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Jonathan Cherng - One of the best experts on this subject based on the ideXlab platform.
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discovery of a novel anti cancer agent targeting both topoisomerase i ii as well as telomerase activities in human lung adenocarcinoma a549 cells in vitro and in vivo cinnamomum verum component Cuminaldehyde
Current Cancer Drug Targets, 2016Co-Authors: Tawei Chen, Shumei Yang, Hoyiu Wong, Kuoshen Chou, Jonathan Cherng, Kuendaw Tsai, Yi-heng Liu, Yang-tz Wang, Janise Cuizon, Jaw-ming CherngAbstract:Cinnamomum verum is used to make the spice cinnamon and has been used for more than 5000 years by both of the two most ancient forms of medicine in the words: Ayurveda and traditional Chinese herbal medicines for various applications such as adenopathy, rheumatism, dermatosis, dyspepsia, stroke, tumors, elephantiasis, trichomonas, yeast, and virus infections. We evaluated the anticancer effect of Cuminaldehyde (CuA), a constituent of the bark of the plant, and its underlying molecular biomarkers associated with carcinogenesis in human lung adenocarcinoma A549 cells. The results show that Cuminaldehyde suppressed proliferation and induced apoptosis as indicated by mitochondrial membrane potential loss, activation of caspase 3 and 9, increase in annexin V+PI+ cells, and morphological characteristics of apoptosis, including blebbing of plasma membrane, nuclear condensation, fragmentation, apoptotic body formation, and comet with elevated tail intensity and moment. In addition, Cuminaldehyde also induced lysosomal vacuolation with increased volume of acidic compartments (VAC), suppressions of both topoisomerase I & II as well as telomerase activities in a dose-dependent manner. Further study reveals the growth-inhibitory effect of Cuminaldehyde was also evident in a nude mice model. Taken together, the data suggest that the growth-inhibitory effect of Cuminaldehyde against A549 cells is accompanied by downregulations of proliferative control involving apoptosis, both topoisomerase I & II as well as telomerase activities, together with an upregulation of lysosomal vacuolation and VAC. Similar effects (including all of the above-mentioned effects) were found in other cell lines, including human lung squamous cell carcinoma NCI-H520 and colorectal adenocarcinoma COLO 205 (results not shown). Our data suggest that Cuminaldehyde could be a potential agent for anticancer therapy.
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Discovery of a Novel Anti-Cancer Agent Targeting Both Topoisomerase I & II as Well as Telomerase Activities in Human Lung Adenocarcinoma A549 Cells In Vitro and In Vivo: Cinnamomum verum Component Cuminaldehyde
Current cancer drug targets, 2016Co-Authors: Tawei Chen, Shumei Yang, Hoyiu Wong, Kuoshen Chou, Jonathan Cherng, Kuendaw Tsai, Yi-heng Liu, Yang-tz Wang, Janise Cuizon, Jaw-ming CherngAbstract:Cinnamomum verum is used to make the spice cinnamon and has been used for more than 5000 years by both of the two most ancient forms of medicine in the words: Ayurveda and traditional Chinese herbal medicines for various applications such as adenopathy, rheumatism, dermatosis, dyspepsia, stroke, tumors, elephantiasis, trichomonas, yeast, and virus infections. We evaluated the anticancer effect of Cuminaldehyde (CuA), a constituent of the bark of the plant, and its underlying molecular biomarkers associated with carcinogenesis in human lung adenocarcinoma A549 cells. The results show that Cuminaldehyde suppressed proliferation and induced apoptosis as indicated by mitochondrial membrane potential loss, activation of caspase 3 and 9, increase in annexin V+PI+ cells, and morphological characteristics of apoptosis, including blebbing of plasma membrane, nuclear condensation, fragmentation, apoptotic body formation, and comet with elevated tail intensity and moment. In addition, Cuminaldehyde also induced lysosomal vacuolation with increased volume of acidic compartments (VAC), suppressions of both topoisomerase I & II as well as telomerase activities in a dose-dependent manner. Further study reveals the growth-inhibitory effect of Cuminaldehyde was also evident in a nude mice model. Taken together, the data suggest that the growth-inhibitory effect of Cuminaldehyde against A549 cells is accompanied by downregulations of proliferative control involving apoptosis, both topoisomerase I & II as well as telomerase activities, together with an upregulation of lysosomal vacuolation and VAC. Similar effects (including all of the above-mentioned effects) were found in other cell lines, including human lung squamous cell carcinoma NCI-H520 and colorectal adenocarcinoma COLO 205 (results not shown). Our data suggest that Cuminaldehyde could be a potential agent for anticancer therapy.
