The Experts below are selected from a list of 291 Experts worldwide ranked by ideXlab platform
Niels Fertig - One of the best experts on this subject based on the ideXlab platform.
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Characterization of iPS Derived Cardiomyocytes in Voltage Clamp and Current Clamp by Automated Patch Clamp
Biophysical Journal, 2017Co-Authors: Andrea Bruggemann, Claudia Haarmann, Michael George, Markus Rapedius, Tom A. Goetze, Ilka Rinke, Niels FertigAbstract:In recent years, human stem cell-derived cardiomyocytes have proven to recapitulate key features of human cardiac electrophysiology in vitro. Furthermore, it has become apparent that the intact ensemble of cardiac ion channels is necessary to determine proarrhythmic effects reliably. Hence, due to their increasing availability, stem cell-derived cardiomyocytes have become the preferred choice of cardiac cells.This poster summarizes the promises and challenges of combining iPS derived cardiac myocytes with automated patch Clamp. Features like high throughput, temperature control, easy internal solution exchange and full automation make planar patch Clamp a desired method for characterizing iPS derived cardiomyocytes.One of the biggest challenges of planar patch Clamp in this context is the fact that individual cells cannot be chosen, but cells will be selected randomly. In addition cells have to be harvested before the application to the patch Clamp chip and cannot be patched as adherent cells.With this poster, we show our progress on these challenges. Two automated patch Clamp platforms, the Patchliner, as a medium throughput, and the SyncroPatch 384PE, as a high throughput device, were used for this study.Pharmacological measurements in voltage Clamp as well as Current Clamp will be shown also under physiological temperatures and perforated patch.
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state of the art automated patch Clamp devices heat activation action potentials and high throughput in ion channel screening
Frontiers in Pharmacology, 2011Co-Authors: Sonja Stoelzle, Claudia Haarmann, Andrea Bruggemann, Michael George, Ralf Kettenhofen, Alison Obergrussberger, Niels FertigAbstract:Ion channels are essential in a wide range of cellular functions and their malfunction underlies many disease states making them important targets in drug discovery. The availability of standardized cell lines expressing ion channels of interest lead to the development of diverse automated patch Clamp (APC) systems with high-throughput capabilities. These systems are now available for drug screening, but there are limitations in the application range. However, further development of existing devices and introduction of new systems widen the range of possible experiments and increase throughput. The addition of well controlled and fast solution exchange, temperature control and the availability of the Current Clamp mode are required to analyze standard cell lines and excitable cells such as stem cell-derived cardiomyocytes in a more physiologically relevant environment. Here we describe two systems with different areas of applications that meet the needs of drug discovery researchers and basic researchers alike. The here utilized medium throughput APC device is a planar patch Clamp system capable of recording up to eight cells simultaneously. Features such as temperature control and recordings in the Current Clamp mode are described here. Standard cell lines and excitable cells such as stem cell-derived cardiomyocytes have been used in the voltage Clamp and Current Clamp modes with the view to finding new drug candidates and safety testing methods in a more physiologically relevant environment. The high-throughput system used here is a planar patch Clamp screening platform capable of recording from 96 cells in parallel and offers a throughput of 5000 data points per day. Full dose response curves can be acquired from individual cells reducing the cost per data point. The data provided reveals the suitability and relevance of both APC platforms for drug discovery, ion channel research, and safety testing.
