The Experts below are selected from a list of 663645 Experts worldwide ranked by ideXlab platform
J. Paul Robinson - One of the best experts on this subject based on the ideXlab platform.
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Overview of Flow Cytometry and Microbiology: Overview of Flow Cytometry and Microbiology
Current protocols in immunology, 2018Co-Authors: J. Paul RobinsonAbstract:Although in recent years flow Cytometry has become commonplace in hematology and immunology laboratories, application of the technology to microbiology remains largely unrealized. This overview presents the historical background, discusses applications in various areas of the field, and speculates on the directions of future developments. The availability of high-quality methods should be a prime factor in convincing microbiologists that flow Cytometry may have certain advantages over traditional methods and that it does indeed have much to contribute to microbiology. © 2018 by John Wiley & Sons, Inc.
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Current Protocols in Cytometry - Overview of Flow Cytometry and Microbiology
Current protocols in cytometry, 2018Co-Authors: J. Paul RobinsonAbstract:Although in recent years flow Cytometry has become commonplace in hematology and immunology laboratories, application of the technology to microbiology remains largely unrealized. This overview presents the historical background, discusses applications in various areas of the field, and speculates on the directions of future developments. The availability of high-quality methods should be a prime factor in convincing microbiologists that flow Cytometry may have certain advantages over traditional methods and that it does indeed have much to contribute to microbiology. © 2018 by John Wiley & Sons, Inc.
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Flow Cytometry strikes gold
Science, 2015Co-Authors: J. Paul Robinson, Mario RoedererAbstract:This month marks the 50th anniversary of the birth of flow Cytometry. One would imagine that a technology invented so long ago would be radically different today—yet it has changed remarkably little in its fundamentals. It allows rapid and simultaneous analysis of multiple parameters of live cells in a heterogeneous mix as they flow in a stream through photonic detectors. The data are integrated to give a comprehensive view of the sample. Flow Cytometry has become a staple of biological research as well as clinical diagnostics, and its application has been crucial to innumerable advances in immunology and cell biology, and for understanding diseases such as AIDS and cancer. It has spawned the development of dozens of other key technologies and its application continues to expand, ranging from single-cell to cell population analysis.
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Current Protocols in Cytometry - Comparative overview of flow and image Cytometry.
Current Protocols in Cytometry, 2005Co-Authors: J. Paul RobinsonAbstract:This unit considers the issues of which tool to use—flow Cytometry or imaging—and under what conditions. In particular, it compares the advantages and disadvantages of flow and image Cytometry and provides examples illustrating the proper choice of each technology. The result is a better understanding of why the two technologies are complementary in many applications. It is clear that many scientists use the tools that are familiar to them, often in preference to the best tool. In cases where very advanced and rather expensive technologies are concerned, this is not surprising. However, there are clearly times when one form of Cytometry is definitely superior to another. What then constitute the criteria for a decision when both flow Cytometry and imaging are available? This unit addresses some of these concerns. Keywords: flow Cytometry; image Cytometry; fluorescence measurement
Ivan A. Vorobjev - One of the best experts on this subject based on the ideXlab platform.
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Imaging Flow Cytometry - Imaging flow Cytometry
Journal of Histochemistry and Cytochemistry, 2016Co-Authors: Natasha S Barteneva, Elizaveta Fasler-kan, Ivan A. VorobjevAbstract:Imaging flow Cytometry (IFC) platforms combine features of flow Cytometry and fluorescent microscopy with advances in data-processing algorithms. IFC allows multiparametric fluorescent and morphological analysis of thousands of cellular events and has the unique capability of identifying collected events by their real images. IFC allows the analysis of heterogeneous cell populations, where one of the cellular components has low expression (
Depei Wu - One of the best experts on this subject based on the ideXlab platform.
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transcription factor pax5 promotes b lymphomagenesis in a promoter independent manner
Zhongguo shi yan xue ye xue za zhi, 2017Co-Authors: Yanqing Zhang, Duonan Yu, Depei WuAbstract:OBJECTIVE: To determine whether B lymphocyte-specific transcription factor Pax5 regulates B-lympho-magenesis without direct binding to promoter. METHODS: Mouse B-lymphoma cell line myc3 and 38B9 were infected with GFP- tagged retrovirus that encodes wide type or various mutant pax5 genes. After viral infection for 48 hours, the percentage of GFP positive lymphoma cells was determined by flow cytomety. The percentage of GFP positive tumor cells was further monitored every 3 days in vitro or once the tumor was formed in vivo. Both cell cycle and apoptic cell number of GFP positive lymphoma cells were analyzed using flow Cytometry. RESULTS: Similar to the infection with wild type Pax5 retrovirus, infection with Pax5 mt 1-357 and Pax5 mt 304-358 that lacks of DNA binding motif can strongly increase the percentage of GFP+ B-lymphoma cells both in vitro and in vivo (P 0.05). Moreover, the analysis of flow Cytometry demonstrated that more B-lymphoma cells infected with wild type Pax5, Pax5 mt 1-357 and Pax5 mt 304-358 retroviruses entered S and G2/M phases in comparison with those infected with empty viral vector migR-GFP and Pax5 mt 1-143. Apoptotic rates among different groups were not significantly changed. CONCLUSION: Pax5 can promote B-lymphoma cell growth both in vitro and in vivo in a promoter-independent manner. This is mainly due to the accelerating of cell cycle rather than decreasing apoptosis. Our studies provide potential theory for restraing B-lymphomagenesis by targeting the specific Pax5 domains.