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Cuminaldehyde from cinnamomum verum induces cell death through targeting topoisomerase 1 and 2 in human colorectal adenocarcinoma colo 205 cells
Nutrients, 2016Co-Authors: Kuendaw Tsai, Shumei Yang, Hoyiu Wong, Tawei Chen, Kuoshen Chou, Jonathan CherngAbstract:Cinnamomum verum, also called true cinnamon tree, is employed to make the seasoning cinnamon. Furthermore, the plant has been used as a traditional Chinese herbal medication. We explored the anticancer effect of Cuminaldehyde, an ingredient of the cortex of the plant, as well as the molecular biomarkers associated with carcinogenesis in human colorectal adenocarcinoma COLO 205 cells. The results show that Cuminaldehyde suppressed growth and induced apoptosis, as proved by depletion of the mitochondrial membrane potential, activation of both caspase-3 and -9, and morphological features of apoptosis. Moreover, Cuminaldehyde also led to lysosomal vacuolation with an upregulated volume of acidic compartment and cytotoxicity, together with inhibitions of both topoisomerase I and II activities. Additional study shows that the anticancer activity of Cuminaldehyde was observed in the model of nude mice. Our results suggest that the anticancer activity of Cuminaldehyde in vitro involved the suppression of cell proliferative markers, topoisomerase I as well as II, together with increase of pro-apoptotic molecules, associated with upregulated lysosomal vacuolation. On the other hand, in vivo, Cuminaldehyde diminished the tumor burden that would have a significant clinical impact. Furthermore, similar effects were observed in other tested cell lines. In short, our data suggest that Cuminaldehyde could be a drug for chemopreventive or anticancer therapy.
Yang Yang - One of the best experts on this subject based on the ideXlab platform.
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In vitro comparison of antioxidant capacity of cumin (Cuminum cyminum L.) oils extracted and purified by supercritical fluid extraction and molecular distillation and their main components
The FASEB Journal, 2012Co-Authors: Qinqin Chen, Zhilin Gan, Yang YangAbstract:The antioxidant activities (AOAs) of β-pinene, p-cymene, γ-terpinene, Cuminaldehyde, cumin oleoresin (COR, supercritical fluid extraction) and its molecular distillation products (CEO, DR&CT) were ...
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Preparative isolation and purification of Cuminaldehyde and p-menta-1,4-dien-7-al from the essential oil of Cuminum cyminum L. by high-speed counter-current chromatography
Analytica chimica acta, 2011Co-Authors: Qinqin Chen, Ping Liu, Yang YangAbstract:High-speed counter-current chromatography (HSCCC) technique in semi-preparative scale was successfully used in isolation and purification of Cuminaldehyde and p-menta-1,4-dien-7-al from the essential oil of Cuminum cyminum L. by using a two-phase solvent system composed of n-hexane-methanol-water (5:4:1, v/v/v). The targeted compounds were isolated, collected, purified by HSCCC in the head-tail mode, and then analyzed by gas chromatography (GC). A total of 12.72 ± 0.22 mg of Cuminaldehyde and 10.61 ± 0.27 mg of p-menta-1,4-dien-7-al were obtained from 50 mg of the essential oil of C. cyminum L. in less than 6 h, with purities of 95.42% and 97.21%, respectively. In addition to GC-EI/MS, the identity of the Cuminaldehyde was further confirmed with the retention time using the method of standard addition, while, the structural identification of p-menta-1,4-dien-7-al was performed with GC-EI/MS, (1)H NMR and (1)H-(1)H COSY.
Zhen Lijun - One of the best experts on this subject based on the ideXlab platform.
