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John M. Hutson - One of the best experts on this subject based on the ideXlab platform.
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Calcitonin gene-related peptide stimulates motility of the gubernaculum via Cyclic Adenosine Monophosphate.
The Journal of urology, 1993Co-Authors: Yoshitaka Momose, John M. HutsonAbstract:Calcitonin gene-related peptide (CGRP), N-truncated CGRP fragments CGRP 8-37 and [Tyr0]-CGRP 28-37, and dibutyryl Cyclic Adenosine Monophosphate (DBcAMP) were studied for their effects on the neonatal male mouse gubernaculum in organ culture. Rhythmic contractions were shown in 18% of control gubernacula, which were enhanced with CGRP, inhibited by CGRP 8-37 and not affected by [Tyr0]-CGRP 28-37. A total of 60 gubernacula was exposed to increasing concentrations of DBcAMP and the percentage of gubernacula showing rhythmic contractions increased from 18 to 60%. These studies demonstrate that the neonatal mouse gubernaculum exhibits endogenous contractility that can be enhanced with CGRP or DBcAMP. These results suggest that Cyclic Adenosine Monophosphate may act as the intracellular second messenger for receptor bound CGRP in the gubernaculum. This finding is consistent with the hypothesis that CGRP from the genitofemoral nerve provides directional, chemotactic guidance for inguinoscrotal gubernacular migration during testicular descent.
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calcitonin gene related peptide stimulates motility of the gubernaculum via Cyclic Adenosine Monophosphate
The Journal of Urology, 1993Co-Authors: Yoshitaka Momose, John M. HutsonAbstract:AbstractCalcitonin gene-related peptide (CGRP), N-truncated CGRP fragments CGRP 8-37 and [Tyr0]-CGRP 28-37, and dibutyryl Cyclic Adenosine Monophosphate (DBcAMP) were studied for their effects on the neonatal male mouse gubernaculum in organ culture. Rhythmic contractions were shown in 18% of control gubernacula, which were enhanced with CGRP, inhibited by CGRP 8-37 and not affected by [Tyr0]-CGRP 28-37. A total of 60 gubernacula was exposed to increasing concentrations of DBcAMP and the percentage of gubernacula showing rhythmic contractions increased from 18 to 60%. These studies demonstrate that the neonatal mouse gubernaculum exhibits endogenous contractility that can be enhanced with CGRP or DBcAMP. These results suggest that Cyclic Adenosine Monophosphate may act as the intracellular second messenger for receptor bound CGRP in the gubernaculum. This finding is consistent with the hypothesis that CGRP from the genitofemoral nerve provides directional, chemotactic guidance for inguinoscrotal gubernacu...
Yoshifumi Naka - One of the best experts on this subject based on the ideXlab platform.
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Influence of ischemic injury on vein graft remodeling: Role of Cyclic Adenosine Monophosphate second messenger pathway in enhanced vein graft preservation
The Journal of thoracic and cardiovascular surgery, 2005Co-Authors: Taichi Sakaguchi, Tomohiro Asai, Dmitri Belov, Morihito Okada, David J. Pinsky, Ann Marie Schmidt, Yoshifumi NakaAbstract:Abstract Objective Endothelial injury during the harvest of saphenous vein grafts might play an important role in the development of vein graft disease after coronary artery bypass grafting. Using a murine autologous arterialized vein patch model, we tested whether the initial ischemic insult of vein grafts was linked to the later development of graft neointimal hyperplasia and whether the restoration of the Cyclic Adenosine Monophosphate second messenger pathway would attenuate the development of neointimal hyperplasia. Methods A segment of the external jugular vein of a mouse was grafted onto its abdominal aorta. Three weeks after the operation, the degree of neointimal hyperplasia of the implanted graft was compared among (1) grafts without preservation, (2) grafts with 2 hours of preservation (25°C) in heparinized saline, and (3) grafts with 2 hours of preservation in heparinized saline in the presence of a Cyclic Adenosine Monophosphate analog. In addition, Cyclic Adenosine Monophosphate contents of vein grafts and leukocyte adherence to the graft endothelium were assessed. Results Cyclic Adenosine Monophosphate contents were significantly decreased after 2 hours of preservation (212 ± 8 vs 156 ± 5 pmol/L, P Conclusions Ischemic insult during vein graft harvest and preservation is a key factor in the development of vein graft neointimal hyperplasia at least in part caused by the depletion of Cyclic Adenosine Monophosphate. We conclude that stimulation of the Cyclic Adenosine Monophosphate second messenger pathway might be a potential strategy for the prevention of vein graft disease.
