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Alfredo Tursib - One of the best experts on this subject based on the ideXlab platform.
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allergens of arizona cypress Cupressus arizonica pollen characterization of the pollen extract and identification of the allergenic components
The Journal of Allergy and Clinical Immunology, 1994Co-Authors: Gabriella Di Felicea, Maria Filomena Caiaffab, Giuseppe Barilettob, Claudia Affernia, Roberto Di Paolab, Adriano Maria, Sabrina Palumboa, Raffaella Tinghinoa, Federica Sallustoa, Alfredo TursibAbstract:Abstract Species of the Cupressaceae family are an important cause of respiratory allergies in countries with a Mediterranean climate. An allergenic extract from Cupressus arizonica pollen was prepared with two extraction steps followed by ammonium sulfate precipitation, giving a protein yield of about 3%. Cupressus arizonica pollen extract was also characterized by means of sodium dodecylsulfate–polyacrylamide gel electrophoresis, followed by IgE and IgG immunoblotting and lectin blotting. IgE reactivity was restricted to six components, whereas IgG binding showed a more complex pattern. A 43 kd component, predominant both in its intensity and frequency of recognition by human IgE antibodies, was identified as the major allergen of C. arizonica. Four of the six IgE-binding components, including the major allergen, seem to be glycoproteins, as confirmed by the lectin blotting analysis. The extract produced inhouse was used to set up an immunoenzymatic test to evaluate the specific IgE binding in a panel of sera from 33 immunotherapy-free subjects who were monosensitized to cypress pollen. The percent of positivity obtained was much higher than that reported in the literature for commercial immunoassays. (J ALLERGY CLIN IMMUNOL 1994;94:547-55.)
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allergens of arizona cypress Cupressus arizonica pollen characterization of the pollen extract and identification of the allergenic components
The Journal of Allergy and Clinical Immunology, 1994Co-Authors: Gabriella Di Felicea, Maria Filomena Caiaffab, Giuseppe Barilettob, Claudia Affernia, Roberto Di Paolab, Adriano Maria, Sabrina Palumboa, Raffaella Tinghinoa, Federica Sallustoa, Alfredo TursibAbstract:Species of the Cupressaceae family are an important cause of respiratory allergies in countries with a Mediterranean climate. An allergenic extract from Cupressus arizonica pollen was prepared with two extraction steps followed by ammonium sulfate precipitation, giving a protein yield of about 3%. Cupressus arizonica pollen extract was also characterized by means of sodium dodecylsulfate-polyacrylamide gel electrophoresis, followed by IgE and IgG immunoblotting and lectin blotting. IgE reactivity was restricted to six components, whereas IgG binding showed a more complex pattern. A 43 kd component, predominant both in its intensity and frequency of recognition by human IgE antibodies, was identified as the major allergen of C. arizonica. Four of the six IgE-binding components, including the major allergen, seem to be glycoproteins, as confirmed by the lectin blotting analysis. The extract produced inhouse was used to set up an immunoenzymatic test to evaluate the specific IgE binding in a panel of sera from 33 immunotherapy-free subjects who were monosensitized to cypress pollen. The percent of positivity obtained was much higher than that reported in the literature for commercial immunoassays.
Pastor Ferrer Elsa - One of the best experts on this subject based on the ideXlab platform.
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Technical Note TN 2.2 Fuel load sampling of a Cupressus sempervirens hedge
2019Co-Authors: Agueda Costafreda Alba, Muñoz Navarro, Juan Antonio, Pastor Ferrer ElsaAbstract:In this document, data from a destructive sampling of a Cupressus sempervirens hedgerow are shown, as well as the followed methodology. Measures of sizes and weights have been taken. With these data, moisture content, fuel load and bulk density have been calculated. Different fuel classes (state and diameter) have been taken into account
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Technical Note TN 2.2 Fuel load sampling of a Cupressus sempervirens hedge
2019Co-Authors: Agueda Costafreda Alba, Muñoz Navarro, Juan Antonio, Pastor Ferrer ElsaAbstract:In this document, data from a destructive sampling of a Cupressus sempervirens hedgerow are shown, as well as the followed methodology. Measures of sizes and weights have been taken. With these data, moisture content, fuel load and bulk density have been calculated. Different fuel classes (state and diameter) have been taken into account.Postprint (updated version
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Technical Note TN 2.4. Fuel sampling, tree ignition and burning tests in ADAI facilities
2019Co-Authors: Muñoz Navarro, Juan Antonio, Pastor Ferrer Elsa, Planas Cuchi Eulàlia, Agueda Costafreda AlbaAbstract:In this document, the followed methodology to characterize several natural fuels (trees of Cupressus arizonica, Cupressocyparis leylandii, Prunus laurocerasus and Thuja occidentalis) is presented. Also, the ignition method and the equipment used to monitor the burning of these fuels in two different configurations are described. These activities were performed in ADAI facilities (Lousa, Portugal).Preprin
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Technical Note TN 2.4. Fuel sampling, tree ignition and burning tests in ADAI facilities
2019Co-Authors: Muñoz Navarro, Juan Antonio, Pastor Ferrer Elsa, Planas Cuchi Eulàlia, Agueda Costafreda AlbaAbstract:In this document, the followed methodology to characterize several natural fuels (trees of Cupressus arizonica, Cupressocyparis leylandii, Prunus laurocerasus and Thuja occidentalis) is presented. Also, the ignition method and the equipment used to monitor the burning of these fuels in two different configurations are described. These activities were performed in ADAI facilities (Lousa, Portugal)
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Fuel load sampling of a Cupressus sempervirens hedge in Parc de Cervantes, Barcelona
2019Co-Authors: Agueda Costafreda Alba, Pastor Ferrer ElsaAbstract:In this document, data from three destructive samplings performed in a Cupressus sempervirens hedge in Parc de Cervantes (Barcelona) are shown; the methodology applied is also described. Measurements of fuel load have been taken, and moisture content, mass distribution and bulk density have been calculated. Different fuel classes (according to status and diameter) have been taken into account
Agueda Costafreda Alba - One of the best experts on this subject based on the ideXlab platform.
