The Experts below are selected from a list of 744 Experts worldwide ranked by ideXlab platform
Yi-ju Tsai - One of the best experts on this subject based on the ideXlab platform.
-
elevated galanin receptor type 2 primarily contributes to mechanical hypersensitivity after median nerve injury
PLOS ONE, 2018Co-Authors: Seu-hwa Chen, June-horng Lue, Yung-jung Hsiao, Shu-mei Lai, Hsin-ying Wang, Chi-te Lin, Ya-chin Chen, Yi-ju TsaiAbstract:In this study, we investigated temporal changes in galanin receptor type 2 (GalR2) expression in NF200-, galanin-, neuropeptide Y (NPY)-, and neuronal nitric oxide synthase (nNOS)-like immunoreactive (LI) dorsal root ganglion (DRG) neurons after median nerve chronic constriction injury (CCI), and the effects of GalR2 on c-Fos expression in the Cuneate Nucleus (CN). Double immunofluorescence labeling methods were used to appraise changes in GalR2 expression in NF200-LI, galanin-LI, NPY-LI, and nNOS-LI DRG neurons after CCI. The von Frey assay was used to assess the efficiency of intraplantar administration of saline, M871 (a GalR2 antagonist), or AR-M1896 (a GalR2 agonist) on neuropathic signs of rats with CCI. The effects of alterations in c-Fos expression were assessed in all treatments. The percentage of GalR2-LI neurons in lesioned DRGs increased and peaked at 1 week after CCI. We further detected that percentages of GalR2-LI neurons labeled for NF200, galanin, NPY, and nNOS significantly increased following CCI. Furthermore, M871 remarkably attenuated tactile allodynia, but the sensation was slightly aggravated by AR-M1896 after CCI. Consequentially, after electrical stimulation of the CCI-treated median nerve, the number of c-Fos-LI neurons in the Cuneate Nucleus (CN) was significantly reduced in the M871 group, whereas it increased in the AR-M1896 group. These results suggest that activation of GalR2, probably through NPY or nitric oxide, induces c-Fos expression in the CN and transmits mechanical allodynia sensations to the thalamus.
-
Elevated galanin receptor type 2 primarily contributes to mechanical hypersensitivity after median nerve injury - Fig 9
2018Co-Authors: Seu-hwa Chen, June-horng Lue, Yung-jung Hsiao, Shu-mei Lai, Hsin-ying Wang, Chi-te Lin, Ya-chin Chen, Yi-ju TsaiAbstract:Photographs showing c-Fos-like immunoreactive (LI) neurons in the middle region of the Cuneate Nucleus (CN) following unilateral electrical stimulation of the injured nerve at 1 week after median nerve chronic constriction injury (CCI) in the saline (A), AR-M1896 (B), and M871 (C) groups. Histograms show that the mean number of c-Fos-LI neurons in the M871 treatment group was significantly less than that in the saline treatment group (*** p
-
Pre-Emptive Treatment of Lidocaine Attenuates Neuropathic Pain and Reduces Pain-Related Biochemical Markers in the Rat Cuneate Nucleus in Median Nerve Chronic Constriction Injury Model
Hindawi Limited, 2012Co-Authors: Chi-te Lin, Seu-hwa Chen, Hsin-ying Wang, Yi-ju Tsai, Tzu-yu Lin, June-horng LueAbstract:This study investigates the effects of lidocaine pre-emptive treatment on neuropathic pain behavior, injury discharges of nerves, neuropeptide Y (NPY) and c-Fos expression in the Cuneate Nucleus (CN) after median nerve chronic constriction injury (CCI). Behavior tests demonstrated that the pre-emptive lidocaine treatment dose dependently delayed and attenuated the development of mechanical allodynia within a 28-day period. Electrophysiological recording was used to examine the changes in injury discharges of the nerves. An increase in frequency of injury discharges was observed and peaked at postelectrical stimulation stage in the presaline group, which was suppressed by lidocaine pre-emptive treatment in a dose-dependent manner. Lidocaine pretreatment also reduced the number of injury-induced NPY-like immunoreactive (NPY-LI) fibers and c-Fos-LI neurons within the CN in a dose-dependent manner. Furthermore, the mean number of c-Fos-LI neurons in the CN was significantly correlated to the NPY reduction level and the sign of mechanical allodynia following CCI
