The Experts below are selected from a list of 34659 Experts worldwide ranked by ideXlab platform
Liu Yun - One of the best experts on this subject based on the ideXlab platform.
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induction of gynogenesis in red crucian carp using spermatozoa of blunt snout bream
Progress in Natural Science, 2007Co-Authors: Sun Yuandong, Tao Min, Liu Shaojun, Zhang Chun, Duan Wei, Shen Jiamin, Wang Jing, Zeng Chen, Liu YunAbstract:Abstract Gynogenetic diploid was induced in red crucian carp (RCC) (Carassius auratus Red Variety) eggs using UV-irradiated spermatozoa from blunt snout bream (B) (Megalabrama amblycephala) or from mirror carp (C) (Cyprinus carpio. L). Spermatozoa were genetically inactivated by an appropriate UV dosage, and then the maternal DNA was duplicated with cold shock at 0–4°C. When using the spermatozoa of B, the fertilization rate, hatching rate and survival at first feeding were 52.6 ± 3.0 %, 23.6 ± 4.1 % and 15.7 ± 3.4 % respectively, and the survival at first feeding was significantly higher than that (11.3 ± 2.2%) when using the spermatozoa of C(Cypripnus carpio. L). According to the morphological characteristics, the chromosome number and the degree of gonadal development, gynogenetic RCC could be distinguished from the control hybrids of RCC ♀ × B♂. The individuals with red body color, 100 chromosomes and normal gonadal development were syccessful gyogenetic RCC, while the individuals with 124 or 148 chro...
Li-qun Liang - One of the best experts on this subject based on the ideXlab platform.
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Development of 10 tri- and tetranucleotide microsatellite loci for population studies in the common carp (Cyprinus carpio L.).
Molecular ecology resources, 2008Co-Authors: Ning Hou, Xiaowen Sun, Li-qun LiangAbstract:Ten tri- and tetranucleotide microsatellite DNA markers were isolated and characterized from common carp (Cyprinus carpio L.) to estimate genetic potential. These markers were tested in the samples from two closely related carp populations (Cyprinus carpio var. xingguonensis and Cyprinus carpio var. wananensis). The number of the alleles ranged from three to nine, and observed and expected hererozygosities varied from 0.207 to 1.000 and from 0.499 to 0.900 in each population, respectively. No evidence for linkage disequilibrium was found, indicating that these markers will be useful for population studies.
Sun Yuandong - One of the best experts on this subject based on the ideXlab platform.
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induction of gynogenesis in red crucian carp using spermatozoa of blunt snout bream
Progress in Natural Science, 2007Co-Authors: Sun Yuandong, Tao Min, Liu Shaojun, Zhang Chun, Duan Wei, Shen Jiamin, Wang Jing, Zeng Chen, Liu YunAbstract:Abstract Gynogenetic diploid was induced in red crucian carp (RCC) (Carassius auratus Red Variety) eggs using UV-irradiated spermatozoa from blunt snout bream (B) (Megalabrama amblycephala) or from mirror carp (C) (Cyprinus carpio. L). Spermatozoa were genetically inactivated by an appropriate UV dosage, and then the maternal DNA was duplicated with cold shock at 0–4°C. When using the spermatozoa of B, the fertilization rate, hatching rate and survival at first feeding were 52.6 ± 3.0 %, 23.6 ± 4.1 % and 15.7 ± 3.4 % respectively, and the survival at first feeding was significantly higher than that (11.3 ± 2.2%) when using the spermatozoa of C(Cypripnus carpio. L). According to the morphological characteristics, the chromosome number and the degree of gonadal development, gynogenetic RCC could be distinguished from the control hybrids of RCC ♀ × B♂. The individuals with red body color, 100 chromosomes and normal gonadal development were syccessful gyogenetic RCC, while the individuals with 124 or 148 chro...
Ning Hou - One of the best experts on this subject based on the ideXlab platform.