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Superior protection in orthotopic rat lung transplantation with Cyclic Adenosine Monophosphate and nitroglycerin–containing preservation solution☆☆☆★
The Journal of thoracic and cardiovascular surgery, 1999Co-Authors: Koichi Kayano, Yoshifumi Naka, Koichi Toda, Kenji Okada, David J. PinskyAbstract:Abstract Background: Primary lung graft failure is common, and current lung preservation strategies are suboptimal. Because the decline in lung levels of Cyclic Adenosine Monophosphate and Cyclic guanosine Monophosphate during preservation could enhance adhesiveness of endothelial cells for leukocytes as well as increase vascular permeability and vasoconstriction, we hypothesized that buttressing these levels by means of a preservation solution would significantly improve lung preservation. Methods: An orthotopic rat left lung transplantation model was used. Lungs were harvested from male Lewis rats and preserved for 6 hours at 4°C with (1) Euro-Collins solution (n = 8); (2) University of Wisconsin solution (n = 8); (3) low-potassium dextran glucose solution (n = 8); (4) Columbia University solution (n = 8), which contains a Cyclic Adenosine Monophosphate analog (dibutyryl Cyclic Adenosine Monophosphate) and a nitric oxide donor (nitroglycerin) to buttress Cyclic guanosine Monophosphate levels; or (5) Columbia University solution without Cyclic Adenosine Monophosphate or nitroglycerin (n = 8). PaO2, pulmonary vascular resistance, and recipient survival were evaluated 30 minutes after left lung transplantation and removal of the nontransplanted right lung from the pulmonary circulation. Results: Among all groups studied, grafts stored with Columbia University solution demonstrated the highest Pa O2 (355 ± 25 mm Hg for Columbia University solution versus 95 ± 22 mm Hg for Euro-Collins solution, P P P P –1 • min –1 for Columbia University solution versus 12 ± 4 mm Hg • mL –1 • min –1 for Euro-Collins solution, P –1 • min –1 for University of Wisconsin solution, 14 ± 6 mm Hg • mL –1 • min –1 for low-potassium dextran glucose solution, P –1 • min –1 for Columbia University solution without Cyclic Adenosine Monophosphate and nitroglycerin). These functional and hemodynamic improvements provided by Columbia University solution were accompanied by decreased graft leukostasis and decreased recipient tumor necrosis factor α and interleukin 1α levels compared with the other groups. In toto, these improvements translated into superior survival among recipients of Columbia University solution–preserved grafts (100% for Columbia University solution, 37% for Euro-Collins solution, P P P P Conclusion: Nitroglycerin and Cyclic Adenosine Monophosphate confer beneficial vascular effects that make Columbia University solution a superior lung preservation solution in a stringent rat lung transplantation model. (J Thorac Cardiovasc Surg 1999;118:135-44)
Yoshitaka Momose - One of the best experts on this subject based on the ideXlab platform.
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Calcitonin gene-related peptide stimulates motility of the gubernaculum via Cyclic Adenosine Monophosphate.
The Journal of urology, 1993Co-Authors: Yoshitaka Momose, John M. HutsonAbstract:Calcitonin gene-related peptide (CGRP), N-truncated CGRP fragments CGRP 8-37 and [Tyr0]-CGRP 28-37, and dibutyryl Cyclic Adenosine Monophosphate (DBcAMP) were studied for their effects on the neonatal male mouse gubernaculum in organ culture. Rhythmic contractions were shown in 18% of control gubernacula, which were enhanced with CGRP, inhibited by CGRP 8-37 and not affected by [Tyr0]-CGRP 28-37. A total of 60 gubernacula was exposed to increasing concentrations of DBcAMP and the percentage of gubernacula showing rhythmic contractions increased from 18 to 60%. These studies demonstrate that the neonatal mouse gubernaculum exhibits endogenous contractility that can be enhanced with CGRP or DBcAMP. These results suggest that Cyclic Adenosine Monophosphate may act as the intracellular second messenger for receptor bound CGRP in the gubernaculum. This finding is consistent with the hypothesis that CGRP from the genitofemoral nerve provides directional, chemotactic guidance for inguinoscrotal gubernacular migration during testicular descent.