-
Neuropeptide Y Modulates C-Fos Protein Expression in the Cuneate Nucleus and Contributes to Mechanical Hypersensitivity Following Rat Median Nerve Injury
'Mary Ann Liebert Inc', 2011Co-Authors: Yi-ju TsaiAbstract:This study sought to investigate the effects of injury- induced neuropeptide Y (NPY) on c-Fos expression in the Cuneate neurons and neuropathic pain after median nerve injury. Four weeks after median nerve transection (MNT), the injured nerves stimulated at low intensity (0.1 mA) expressed significantly less NPY-like immunoreactive (NPY-LI ) fibers in the Cuneate Nucleus (CN) than those stimulated at high intensities (1.0mA and 10 mA). Conversely, a significantly higher number of c-Fos-LI cells were observed in the CN in rats stimulated with 0.1mA compared to those stimulated with 1.0mA or 10 mA. These results suggest that more NPY was released following low-intensity stimulation, and consequently fewer NPY- LI fibers and more c-Fos-LI cells were identified in the CN. Furthermore, the number of c-Fos -LI cells as well as the percentage of c-Fos-LI cu- neothalamic projection neurons (CTNs) in the CN was markedly decreased after injection of NPY receptor antagonist along with retrograde tract- tracing method, indicating that NPY regulated c-Fos expression. In rats with median nerve chronic constriction injury (CCI), intracerebroventricular injection of NPY aggravated mechanical allodynia and low- intensity stimulus-evoked c-Fos expression, both of which were reversed by injection of NPY receptor antagonist. However, thermal hyperalgesia was not affected by injection of these two reagents. Taken together, these findings suggest that more NPY release, following low- intensity electrical stimulation of the injured nerve, significantly induces c-Fos expression in the CTNs, which possibly provide the ascending thalamic transmission of neuropathic pain signals
-
Pre-Treatment with Lidocaine Suppresses Ectopic Discharges and Attenuates Neuropeptide Y and C-Fos Expressions in the Rat Cuneate Nucleus Following Median Nerve Transection
'Elsevier BV', 2011Co-Authors: Yi-ju TsaiAbstract:Following peripheral nerve injury, lidocaine application has been demonstrated to suppress injury discharges. However, there is very little information about the effects of lidocaine pre-treatment. The aim of the present study was to examine the effects of pre-treatment with lidocaine on injury discharges of the nerve, and neuropeptide Y (NPY) and c-Fos expression in the Cuneate Nucleus (CN) after median nerve transection (MNT ). Rats received either saline or 1%, 5%, or 10% lidocaine applied topically to the median nerve before nerve transection. Electrophysiological recording was used to examine the changes in injury discharges of the nerve at post-injection, transection, pre- and post- electrical stimulation stages in the different groups. Sequential immunohistochemistry was also used to identify the number of NPY-like immunoreactive (NPY-LI) fibers and c- Fos-LI cells in the corresponding CN . An increasing frequency of injury discharges was observed at all stages in the pre-saline group, which were suppressed by lidocaine pre-treatment in a dose-dependent manner. Lidocaine pre- treatment also attenuated the number of injury-induced NPY- LI fibers and c-Fos-LI neurons within the CN in a dose- dependent manner. Furthermore, expression of c-Fos-LI neurons in the CN was significantly reduced by an NPY receptor antagonist, indicating that NPY modulated c-Fos expression following MNT. These data suggest that preventing injury discharges with lidocaine pre-treatment can effectively attenuate central sensitization following MNT
June-horng Lue - One of the best experts on this subject based on the ideXlab platform.
