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Steven J Melnick - One of the best experts on this subject based on the ideXlab platform.
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therapeutic effect of supercritical co2 extracts of Curcuma species with cancer drugs in rhabdomyosarcoma cell lines
Phytotherapy Research, 2015Co-Authors: Cheppail Ramachandran, Enrique Escalon, Ivonne V Lollett, Karlw Quirin, Steven J MelnickAbstract:Synergistic effect of supercritical CO2 extracts of Curcuma species with conventional chemotherapeutic drugs was investigated in human alveolar (SJRH30) and embryonal (RD) rhabdomyosarcoma cell lines. The Curcuma Amada (mango ginger) (CA) extract showed the highest levels of cytotoxicity with inhibitory concentration IC50 values of 7.133 µg/ml and 7.501 µg/ml for SJRH30 and RD cell lines, respectively, as compared with Curcuma longa (turmeric) and Curcuma xanthorrhiza (Javanese turmeric) extracts. CA showed synergistic cytotoxic effects with vinblastine (VBL) and cyclophosphamide (CP) as indicated by the combination index values of <1 for VBL + CA, CP + CA, and VBL + CP + CA combinations in both embryonal and alveolar rhabdomyosarcomas. When lower doses of CA (0.1-0.2 µg/ml) were combined with cancer drugs like CP and VBL, caspase-3 activity increased significantly compared with individual agents and correlated with the percentage of apoptotic cells. CA in combination with VBL and CP induced a higher percentage of apoptosis than single agents in both cell lines. CA also modulated the expression of genes associated with intrinsic pathway of apoptosis (Bcl-2, Bax, Bak, and p53) and also inhibited the expression of genes associated with inflammation such as COX-2 and NF-κB. Xenograft studies with SJRH30 tumors in nude mice showed that CA treatment inhibited tumor growth rate with and without VBL and increased the survival rate significantly. These results suggest that CA can be evaluated further as an adjuvant with cancer drugs for the treatment of rhabdomyosarcoma patients. Copyright © 2015 John Wiley & Sons, Ltd.
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tr 02inhibition of akt signaling and tumor growth by supercritical co2 extract of mango ginger Curcuma Amada roxb in human glioblastoma cell line in vitro and in nude mice xenografts
Neuro-oncology, 2015Co-Authors: Cheppail Ramachandran, Gilda Portalatin, Karlwerner Quirin, Enrique Escalon, Ziad Khatib, Steven J MelnickAbstract:Mango ginger (Curcuma Amada Roxb.), a tropical medicinal plant and spice from Asia, has not been well-investigated for its anticancer properties unlike turmeric (Curcuma longa L.) or the active ingredient curcumin. The inhibitory effect of supercritical CO2 extract of mango ginger (CA) on AKT signaling was investigated in U-87MG glioblastoma cells. CA was highly cytotoxic to glioblastoma cell line (IC50 = 4.92 ± 0.81 µg/ml) compared to mHypoE-N1 normal mouse hypothalamus cell line (IC50 = 40.57 ± 0.06 µg/ml). When CA was combined with irinotecan (IR), temozolomide or vincristine, CA showed synergistic cytotoxicity with combination index values of <1 in glioblastoma cells. CA inhibits AKT and AMPKα phosphorylation significantly in a dose-dependent manner. The cell migration, which is necessary for invasion and metastasis, was also inhibited by CA treatment with about 43% inhibition at 20 µg/ml concentration. Analysis of mRNA and protein expression of genes showed that CA modulates expression of genes associated with apoptosis (Bax, Bcl-2, Bcl-X, BNIP3, caspase-3, mutant p53, and p21), cell proliferation (Ki67) and angiogenesis (VEGF). Additionally, HSP90 and AMPKα which interact with the AKT signaling pathway were down regulated by CA treatment. Analysis of tumor growth inhibition in nude mice xenografts transplanted with U-87MG glioblastoma cells showed that CA treatment has reduced tumor growth rate by 40.2%, IR by 23.7% and CA + IR by 99.5%. Both CA and CA + IR treated xenografts showed better survival rate than IR and control (saline) groups (*p < 0.0328). These results indicated the molecular targets underlying the anticancer effect of CA in human glioblastoma cells and the potential use of CA as an adjuvant for therapeutic management of glioblastoma.