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calcitonin gene related peptide stimulates motility of the gubernaculum via Cyclic Adenosine Monophosphate
The Journal of Urology, 1993Co-Authors: Yoshitaka Momose, John M. HutsonAbstract:AbstractCalcitonin gene-related peptide (CGRP), N-truncated CGRP fragments CGRP 8-37 and [Tyr0]-CGRP 28-37, and dibutyryl Cyclic Adenosine Monophosphate (DBcAMP) were studied for their effects on the neonatal male mouse gubernaculum in organ culture. Rhythmic contractions were shown in 18% of control gubernacula, which were enhanced with CGRP, inhibited by CGRP 8-37 and not affected by [Tyr0]-CGRP 28-37. A total of 60 gubernacula was exposed to increasing concentrations of DBcAMP and the percentage of gubernacula showing rhythmic contractions increased from 18 to 60%. These studies demonstrate that the neonatal mouse gubernaculum exhibits endogenous contractility that can be enhanced with CGRP or DBcAMP. These results suggest that Cyclic Adenosine Monophosphate may act as the intracellular second messenger for receptor bound CGRP in the gubernaculum. This finding is consistent with the hypothesis that CGRP from the genitofemoral nerve provides directional, chemotactic guidance for inguinoscrotal gubernacu...
David J. Pinsky - One of the best experts on this subject based on the ideXlab platform.
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Influence of ischemic injury on vein graft remodeling: Role of Cyclic Adenosine Monophosphate second messenger pathway in enhanced vein graft preservation
The Journal of thoracic and cardiovascular surgery, 2005Co-Authors: Taichi Sakaguchi, Tomohiro Asai, Dmitri Belov, Morihito Okada, David J. Pinsky, Ann Marie Schmidt, Yoshifumi NakaAbstract:Abstract Objective Endothelial injury during the harvest of saphenous vein grafts might play an important role in the development of vein graft disease after coronary artery bypass grafting. Using a murine autologous arterialized vein patch model, we tested whether the initial ischemic insult of vein grafts was linked to the later development of graft neointimal hyperplasia and whether the restoration of the Cyclic Adenosine Monophosphate second messenger pathway would attenuate the development of neointimal hyperplasia. Methods A segment of the external jugular vein of a mouse was grafted onto its abdominal aorta. Three weeks after the operation, the degree of neointimal hyperplasia of the implanted graft was compared among (1) grafts without preservation, (2) grafts with 2 hours of preservation (25°C) in heparinized saline, and (3) grafts with 2 hours of preservation in heparinized saline in the presence of a Cyclic Adenosine Monophosphate analog. In addition, Cyclic Adenosine Monophosphate contents of vein grafts and leukocyte adherence to the graft endothelium were assessed. Results Cyclic Adenosine Monophosphate contents were significantly decreased after 2 hours of preservation (212 ± 8 vs 156 ± 5 pmol/L, P Conclusions Ischemic insult during vein graft harvest and preservation is a key factor in the development of vein graft neointimal hyperplasia at least in part caused by the depletion of Cyclic Adenosine Monophosphate. We conclude that stimulation of the Cyclic Adenosine Monophosphate second messenger pathway might be a potential strategy for the prevention of vein graft disease.
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Superior protection in orthotopic rat lung transplantation with Cyclic Adenosine Monophosphate and nitroglycerin–containing preservation solution☆☆☆★
The Journal of thoracic and cardiovascular surgery, 1999Co-Authors: Koichi Kayano, Yoshifumi Naka, Koichi Toda, Kenji Okada, David J. PinskyAbstract:Abstract Background: Primary lung graft failure is common, and current lung preservation strategies are suboptimal. Because the decline in lung levels of Cyclic Adenosine Monophosphate and Cyclic guanosine Monophosphate during preservation could enhance adhesiveness of endothelial cells for leukocytes as well as increase vascular permeability and vasoconstriction, we hypothesized that buttressing these levels by means of a preservation solution would significantly improve lung preservation. Methods: An orthotopic rat left lung transplantation model was used. Lungs were harvested from male Lewis rats and preserved for 6 hours at 4°C with (1) Euro-Collins solution (n = 8); (2) University of Wisconsin solution (n = 8); (3) low-potassium dextran glucose solution (n = 8); (4) Columbia University solution (n = 8), which contains a Cyclic Adenosine Monophosphate analog (dibutyryl Cyclic Adenosine