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novel Cuminaldehyde self emulsified nanoemulsion for enhanced antihepatotoxicity in carbon tetrachloride treated mice
Journal of Pharmacy and Pharmacology, 2019Co-Authors: Michael Adufrimpong, Wei Qiuyu, Caleb Kesse Firempong, Yusif Mohammed Mukhtar, Qiuxuan Yang, Emmanuel Omarisiaw, Zhen LijunAbstract:Objectives Cuminaldehyde self-emulsified nanoemulsion (CuA-SEN) was prepared and optimised to improve its oral bioavailability and antihepatotoxicity. Methods Cuminaldehyde self-emulsified nanoemulsion was developed through the self-nanoemulsification method using Box-Behnken Design (BBD) tool while appropriate physicochemical indices were evaluated. The optimised CuA-SEN was characterised via droplet size (DS), morphology, polydispersity index (PDI), zeta potential (ZP), entrapment efficiency, in-vitro release, and pharmacokinetic studies while its antihepatotoxicity was evaluated. Key findings Cuminaldehyde self-emulsified nanoemulsion with acceptable characteristics (mean DS-48.83 ± 1.06 nm; PDI-0.232 ± 0.140; ZP-29.92 ± 1.66 mV; EE-91.51 ± 0.44%; and drug-loading capacity (DL)-9.77 ± 0.75%) was formulated. In-vitro drug release of CuA-SEN significantly increased with an oral relative bioavailability of 171.02%. Oral administration of CuA-SEN to CCl4 -induced hepatotoxicity mice markedly increased the levels of superoxide dismutase, glutathione and catalase in serum. Also, CuA-SEN reduced the levels of tumour necrosis factor-alpha and interleukin-6 in both serum and liver tissues while aspartate aminotransferase, alanine aminotransferase and malonaldehyde levels were significantly decreased. Conclusions These findings showed that the improved bioavailability of Cuminaldehyde via SEN provided an effective approach for enhancing antioxidation, anti-inflammation and antihepatotoxicity of the drug.
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Novel Cuminaldehyde self-emulsified nanoemulsion for enhanced antihepatotoxicity in carbon tetrachloride-treated mice.
The Journal of pharmacy and pharmacology, 2019Co-Authors: Michael Adu-frimpong, Wei Qiuyu, Caleb Kesse Firempong, Yusif Mohammed Mukhtar, Qiuxuan Yang, Emmanuel Omari-siaw, Zhen LijunAbstract:Cuminaldehyde self-emulsified nanoemulsion (CuA-SEN) was prepared and optimised to improve its oral bioavailability and antihepatotoxicity. Cuminaldehyde self-emulsified nanoemulsion was developed through the self-nanoemulsification method using Box-Behnken Design (BBD) tool while appropriate physicochemical indices were evaluated. The optimised CuA-SEN was characterised via droplet size (DS), morphology, polydispersity index (PDI), zeta potential (ZP), entrapment efficiency, in-vitro release, and pharmacokinetic studies while its antihepatotoxicity was evaluated. Cuminaldehyde self-emulsified nanoemulsion with acceptable characteristics (mean DS-48.83 ± 1.06 nm; PDI-0.232 ± 0.140; ZP-29.92 ± 1.66 mV; EE-91.51 ± 0.44%; and drug-loading capacity (DL)-9.77 ± 0.75%) was formulated. In-vitro drug release of CuA-SEN significantly increased with an oral relative bioavailability of 171.02%. Oral administration of CuA-SEN to CCl4 -induced hepatotoxicity mice markedly increased the levels of superoxide dismutase, glutathione and catalase in serum. Also, CuA-SEN reduced the levels of tumour necrosis factor-alpha and interleukin-6 in both serum and liver tissues while aspartate aminotransferase, alanine aminotransferase and malonaldehyde levels were significantly decreased. These findings showed that the improved bioavailability of Cuminaldehyde via SEN provided an effective approach for enhancing antioxidation, anti-inflammation and antihepatotoxicity of the drug. © 2019 Royal Pharmaceutical Society.