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anticancer potential and mechanism of action of mango ginger Curcuma Amada roxb supercritical co2 extract in human glioblastoma cells
Journal of Evidence-Based Complementary & Alternative Medicine, 2015Co-Authors: Cheppail Ramachandran, Karlwerner Quirin, Enrique Escalon, Ivonne V Lollett, Steven J MelnickAbstract:Mango ginger (Curcuma Amada Roxb.) is among the less-investigated species of Curcuma for anticancer properties. We have investigated the anticancer potential and the mechanism of action of a supercritical CO2 extract of mango ginger (CA) in the U-87MG human glioblastoma cell line. CA demonstrated higher cytotoxicity than temozolomide, etoposide, curcumin, and turmeric force with IC50, IC75, and IC90 values of 4.92 μg/mL, 12.87 μg/mL, and 21.30 μg/mL, respectively. Inhibitory concentration values of CA for normal embryonic mouse hypothalamus cell line (mHypoE-N1) is significantly higher than glioblastoma cell line, indicating the specificity of CA against brain tumor cells. CompuSyn analysis indicates that CA acts synergistically with temozolomide and etoposide for the cytotoxicity with combination index values of <1. CA treatment also induces apoptosis in glioblastoma cells in a dose-dependent manner and downregulates genes associated with apoptosis, cell proliferation, telomerase activity, oncogenesis, a...
Cheppail Ramachandran - One of the best experts on this subject based on the ideXlab platform.
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therapeutic effect of supercritical co2 extracts of Curcuma species with cancer drugs in rhabdomyosarcoma cell lines
Phytotherapy Research, 2015Co-Authors: Cheppail Ramachandran, Enrique Escalon, Ivonne V Lollett, Karlw Quirin, Steven J MelnickAbstract:Synergistic effect of supercritical CO2 extracts of Curcuma species with conventional chemotherapeutic drugs was investigated in human alveolar (SJRH30) and embryonal (RD) rhabdomyosarcoma cell lines. The Curcuma Amada (mango ginger) (CA) extract showed the highest levels of cytotoxicity with inhibitory concentration IC50 values of 7.133 µg/ml and 7.501 µg/ml for SJRH30 and RD cell lines, respectively, as compared with Curcuma longa (turmeric) and Curcuma xanthorrhiza (Javanese turmeric) extracts. CA showed synergistic cytotoxic effects with vinblastine (VBL) and cyclophosphamide (CP) as indicated by the combination index values of <1 for VBL + CA, CP + CA, and VBL + CP + CA combinations in both embryonal and alveolar rhabdomyosarcomas. When lower doses of CA (0.1-0.2 µg/ml) were combined with cancer drugs like CP and VBL, caspase-3 activity increased significantly compared with individual agents and correlated with the percentage of apoptotic cells. CA in combination with VBL and CP induced a higher percentage of apoptosis than single agents in both cell lines. CA also modulated the expression of genes associated with intrinsic pathway of apoptosis (Bcl-2, Bax, Bak, and p53) and also inhibited the expression of genes associated with inflammation such as COX-2 and NF-κB. Xenograft studies with SJRH30 tumors in nude mice showed that CA treatment inhibited tumor growth rate with and without VBL and increased the survival rate significantly. These results suggest that CA can be evaluated further as an adjuvant with cancer drugs for the treatment of rhabdomyosarcoma patients. Copyright © 2015 John Wiley & Sons, Ltd.