-
elevated galanin receptor type 2 primarily contributes to mechanical hypersensitivity after median nerve injury
PLOS ONE, 2018Co-Authors: Seu-hwa Chen, June-horng Lue, Yung-jung Hsiao, Shu-mei Lai, Hsin-ying Wang, Chi-te Lin, Ya-chin Chen, Yi-ju TsaiAbstract:In this study, we investigated temporal changes in galanin receptor type 2 (GalR2) expression in NF200-, galanin-, neuropeptide Y (NPY)-, and neuronal nitric oxide synthase (nNOS)-like immunoreactive (LI) dorsal root ganglion (DRG) neurons after median nerve chronic constriction injury (CCI), and the effects of GalR2 on c-Fos expression in the Cuneate Nucleus (CN). Double immunofluorescence labeling methods were used to appraise changes in GalR2 expression in NF200-LI, galanin-LI, NPY-LI, and nNOS-LI DRG neurons after CCI. The von Frey assay was used to assess the efficiency of intraplantar administration of saline, M871 (a GalR2 antagonist), or AR-M1896 (a GalR2 agonist) on neuropathic signs of rats with CCI. The effects of alterations in c-Fos expression were assessed in all treatments. The percentage of GalR2-LI neurons in lesioned DRGs increased and peaked at 1 week after CCI. We further detected that percentages of GalR2-LI neurons labeled for NF200, galanin, NPY, and nNOS significantly increased following CCI. Furthermore, M871 remarkably attenuated tactile allodynia, but the sensation was slightly aggravated by AR-M1896 after CCI. Consequentially, after electrical stimulation of the CCI-treated median nerve, the number of c-Fos-LI neurons in the Cuneate Nucleus (CN) was significantly reduced in the M871 group, whereas it increased in the AR-M1896 group. These results suggest that activation of GalR2, probably through NPY or nitric oxide, induces c-Fos expression in the CN and transmits mechanical allodynia sensations to the thalamus.
-
Elevated galanin receptor type 2 primarily contributes to mechanical hypersensitivity after median nerve injury - Fig 9
2018Co-Authors: Seu-hwa Chen, June-horng Lue, Yung-jung Hsiao, Shu-mei Lai, Hsin-ying Wang, Chi-te Lin, Ya-chin Chen, Yi-ju TsaiAbstract:Photographs showing c-Fos-like immunoreactive (LI) neurons in the middle region of the Cuneate Nucleus (CN) following unilateral electrical stimulation of the injured nerve at 1 week after median nerve chronic constriction injury (CCI) in the saline (A), AR-M1896 (B), and M871 (C) groups. Histograms show that the mean number of c-Fos-LI neurons in the M871 treatment group was significantly less than that in the saline treatment group (*** p
-
Pre-Emptive Treatment of Lidocaine Attenuates Neuropathic Pain and Reduces Pain-Related Biochemical Markers in the Rat Cuneate Nucleus in Median Nerve Chronic Constriction Injury Model
Hindawi Limited, 2012Co-Authors: Chi-te Lin, Seu-hwa Chen, Hsin-ying Wang, Yi-ju Tsai, Tzu-yu Lin, June-horng LueAbstract:This study investigates the effects of lidocaine pre-emptive treatment on neuropathic pain behavior, injury discharges of nerves, neuropeptide Y (NPY) and c-Fos expression in the Cuneate Nucleus (CN) after median nerve chronic constriction injury (CCI). Behavior tests demonstrated that the pre-emptive lidocaine treatment dose dependently delayed and attenuated the development of mechanical allodynia within a 28-day period. Electrophysiological recording was used to examine the changes in injury discharges of the nerves. An increase in frequency of injury discharges was observed and peaked at postelectrical stimulation stage in the presaline group, which was suppressed by lidocaine pre-emptive treatment in a dose-dependent manner. Lidocaine pretreatment also reduced the number of injury-induced NPY-like immunoreactive (NPY-LI) fibers and c-Fos-LI neurons within the CN in a dose-dependent manner. Furthermore, the mean number of c-Fos-LI neurons in the CN was significantly correlated to the NPY reduction level and the sign of mechanical allodynia following CCI
-
synaptic relationships between induced neuropeptide y like immunoreactive terminals and cuneothalamic projection neurons in the rat Cuneate Nucleus following median nerve transection
Journal of Chemical Neuroanatomy, 2008Co-Authors: Jiannhorng Yeh, June-horng Lue, Hsin-ying Wang, Chunta Huang, Yi-ju TsaiAbstract:Previous studies have demonstrated that following complete median nerve transection (CMNT), neuropeptide Y-like immunoreactive (NPY-LI) fibers were dramatically increased and predominantly expressed in the ventral portion of the middle Cuneate Nucleus (CN), reaching maximum numbers at four weeks. Ultrastructurally, NPY-LI terminals made axodendritic synapses, but the postsynaptic elements are unknown. In the present study, using retrograde tract-tracing of wheat germ agglutinin conjugated with horseradish peroxidase (WGA-HRP) and NPY immunocytochemistry we examined the synaptic relationships between cuneothalamic projection neurons (CTNs) and NPY-LI terminals in the rat CN following CMNT. The injury-induced NPY-LI fibers were distributed throughout the rostrocaudal extent of the CN. Further, the greatest number of HRP-labeled CTNs was observed in the ventral portion of the middle CN. Ultrastructurally, the NPY-LI terminals made asymmetric axodendritic synaptic contact with the HRP-labeling CTN dendrites. These data suggest that injury-induced NPY may modulate the excitability of CTNs, and thus, play a role in the transmission of neuropathic sensation following median nerve injury.