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automated patch Clamp with Current Clamp action potential recordings from stem cell derived cardiomyocytes
Biophysical Journal, 2011Co-Authors: Sonja Stoelzle, Alison Haythornthwaite, Claudia Haarmann, Cecilia Farre, Rodolfo J Haedo, Andrea Bruggemann, Michael George, Niels FertigAbstract:Automated planar patch Clamp devices, with their higher throughput and high data information content, are finding their place in the market for making high quality electrophysiological measurements.Ease of use and higher data throughput make these devices ideal tools for ion channel screening and safety testing. The ability to record from stem cell derived cardiomyocytes in both voltage and Current Clamp modes on an automated patch Clamp platform is an important advancement in ion channel screening and safety testing. Using a planar patch Clamp workstation, Currents mediated by K+, Na+ and Ca2+ channels could be recorded from stem cell derived cardiomyocytes in the voltage Clamp mode. In the Current Clamp mode, action potentials could be recorded for the first time on a planar patch Clamp device. The built-in temperature control allowed for pharmacology on action potentials (see figure) both at room temperature and 35°.The use of stem cell derived cardiomyocytes in safety testing is becoming increasingly important. The ability to test compounds on ion channels in both voltage and Current Clamp modes, as well as at different temperatures, may be crucial for future safety testing.View Large Image | View Hi-Res Image | Download PowerPoint Slide
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Excitement Over Automated Patch Clamp: Action Potentials from Cardiac Myocytes
Biophysical Journal, 2010Co-Authors: Sonja Stoelzle, Alison Haythornthwaite, Claudia Haarmann, Cecilia Farre, Andrea Bruggemann, Michael George, David Guinot, Ralf Kettenhofen, Niels FertigAbstract:The use of cardiac myocytes is becoming increasingly important for drug safety testing. Unique features of certain planar patch Clamp workstations, coupled with ease-of-use and higher data throughput, make these devices ideal tools for ion channel screening and safety testing. Using stem cell derived cardiac myocytes, recordings could be made not only in the voltage-Clamp mode but also in the Current-Clamp mode on a planar patch Clamp workstation. This demonstrates for the first time parallel Current-Clamp recordings on a planar patch Clamp workstation. Ion channels important in drug discovery, such as hERG and voltage-gated Na+, Ca2+ and K+ channels in the voltage-Clamp mode from stem cell derived cardiac myocytes will be shown. In addition, action potential recordings in the Current-Clamp mode at 35°C, and modulation of the action potentials by hERG active compounds, will also be shown.View Large Image | View Hi-Res Image | Download PowerPoint Slide
Andrea Bruggemann - One of the best experts on this subject based on the ideXlab platform.
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Characterization of iPS Derived Cardiomyocytes in Voltage Clamp and Current Clamp by Automated Patch Clamp
Biophysical Journal, 2017Co-Authors: Andrea Bruggemann, Claudia Haarmann, Michael George, Markus Rapedius, Tom A. Goetze, Ilka Rinke, Niels FertigAbstract:In recent years, human stem cell-derived cardiomyocytes have proven to recapitulate key features of human cardiac electrophysiology in vitro. Furthermore, it has become apparent that the intact ensemble of cardiac ion channels is necessary to determine proarrhythmic effects reliably. Hence, due to their increasing availability, stem cell-derived cardiomyocytes have become the preferred choice of cardiac cells.This poster summarizes the promises and challenges of combining iPS derived cardiac myocytes with automated patch Clamp. Features like high throughput, temperature control, easy internal solution exchange and full automation make planar patch Clamp a desired method for characterizing iPS derived cardiomyocytes.One of the biggest challenges of planar patch Clamp in this context is the fact that individual cells cannot be chosen, but cells will be selected randomly. In addition cells have to be harvested before the application to the patch Clamp chip and cannot be patched as adherent cells.With this poster, we show our progress on these challenges. Two automated patch Clamp platforms, the Patchliner, as a medium throughput, and the SyncroPatch 384PE, as a high throughput device, were used for this study.Pharmacological measurements in voltage Clamp as well as Current Clamp will be shown also under physiological temperatures and perforated patch.
