The Experts below are selected from a list of 39 Experts worldwide ranked by ideXlab platform
Ru Sun - One of the best experts on this subject based on the ideXlab platform.
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a mitochondria lysosome targeting fluorescence probe based on azonia Cyanine Dye and its application in nitroreductase detection
Sensors and Actuators B-chemical, 2020Co-Authors: Xinlong Sha, Xiuzhi Yang, Xuerui Wei, Ru SunAbstract:Abstract In this article, a benzo[e]indol aromatic azonia skeleton Cyanine Dye (3) with excellent performance was developed successfully through simple modification of an original aromatic azonia skeleton Cyanine Dye. Dye 3 had mitochondria/lysosome-targeting ability and its maximum emission wavelength extended to the near infrared region (655 nm). Inspired by this, probe 1a was designed, which connecting 2-bromomethyl-5-nitrofuran to the Cyanine Dye 3, and was the first mitochondria and lysosome dual-targeting probe for nitroreductase (NTR) detection. In addition to exhibiting good selectivity and fast response (180 s), probe 1a also exhibited high sensitivity to NTR (the detection limit was 3.2 ng/mL). The cell imaging experiments showed probe 1a could work as a dual mitochondria/lysosome-targeting probe to detect NTR in HeLa cells.
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A mitochondria/lysosome-targeting fluorescence probe based on azonia-Cyanine Dye and its application in nitroreductase detection
Sensors and Actuators B: Chemical, 2020Co-Authors: Xinlong Sha, Xiuzhi Yang, Xuerui Wei, Ru SunAbstract:Abstract In this article, a benzo[e]indol aromatic azonia skeleton Cyanine Dye (3) with excellent performance was developed successfully through simple modification of an original aromatic azonia skeleton Cyanine Dye. Dye 3 had mitochondria/lysosome-targeting ability and its maximum emission wavelength extended to the near infrared region (655 nm). Inspired by this, probe 1a was designed, which connecting 2-bromomethyl-5-nitrofuran to the Cyanine Dye 3, and was the first mitochondria and lysosome dual-targeting probe for nitroreductase (NTR) detection. In addition to exhibiting good selectivity and fast response (180 s), probe 1a also exhibited high sensitivity to NTR (the detection limit was 3.2 ng/mL). The cell imaging experiments showed probe 1a could work as a dual mitochondria/lysosome-targeting probe to detect NTR in HeLa cells.
Xinlong Sha - One of the best experts on this subject based on the ideXlab platform.
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a mitochondria lysosome targeting fluorescence probe based on azonia Cyanine Dye and its application in nitroreductase detection
Sensors and Actuators B-chemical, 2020Co-Authors: Xinlong Sha, Xiuzhi Yang, Xuerui Wei, Ru SunAbstract:Abstract In this article, a benzo[e]indol aromatic azonia skeleton Cyanine Dye (3) with excellent performance was developed successfully through simple modification of an original aromatic azonia skeleton Cyanine Dye. Dye 3 had mitochondria/lysosome-targeting ability and its maximum emission wavelength extended to the near infrared region (655 nm). Inspired by this, probe 1a was designed, which connecting 2-bromomethyl-5-nitrofuran to the Cyanine Dye 3, and was the first mitochondria and lysosome dual-targeting probe for nitroreductase (NTR) detection. In addition to exhibiting good selectivity and fast response (180 s), probe 1a also exhibited high sensitivity to NTR (the detection limit was 3.2 ng/mL). The cell imaging experiments showed probe 1a could work as a dual mitochondria/lysosome-targeting probe to detect NTR in HeLa cells.
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A mitochondria/lysosome-targeting fluorescence probe based on azonia-Cyanine Dye and its application in nitroreductase detection
Sensors and Actuators B: Chemical, 2020Co-Authors: Xinlong Sha, Xiuzhi Yang, Xuerui Wei, Ru SunAbstract:Abstract In this article, a benzo[e]indol aromatic azonia skeleton Cyanine Dye (3) with excellent performance was developed successfully through simple modification of an original aromatic azonia skeleton Cyanine Dye. Dye 3 had mitochondria/lysosome-targeting ability and its maximum emission wavelength extended to the near infrared region (655 nm). Inspired by this, probe 1a was designed, which connecting 2-bromomethyl-5-nitrofuran to the Cyanine Dye 3, and was the first mitochondria and lysosome dual-targeting probe for nitroreductase (NTR) detection. In addition to exhibiting good selectivity and fast response (180 s), probe 1a also exhibited high sensitivity to NTR (the detection limit was 3.2 ng/mL). The cell imaging experiments showed probe 1a could work as a dual mitochondria/lysosome-targeting probe to detect NTR in HeLa cells.