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transcription factor pax5 promotes b lymphomagenesis in a promoter independent manner
Zhongguo shi yan xue ye xue za zhi, 2017Co-Authors: Yanqing Zhang, Duonan Yu, Depei WuAbstract:OBJECTIVE: To determine whether B lymphocyte-specific transcription factor Pax5 regulates B-lympho-magenesis without direct binding to promoter. METHODS: Mouse B-lymphoma cell line myc3 and 38B9 were infected with GFP- tagged retrovirus that encodes wide type or various mutant pax5 genes. After viral infection for 48 hours, the percentage of GFP positive lymphoma cells was determined by flow cytomety. The percentage of GFP positive tumor cells was further monitored every 3 days in vitro or once the tumor was formed in vivo. Both cell cycle and apoptic cell number of GFP positive lymphoma cells were analyzed using flow Cytometry. RESULTS: Similar to the infection with wild type Pax5 retrovirus, infection with Pax5 mt 1-357 and Pax5 mt 304-358 that lacks of DNA binding motif can strongly increase the percentage of GFP+ B-lymphoma cells both in vitro and in vivo (P 0.05). Moreover, the analysis of flow Cytometry demonstrated that more B-lymphoma cells infected with wild type Pax5, Pax5 mt 1-357 and Pax5 mt 304-358 retroviruses entered S and G2/M phases in comparison with those infected with empty viral vector migR-GFP and Pax5 mt 1-143. Apoptotic rates among different groups were not significantly changed. CONCLUSION: Pax5 can promote B-lymphoma cell growth both in vitro and in vivo in a promoter-independent manner. This is mainly due to the accelerating of cell cycle rather than decreasing apoptosis. Our studies provide potential theory for restraing B-lymphomagenesis by targeting the specific Pax5 domains.
David W. Hedley - One of the best experts on this subject based on the ideXlab platform.
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Imaging Mass Cytometry
Cytometry. Part A : the journal of the International Society for Analytical Cytology, 2017Co-Authors: Qing Chang, Olga Ornatsky, Iram Siddiqui, Alexander Loboda, Vladimir Baranov, David W. HedleyAbstract:Imaging Mass Cytometry (IMC) is an expansion of mass Cytometry, but rather than analyzing single cells in suspension, it uses laser ablation to generate plumes of particles that are carried to the mass cytometer by a stream of inert gas. Images reconstructed from tissue sections scanned by IMC have a resolution comparable to light microscopy, with the high content of mass Cytometry enabled through the use of isotopically labeled probes and ICP-MS detection. Importantly, IMC can be performed on paraffin-embedded tissue sections, so can be applied to the retrospective analysis of patient cohorts whose outcome is known, and eventually to personalized medicine. Since the original description in 2014, IMC has evolved rapidly into a commercial instrument of unprecedented power for the analysis of histological sections. In this Review, we discuss the underlying principles of this new technology, and outline emerging applications of IMC in the analysis of normal and pathological tissues. © 2017 International Society for Advancement of Cytometry.
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DNA Cytometry Consensus Conference. DNA flow Cytometry and breast cancer.
Breast cancer research and treatment, 1993Co-Authors: David W. HedleyAbstract:Measurement of cellular DNA content by flow Cytometry is capable of detecting aneuploid stemlines, and also of giving an indication of tumor proliferation kinetics by approximating the percentage of cells in S-phase of the replicative cycle. Because it can be applied both to fresh frozen material submitted for steroid hormone receptor analysis and to fixed paraffin-embedded blocks, it is particularly well suited to the study of breast cancer. Despite being a relatively straightforward test which is now widely used in the risk assessment of patients with early breast cancer, in common with many other prognostic markers its precise clinical role remains uncertain. An extensive body of published data has appeared in the last few years, but the results often appear to be inconclusive or contradictory. In order to define the prognostic significance of DNA Cytometry in malignant diseases of the breast, large bowel, bladder, prostate, and hematopoietic system, and to clarify some of the technical issues related to clinical laboratory standards and quality controls, a DNA Cytometry Consensus Conference was held in Prout's Neck, Maine, on October 1-4, 1992. This meeting was sponsored by the NCI, the International Society for Analytical Cytology, and industry. The significance of the meeting's conclusions for clinical breast cancer are discussed here. The consensus statement regarding the clinical utility of DNA Cytometry in breast cancer, and the Guidelines for the Implementation of Clinical DNA Cytometry which were generated at this meeting, also appear in this issue of Breast Cancer Research and Treatment.
Robert E Cunningham - One of the best experts on this subject based on the ideXlab platform.
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Overview of flow Cytometry and fluorescent probes for flow Cytometry.
Methods in molecular biology (Clifton N.J.), 2010Co-Authors: Robert E CunninghamAbstract:This chapter provides an introduction to the use of fluorescent probes in flow Cytometry. Sample preparation for the use of surface labeling with antibodies as well as for the use of nucleic acid probes is discussed. The utility of cell sorting is also discussed.
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Overview of Flow Cytometry and Fluorescent Probes for Cytometry
Methods in molecular biology (Clifton N.J.), 1994Co-Authors: Robert E CunninghamAbstract:Abstract This chapter provides an introduction to the use of fluorescent probes in flow Cytometry. Sample preparation for the use of surface labeling with antibodies as well as for the use of nucleic acid probes is discussed. The utility of cell sorting is also discussed.