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state of the art automated patch Clamp devices heat activation action potentials and high throughput in ion channel screening
Frontiers in Pharmacology, 2011Co-Authors: Sonja Stoelzle, Claudia Haarmann, Andrea Bruggemann, Michael George, Ralf Kettenhofen, Alison Obergrussberger, Niels FertigAbstract:Ion channels are essential in a wide range of cellular functions and their malfunction underlies many disease states making them important targets in drug discovery. The availability of standardized cell lines expressing ion channels of interest lead to the development of diverse automated patch Clamp (APC) systems with high-throughput capabilities. These systems are now available for drug screening, but there are limitations in the application range. However, further development of existing devices and introduction of new systems widen the range of possible experiments and increase throughput. The addition of well controlled and fast solution exchange, temperature control and the availability of the Current Clamp mode are required to analyze standard cell lines and excitable cells such as stem cell-derived cardiomyocytes in a more physiologically relevant environment. Here we describe two systems with different areas of applications that meet the needs of drug discovery researchers and basic researchers alike. The here utilized medium throughput APC device is a planar patch Clamp system capable of recording up to eight cells simultaneously. Features such as temperature control and recordings in the Current Clamp mode are described here. Standard cell lines and excitable cells such as stem cell-derived cardiomyocytes have been used in the voltage Clamp and Current Clamp modes with the view to finding new drug candidates and safety testing methods in a more physiologically relevant environment. The high-throughput system used here is a planar patch Clamp screening platform capable of recording from 96 cells in parallel and offers a throughput of 5000 data points per day. Full dose response curves can be acquired from individual cells reducing the cost per data point. The data provided reveals the suitability and relevance of both APC platforms for drug discovery, ion channel research, and safety testing.
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automated patch Clamp with Current Clamp action potential recordings from stem cell derived cardiomyocytes
Biophysical Journal, 2011Co-Authors: Sonja Stoelzle, Alison Haythornthwaite, Claudia Haarmann, Cecilia Farre, Rodolfo J Haedo, Andrea Bruggemann, Michael George, Niels FertigAbstract:Automated planar patch Clamp devices, with their higher throughput and high data information content, are finding their place in the market for making high quality electrophysiological measurements.Ease of use and higher data throughput make these devices ideal tools for ion channel screening and safety testing. The ability to record from stem cell derived cardiomyocytes in both voltage and Current Clamp modes on an automated patch Clamp platform is an important advancement in ion channel screening and safety testing. Using a planar patch Clamp workstation, Currents mediated by K+, Na+ and Ca2+ channels could be recorded from stem cell derived cardiomyocytes in the voltage Clamp mode. In the Current Clamp mode, action potentials could be recorded for the first time on a planar patch Clamp device. The built-in temperature control allowed for pharmacology on action potentials (see figure) both at room temperature and 35°.The use of stem cell derived cardiomyocytes in safety testing is becoming increasingly important. The ability to test compounds on ion channels in both voltage and Current Clamp modes, as well as at different temperatures, may be crucial for future safety testing.View Large Image | View Hi-Res Image | Download PowerPoint Slide
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Excitement Over Automated Patch Clamp: Action Potentials from Cardiac Myocytes
Biophysical Journal, 2010Co-Authors: Sonja Stoelzle, Alison Haythornthwaite, Claudia Haarmann, Cecilia Farre, Andrea Bruggemann, Michael George, David Guinot, Ralf Kettenhofen, Niels FertigAbstract:The use of cardiac myocytes is becoming increasingly important for drug safety testing. Unique features of certain planar patch Clamp workstations, coupled with ease-of-use and higher data throughput, make these devices ideal tools for ion channel screening and safety testing. Using stem cell derived cardiac myocytes, recordings could be made not only in the voltage-Clamp mode but also in the Current-Clamp mode on a planar patch Clamp workstation. This demonstrates for the first time parallel Current-Clamp recordings on a planar patch Clamp workstation. Ion channels important in drug discovery, such as hERG and voltage-gated Na+, Ca2+ and K+ channels in the voltage-Clamp mode from stem cell derived cardiac myocytes will be shown. In addition, action potential recordings in the Current-Clamp mode at 35°C, and modulation of the action potentials by hERG active compounds, will also be shown.View Large Image | View Hi-Res Image | Download PowerPoint Slide
Claudia Haarmann - One of the best experts on this subject based on the ideXlab platform.