Monophosphate) and a nitric oxide donor (nitroglycerin) to buttress Cyclic guanosine Monophosphate levels; or (5) Columbia University solution without Cyclic Adenosine Monophosphate or nitroglycerin (n = 8). PaO2, pulmonary vascular resistance, and recipient survival were evaluated 30 minutes after left lung transplantation and removal of the nontransplanted right lung from the pulmonary circulation. Results: Among all groups studied, grafts stored with Columbia University solution demonstrated the highest Pa O2 (355 ± 25 mm Hg for Columbia University solution versus 95 ± 22 mm Hg for Euro-Collins solution, P P P P –1 • min –1 for Columbia University solution versus 12 ± 4 mm Hg • mL –1 • min –1 for Euro-Collins solution, P –1 • min –1 for University of Wisconsin solution, 14 ± 6 mm Hg • mL –1 • min –1 for low-potassium dextran glucose solution, P –1 • min –1 for Columbia University solution without Cyclic Adenosine Monophosphate and nitroglycerin). These functional and hemodynamic improvements provided by Columbia University solution were accompanied by decreased graft leukostasis and decreased recipient tumor necrosis factor α and interleukin 1α levels compared with the other groups. In toto, these improvements translated into superior survival among recipients of Columbia University solution–preserved grafts (100% for Columbia University solution, 37% for Euro-Collins solution, P P P P Conclusion: Nitroglycerin and Cyclic Adenosine Monophosphate confer beneficial vascular effects that make Columbia University solution a superior lung preservation solution in a stringent rat lung transplantation model. (J Thorac Cardiovasc Surg 1999;118:135-44)
Stefania Mitola - One of the best experts on this subject based on the ideXlab platform.
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Cyclic Adenosine Monophosphate response element binding protein mediates the proangiogenic or proinflammatory activity of gremlin
Arteriosclerosis Thrombosis and Vascular Biology, 2014Co-Authors: Michela Corsini, Emanuela Moroni, Cosetta Ravelli, Germán Andrés, Elisabetta Grillo, Imran H A Ali, Derek P. Brazil, Marco Presta, Stefania MitolaAbstract:Objective—Angiogenesis and inflammation are closely related processes. Gremlin is a novel noncanonical vascular endothelial growth factor receptor-2 (VEGFR2) ligand that induces a proangiogenic response in endothelial cells (ECs). Here, we investigated the role of the Cyclic Adenosine Monophosphate-response element (CRE)–binding protein (CREB) in mediating the proinflammatory and proangiogenic responses of ECs to gremlin. Approach and Results—Gremlin induces a proinflammatory response in ECs, leading to reactive oxygen species and Cyclic Adenosine Monophosphate production and the upregulation of proinflammatory molecules involved in leukocyte extravasation, including chemokine (C-C motif) ligand-2 (Ccl2) and Ccl7, chemokine (C-X-C motif) ligand-1 (Cxcl1), vascular cell adhesion molecule-1 (VCAM-1), and intercellular adhesion molecule-1 (ICAM-1). Accordingly, gremlin induces the VEGFR2-dependent phosphorylation, nuclear translocation, and transactivating activity of CREB in ECs. CREB activation mediates th...
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Cyclic Adenosine Monophosphate-Response Element–Binding Protein Mediates the Proangiogenic or Proinflammatory Activity of Gremlin
Arteriosclerosis thrombosis and vascular biology, 2013Co-Authors: Michela Corsini, Emanuela Moroni, Cosetta Ravelli, Germán Andrés, Elisabetta Grillo, Imran H A Ali, Derek P. Brazil, Marco Presta, Stefania MitolaAbstract:Objective—Angiogenesis and inflammation are closely related processes. Gremlin is a novel noncanonical vascular endothelial growth factor receptor-2 (VEGFR2) ligand that induces a proangiogenic response in endothelial cells (ECs). Here, we investigated the role of the Cyclic Adenosine Monophosphate-response element (CRE)–binding protein (CREB) in mediating the proinflammatory and proangiogenic responses of ECs to gremlin. Approach and Results—Gremlin induces a proinflammatory response in ECs, leading to reactive oxygen species and Cyclic Adenosine Monophosphate production and the upregulation of proinflammatory molecules involved in leukocyte extravasation, including chemokine (C-C motif) ligand-2 (Ccl2) and Ccl7, chemokine (C-X-C motif) ligand-1 (Cxcl1), vascular cell adhesion molecule-1 (VCAM-1), and intercellular adhesion molecule-1 (ICAM-1). Accordingly, gremlin induces the VEGFR2-dependent phosphorylation, nuclear translocation, and transactivating activity of CREB in ECs. CREB activation mediates th...