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Technical Note TN 2.2 Fuel load sampling of a Cupressus sempervirens hedge
2019Co-Authors: Agueda Costafreda Alba, Muñoz Navarro, Juan Antonio, Pastor Ferrer ElsaAbstract:In this document, data from a destructive sampling of a Cupressus sempervirens hedgerow are shown, as well as the followed methodology. Measures of sizes and weights have been taken. With these data, moisture content, fuel load and bulk density have been calculated. Different fuel classes (state and diameter) have been taken into account
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Technical Note TN 2.2 Fuel load sampling of a Cupressus sempervirens hedge
2019Co-Authors: Agueda Costafreda Alba, Muñoz Navarro, Juan Antonio, Pastor Ferrer ElsaAbstract:In this document, data from a destructive sampling of a Cupressus sempervirens hedgerow are shown, as well as the followed methodology. Measures of sizes and weights have been taken. With these data, moisture content, fuel load and bulk density have been calculated. Different fuel classes (state and diameter) have been taken into account.Postprint (updated version
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Technical Note TN 2.4. Fuel sampling, tree ignition and burning tests in ADAI facilities
2019Co-Authors: Muñoz Navarro, Juan Antonio, Pastor Ferrer Elsa, Planas Cuchi Eulàlia, Agueda Costafreda AlbaAbstract:In this document, the followed methodology to characterize several natural fuels (trees of Cupressus arizonica, Cupressocyparis leylandii, Prunus laurocerasus and Thuja occidentalis) is presented. Also, the ignition method and the equipment used to monitor the burning of these fuels in two different configurations are described. These activities were performed in ADAI facilities (Lousa, Portugal).Preprin
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Technical Note TN 2.4. Fuel sampling, tree ignition and burning tests in ADAI facilities
2019Co-Authors: Muñoz Navarro, Juan Antonio, Pastor Ferrer Elsa, Planas Cuchi Eulàlia, Agueda Costafreda AlbaAbstract:In this document, the followed methodology to characterize several natural fuels (trees of Cupressus arizonica, Cupressocyparis leylandii, Prunus laurocerasus and Thuja occidentalis) is presented. Also, the ignition method and the equipment used to monitor the burning of these fuels in two different configurations are described. These activities were performed in ADAI facilities (Lousa, Portugal)
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Fuel load sampling of a Cupressus sempervirens hedge in Parc de Cervantes, Barcelona
2019Co-Authors: Agueda Costafreda Alba, Pastor Ferrer ElsaAbstract:In this document, data from three destructive samplings performed in a Cupressus sempervirens hedge in Parc de Cervantes (Barcelona) are shown; the methodology applied is also described. Measurements of fuel load have been taken, and moisture content, mass distribution and bulk density have been calculated. Different fuel classes (according to status and diameter) have been taken into account
Gabriella Di Felicea - One of the best experts on this subject based on the ideXlab platform.
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allergens of arizona cypress Cupressus arizonica pollen characterization of the pollen extract and identification of the allergenic components
The Journal of Allergy and Clinical Immunology, 1994Co-Authors: Gabriella Di Felicea, Maria Filomena Caiaffab, Giuseppe Barilettob, Claudia Affernia, Roberto Di Paolab, Adriano Maria, Sabrina Palumboa, Raffaella Tinghinoa, Federica Sallustoa, Alfredo TursibAbstract:Abstract Species of the Cupressaceae family are an important cause of respiratory allergies in countries with a Mediterranean climate. An allergenic extract from Cupressus arizonica pollen was prepared with two extraction steps followed by ammonium sulfate precipitation, giving a protein yield of about 3%. Cupressus arizonica pollen extract was also characterized by means of sodium dodecylsulfate–polyacrylamide gel electrophoresis, followed by IgE and IgG immunoblotting and lectin blotting. IgE reactivity was restricted to six components, whereas IgG binding showed a more complex pattern. A 43 kd component, predominant both in its intensity and frequency of recognition by human IgE antibodies, was identified as the major allergen of C. arizonica. Four of the six IgE-binding components, including the major allergen, seem to be glycoproteins, as confirmed by the lectin blotting analysis. The extract produced inhouse was used to set up an immunoenzymatic test to evaluate the specific IgE binding in a panel of sera from 33 immunotherapy-free subjects who were monosensitized to cypress pollen. The percent of positivity obtained was much higher than that reported in the literature for commercial immunoassays. (J ALLERGY CLIN IMMUNOL 1994;94:547-55.)