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Characterization of iPS Derived Cardiomyocytes in Voltage Clamp and Current Clamp by Automated Patch Clamp
Biophysical Journal, 2017Co-Authors: Andrea Bruggemann, Claudia Haarmann, Michael George, Markus Rapedius, Tom A. Goetze, Ilka Rinke, Niels FertigAbstract:In recent years, human stem cell-derived cardiomyocytes have proven to recapitulate key features of human cardiac electrophysiology in vitro. Furthermore, it has become apparent that the intact ensemble of cardiac ion channels is necessary to determine proarrhythmic effects reliably. Hence, due to their increasing availability, stem cell-derived cardiomyocytes have become the preferred choice of cardiac cells.This poster summarizes the promises and challenges of combining iPS derived cardiac myocytes with automated patch Clamp. Features like high throughput, temperature control, easy internal solution exchange and full automation make planar patch Clamp a desired method for characterizing iPS derived cardiomyocytes.One of the biggest challenges of planar patch Clamp in this context is the fact that individual cells cannot be chosen, but cells will be selected randomly. In addition cells have to be harvested before the application to the patch Clamp chip and cannot be patched as adherent cells.With this poster, we show our progress on these challenges. Two automated patch Clamp platforms, the Patchliner, as a medium throughput, and the SyncroPatch 384PE, as a high throughput device, were used for this study.Pharmacological measurements in voltage Clamp as well as Current Clamp will be shown also under physiological temperatures and perforated patch.
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state of the art automated patch Clamp devices heat activation action potentials and high throughput in ion channel screening
Frontiers in Pharmacology, 2011Co-Authors: Sonja Stoelzle, Claudia Haarmann, Andrea Bruggemann, Michael George, Ralf Kettenhofen, Alison Obergrussberger, Niels FertigAbstract:Ion channels are essential in a wide range of cellular functions and their malfunction underlies many disease states making them important targets in drug discovery. The availability of standardized cell lines expressing ion channels of interest lead to the development of diverse automated patch Clamp (APC) systems with high-throughput capabilities. These systems are now available for drug screening, but there are limitations in the application range. However, further development of existing devices and introduction of new systems widen the range of possible experiments and increase throughput. The addition of well controlled and fast solution exchange, temperature control and the availability of the Current Clamp mode are required to analyze standard cell lines and excitable cells such as stem cell-derived cardiomyocytes in a more physiologically relevant environment. Here we describe two systems with different areas of applications that meet the needs of drug discovery researchers and basic researchers alike. The here utilized medium throughput APC device is a planar patch Clamp system capable of recording up to eight cells simultaneously. Features such as temperature control and recordings in the Current Clamp mode are described here. Standard cell lines and excitable cells such as stem cell-derived cardiomyocytes have been used in the voltage Clamp and Current Clamp modes with the view to finding new drug candidates and safety testing methods in a more physiologically relevant environment. The high-throughput system used here is a planar patch Clamp screening platform capable of recording from 96 cells in parallel and offers a throughput of 5000 data points per day. Full dose response curves can be acquired from individual cells reducing the cost per data point. The data provided reveals the suitability and relevance of both APC platforms for drug discovery, ion channel research, and safety testing.
