role of internal hydrophobic cavity of the protein in lipid transfer is discussed.
Atiya Abbasi - One of the best experts on this subject based on the ideXlab platform.
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protein profiling of non model plant Cuminum cyminum by gel based proteomic approach
Phytochemical Analysis, 2018Co-Authors: Uzma Zaman, Henning Urlaub, Atiya AbbasiAbstract:Introduction Cumin (Cuminum cyminum), a popular spice has been widely used in traditional medicine to cure various ailments. Despite the existence of scientific literature about its pharmacological properties, no successful proteome profiling has yet been attempted. Objective To optimise extraction of Cumin proteins and analyse its profile by shotgun proteomics, using one-dimensional electrophoresis coupled with nano-ESI-LC–MS/MS. Methodology As a first step, we have compared three extraction protocols for total proteins extraction from Cumin. Extracted proteins were separated on one-dimensional gel and analysed by state-of-the-art linear ion trap (LTQ)-Orbitrap Velose and Q Exactive HF mass spectrometer. Results Evaluation of extraction method revealed significant differences in protein yield and proteome composition between the three extracts. LC–MS/MS allowed identification of several proteins with functional significance in various biological processes. Conclusion This study provides identification of a large number of proteins and offers a molecular basis for future research on potential pharmacologically active Cumin proteins. Copyright © 2017 John Wiley & Sons, Ltd.
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isolation purification and characterization of a nonspecific lipid transfer protein from Cuminum cyminum
Phytochemistry, 2009Co-Authors: Uzma Zaman, Atiya AbbasiAbstract:Abstract Cuminum cyminum, an aromatic plant from the family Umbelliferae, is used as a flavoring and seasoning agent in foods. This communication reports the characterization of a nonspecific lipid transfer protein nsLTP1 from its seeds. Plant nsLTPs are small basic proteins involved in transport of lipids between membranes. These proteins are known to participate in plant defense; however, the exact mechanism of their antimicrobial action against fungi or bacteria is still unclear. The Cumin nsLTP1 has been purified using a combination of chromatographic procedures and further characterized using mass spectrometry, circular dichroism spectroscopy and Edman degradation. Amino acid sequence has been used to predict homology model of Cumin nsLTP1 in complex with myristic acid, and lyso-myristoyl phosphatidyl choline (LMPC). Cumin nsLTP1 is a monomeric protein with a molecular weight of 9.7 kDa as estimated by SDS–PAGE and ESIMS. The protein shows an isoelectric point of 7.8 on 6% PAGE. The primary structure consists of 92 amino acids with eight conserved cysteine residues. The global fold of Cumin nsLTP1 includes four α-helices stabilized by four disulfide bonds and a C-terminal tail. The role of internal hydrophobic cavity of the protein in lipid transfer is discussed.
Yasemin Celik Altunoglu - One of the best experts on this subject based on the ideXlab platform.
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Immune responses to methanolic extract of black Cumin (Nigella sativa) in rainbow trout (Oncorhynchus mykiss).
Fish & shellfish immunology, 2017Co-Authors: Yasemin Celik Altunoglu, Soner Bilen, Ferhat Ulu, Gouranga BiswasAbstract:Abstract The immune stimulating effects of the methanolic extract of black Cumin (Nigella sativa) in rainbow trout (Oncorhynchus mykiss) was evaluated. Variable concentrations of black Cumin methanolic extract [0 (Control), 0.1 and 0.5 g kg−1 of feed] were individually added to the basal diet and rainbow trout was fed for 30 days to assess the innate immune responses and growth performance. Feed conversion ratio significantly decreased in the group fed with 0.5 g kg−1 black Cumin extract. Respiratory burst activity was observed to be the highest in the 0.5 g kg−1 black Cumin extract fed group. Lysozyme and myeloperoxidase activities were significantly increased in fish of experimental groups compared to control (P 0.05) after challenged with Aeromonas hydrophila. The results indicate that the methanolic extract of black Cumin is a stimulator of some innate humoral immune responses, but it is ineffective for cytokine-related gene trancriptions in rainbow trout.
Christine Baly - One of the best experts on this subject based on the ideXlab platform.
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Artificial milk preference of newborn lambs is prenatally influenced by transfer of the flavor from the maternal diet to the amniotic fluid.
Physiology & behavior, 2020Co-Authors: Frédéric Lévy, Karine Badonnel, Aline Bertin, F. Cornilleau, Didier Durieux, M. Meurisse, Raymond Nowak, Céline Parias, Marie-annick Persuy, Christine BalyAbstract:The present study examined in lambs whether exposure to flavors derived from pregnant mother's diet and transferred to amniotic fluid (AF) could induce a preference for artificial milk containing one of these flavors. To test this hypothesis, Cumin was added to the maternal diet in the last month of gestation. Preference for artificial milk containing p-cymene, one of the chemosensory compounds of Cumin, was tested within the first two days after birth in maternally deprived lambs born from mothers fed a Cumin-flavored diet (Cumin group), or an unflavored diet (Control group). Aromatic profile of AF from Cumin-fed mothers was analyzed by GC-MS/MS to determine whether p-cymene could be detected. While the control group avoided the flavored artificial milk on day 1, the Cumin group did not and showed a preference for the Cumin-scented formula on day 2. GC-MS/MS profile of AF revealed that four of the main volatile Cumin compounds, p-cymene, p-cymenene, β-pinene and γ-terpinene were present in variable amounts in all samples, p-cymene being the most frequently detected. These findings indicate that newborn lambs can memorize flavors from the mother's diet present in AF and that prenatal experience influences their preference for an artificial milk containing one specific flavor.