-
aβ fiber intensity stimulation of chronically constricted median nerve induces c fos expression in thalamic projection neurons of the Cuneate Nucleus in rats with behavioral signs of neuropathic pain
Brain Research, 2001Co-Authors: Anshiou Day, June-horng Lue, Weizen Sun, Jengyung Shieh, Chenyuan WenAbstract:This study was aimed to investigate the possible involvement of neurons in the Cuneate Nucleus (CN) in the processing of Aβ afferent inputs evoked by electrical stimulation of constricted median nerve in rats with behavioral signs of neuropathic pain. Immunohistochemical localization of Fos protein was used to examine the neuronal activation, and the combination of Fos immunohistochemistry with the retrograde labeling of Fluoro-Gold (FG) injected into the ventrobasal complex of the thalamus was used to characterize the activated neurons. Two weeks after unilateral median nerve constriction injury, the rats exhibited behavioral signs of neuropathic pain in the affected forepaws. In rats after nerve injury but without electrical stimulation, some Fos-like immunoreactive (Fos-LI) neurons were detected in the dorsal horn of the seventh cervical segment (C7) but none was found in the CN. Similar features were also noted when the stimulation of the intact median nerve served as an additional control. After Aβ-fiber intensity stimulation of the previously constricted median nerve, an increase in number of Fos-LI neurons occurred in the medial half of the ipsilateral C7 dorsal horn as well as in the ipsilateral CN. In the latter, the Fos-LI neurons were located in the median nerve projection territory throughout the Nucleus. Most of the Fos-LI neurons were distributed in the middle region of the CN, with about 78% of them emitting FG fluorescence indicating that they were cuneothalamic projection neurons. The results of this study suggest that the dorsal column-medial lemniscal system may contribute to the transmission and modulation of Aβ-fiber mediated neuropathic pain signals.
Luis Villanueva - One of the best experts on this subject based on the ideXlab platform.
-
organization of diencephalic projections from the medullary subNucleus reticularis dorsalis and the adjacent Cuneate Nucleus a retrograde and anterograde tracer study in the rat
The Journal of Comparative Neurology, 1998Co-Authors: Luis Villanueva, Daniel Le Bars, C Desbois, Jeanfrancois BernardAbstract:The distribution and organization of diencephalic projections from the subNucleus reticularis dorsalis (SRD) and the neighbouring Cuneate Nucleus (Cu) were studied in the rat by using microinjections of Phaseolus vulgaris leucoagglutinin in SRD and Cu and wheat germ agglutinin-apo horseradish peroxidase-gold in some selected thalamic areas. As previously reported, the efferent projections from the Cu were essentially contralateral and terminated mainly in the ventroposterolateral thalamic Nucleus. Less dense terminals from the Cu were also observed in the posterior thalamic group, the ventral aspect of the zona incerta and the caudal and dorsal portion of the reuniens area. Retrograde tracer injections in the medial ventroposterolateral thalamic Nucleus labeled numerous cells in the contralateral Cu, with a smaller number in the gracile Nucleus. From the SRD, terminals were observed in the lateral aspect of the ventromedial thalamic Nucleus, the lateral parafascicular area and, to a lesser extent, in the ventral aspect of the zona incerta and the core of the reuniens area. Retrograde tracer injections in the lateral part of the ventromedial thalamic Nucleus labeled cells in the caudal medulla, many of which were located in the dorsal-most aspect of the SRD throughout its caudo-rostral extent. The existence of SRD-thalamic connections reinforces the idea that the caudal reticular formation is an important nociceptive relay to the thalamus. Our data shed new light on old hypotheses suggesting that, in addition to spino-thalamic pathways, spino-reticulo-thalamic pathways may play an important role in distributing pain signals to the forebrain.