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automated patch Clamp with Current Clamp action potential recordings from stem cell derived cardiomyocytes
Biophysical Journal, 2011Co-Authors: Sonja Stoelzle, Alison Haythornthwaite, Claudia Haarmann, Cecilia Farre, Rodolfo J Haedo, Andrea Bruggemann, Michael George, Niels FertigAbstract:Automated planar patch Clamp devices, with their higher throughput and high data information content, are finding their place in the market for making high quality electrophysiological measurements.Ease of use and higher data throughput make these devices ideal tools for ion channel screening and safety testing. The ability to record from stem cell derived cardiomyocytes in both voltage and Current Clamp modes on an automated patch Clamp platform is an important advancement in ion channel screening and safety testing. Using a planar patch Clamp workstation, Currents mediated by K+, Na+ and Ca2+ channels could be recorded from stem cell derived cardiomyocytes in the voltage Clamp mode. In the Current Clamp mode, action potentials could be recorded for the first time on a planar patch Clamp device. The built-in temperature control allowed for pharmacology on action potentials (see figure) both at room temperature and 35°.The use of stem cell derived cardiomyocytes in safety testing is becoming increasingly important. The ability to test compounds on ion channels in both voltage and Current Clamp modes, as well as at different temperatures, may be crucial for future safety testing.View Large Image | View Hi-Res Image | Download PowerPoint Slide
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Excitement Over Automated Patch Clamp: Action Potentials from Cardiac Myocytes
Biophysical Journal, 2010Co-Authors: Sonja Stoelzle, Alison Haythornthwaite, Claudia Haarmann, Cecilia Farre, Andrea Bruggemann, Michael George, David Guinot, Ralf Kettenhofen, Niels FertigAbstract:The use of cardiac myocytes is becoming increasingly important for drug safety testing. Unique features of certain planar patch Clamp workstations, coupled with ease-of-use and higher data throughput, make these devices ideal tools for ion channel screening and safety testing. Using stem cell derived cardiac myocytes, recordings could be made not only in the voltage-Clamp mode but also in the Current-Clamp mode on a planar patch Clamp workstation. This demonstrates for the first time parallel Current-Clamp recordings on a planar patch Clamp workstation. Ion channels important in drug discovery, such as hERG and voltage-gated Na+, Ca2+ and K+ channels in the voltage-Clamp mode from stem cell derived cardiac myocytes will be shown. In addition, action potential recordings in the Current-Clamp mode at 35°C, and modulation of the action potentials by hERG active compounds, will also be shown.View Large Image | View Hi-Res Image | Download PowerPoint Slide
Michael George - One of the best experts on this subject based on the ideXlab platform.
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Characterization of iPS Derived Cardiomyocytes in Voltage Clamp and Current Clamp by Automated Patch Clamp
Biophysical Journal, 2017Co-Authors: Andrea Bruggemann, Claudia Haarmann, Michael George, Markus Rapedius, Tom A. Goetze, Ilka Rinke, Niels FertigAbstract:In recent years, human stem cell-derived cardiomyocytes have proven to recapitulate key features of human cardiac electrophysiology in vitro. Furthermore, it has become apparent that the intact ensemble of cardiac ion channels is necessary to determine proarrhythmic effects reliably. Hence, due to their increasing availability, stem cell-derived cardiomyocytes have become the preferred choice of cardiac cells.This poster summarizes the promises and challenges of combining iPS derived cardiac myocytes with automated patch Clamp. Features like high throughput, temperature control, easy internal solution exchange and full automation make planar patch Clamp a desired method for characterizing iPS derived cardiomyocytes.One of the biggest challenges of planar patch Clamp in this context is the fact that individual cells cannot be chosen, but cells will be selected randomly. In addition cells have to be harvested before the application to the patch Clamp chip and cannot be patched as adherent cells.With this poster, we show our progress on these challenges. Two automated patch Clamp platforms, the Patchliner, as a medium throughput, and the SyncroPatch 384PE, as a high throughput device, were used for this study.Pharmacological measurements in voltage Clamp as well as Current Clamp will be shown also under physiological temperatures and perforated patch.
