The Experts below are selected from a list of 537 Experts worldwide ranked by ideXlab platform
Qing-yuan Sun - One of the best experts on this subject based on the ideXlab platform.
-
Interspecies nuclear transfer of Tibetan antelope using caprine oocyte as recipient.
Molecular reproduction and development, 2007Co-Authors: Zhen-jun Zhao, Heng-hua Cao, Quan-jun Zhang, Man-xi Jiang, Ying-chun Ouyang, Chang-long Nan, Zi-li Lei, Xiang-fen Song, Qing-yuan SunAbstract:Interspecies nuclear transfer is an invalulable tool for studying nucleus-cytoplasm interactions; and at the same time, it provides a possible alternative to clone endangered animals whose oocytes are difficult to obtain. In the present study, we investigated the possibility of cloning Tibetan antelope embryos using abattoir-derived caprine oocytes as recipients. Effects of culture conditions, enucleation timing, and donor cell passages on the in vitro development of Tibetan antelope-goat cloned embryos were studied. Maternal to zygotic transition timing of interspecies Tibetan antelope embryos was also investigated using two types of cloned embryos, Tibetan antelope-rabbit and Tibetan antelope-goat embryos. Our results indicate that: (1) goat oocyte is able to reprogram somatic cells of different genus and supports development to blastocyst in vitro. (2) Coculture system supported the development of Tibetan antelope-goat embryos to blastocyst rate stage (4.0%), while CR1aa alone did not. (3) When MII phase enucleated caprine Cytoplast and TII phase enucleated caprine Cytoplast were used as recipients, the fusion rate and blastocyst rate of hybrid embryos were not statistically different (73.9% vs. 67.4%; 4.0% vs. 1.1%). (4) When donor cells at 3-8 passages were used, 2.9% hybrid embryos developed to blastocysts, while none developed to blastocysts when cells at 10-17 passages were used. (5) There may be a morula-to-blastocyst block for Tibetan antelope-goat, while there may be an 8- to 16-cell block for Tibetan antelope-rabbit embryos.
-
rabbit oocyte cytoplasm supports development of nuclear transfer embryos derived from the somatic cells of the camel and tibetan antelope
Journal of Reproduction and Development, 2006Co-Authors: Zhen-jun Zhao, Ying-chun Ouyang, Chang-long Nan, Zi-li Lei, Xiang-fen Song, Qing-yuan Sun, Da-yuan ChenAbstract:This study was designed to examine the ability of rabbit metaphase II oocyte cytoplasm to support the development of interspecies nuclear transfer embryos reconstructed using donor nuclei from different species. Skin fibroblast cells from a camel and Tibetan antelope were used as donor nuclei. As a first step, we investigated the efficiency of different activation protocols by comparing the parthenogenetic development of rabbit oocytes. The protocol that yielded the highest blastocyst rate was used to activate the reconstructed embryos in nuclear transfer experiments. In addition, the effect of donor cell serum starvation on the development of the reconstructed embryo was also examined. More than half of the karyoplast-Cytoplast couplets could be fused, and about one third of the reconstructed embryos were capable of completing first cleavage, regardless of the species of donor nuclei. Some of the cleaving reconstructed embryos were even capable of progressing further and developing to the blastocyst stage (1.4-8.7% for the Tibetan antelope and 0-7.5% for the camel, respectively). Our results suggest that the mechanisms regulating early embryo development may be conserved among mammalian species and some factors existing in rabbit oocyte cytoplasm for somatic nucleus reprogramming and dedifferentiation may not be species-specific. Rabbit oocyte cytoplasm can reprogram donor nuclei regardless of the origin of the nucleus and support in vitro development to an advanced stage.