Xuerui Wei - One of the best experts on this subject based on the ideXlab platform.
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a mitochondria lysosome targeting fluorescence probe based on azonia Cyanine Dye and its application in nitroreductase detection
Sensors and Actuators B-chemical, 2020Co-Authors: Xinlong Sha, Xiuzhi Yang, Xuerui Wei, Ru SunAbstract:Abstract In this article, a benzo[e]indol aromatic azonia skeleton Cyanine Dye (3) with excellent performance was developed successfully through simple modification of an original aromatic azonia skeleton Cyanine Dye. Dye 3 had mitochondria/lysosome-targeting ability and its maximum emission wavelength extended to the near infrared region (655 nm). Inspired by this, probe 1a was designed, which connecting 2-bromomethyl-5-nitrofuran to the Cyanine Dye 3, and was the first mitochondria and lysosome dual-targeting probe for nitroreductase (NTR) detection. In addition to exhibiting good selectivity and fast response (180 s), probe 1a also exhibited high sensitivity to NTR (the detection limit was 3.2 ng/mL). The cell imaging experiments showed probe 1a could work as a dual mitochondria/lysosome-targeting probe to detect NTR in HeLa cells.
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A mitochondria/lysosome-targeting fluorescence probe based on azonia-Cyanine Dye and its application in nitroreductase detection
Sensors and Actuators B: Chemical, 2020Co-Authors: Xinlong Sha, Xiuzhi Yang, Xuerui Wei, Ru SunAbstract:Abstract In this article, a benzo[e]indol aromatic azonia skeleton Cyanine Dye (3) with excellent performance was developed successfully through simple modification of an original aromatic azonia skeleton Cyanine Dye. Dye 3 had mitochondria/lysosome-targeting ability and its maximum emission wavelength extended to the near infrared region (655 nm). Inspired by this, probe 1a was designed, which connecting 2-bromomethyl-5-nitrofuran to the Cyanine Dye 3, and was the first mitochondria and lysosome dual-targeting probe for nitroreductase (NTR) detection. In addition to exhibiting good selectivity and fast response (180 s), probe 1a also exhibited high sensitivity to NTR (the detection limit was 3.2 ng/mL). The cell imaging experiments showed probe 1a could work as a dual mitochondria/lysosome-targeting probe to detect NTR in HeLa cells.
Xiuzhi Yang - One of the best experts on this subject based on the ideXlab platform.
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a mitochondria lysosome targeting fluorescence probe based on azonia Cyanine Dye and its application in nitroreductase detection
Sensors and Actuators B-chemical, 2020Co-Authors: Xinlong Sha, Xiuzhi Yang, Xuerui Wei, Ru SunAbstract:Abstract In this article, a benzo[e]indol aromatic azonia skeleton Cyanine Dye (3) with excellent performance was developed successfully through simple modification of an original aromatic azonia skeleton Cyanine Dye. Dye 3 had mitochondria/lysosome-targeting ability and its maximum emission wavelength extended to the near infrared region (655 nm). Inspired by this, probe 1a was designed, which connecting 2-bromomethyl-5-nitrofuran to the Cyanine Dye 3, and was the first mitochondria and lysosome dual-targeting probe for nitroreductase (NTR) detection. In addition to exhibiting good selectivity and fast response (180 s), probe 1a also exhibited high sensitivity to NTR (the detection limit was 3.2 ng/mL). The cell imaging experiments showed probe 1a could work as a dual mitochondria/lysosome-targeting probe to detect NTR in HeLa cells.