-
state of the art automated patch Clamp devices heat activation action potentials and high throughput in ion channel screening
Frontiers in Pharmacology, 2011Co-Authors: Sonja Stoelzle, Claudia Haarmann, Andrea Bruggemann, Michael George, Ralf Kettenhofen, Alison Obergrussberger, Niels FertigAbstract:Ion channels are essential in a wide range of cellular functions and their malfunction underlies many disease states making them important targets in drug discovery. The availability of standardized cell lines expressing ion channels of interest lead to the development of diverse automated patch Clamp (APC) systems with high-throughput capabilities. These systems are now available for drug screening, but there are limitations in the application range. However, further development of existing devices and introduction of new systems widen the range of possible experiments and increase throughput. The addition of well controlled and fast solution exchange, temperature control and the availability of the Current Clamp mode are required to analyze standard cell lines and excitable cells such as stem cell-derived cardiomyocytes in a more physiologically relevant environment. Here we describe two systems with different areas of applications that meet the needs of drug discovery researchers and basic researchers alike. The here utilized medium throughput APC device is a planar patch Clamp system capable of recording up to eight cells simultaneously. Features such as temperature control and recordings in the Current Clamp mode are described here. Standard cell lines and excitable cells such as stem cell-derived cardiomyocytes have been used in the voltage Clamp and Current Clamp modes with the view to finding new drug candidates and safety testing methods in a more physiologically relevant environment. The high-throughput system used here is a planar patch Clamp screening platform capable of recording from 96 cells in parallel and offers a throughput of 5000 data points per day. Full dose response curves can be acquired from individual cells reducing the cost per data point. The data provided reveals the suitability and relevance of both APC platforms for drug discovery, ion channel research, and safety testing.
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automated patch Clamp with Current Clamp action potential recordings from stem cell derived cardiomyocytes
Biophysical Journal, 2011Co-Authors: Sonja Stoelzle, Alison Haythornthwaite, Claudia Haarmann, Cecilia Farre, Rodolfo J Haedo, Andrea Bruggemann, Michael George, Niels FertigAbstract:Automated planar patch Clamp devices, with their higher throughput and high data information content, are finding their place in the market for making high quality electrophysiological measurements.Ease of use and higher data throughput make these devices ideal tools for ion channel screening and safety testing. The ability to record from stem cell derived cardiomyocytes in both voltage and Current Clamp modes on an automated patch Clamp platform is an important advancement in ion channel screening and safety testing. Using a planar patch Clamp workstation, Currents mediated by K+, Na+ and Ca2+ channels could be recorded from stem cell derived cardiomyocytes in the voltage Clamp mode. In the Current Clamp mode, action potentials could be recorded for the first time on a planar patch Clamp device. The built-in temperature control allowed for pharmacology on action potentials (see figure) both at room temperature and 35°.The use of stem cell derived cardiomyocytes in safety testing is becoming increasingly important. The ability to test compounds on ion channels in both voltage and Current Clamp modes, as well as at different temperatures, may be crucial for future safety testing.View Large Image | View Hi-Res Image | Download PowerPoint Slide
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Excitement Over Automated Patch Clamp: Action Potentials from Cardiac Myocytes
Biophysical Journal, 2010Co-Authors: Sonja Stoelzle, Alison Haythornthwaite, Claudia Haarmann, Cecilia Farre, Andrea Bruggemann, Michael George, David Guinot, Ralf Kettenhofen, Niels FertigAbstract:The use of cardiac myocytes is becoming increasingly important for drug safety testing. Unique features of certain planar patch Clamp workstations, coupled with ease-of-use and higher data throughput, make these devices ideal tools for ion channel screening and safety testing. Using stem cell derived cardiac myocytes, recordings could be made not only in the voltage-Clamp mode but also in the Current-Clamp mode on a planar patch Clamp workstation. This demonstrates for the first time parallel Current-Clamp recordings on a planar patch Clamp workstation. Ion channels important in drug discovery, such as hERG and voltage-gated Na+, Ca2+ and K+ channels in the voltage-Clamp mode from stem cell derived cardiac myocytes will be shown. In addition, action potential recordings in the Current-Clamp mode at 35°C, and modulation of the action potentials by hERG active compounds, will also be shown.View Large Image | View Hi-Res Image | Download PowerPoint Slide
Sonja Stoelzle - One of the best experts on this subject based on the ideXlab platform.
