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Sirichai Adisakwattana - One of the best experts on this subject based on the ideXlab platform.
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Protective effect of Cyanidin against glucose- and methylglyoxal-induced protein glycation and oxidative DNA damage
International journal of biological macromolecules, 2016Co-Authors: Tanyawan Suantawee, Henrique Cheng, Sirichai AdisakwattanaAbstract:Cyanidin, a natural anthocyanin abundant in fruits and vegetables, has shown the health benefits due to its pharmacological properties. However, there was no evidence regarding anti-glycation activity of Cyanidin. The aim of the study was to investigate the inhibitory effect of Cyanidin on methylglyoxal (MG)- and glucose-induced protein glycation in bovine serum albumin (BSA) as well as oxidative DNA damage. Free radical scavenging activity and the MG-trapping ability of Cyanidin were also investigated. The results demonstrated that Cyanidin (0.125-1mM) significantly inhibited the formation of fluorescent and non-fluorescent AGEs in BSA/MG and BSA/glucose systems. There was a significantly improved protein thiol in BSA/MG and BSA/glucose when incubated with Cyanidin. Correspondingly, Cyanidin decreased the level of protein carbonyl content in BSA/glucose system. Moreover, Cyanidin (0.5-1mM) prevented lysine/MG-mediated oxidative DNA damage in the absence or presence of copper ion. The results demonstrated that Cyanidin showed the MG-trapping ability in a concentration-dependent manner. Cyanidin also reduced superoxide anion and hydroxyl radical generation in lysine/MG system. The mechanism by which Cyanidin inhibited protein glycation was the MG-trapping ability and the free radical scavenging activity. The present study suggests that Cyanidin might be a promising antiglycation agent for preventing or ameliorating AGEs-mediated diabetic complications.
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Cyanidin 3 rutinoside alleviates postprandial hyperglycemia and its synergism with acarbose by inhibition of intestinal α glucosidase
Journal of Clinical Biochemistry and Nutrition, 2011Co-Authors: Sirichai Adisakwattana, Sirintorn Yibchokanun, Piyawan Charoenlertkul, Natthakarn WongsasiripatAbstract:The inhibitory activity on intestinal α-glucosidase by Cyanidin-3-rutinoside was examined in vitro and in vivo. The IC50 values of Cyanidin-3-rutinoside against intestinal maltase, and sucrase were 2,323 ± 14.8 and 250.2 ± 8.1 µM, respectively. The kinetic analysis revealed that intestinal sucrase was inhibited by Cyanidin-3-rutinoside in a mixed-type manner. The synergistic inhibition also found in combination of Cyanidin-3-rutinoside with acarbose against intestinal maltase and sucrase. The oral administration of Cyanidin-3-rutinoside (100 and 300 mg/kg) plus maltose or sucrose to normal rats, postprandial plasma glucose was markedly suppressed at 30–90 min after loading. Furthermore, the normal rats treated with acarbose and Cyanidin-3-rutinoside (30 mg/kg) showed greater reduction of postprandial plasma glucose than the group treated with acarbose alone. These results suggest that Cyanidin-3-rutinoside retards absorption of carbohydrates by inhibition of α-glucosidase which may be useful as a potential inhibitor for prevention and treatment of diabetes mellitus.
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in vitro inhibitory effects of cyandin 3 rutinoside on pancreatic α amylase and its combined effect with acarbose
Molecules, 2011Co-Authors: Sarinya Akkarachiyasit, Sirintorn Yibchokanun, Sumrit Wacharasindhu, Sirichai AdisakwattanaAbstract:The inhibitory activity on pancreatic α-amylase by Cyanidin-3-rutinoside was examined in vitro. The IC50 value of Cyanidin-3-rutinoside against pancreatic α-amylase was 24.4 ± 0.1 μM. The kinetic analysis revealed that pancreatic α-amylase was inhibited by Cyanidin-3-rutinoside in a non-competitive manner. The additive inhibition of a combination of Cyanidin-3-rutinoside with acarbose against pancreatic α-amylase was also found. These results provide the first evidence for the effect of Cyanidin-3-rutinoside in a retarded absorption of carbohydrates by inhibition of pancreatic α-amylase which may be useful as a potential inhibitor for prevention and treatment of diabetes mellitus.