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Development of 10 tri- and tetranucleotide microsatellite loci for population studies in the common carp (Cyprinus carpio L.).
Molecular ecology resources, 2008Co-Authors: Ning Hou, Xiaowen Sun, Li-qun LiangAbstract:Ten tri- and tetranucleotide microsatellite DNA markers were isolated and characterized from common carp (Cyprinus carpio L.) to estimate genetic potential. These markers were tested in the samples from two closely related carp populations (Cyprinus carpio var. xingguonensis and Cyprinus carpio var. wananensis). The number of the alleles ranged from three to nine, and observed and expected hererozygosities varied from 0.207 to 1.000 and from 0.499 to 0.900 in each population, respectively. No evidence for linkage disequilibrium was found, indicating that these markers will be useful for population studies.
Chitao Li - One of the best experts on this subject based on the ideXlab platform.
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a novel 79 bp insertion deletion polymorphism in 3 flanking region of igf i gene is associated with growth related traits in common carp Cyprinus carpio l
Aquaculture Research, 2013Co-Authors: Xuesong Hu, Chitao LiAbstract:Insulin-like growth factor-I (IGF-I) participates in the modulation of the cellular development, differentiation and proliferation in various vertebrate tissues. Therefore, variations in IGF-I gene are good candidates as growth regulators. In this study, a novel 79-bp insertion/deletion (I/D) polymorphism was identified in 3′-flanking region of IGF-I gene by polymerase chain reaction (PCR) and sequencing approaches in common carp Cyprinus carpio. To gain insight into the association of the 79-bp I/D polymorphism with growth-related traits, a total of 747 individuals from Heilongjiang carp Cyprinus cario haematopterus (YL) (n = 263), selective line of German mirror carp Cyprinus carpio L. mirror (JL) (n = 229), and cold-resistance line of Hebao red carp Cyprinus carpio var. wuyuanensis (HL) (n = 255) were genotyped. The DD genotype of the polymorphism was significantly associated with higher body length (135), body weight (325, 385, and 445 days), net weight (445 days) and lower overwintering body loss rate in JL. In addition, in pooled population (YL+JL+HL), fish with DD genotype also showed significantly higher body weight (445 days) and net weight (445 days) than those with II genotype. Taken together, these results suggest that the novel polymorphism in IGF-I gene could be considered as a potential genetic marker for selection of growth performance in common carp.
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A novel 79‐bp insertion/deletion polymorphism in 3′‐flanking region of IGF‐I gene is associated with growth‐related traits in common carp (Cyprinus carpio L.)
Aquaculture Research, 2012Co-Authors: Xuesong Hu, Chitao LiAbstract:Insulin-like growth factor-I (IGF-I) participates in the modulation of the cellular development, differentiation and proliferation in various vertebrate tissues. Therefore, variations in IGF-I gene are good candidates as growth regulators. In this study, a novel 79-bp insertion/deletion (I/D) polymorphism was identified in 3′-flanking region of IGF-I gene by polymerase chain reaction (PCR) and sequencing approaches in common carp Cyprinus carpio. To gain insight into the association of the 79-bp I/D polymorphism with growth-related traits, a total of 747 individuals from Heilongjiang carp Cyprinus cario haematopterus (YL) (n = 263), selective line of German mirror carp Cyprinus carpio L. mirror (JL) (n = 229), and cold-resistance line of Hebao red carp Cyprinus carpio var. wuyuanensis (HL) (n = 255) were genotyped. The DD genotype of the polymorphism was significantly associated with higher body length (135), body weight (325, 385, and 445 days), net weight (445 days) and lower overwintering body loss rate in JL. In addition, in pooled population (YL+JL+HL), fish with DD genotype also showed significantly higher body weight (445 days) and net weight (445 days) than those with II genotype. Taken together, these results suggest that the novel polymorphism in IGF-I gene could be considered as a potential genetic marker for selection of growth performance in common carp.