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tr 02inhibition of akt signaling and tumor growth by supercritical co2 extract of mango ginger Curcuma Amada roxb in human glioblastoma cell line in vitro and in nude mice xenografts
Neuro-oncology, 2015Co-Authors: Cheppail Ramachandran, Gilda Portalatin, Karlwerner Quirin, Enrique Escalon, Ziad Khatib, Steven J MelnickAbstract:Mango ginger (Curcuma Amada Roxb.), a tropical medicinal plant and spice from Asia, has not been well-investigated for its anticancer properties unlike turmeric (Curcuma longa L.) or the active ingredient curcumin. The inhibitory effect of supercritical CO2 extract of mango ginger (CA) on AKT signaling was investigated in U-87MG glioblastoma cells. CA was highly cytotoxic to glioblastoma cell line (IC50 = 4.92 ± 0.81 µg/ml) compared to mHypoE-N1 normal mouse hypothalamus cell line (IC50 = 40.57 ± 0.06 µg/ml). When CA was combined with irinotecan (IR), temozolomide or vincristine, CA showed synergistic cytotoxicity with combination index values of <1 in glioblastoma cells. CA inhibits AKT and AMPKα phosphorylation significantly in a dose-dependent manner. The cell migration, which is necessary for invasion and metastasis, was also inhibited by CA treatment with about 43% inhibition at 20 µg/ml concentration. Analysis of mRNA and protein expression of genes showed that CA modulates expression of genes associated with apoptosis (Bax, Bcl-2, Bcl-X, BNIP3, caspase-3, mutant p53, and p21), cell proliferation (Ki67) and angiogenesis (VEGF). Additionally, HSP90 and AMPKα which interact with the AKT signaling pathway were down regulated by CA treatment. Analysis of tumor growth inhibition in nude mice xenografts transplanted with U-87MG glioblastoma cells showed that CA treatment has reduced tumor growth rate by 40.2%, IR by 23.7% and CA + IR by 99.5%. Both CA and CA + IR treated xenografts showed better survival rate than IR and control (saline) groups (*p < 0.0328). These results indicated the molecular targets underlying the anticancer effect of CA in human glioblastoma cells and the potential use of CA as an adjuvant for therapeutic management of glioblastoma.
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anticancer potential and mechanism of action of mango ginger Curcuma Amada roxb supercritical co2 extract in human glioblastoma cells
Journal of Evidence-Based Complementary & Alternative Medicine, 2015Co-Authors: Cheppail Ramachandran, Karlwerner Quirin, Enrique Escalon, Ivonne V Lollett, Steven J MelnickAbstract:Mango ginger (Curcuma Amada Roxb.) is among the less-investigated species of Curcuma for anticancer properties. We have investigated the anticancer potential and the mechanism of action of a supercritical CO2 extract of mango ginger (CA) in the U-87MG human glioblastoma cell line. CA demonstrated higher cytotoxicity than temozolomide, etoposide, curcumin, and turmeric force with IC50, IC75, and IC90 values of 4.92 μg/mL, 12.87 μg/mL, and 21.30 μg/mL, respectively. Inhibitory concentration values of CA for normal embryonic mouse hypothalamus cell line (mHypoE-N1) is significantly higher than glioblastoma cell line, indicating the specificity of CA against brain tumor cells. CompuSyn analysis indicates that CA acts synergistically with temozolomide and etoposide for the cytotoxicity with combination index values of <1. CA treatment also induces apoptosis in glioblastoma cells in a dose-dependent manner and downregulates genes associated with apoptosis, cell proliferation, telomerase activity, oncogenesis, a...
Enrique Escalon - One of the best experts on this subject based on the ideXlab platform.
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therapeutic effect of supercritical co2 extracts of Curcuma species with cancer drugs in rhabdomyosarcoma cell lines
Phytotherapy Research, 2015Co-Authors: Cheppail Ramachandran, Enrique Escalon, Ivonne V Lollett, Karlw Quirin, Steven J MelnickAbstract:Synergistic effect of supercritical CO2 extracts of Curcuma species with conventional chemotherapeutic drugs was investigated in human alveolar (SJRH30) and embryonal (RD) rhabdomyosarcoma cell lines. The Curcuma Amada (mango ginger) (CA) extract showed the highest levels of cytotoxicity with inhibitory concentration IC50 values of 7.133 µg/ml and 7.501 µg/ml for SJRH30 and RD cell lines, respectively, as compared with Curcuma longa (turmeric) and Curcuma xanthorrhiza (Javanese turmeric) extracts. CA showed synergistic cytotoxic effects with vinblastine (VBL) and cyclophosphamide (CP) as indicated by the combination index values of <1 for VBL + CA, CP + CA, and VBL + CP + CA combinations in both embryonal and alveolar rhabdomyosarcomas. When lower doses of CA (0.1-0.2 µg/ml) were combined with cancer drugs like CP and VBL, caspase-3 activity increased significantly compared with individual agents and correlated with the percentage of apoptotic cells. CA in combination with VBL and CP induced a higher percentage of apoptosis than single agents in both cell lines. CA also modulated the expression of genes associated with intrinsic pathway of apoptosis (Bcl-2, Bax, Bak, and p53) and also inhibited the expression of genes associated with inflammation such as COX-2 and NF-κB. Xenograft studies with SJRH30 tumors in nude mice showed that CA treatment inhibited tumor growth rate with and without VBL and increased the survival rate significantly. These results suggest that CA can be evaluated further as an adjuvant with cancer drugs for the treatment of rhabdomyosarcoma patients. Copyright © 2015 John Wiley & Sons, Ltd.