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inhibitory activities of Cyanidin and its glycosides and synergistic effect with acarbose against intestinal α glucosidase and pancreatic α amylase
International Journal of Molecular Sciences, 2010Co-Authors: Sarinya Akkarachiyasit, Piyawan Charoenlertkul, Sirintorn Yibchokanun, Sirichai AdisakwattanaAbstract:Cyanidin and its glycosides are naturally dietary pigments which have been indicated as promising candidates to have potential benefits to humans, especially in the prevention and treatment of diabetes mellitus. We investigated the structure activity relationships of Cyanidin and its glycosides to inhibit intestinal a-glucosidases and pancreatic a-amylase in vitro. The results found that Cyanidin and its glycosides are more specific inhibitors of intestinal sucrase than intestinal maltase. Cyanidin-3-galactoside and Cyanidin-3-glucoside were the most potent inhibitors against intestinal sucrase and pancreatic a-amylase with IC50 values of 0.50 ± 0.05 and 0.30 ± 0.01 mM, respectively. Our findings indicate that the structural difference between glucose and galactose at the 3-O-position of Cyanidin was an important factor for modulating the inhibition of intestinal sucrase and pancreatic a-amylase. The combination of cyandin-3-glucoside, Cyanidin-3-galactoside or Cyanidin-3,5-diglucosides with a low concentration of acarbose showed synergistic inhibition on intestinal maltase and sucrase. The synergistic inhibition was also found for a combination of Cyanidin or Cyanidin-3-glucoside with a low concentration of acarbose. The findings could provide a new insight into a use for the naturally occurring intestinal a-glucosidase and pancreatic a-amylase inhibitors for the prevention and treatment of diabetes and its complications.
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alpha glucosidase inhibitory activity of Cyanidin 3 galactoside and synergistic effect with acarbose
Journal of Enzyme Inhibition and Medicinal Chemistry, 2009Co-Authors: Sirichai Adisakwattana, Piyawan Charoenlertkul, Sirintorn YibchokanunAbstract:Cyanidin-3-galactoside, a natural anthocyanin, was investigated for its alpha-glucosidase inhibitory activity. The IC(50) value of Cyanidin-3-galactoside was 0.50 +/- 0.05 mM against intestinal sucrase. A low dose of Cyanidin-3-galactoside showed a synergistic inhibition on intestinal alpha-glucosidase (maltase and sucrase) when combined with acarbose. A kinetic analysis showed that Cyanidin-3-galactoside gave a mixed type inhibition against intestinal sucrase. The results indicated that Cyanidin-3-galactoside was an alpha-glucosidase inhibitor and could be used in combination with acarbose for treatment of diabetes.
Nobuji Nakatani - One of the best experts on this subject based on the ideXlab platform.
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stability of anthocyanins of sambucus canadensis and sambucus nigra
Journal of Agricultural and Food Chemistry, 1996Co-Authors: Osamu Inami, Itaru Tamura, Hiroe Kikuzaki, Nobuji NakataniAbstract:Elderberry anthocyanins (Sambucus nigra) including acylated derivatives (Sambucus canadensis) were studied for use as beverage pigments. Cyanidin 3-O-(6-O-E-p-coumaroyl-2-O-β-d-xylopyranosyl)-β-d-glucopyranoside-5-O-β-d-glucopyranoside from S. canadensis was more stable than Cyanidin 3-sambubioside from S. nigra. Acylation improved both heat and light stability, whereas glycosidation only stabilized anthocyanins in the presence of light. Cyanidin 3-(E)-p-coumaroyl-sambubioside-5-glucoside changed to three new anthocyanins under light irradiation. These three anthocyanins were isolated, and their structures were identified to be Cyanidin 3-O-(6-O-Z-p-coumaroyl-2-O-β-d-xylopyranosyl)-β-d-glucopyranoside-5-O-β-d-glucopyranoside, Cyanidin 3-O-(6-O-E-p-coumaroyl-2-O-β-d-xylopyranosyl)-β-d-glucopyranoside, and Cyanidin 3-O-(6-O-Z-p-coumaroyl-2-O-β-d-xylopyranosyl)-β-d-glucopyranoside. Keywords: Sambucus nigra; S. canadensis; Caprifoliaceae; elderberry; color stability; acylated anthocyanin
Osamu Inami - One of the best experts on this subject based on the ideXlab platform.