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allergens of arizona cypress Cupressus arizonica pollen characterization of the pollen extract and identification of the allergenic components
The Journal of Allergy and Clinical Immunology, 1994Co-Authors: Gabriella Di Felicea, Maria Filomena Caiaffab, Giuseppe Barilettob, Claudia Affernia, Roberto Di Paolab, Adriano Maria, Sabrina Palumboa, Raffaella Tinghinoa, Federica Sallustoa, Alfredo TursibAbstract:Species of the Cupressaceae family are an important cause of respiratory allergies in countries with a Mediterranean climate. An allergenic extract from Cupressus arizonica pollen was prepared with two extraction steps followed by ammonium sulfate precipitation, giving a protein yield of about 3%. Cupressus arizonica pollen extract was also characterized by means of sodium dodecylsulfate-polyacrylamide gel electrophoresis, followed by IgE and IgG immunoblotting and lectin blotting. IgE reactivity was restricted to six components, whereas IgG binding showed a more complex pattern. A 43 kd component, predominant both in its intensity and frequency of recognition by human IgE antibodies, was identified as the major allergen of C. arizonica. Four of the six IgE-binding components, including the major allergen, seem to be glycoproteins, as confirmed by the lectin blotting analysis. The extract produced inhouse was used to set up an immunoenzymatic test to evaluate the specific IgE binding in a panel of sera from 33 immunotherapy-free subjects who were monosensitized to cypress pollen. The percent of positivity obtained was much higher than that reported in the literature for commercial immunoassays.
Sabrina Palumboa - One of the best experts on this subject based on the ideXlab platform.
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allergens of arizona cypress Cupressus arizonica pollen characterization of the pollen extract and identification of the allergenic components
The Journal of Allergy and Clinical Immunology, 1994Co-Authors: Gabriella Di Felicea, Maria Filomena Caiaffab, Giuseppe Barilettob, Claudia Affernia, Roberto Di Paolab, Adriano Maria, Sabrina Palumboa, Raffaella Tinghinoa, Federica Sallustoa, Alfredo TursibAbstract:Abstract Species of the Cupressaceae family are an important cause of respiratory allergies in countries with a Mediterranean climate. An allergenic extract from Cupressus arizonica pollen was prepared with two extraction steps followed by ammonium sulfate precipitation, giving a protein yield of about 3%. Cupressus arizonica pollen extract was also characterized by means of sodium dodecylsulfate–polyacrylamide gel electrophoresis, followed by IgE and IgG immunoblotting and lectin blotting. IgE reactivity was restricted to six components, whereas IgG binding showed a more complex pattern. A 43 kd component, predominant both in its intensity and frequency of recognition by human IgE antibodies, was identified as the major allergen of C. arizonica. Four of the six IgE-binding components, including the major allergen, seem to be glycoproteins, as confirmed by the lectin blotting analysis. The extract produced inhouse was used to set up an immunoenzymatic test to evaluate the specific IgE binding in a panel of sera from 33 immunotherapy-free subjects who were monosensitized to cypress pollen. The percent of positivity obtained was much higher than that reported in the literature for commercial immunoassays. (J ALLERGY CLIN IMMUNOL 1994;94:547-55.)
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allergens of arizona cypress Cupressus arizonica pollen characterization of the pollen extract and identification of the allergenic components
The Journal of Allergy and Clinical Immunology, 1994Co-Authors: Gabriella Di Felicea, Maria Filomena Caiaffab, Giuseppe Barilettob, Claudia Affernia, Roberto Di Paolab, Adriano Maria, Sabrina Palumboa, Raffaella Tinghinoa, Federica Sallustoa, Alfredo TursibAbstract:Species of the Cupressaceae family are an important cause of respiratory allergies in countries with a Mediterranean climate. An allergenic extract from Cupressus arizonica pollen was prepared with two extraction steps followed by ammonium sulfate precipitation, giving a protein yield of about 3%. Cupressus arizonica pollen extract was also characterized by means of sodium dodecylsulfate-polyacrylamide gel electrophoresis, followed by IgE and IgG immunoblotting and lectin blotting. IgE reactivity was restricted to six components, whereas IgG binding showed a more complex pattern. A 43 kd component, predominant both in its intensity and frequency of recognition by human IgE antibodies, was identified as the major allergen of C. arizonica. Four of the six IgE-binding components, including the major allergen, seem to be glycoproteins, as confirmed by the lectin blotting analysis. The extract produced inhouse was used to set up an immunoenzymatic test to evaluate the specific IgE binding in a panel of sera from 33 immunotherapy-free subjects who were monosensitized to cypress pollen. The percent of positivity obtained was much higher than that reported in the literature for commercial immunoassays.