-
organization of efferent projections from the spinal cervical enlargement to the medullary subNucleus reticularis dorsalis and the adjacent Cuneate Nucleus a pha l study in the rat
The Journal of Comparative Neurology, 1996Co-Authors: Patrick Raboisson, Daniel Le Bars, Radhouane Dallel, Jeanfrancois Bernard, Luis VillanuevaAbstract:The distribution and organization of projections from the spinal cervical enlargement to subNucleus rcticularis dorsalis (SRD) and the neighbouring Cuneate Nucleus (Cu) area was studied in the rat by using microinjections of Phaseolus uulgaris leucoagglutinin (PHA-L) into different laminae around the C7 level. The Cu received very dense projections from the dorsal horn, with the highest density being observed following injections into the medial part of laminae 111-IV. The SRD received dense projections from laminae V-VII of the cervical enlargement, particularly from the reticular and medial aspects of laminaV, lamina VI, and the dorsal part of lamina VII. By contrast, the superficial part of the dorsal horn (laminae I to IV) and the dorsal part of lamina X provided only sparse projections to the SRD. Clusters of labelled terminals and boutons were observed mainly in the SRD areas subjacent to the Cu. In the caudorostral axis, labelled terminals were spread along the whole SRD from the cervicomedullary junction up to the caudal-most part of the area postrema. Contralateral projections to the SRD were scarce and were observed mainly after injections into the medial part of laminae VI-VII. These data give further support to the proposal that there are two parallel systems in neighbouring structures of the caudal medulla, viz. the Cu and the SRD, which, respectively, relay lemniscal and nociceptive information from the spinal cord to the thalamus.
-
distribution of spinal cord projections from the medullary subNucleus reticularis dorsalis and the adjacent Cuneate Nucleus a phaseolus vulgaris leucoagglutinin study in the rat
The Journal of Comparative Neurology, 1995Co-Authors: Luis Villanueva, Jean Franicois Bernard, Daniel Le BarsAbstract:The distribution and organization of descending spinal projections from the dorsal part of the caudal medulla were studied in the rat following injections of Phaseolus vulgaris leucoagglutinin into small areas of the subNucleus reticularis dorsalis (SRD) and the adjacent Cuneate Nucleus (Cu). The caudal aspect of the Cu projected only to the dorsal horn of the ipsilateral cervical cord via the dorsal funiculus. These projections were mainly to laminae I, IV, and V. More ventrally located reticular structures projected to the full length of the cord. Fibers originating from the SRD travelled through the ipsilateral dorsolateral funiculus and terminated within the deep dorsal horn and upper layers of the ventral horn, mainly in laminae V–VII. Fibers originating from subNucleus reticularis ventralis (SRV) travelled ipsilaterally through the lateral and ventrolateral funiculi and bilaterally through the ventromedial funiculus. These fibers terminated within the ventral horn. The density of labeling within the gray matter varied at different levels of the cord was as follows: cervical > sacral > thoracic > lumbar. The reciprocal connections between the caudal medulla and the spinal cord suggest that the former is an important link in feedback loops that regulate spinal outflow. © 1995 Wiley-Liss, Inc.
Daniel Le Bars - One of the best experts on this subject based on the ideXlab platform.