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stability of anthocyanins of sambucus canadensis and sambucus nigra
Journal of Agricultural and Food Chemistry, 1996Co-Authors: Osamu Inami, Itaru Tamura, Hiroe Kikuzaki, Nobuji NakataniAbstract:Elderberry anthocyanins (Sambucus nigra) including acylated derivatives (Sambucus canadensis) were studied for use as beverage pigments. Cyanidin 3-O-(6-O-E-p-coumaroyl-2-O-β-d-xylopyranosyl)-β-d-glucopyranoside-5-O-β-d-glucopyranoside from S. canadensis was more stable than Cyanidin 3-sambubioside from S. nigra. Acylation improved both heat and light stability, whereas glycosidation only stabilized anthocyanins in the presence of light. Cyanidin 3-(E)-p-coumaroyl-sambubioside-5-glucoside changed to three new anthocyanins under light irradiation. These three anthocyanins were isolated, and their structures were identified to be Cyanidin 3-O-(6-O-Z-p-coumaroyl-2-O-β-d-xylopyranosyl)-β-d-glucopyranoside-5-O-β-d-glucopyranoside, Cyanidin 3-O-(6-O-E-p-coumaroyl-2-O-β-d-xylopyranosyl)-β-d-glucopyranoside, and Cyanidin 3-O-(6-O-Z-p-coumaroyl-2-O-β-d-xylopyranosyl)-β-d-glucopyranoside. Keywords: Sambucus nigra; S. canadensis; Caprifoliaceae; elderberry; color stability; acylated anthocyanin
Steven F Bolling - One of the best experts on this subject based on the ideXlab platform.
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chemical profile and antioxidant capacities of tart cherry products
Food Chemistry, 2009Co-Authors: Ara Kirakosyan, E M Seymour, Daniel Urcuyo E Llanes, Peter B Kaufman, Steven F BollingAbstract:The levels of anthocyanins and other flavonoids, as well as melatonin, in various tart cherry products (frozen and dried cherries, powders from individually quick frozen (IQF) cherry and juice concentrate) from two tart cherry cultivars, ‘Montmorency’ and ‘Balaton’ were analysed comparatively by HPLC and electrospray mass spectrometry (EMS). Our results show that the major anthocyanin compound in these two tart cherry cultivars is Cyanidin 3-glucosylrutinoside, followed by Cyanidin 3-rutinoside, cyanydin sophoroside, and peonidin 3-glucoside. Studies on antioxidant activities (total antioxidant status assay) of crude extracts of ten tart cherry products show that these products preserve their antioxidant capacities after processing and storage. We have also compared the antioxidant activities of several single constituents that are present in tart cherry. When TEAC (trolox equivalent antioxidant capacity) values were evaluated conceptually against the cherry phytochemical profile, Cyanidin and its derivatives were found to be the significant contributors to the antioxidant systems of tart cherries. It was shown that standard compounds with common aglycon moieties show similar antioxidant activities.
Sirintorn Yibchokanun - One of the best experts on this subject based on the ideXlab platform.
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Cyanidin 3 rutinoside alleviates postprandial hyperglycemia and its synergism with acarbose by inhibition of intestinal α glucosidase
Journal of Clinical Biochemistry and Nutrition, 2011Co-Authors: Sirichai Adisakwattana, Sirintorn Yibchokanun, Piyawan Charoenlertkul, Natthakarn WongsasiripatAbstract:The inhibitory activity on intestinal α-glucosidase by Cyanidin-3-rutinoside was examined in vitro and in vivo. The IC50 values of Cyanidin-3-rutinoside against intestinal maltase, and sucrase were 2,323 ± 14.8 and 250.2 ± 8.1 µM, respectively. The kinetic analysis revealed that intestinal sucrase was inhibited by Cyanidin-3-rutinoside in a mixed-type manner. The synergistic inhibition also found in combination of Cyanidin-3-rutinoside with acarbose against intestinal maltase and sucrase. The oral administration of Cyanidin-3-rutinoside (100 and 300 mg/kg) plus maltose or sucrose to normal rats, postprandial plasma glucose was markedly suppressed at 30–90 min after loading. Furthermore, the normal rats treated with acarbose and Cyanidin-3-rutinoside (30 mg/kg) showed greater reduction of postprandial plasma glucose than the group treated with acarbose alone. These results suggest that Cyanidin-3-rutinoside retards absorption of carbohydrates by inhibition of α-glucosidase which may be useful as a potential inhibitor for prevention and treatment of diabetes mellitus.