Jaw-ming Cherng - One of the best experts on this subject based on the ideXlab platform.
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discovery of a novel anti cancer agent targeting both topoisomerase i ii as well as telomerase activities in human lung adenocarcinoma a549 cells in vitro and in vivo cinnamomum verum component Cuminaldehyde
Current Cancer Drug Targets, 2016Co-Authors: Tawei Chen, Shumei Yang, Hoyiu Wong, Kuoshen Chou, Jonathan Cherng, Kuendaw Tsai, Yi-heng Liu, Yang-tz Wang, Janise Cuizon, Jaw-ming CherngAbstract:Cinnamomum verum is used to make the spice cinnamon and has been used for more than 5000 years by both of the two most ancient forms of medicine in the words: Ayurveda and traditional Chinese herbal medicines for various applications such as adenopathy, rheumatism, dermatosis, dyspepsia, stroke, tumors, elephantiasis, trichomonas, yeast, and virus infections. We evaluated the anticancer effect of Cuminaldehyde (CuA), a constituent of the bark of the plant, and its underlying molecular biomarkers associated with carcinogenesis in human lung adenocarcinoma A549 cells. The results show that Cuminaldehyde suppressed proliferation and induced apoptosis as indicated by mitochondrial membrane potential loss, activation of caspase 3 and 9, increase in annexin V+PI+ cells, and morphological characteristics of apoptosis, including blebbing of plasma membrane, nuclear condensation, fragmentation, apoptotic body formation, and comet with elevated tail intensity and moment. In addition, Cuminaldehyde also induced lysosomal vacuolation with increased volume of acidic compartments (VAC), suppressions of both topoisomerase I & II as well as telomerase activities in a dose-dependent manner. Further study reveals the growth-inhibitory effect of Cuminaldehyde was also evident in a nude mice model. Taken together, the data suggest that the growth-inhibitory effect of Cuminaldehyde against A549 cells is accompanied by downregulations of proliferative control involving apoptosis, both topoisomerase I & II as well as telomerase activities, together with an upregulation of lysosomal vacuolation and VAC. Similar effects (including all of the above-mentioned effects) were found in other cell lines, including human lung squamous cell carcinoma NCI-H520 and colorectal adenocarcinoma COLO 205 (results not shown). Our data suggest that Cuminaldehyde could be a potential agent for anticancer therapy.
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Discovery of a Novel Anti-Cancer Agent Targeting Both Topoisomerase I & II as Well as Telomerase Activities in Human Lung Adenocarcinoma A549 Cells In Vitro and In Vivo: Cinnamomum verum Component Cuminaldehyde
Current cancer drug targets, 2016Co-Authors: Tawei Chen, Shumei Yang, Hoyiu Wong, Kuoshen Chou, Jonathan Cherng, Kuendaw Tsai, Yi-heng Liu, Yang-tz Wang, Janise Cuizon, Jaw-ming CherngAbstract:Cinnamomum verum is used to make the spice cinnamon and has been used for more than 5000 years by both of the two most ancient forms of medicine in the words: Ayurveda and traditional Chinese herbal medicines for various applications such as adenopathy, rheumatism, dermatosis, dyspepsia, stroke, tumors, elephantiasis, trichomonas, yeast, and virus infections. We evaluated the anticancer effect of Cuminaldehyde (CuA), a constituent of the bark of the plant, and its underlying molecular biomarkers associated with carcinogenesis in human lung adenocarcinoma A549 cells. The results show that Cuminaldehyde suppressed proliferation and induced apoptosis as indicated by mitochondrial membrane potential loss, activation of caspase 3 and 9, increase in annexin V+PI+ cells, and morphological characteristics of apoptosis, including blebbing of plasma membrane, nuclear condensation, fragmentation, apoptotic body formation, and comet with elevated tail intensity and moment. In addition, Cuminaldehyde also induced lysosomal vacuolation with increased volume of acidic compartments (VAC), suppressions of both topoisomerase I & II as well as telomerase activities in a dose-dependent manner. Further study reveals the growth-inhibitory effect of Cuminaldehyde was also evident in a nude mice model. Taken together, the data suggest that the growth-inhibitory effect of Cuminaldehyde against A549 cells is accompanied by downregulations of proliferative control involving apoptosis, both topoisomerase I & II as well as telomerase activities, together with an upregulation of lysosomal vacuolation and VAC. Similar effects (including all of the above-mentioned effects) were found in other cell lines, including human lung squamous cell carcinoma NCI-H520 and colorectal adenocarcinoma COLO 205 (results not shown). Our data suggest that Cuminaldehyde could be a potential agent for anticancer therapy.