-
organization of diencephalic projections from the medullary subNucleus reticularis dorsalis and the adjacent Cuneate Nucleus a retrograde and anterograde tracer study in the rat
The Journal of Comparative Neurology, 1998Co-Authors: Luis Villanueva, Daniel Le Bars, C Desbois, Jeanfrancois BernardAbstract:The distribution and organization of diencephalic projections from the subNucleus reticularis dorsalis (SRD) and the neighbouring Cuneate Nucleus (Cu) were studied in the rat by using microinjections of Phaseolus vulgaris leucoagglutinin in SRD and Cu and wheat germ agglutinin-apo horseradish peroxidase-gold in some selected thalamic areas. As previously reported, the efferent projections from the Cu were essentially contralateral and terminated mainly in the ventroposterolateral thalamic Nucleus. Less dense terminals from the Cu were also observed in the posterior thalamic group, the ventral aspect of the zona incerta and the caudal and dorsal portion of the reuniens area. Retrograde tracer injections in the medial ventroposterolateral thalamic Nucleus labeled numerous cells in the contralateral Cu, with a smaller number in the gracile Nucleus. From the SRD, terminals were observed in the lateral aspect of the ventromedial thalamic Nucleus, the lateral parafascicular area and, to a lesser extent, in the ventral aspect of the zona incerta and the core of the reuniens area. Retrograde tracer injections in the lateral part of the ventromedial thalamic Nucleus labeled cells in the caudal medulla, many of which were located in the dorsal-most aspect of the SRD throughout its caudo-rostral extent. The existence of SRD-thalamic connections reinforces the idea that the caudal reticular formation is an important nociceptive relay to the thalamus. Our data shed new light on old hypotheses suggesting that, in addition to spino-thalamic pathways, spino-reticulo-thalamic pathways may play an important role in distributing pain signals to the forebrain.
-
organization of efferent projections from the spinal cervical enlargement to the medullary subNucleus reticularis dorsalis and the adjacent Cuneate Nucleus a pha l study in the rat
The Journal of Comparative Neurology, 1996Co-Authors: Patrick Raboisson, Daniel Le Bars, Radhouane Dallel, Jeanfrancois Bernard, Luis VillanuevaAbstract:The distribution and organization of projections from the spinal cervical enlargement to subNucleus rcticularis dorsalis (SRD) and the neighbouring Cuneate Nucleus (Cu) area was studied in the rat by using microinjections of Phaseolus uulgaris leucoagglutinin (PHA-L) into different laminae around the C7 level. The Cu received very dense projections from the dorsal horn, with the highest density being observed following injections into the medial part of laminae 111-IV. The SRD received dense projections from laminae V-VII of the cervical enlargement, particularly from the reticular and medial aspects of laminaV, lamina VI, and the dorsal part of lamina VII. By contrast, the superficial part of the dorsal horn (laminae I to IV) and the dorsal part of lamina X provided only sparse projections to the SRD. Clusters of labelled terminals and boutons were observed mainly in the SRD areas subjacent to the Cu. In the caudorostral axis, labelled terminals were spread along the whole SRD from the cervicomedullary junction up to the caudal-most part of the area postrema. Contralateral projections to the SRD were scarce and were observed mainly after injections into the medial part of laminae VI-VII. These data give further support to the proposal that there are two parallel systems in neighbouring structures of the caudal medulla, viz. the Cu and the SRD, which, respectively, relay lemniscal and nociceptive information from the spinal cord to the thalamus.
-
distribution of spinal cord projections from the medullary subNucleus reticularis dorsalis and the adjacent Cuneate Nucleus a phaseolus vulgaris leucoagglutinin study in the rat
The Journal of Comparative Neurology, 1995Co-Authors: Luis Villanueva, Jean Franicois Bernard, Daniel Le BarsAbstract:The distribution and organization of descending spinal projections from the dorsal part of the caudal medulla were studied in the rat following injections of Phaseolus vulgaris leucoagglutinin into small areas of the subNucleus reticularis dorsalis (SRD) and the adjacent Cuneate Nucleus (Cu). The caudal aspect of the Cu projected only to the dorsal horn of the ipsilateral cervical cord via the dorsal funiculus. These projections were mainly to laminae I, IV, and V. More ventrally located reticular structures projected to the full length of the cord. Fibers originating from the SRD travelled through the ipsilateral dorsolateral funiculus and terminated within the deep dorsal horn and upper layers of the ventral horn, mainly in laminae V–VII. Fibers originating from subNucleus reticularis ventralis (SRV) travelled ipsilaterally through the lateral and ventrolateral funiculi and bilaterally through the ventromedial funiculus. These fibers terminated within the ventral horn. The density of labeling within the gray matter varied at different levels of the cord was as follows: cervical > sacral > thoracic > lumbar. The reciprocal connections between the caudal medulla and the spinal cord suggest that the former is an important link in feedback loops that regulate spinal outflow. © 1995 Wiley-Liss, Inc.