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in vitro inhibitory effects of cyandin 3 rutinoside on pancreatic α amylase and its combined effect with acarbose
Molecules, 2011Co-Authors: Sarinya Akkarachiyasit, Sirintorn Yibchokanun, Sumrit Wacharasindhu, Sirichai AdisakwattanaAbstract:The inhibitory activity on pancreatic α-amylase by Cyanidin-3-rutinoside was examined in vitro. The IC50 value of Cyanidin-3-rutinoside against pancreatic α-amylase was 24.4 ± 0.1 μM. The kinetic analysis revealed that pancreatic α-amylase was inhibited by Cyanidin-3-rutinoside in a non-competitive manner. The additive inhibition of a combination of Cyanidin-3-rutinoside with acarbose against pancreatic α-amylase was also found. These results provide the first evidence for the effect of Cyanidin-3-rutinoside in a retarded absorption of carbohydrates by inhibition of pancreatic α-amylase which may be useful as a potential inhibitor for prevention and treatment of diabetes mellitus.
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inhibitory activities of Cyanidin and its glycosides and synergistic effect with acarbose against intestinal α glucosidase and pancreatic α amylase
International Journal of Molecular Sciences, 2010Co-Authors: Sarinya Akkarachiyasit, Piyawan Charoenlertkul, Sirintorn Yibchokanun, Sirichai AdisakwattanaAbstract:Cyanidin and its glycosides are naturally dietary pigments which have been indicated as promising candidates to have potential benefits to humans, especially in the prevention and treatment of diabetes mellitus. We investigated the structure activity relationships of Cyanidin and its glycosides to inhibit intestinal a-glucosidases and pancreatic a-amylase in vitro. The results found that Cyanidin and its glycosides are more specific inhibitors of intestinal sucrase than intestinal maltase. Cyanidin-3-galactoside and Cyanidin-3-glucoside were the most potent inhibitors against intestinal sucrase and pancreatic a-amylase with IC50 values of 0.50 ± 0.05 and 0.30 ± 0.01 mM, respectively. Our findings indicate that the structural difference between glucose and galactose at the 3-O-position of Cyanidin was an important factor for modulating the inhibition of intestinal sucrase and pancreatic a-amylase. The combination of cyandin-3-glucoside, Cyanidin-3-galactoside or Cyanidin-3,5-diglucosides with a low concentration of acarbose showed synergistic inhibition on intestinal maltase and sucrase. The synergistic inhibition was also found for a combination of Cyanidin or Cyanidin-3-glucoside with a low concentration of acarbose. The findings could provide a new insight into a use for the naturally occurring intestinal a-glucosidase and pancreatic a-amylase inhibitors for the prevention and treatment of diabetes and its complications.
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alpha glucosidase inhibitory activity of Cyanidin 3 galactoside and synergistic effect with acarbose
Journal of Enzyme Inhibition and Medicinal Chemistry, 2009Co-Authors: Sirichai Adisakwattana, Piyawan Charoenlertkul, Sirintorn YibchokanunAbstract:Cyanidin-3-galactoside, a natural anthocyanin, was investigated for its alpha-glucosidase inhibitory activity. The IC(50) value of Cyanidin-3-galactoside was 0.50 +/- 0.05 mM against intestinal sucrase. A low dose of Cyanidin-3-galactoside showed a synergistic inhibition on intestinal alpha-glucosidase (maltase and sucrase) when combined with acarbose. A kinetic analysis showed that Cyanidin-3-galactoside gave a mixed type inhibition against intestinal sucrase. The results indicated that Cyanidin-3-galactoside was an alpha-glucosidase inhibitor and could be used in combination with acarbose for treatment of diabetes.