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tr 02inhibition of akt signaling and tumor growth by supercritical co2 extract of mango ginger Curcuma Amada roxb in human glioblastoma cell line in vitro and in nude mice xenografts
Neuro-oncology, 2015Co-Authors: Cheppail Ramachandran, Gilda Portalatin, Karlwerner Quirin, Enrique Escalon, Ziad Khatib, Steven J MelnickAbstract:Mango ginger (Curcuma Amada Roxb.), a tropical medicinal plant and spice from Asia, has not been well-investigated for its anticancer properties unlike turmeric (Curcuma longa L.) or the active ingredient curcumin. The inhibitory effect of supercritical CO2 extract of mango ginger (CA) on AKT signaling was investigated in U-87MG glioblastoma cells. CA was highly cytotoxic to glioblastoma cell line (IC50 = 4.92 ± 0.81 µg/ml) compared to mHypoE-N1 normal mouse hypothalamus cell line (IC50 = 40.57 ± 0.06 µg/ml). When CA was combined with irinotecan (IR), temozolomide or vincristine, CA showed synergistic cytotoxicity with combination index values of <1 in glioblastoma cells. CA inhibits AKT and AMPKα phosphorylation significantly in a dose-dependent manner. The cell migration, which is necessary for invasion and metastasis, was also inhibited by CA treatment with about 43% inhibition at 20 µg/ml concentration. Analysis of mRNA and protein expression of genes showed that CA modulates expression of genes associated with apoptosis (Bax, Bcl-2, Bcl-X, BNIP3, caspase-3, mutant p53, and p21), cell proliferation (Ki67) and angiogenesis (VEGF). Additionally, HSP90 and AMPKα which interact with the AKT signaling pathway were down regulated by CA treatment. Analysis of tumor growth inhibition in nude mice xenografts transplanted with U-87MG glioblastoma cells showed that CA treatment has reduced tumor growth rate by 40.2%, IR by 23.7% and CA + IR by 99.5%. Both CA and CA + IR treated xenografts showed better survival rate than IR and control (saline) groups (*p < 0.0328). These results indicated the molecular targets underlying the anticancer effect of CA in human glioblastoma cells and the potential use of CA as an adjuvant for therapeutic management of glioblastoma.
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anticancer potential and mechanism of action of mango ginger Curcuma Amada roxb supercritical co2 extract in human glioblastoma cells
Journal of Evidence-Based Complementary & Alternative Medicine, 2015Co-Authors: Cheppail Ramachandran, Karlwerner Quirin, Enrique Escalon, Ivonne V Lollett, Steven J MelnickAbstract:Mango ginger (Curcuma Amada Roxb.) is among the less-investigated species of Curcuma for anticancer properties. We have investigated the anticancer potential and the mechanism of action of a supercritical CO2 extract of mango ginger (CA) in the U-87MG human glioblastoma cell line. CA demonstrated higher cytotoxicity than temozolomide, etoposide, curcumin, and turmeric force with IC50, IC75, and IC90 values of 4.92 μg/mL, 12.87 μg/mL, and 21.30 μg/mL, respectively. Inhibitory concentration values of CA for normal embryonic mouse hypothalamus cell line (mHypoE-N1) is significantly higher than glioblastoma cell line, indicating the specificity of CA against brain tumor cells. CompuSyn analysis indicates that CA acts synergistically with temozolomide and etoposide for the cytotoxicity with combination index values of <1. CA treatment also induces apoptosis in glioblastoma cells in a dose-dependent manner and downregulates genes associated with apoptosis, cell proliferation, telomerase activity, oncogenesis, a...