Seu-hwa Chen - One of the best experts on this subject based on the ideXlab platform.
-
elevated galanin receptor type 2 primarily contributes to mechanical hypersensitivity after median nerve injury
PLOS ONE, 2018Co-Authors: Seu-hwa Chen, June-horng Lue, Yung-jung Hsiao, Shu-mei Lai, Hsin-ying Wang, Chi-te Lin, Ya-chin Chen, Yi-ju TsaiAbstract:In this study, we investigated temporal changes in galanin receptor type 2 (GalR2) expression in NF200-, galanin-, neuropeptide Y (NPY)-, and neuronal nitric oxide synthase (nNOS)-like immunoreactive (LI) dorsal root ganglion (DRG) neurons after median nerve chronic constriction injury (CCI), and the effects of GalR2 on c-Fos expression in the Cuneate Nucleus (CN). Double immunofluorescence labeling methods were used to appraise changes in GalR2 expression in NF200-LI, galanin-LI, NPY-LI, and nNOS-LI DRG neurons after CCI. The von Frey assay was used to assess the efficiency of intraplantar administration of saline, M871 (a GalR2 antagonist), or AR-M1896 (a GalR2 agonist) on neuropathic signs of rats with CCI. The effects of alterations in c-Fos expression were assessed in all treatments. The percentage of GalR2-LI neurons in lesioned DRGs increased and peaked at 1 week after CCI. We further detected that percentages of GalR2-LI neurons labeled for NF200, galanin, NPY, and nNOS significantly increased following CCI. Furthermore, M871 remarkably attenuated tactile allodynia, but the sensation was slightly aggravated by AR-M1896 after CCI. Consequentially, after electrical stimulation of the CCI-treated median nerve, the number of c-Fos-LI neurons in the Cuneate Nucleus (CN) was significantly reduced in the M871 group, whereas it increased in the AR-M1896 group. These results suggest that activation of GalR2, probably through NPY or nitric oxide, induces c-Fos expression in the CN and transmits mechanical allodynia sensations to the thalamus.
-
Elevated galanin receptor type 2 primarily contributes to mechanical hypersensitivity after median nerve injury - Fig 9
2018Co-Authors: Seu-hwa Chen, June-horng Lue, Yung-jung Hsiao, Shu-mei Lai, Hsin-ying Wang, Chi-te Lin, Ya-chin Chen, Yi-ju TsaiAbstract:Photographs showing c-Fos-like immunoreactive (LI) neurons in the middle region of the Cuneate Nucleus (CN) following unilateral electrical stimulation of the injured nerve at 1 week after median nerve chronic constriction injury (CCI) in the saline (A), AR-M1896 (B), and M871 (C) groups. Histograms show that the mean number of c-Fos-LI neurons in the M871 treatment group was significantly less than that in the saline treatment group (*** p
-
Pre-Emptive Treatment of Lidocaine Attenuates Neuropathic Pain and Reduces Pain-Related Biochemical Markers in the Rat Cuneate Nucleus in Median Nerve Chronic Constriction Injury Model
Hindawi Limited, 2012Co-Authors: Chi-te Lin, Seu-hwa Chen, Hsin-ying Wang, Yi-ju Tsai, Tzu-yu Lin, June-horng LueAbstract:This study investigates the effects of lidocaine pre-emptive treatment on neuropathic pain behavior, injury discharges of nerves, neuropeptide Y (NPY) and c-Fos expression in the Cuneate Nucleus (CN) after median nerve chronic constriction injury (CCI). Behavior tests demonstrated that the pre-emptive lidocaine treatment dose dependently delayed and attenuated the development of mechanical allodynia within a 28-day period. Electrophysiological recording was used to examine the changes in injury discharges of the nerves. An increase in frequency of injury discharges was observed and peaked at postelectrical stimulation stage in the presaline group, which was suppressed by lidocaine pre-emptive treatment in a dose-dependent manner. Lidocaine pretreatment also reduced the number of injury-induced NPY-like immunoreactive (NPY-LI) fibers and c-Fos-LI neurons within the CN in a dose-dependent manner. Furthermore, the mean number of c-Fos-LI neurons in the CN was significantly correlated to the NPY reduction level and the sign of mechanical allodynia following CCI