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A mitochondria/lysosome-targeting fluorescence probe based on azonia-Cyanine Dye and its application in nitroreductase detection
Sensors and Actuators B: Chemical, 2020Co-Authors: Xinlong Sha, Xiuzhi Yang, Xuerui Wei, Ru SunAbstract:Abstract In this article, a benzo[e]indol aromatic azonia skeleton Cyanine Dye (3) with excellent performance was developed successfully through simple modification of an original aromatic azonia skeleton Cyanine Dye. Dye 3 had mitochondria/lysosome-targeting ability and its maximum emission wavelength extended to the near infrared region (655 nm). Inspired by this, probe 1a was designed, which connecting 2-bromomethyl-5-nitrofuran to the Cyanine Dye 3, and was the first mitochondria and lysosome dual-targeting probe for nitroreductase (NTR) detection. In addition to exhibiting good selectivity and fast response (180 s), probe 1a also exhibited high sensitivity to NTR (the detection limit was 3.2 ng/mL). The cell imaging experiments showed probe 1a could work as a dual mitochondria/lysosome-targeting probe to detect NTR in HeLa cells.
Bin Zhou - One of the best experts on this subject based on the ideXlab platform.
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Two novel protein chips for the detection of antibodies against porcine parvovirus
BMC veterinary research, 2020Co-Authors: Jinxiu Hou, Xiongnan Chen, Xiaobo Huang, Bin ZhouAbstract:PPV is one of the most important pathogens causing porcine reproductive disorder. It has been shown in clinical cases to be a commonly mixed infection with other important swine diseases which can aggravate the severity of the disease and bring serious economic losses to the pig industry. Serological methods, such as hemagglutination inhibition assays (HAI), serum neutralization (SN), and the modified direct complement-fixation (MDCF) test were utilized earlier, whereas the enzyme-linked immunosorbent assay (ELISA) is the most frequently applied assay to detect PPV-specific antibodies. We establish the visible protein chip and the Cyanine Dye 3 (Cy3)-labeled protein chip to detect the clinical serum from pigs. In this study, the recombinant protein VP2 of PPV was expressed in E.coli, purified with nickel magnetic beads, and then printed onto epoxy-coated glass slides for preparation of the protein chip. After a series of experiments, the conditions of antigen protein concentration, incubation time of primary antibody or secondary antibody, and optimal serum dilution fold were optimized, resulting in a successful visible protein chip and Cy3-labeled protein chip. The results showed that the positive serum, diluted up to 6000-fold, can be detected by the visible protein chip, and the positive serum, diluted up to 12,800-fold, can be detected by the Cy3-labeled protein chip, suggesting the high sensitivity of these protein chips. Moreover, the positive detection ratio, sensitivity, and specificity of these two kinds of protein chips were higher than those of commercial ELISA antibody detection kits. Overall, these two protein chips can be used to rapidly diagnose clinical samples with high throughput.
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Two novel protein chips for the detection of antibodies against porcine parvovirus
2020Co-Authors: Jinxiu Hou, Xiongnan Chen, Xiaobo Huang, Bin ZhouAbstract:Abstract Background : PPV is one of the most important pathogens causing porcine reproductive disorder. It has been shown in clinical cases to be a commonly mixed infection with other important swine diseases which can aggravate the severity of the disease and bring serious economic losses to the pig industry. Serological methods, such as hemagglutination inhibition assays (HAI), serum neutralization (SN), and the modified direct complement-fixation (MDCF) test were utilized earlier, whereas the enzyme-linked immunosorbent assay (ELISA) is the most frequently applied assay to detect PPV-specific antibodies Results: We establish the visible protein chip and the Cyanine Dye 3 (Cy3)-labeled protein chip to detect the clinical serum from pigs. In this study, the recombinant protein VP2 of PPV was expressed in E.coli , purified with nickel magnetic beads, and then printed onto epoxy-coated glass slides for preparation of the protein chip. After a series of experiments, the conditions of antigen protein concentration, incubation time of primary antibody or secondary antibody, and optimal serum dilution fold were optimized, resulting in a successful visible protein chip and Cy3-labeled protein chip. The results showed that the positive serum, diluted up to 6000-fold, can be detected by the visible protein chip, and the positive serum, diluted up to 12,800-fold, can be detected by the Cy3-labeled protein chip, suggesting the high sensitivity of these protein chips. Moreover, the positive detection ratio, sensitivity, and specificity of these two kinds of protein chips were higher than those of commercial ELISA antibody detection kits. Conclusion: Overall, these two protein chips can be used to rapidly diagnose clinical samples with high throughput. Key words: Protein chip; Porcine parvovirus (PPV); Antibody detection; Clinical serum