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Anticancer Potential and Mechanism of Action of Mango Ginger ( Roxb.) Supercritical CO Extract in Human Glioblastoma Cells
'SAGE Publications', 2015Co-Authors: Cheppail Ramachandran Phd Mba, Karlwerner Quirin, Enrique Escalon, Steven Melnick J. PhdAbstract:Mango ginger ( Curcuma Amada Roxb.) is among the less-investigated species of Curcuma for anticancer properties. We have investigated the anticancer potential and the mechanism of action of a supercritical CO 2 extract of mango ginger (CA) in the U-87MG human glioblastoma cell line. CA demonstrated higher cytotoxicity than temozolomide, etoposide, curcumin, and turmeric force with IC 50 , IC 75 , and IC 90 values of 4.92 μg/mL, 12.87 μg/mL, and 21.30 μg/mL, respectively. Inhibitory concentration values of CA for normal embryonic mouse hypothalamus cell line (mHypoE-N1) is significantly higher than glioblastoma cell line, indicating the specificity of CA against brain tumor cells. CompuSyn analysis indicates that CA acts synergistically with temozolomide and etoposide for the cytotoxicity with combination index values of
Kazuo N Watanabe - One of the best experts on this subject based on the ideXlab platform.
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Environmentally safe in vitro regeneration protocol for
2011Co-Authors: Ahmad Jatoi, Kazuo N WatanabeAbstract:This study is a pioneer report on the development of an environmentally safe in vitro regeneration protocol for Curcuma, Kaempferia and Zingiber. The germplasm of the species was collected from Myanmar, a Southeast Asian country, rich in unexplored Zingiberaceae genetic resources. Rhizome buds were directly regenerated on the Murashige and Skoog medium containing a growth regulator, 6-benzyladenine and a commercial fungicide, Benlate (50 % of Benomyl). The pre-treatment protocol did not contain HgCl2, a toxic pollutant for Curcuma Amada, Curcuma longa, Zingiber barbatum and Kaempferia galanga. Plantlets were regenerated from the buds without any intervention of the callus phase. The contamination free survival of the bud explants from Curcuma, Zingiber and Kaempferia was more than 75, 57 and 53%, respectively. Buds from immature rhizomes were difficult to regenerate on the media, as well as resulted in higher contamination percentages while the buds from mature rhizomes efficiently regenerated with very few contamination percentages. The contamination was in the range of 0 to 39 % among the different accessions. This was also the first report of direct in vitro regeneration of plantlets from Z. barbatum bud explants. The protocol was cost-beneficial, time saving and effective for the conservation of Zingiberaceae genetic resources
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phytochemical pharmacological and ethnobotanical studies in mango ginger Curcuma Amada roxb zingiberaceae
Phytotherapy Research, 2007Co-Authors: Shakeel Ahmad Jatoi, Akira Kikuchi, Syed Amir Gilani, Kazuo N WatanabeAbstract:Curcuma Amada Roxb. is an important species known as mango ginger due to its characteristic raw-mango aroma. It has a long history of traditional uses ranging from folk medicine to several culinary preparations. The phytochemical, pharmacological and ethnobotanical studies of C. Amada are reviewed. The rhizome is rich in essential oils, and more than 130 chemical constituents with biomedical significance have been isolated from it. Its antibacterial, insecticidal, antifungal and antioxidant properties have been investigated. The conservation of indigenous knowledge by proper documentation is suggested. The chemotaxonomy, allelopathy and genetic diversity of C. Amada have not yet been explored, and many such studies are possible. This review was compiled to provide consolidated information covering different aspects of the plant, to provide a basis on which to plan future studies and to promote sustainable use of C. Amada.
Kumari Bai - One of the best experts on this subject based on the ideXlab platform.