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state of the art automated patch Clamp devices heat activation action potentials and high throughput in ion channel screening
Frontiers in Pharmacology, 2011Co-Authors: Sonja Stoelzle, Claudia Haarmann, Andrea Bruggemann, Michael George, Ralf Kettenhofen, Alison Obergrussberger, Niels FertigAbstract:Ion channels are essential in a wide range of cellular functions and their malfunction underlies many disease states making them important targets in drug discovery. The availability of standardized cell lines expressing ion channels of interest lead to the development of diverse automated patch Clamp (APC) systems with high-throughput capabilities. These systems are now available for drug screening, but there are limitations in the application range. However, further development of existing devices and introduction of new systems widen the range of possible experiments and increase throughput. The addition of well controlled and fast solution exchange, temperature control and the availability of the Current Clamp mode are required to analyze standard cell lines and excitable cells such as stem cell-derived cardiomyocytes in a more physiologically relevant environment. Here we describe two systems with different areas of applications that meet the needs of drug discovery researchers and basic researchers alike. The here utilized medium throughput APC device is a planar patch Clamp system capable of recording up to eight cells simultaneously. Features such as temperature control and recordings in the Current Clamp mode are described here. Standard cell lines and excitable cells such as stem cell-derived cardiomyocytes have been used in the voltage Clamp and Current Clamp modes with the view to finding new drug candidates and safety testing methods in a more physiologically relevant environment. The high-throughput system used here is a planar patch Clamp screening platform capable of recording from 96 cells in parallel and offers a throughput of 5000 data points per day. Full dose response curves can be acquired from individual cells reducing the cost per data point. The data provided reveals the suitability and relevance of both APC platforms for drug discovery, ion channel research, and safety testing.
-
automated patch Clamp with Current Clamp action potential recordings from stem cell derived cardiomyocytes
Biophysical Journal, 2011Co-Authors: Sonja Stoelzle, Alison Haythornthwaite, Claudia Haarmann, Cecilia Farre, Rodolfo J Haedo, Andrea Bruggemann, Michael George, Niels FertigAbstract:Automated planar patch Clamp devices, with their higher throughput and high data information content, are finding their place in the market for making high quality electrophysiological measurements.Ease of use and higher data throughput make these devices ideal tools for ion channel screening and safety testing. The ability to record from stem cell derived cardiomyocytes in both voltage and Current Clamp modes on an automated patch Clamp platform is an important advancement in ion channel screening and safety testing. Using a planar patch Clamp workstation, Currents mediated by K+, Na+ and Ca2+ channels could be recorded from stem cell derived cardiomyocytes in the voltage Clamp mode. In the Current Clamp mode, action potentials could be recorded for the first time on a planar patch Clamp device. The built-in temperature control allowed for pharmacology on action potentials (see figure) both at room temperature and 35°.The use of stem cell derived cardiomyocytes in safety testing is becoming increasingly important. The ability to test compounds on ion channels in both voltage and Current Clamp modes, as well as at different temperatures, may be crucial for future safety testing.View Large Image | View Hi-Res Image | Download PowerPoint Slide
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Excitement Over Automated Patch Clamp: Action Potentials from Cardiac Myocytes
Biophysical Journal, 2010Co-Authors: Sonja Stoelzle, Alison Haythornthwaite, Claudia Haarmann, Cecilia Farre, Andrea Bruggemann, Michael George, David Guinot, Ralf Kettenhofen, Niels FertigAbstract:The use of cardiac myocytes is becoming increasingly important for drug safety testing. Unique features of certain planar patch Clamp workstations, coupled with ease-of-use and higher data throughput, make these devices ideal tools for ion channel screening and safety testing. Using stem cell derived cardiac myocytes, recordings could be made not only in the voltage-Clamp mode but also in the Current-Clamp mode on a planar patch Clamp workstation. This demonstrates for the first time parallel Current-Clamp recordings on a planar patch Clamp workstation. Ion channels important in drug discovery, such as hERG and voltage-gated Na+, Ca2+ and K+ channels in the voltage-Clamp mode from stem cell derived cardiac myocytes will be shown. In addition, action potential recordings in the Current-Clamp mode at 35°C, and modulation of the action potentials by hERG active compounds, will also be shown.View Large Image | View Hi-Res Image | Download PowerPoint Slide