Youngjoon Ahn - One of the best experts on this subject based on the ideXlab platform.
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toxicity of zanthoxylum piperitum and zanthoxylum armatum oil constituents and related compounds to stomoxys calcitrans diptera muscidae
Journal of Medical Entomology, 2012Co-Authors: Tran Trung Hieu, Soonil Kim, Youngjoon AhnAbstract:ABSTRACT Zanthoxylum plants (Rutaceae) have drawn attention because they contain insecticidal principles against insects. An assessment was made of the insecticidal and acetylcholinesterase (AChE) inhibitory activities of Zanthoxylum piperitum steam distillate and Zanthoxylum armatum seed oil, their 28 constituents, and eight structurally related compounds against female stable fly, Stomoxys calcitrans (L.). Results were compared with those of two organophosphorus insecticides chlorpyrifos and dichlorvos. Potent fumigant toxicity was observed with Cuminaldehyde, thymol, (1S)-(-)-verbenone, (-)-myrtenal, carvacrol, (S)-(Z)-verbenol, Zanthoxylum piperitum steam distillate, cuminyl alcohol, Zanthoxylum armatum seed oil, piperitone, (-)-(Z)-myrtanol, and citronellal (LC50, 0.075–0.456 µg/cm3). However, they were five orders of magnitude less toxic than either chlorpyrifos or dichlorvos. An in vitro bioassay using female fly heads indicates that strong AChE inhibition was produced by citronellyl acetate, α-pin...
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Enhanced repellency of binary mixtures of Zanthoxylum armatum seed oil, vanillin, and their aerosols to mosquitoes under laboratory and field conditions.
Journal of medical entomology, 2011Co-Authors: Hyung Wook Kwon, Soonil Kim, Kyu-sik Chang, J. Marshall Clark, Youngjoon AhnAbstract:The repellency of Zanthoxylum armatum seed oil (ZA-SO), alone or in combination with vanillin (VA), its six major constituents, and another four major previously known Zanthoxylum piperitum fruit oil constituents, as well as aerosol products containing 5 or 10% ZA-SO and 5% VA, was evaluated against female Aedes aegypti in laboratory and field studies. Results were then compared with those of N,N-diethyl-3-methylbenzamide (DEET) as a standard. Hand in cage laboratory tests showed that 0.2, 0.1, and 0.05 mg/cm2 ZA-SO resulted in > 92% protection through 30-min postexposure and was not significantly different than 0.05 mg/cm2 DEET. Skin treated with linalool and limonene (from Z. armatum) provided > 80% repellency to female Ae. aegypti at 10-min exposure, whereas Cuminaldehyde, citronellal, geranyl acetate, and cuminyl alcohol (from Zanthoxylum piperitum) provided > 90% protection during this same time period. Only Cuminaldehyde and citronellal provided complete protection comparable to DEET at 10-min postexposure. After that time, repellency of all plant constituents to mosquitoes was considerably decreased (< approximately 65%). An increase in repellency and duration of effectiveness was produced by a binary 1:4 mixture of ZA-SO and VA (0.05:0.2 mg/cm2) that was significantly more effective than 0.05 mg/cm2 DEET through 90 min. In field tests, an aerosol formulation containing 5 or 10% ZA-SO plus 5% VA gave 100% repellency at 60-min postexposure. Although these formulations were equal to the level of protection afforded by 10% DEET, repellency to the binary ZA-SO aerosol formulations at 90 min was significantly less effective than DEET. However, mixtures formulated from ZA-SO and VA merit further study as potential repellents for protection of humans and domestic animals from biting and nuisance caused by mosquitoes.