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ANALGESIC ACTIVITY OF AQUEOUS EXTRACT OF Curcuma Amada (MANGO - GINGER) IN MALE ALBINO WISTAR RATS
Level Up Business Center, 2015Co-Authors: Kumari BaiAbstract:BACKGROUND: Mango ginger ( Curcuma Amada Roxb.) has morphological resemblance with ginger, but imparts mango flavour. According to Ayurveda and Unani medicinal systems , the biological activities include antioxidant, antibacterial, antifungal, anti - inflammatory, antiallergic, CNS depressant and analgesic activity. Hence Curcuma Amada aqueous extract for analgesic activity was evaluated in pain animal models. Pain is a most common complaint of many medical conditions, and pain control is one of t he most important therapeutic priorities. Curcuma Amada suppresses the inflammatory mediators associated with pain. However there is no scientific data suggestive of its analgesic activity. Hence this study was carried out to evaluate its role in central a nd peripheral models of pain. OBJECTIVE: To Evaluate rhizomes of Curcuma Amada for analgesic activity in male albino wistar rats . MATERIALS AND METHODS: Albino rats, the proven models for analgesic studies. They were obtained from the animal house of DR.B. R. Ambedkar Medical College. Animals were maintained as per CPCSEA guidelines . The aqueous extract of Curcuma Amada was used.4x2 groups of 6 Rats were used to ensure that results obtained were statistically significant using ANOVA test. Analgesic activity was assessed with the help of following screening methods . Acetic Acid Writhing Method using Acetic Acid . Tail Flick Method using the Analgesiometer . Tail Immersion Method using Hot Water (55 0 C) . Hot Plate method using Hot Plate . RESULT S: Aqueous extract of Curcuma Amada significantly suppressed the 1% acetic acid induced writhing response in rats when compared to control group (Gum acacia). In Tail flick test and Hot plate test Curcuma Amada increases the latency period of pain (reaction time). In Tail im mersion test the test drug significantly (P < 0.001) reduces pain at 30 min when compared to control group at 60 min of oral administration. CONCLUSION : The present findings indicate that Curcuma Amada showed significant analgesic activity may be via perip heral as well as central mechanisms
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AN EXPERIMENTAL COMPARATIVE STUDY OF ANALGESIC ACTIVITY OF Curcuma: Amada (MANGO - GINGER) WITH CONVENTIONAL NSAID ASPIRIN IN MALE ALBINO WISTAR RATS
Level Up Business Center, 2015Co-Authors: Kumari BaiAbstract:BACKGROUND: Mango ginger ( Curcuma Amada Roxb.) belongs to Zingiberaceae family has biological activities include antioxidant, antibacterial, antifungal, anti - inflammatory, antiallergic, CNS depressant and analgesic activity. The major chemical components include starch, phenolic acids, volatile oils, curcuminoids and terpenoids like difurocumenonol, Amadannulen and Amadaldehyde. Pain is often the first indication of disease or injury and a major symptom in many clinical conditions and can significantly interferes with a person’s quality of life and general functioning. The standard and test drugs suppress the inflammatory mediators associated with pain. This article brings out the analgesic activity of C. Amada in comparison with aspirin. Therefore aqueous extract of C. Amada was evaluated for analgesic activity in animal models of pain. OBJECTIVES: 1. To evaluate rhizomes of Curcuma Amada for analgesic activity in male albino wistar rats and to compare the analgesic activity with aspirin . 2. To Evaluate if combination of Curcuma Amada with aspirin is synergistic . MATERIALS AND METHODS: Albino rats are the proven models for analgesic studies. They were obtained from the animal house of DR.B. R. Ambedkar Medical College. Animals were maintained as per CPCSEA guidelines .The aqueous extract of Curcuma Amada was used. Aspirin (100mg/kg) was used as the standard analgesic drug. 4x4 groups of 6 Rats were used to ensure that results obtained were statistically significant using ANOVA test. Analgesic activity will be assessed with the help of following screening methods Acetic Acid Writhing Method using Acetic Acid, Tail Flick Method using the Analgesiometer, Tail Immersion Method using Hot Water (55 0 C) , Hot Plate method using Hot Plate . RESULTS : Aqueous extract of Curcuma Amada significantly suppressed the 1% acetic acid induced writhing response in rats when compared to standard drug aspirin. In the Tail flick and Hot plate test Curcuma Amada increases the latency period of pain ( R eaction time). In the Tail immersion test the test drug significantly (P