-
asynchronous Cytoplast and karyoplast transplantation reveals that the cytoplasm determines the developmental fate of the nucleus in mouse oocytes
Molecular Reproduction and Development, 2003Co-Authors: Yong Cheng, Qing-yuan Sun, Hengyu Fan, Duan Cheng Wen, Chao Tong, Zi Yu Zhu, Lei Lei, Da-yuan ChenAbstract:The relationship between nucleus and cytoplasm can be well revealed by nuclear transplantation. Here, we have investigated the behavior changes of the reconstructed oocytes after transferring the karyoplasts from mouse GV, MI, and MII oocytes into the Cytoplasts at the different developmental stages. When the GV Cytoplast was used as recipient and MI or MII karyoplast was used as donor (MI-GV pair and MII-GV pair), the reconstructed pairs extruded a polar body after electrofusion and culture. Both the cytoplasm and the polar body had a metaphase spindle in the MI-GV pair, while only a clutch of condensed chromatin was observed in the cytoplasm and polar body of the MII-GV pair. When the MI Cytoplast was used as recipient and GV or MII karyoplast was used as donor (GV-MI pair and MII-MI pair), the reconstructed pairs also extruded a polar body. Each had one spindle and a group of metaphase chromosomes in the cytoplasm and polar body, respectively. When the MII Cytoplast was used as recipient and GV or MI karyoplast was used as donor (GV-MII pair and MI-MII pair), the reconstructed pairs were activated, became parthenogenetic embryos and even developed to hatching blastocysts after electrofusion. The result from immunoblotting showed that MAP kinase activity was high in the MI and MII Cytoplasts, while not detected in GV Cytoplast. The results demonstrate that the cytoplasmic environment determines the behavior of asynchronous donors.
-
viable rabbits derived from reconstructed oocytes by germinal vesicle transfer after intracytoplasmic sperm injection icsi
Molecular Reproduction and Development, 2001Co-Authors: Da-yuan Chen, Jilong Liu, Qing-yuan Sun, Li Lian, Minkang Wang, Zhiming HanAbstract:Abnormal oocyte spindle due to the improper function of ooplasm is associated with female infertility of advanced maternal age. A possible way to overcome this problem is to transfer an oocyte germinal vesicle (GV) which contains genetic materials of a patient with a history of poor embryo development to the Cytoplast from a donor oocyte. Here we demonstrate that GV transfer is feasible using a rabbit model. When the GVs were transferred to auto- or hetero-Cytoplasts of GV stage oocytes, around 80% of the reconstructed oocytes could mature in vitro and 7.1-9.4% of the oocytes developed to blastocyst stage after intracytoplasmic sperm injection (ICSI). Transfer of 93 fertilized eggs reconstructed via GV transfer into six recipients resulted in two live offspring. Results of this experiment indicate that GV transfer can potentially become a new approach in treatment of infertility because of advanced maternal age.
Keith H S Campbell - One of the best experts on this subject based on the ideXlab platform.
-
KHS 2006 Effects of Enucleation and Caffeine on MaturationPromoting Factor (MPF) and Mitogen-Activated Protein Kinase (MAPK) Activities in Ovine Oocytes Used as Recipient Cytoplasts for Nuclear Transfer. Biology of Reproduction 74
2016Co-Authors: Joonhee Lee, Keith H S CampbellAbstract:In general, oocytes arrested atmetaphase of the secondmeiotic division (MII) are used as recipient Cytoplasts for nuclear transfer (NT) procedures. MII oocytes contain high levels of maturation-promoting factor (MPF) and mitogen-activated protein kinase (MAPK), which cause nuclear envelope breakdown (NEBD) and premature chromosome condensation (PCC) in the transferred nucleus and have been implicated in nuclear reprogramming. However, the occurrence of NEBD and the extent of PCC are variable between individual oocytes and species and are dependent on donor cell type and cell cycle stage. Enucleation, which removes oocyte cytoplasm, may reduce MPF and MAPK activities and reduce reprogramming; conversely, increasing kinase activities may increase reprogramming. We compared the effects of enucleation of ovine oocytes at anaphase/telophase of the first meiotic division (AI-TI) and at MII. MPF and MAPK activities were maximal at MII; blind enucleation at AI-TI was more efficient than at MII and removed a smaller volume of cytoplasm. Neither protocol significantly affected the activity of either kinase and the fate of the donor nucleus; however, enucleation per se significantly reduced the occurrence of NEBD in NT embryos. Treatment with 10 mM caffeine significantly increased the activities of both kinases and the occurrence of NEBD but did not affect the frequency of development to the blastocyst stage; however, a significant increase in total cell numbers was observed. The results show that caffeine can increase MPF and MAPK activities in ovine oocytes and that this may contribute to an increased reprogramming in NT embryos. embryo, kinases, meiosis, oocyte development, phosphatase
-
nuclear cytoplasmic incompatibility and inefficient development of pig mouse cytoplasmic hybrid embryos
Reproduction, 2011Co-Authors: Dasari Amarnath, Inchul Choi, Adel R Moawad, Teruhiko Wakayama, Keith H S CampbellAbstract:Inter-species somatic cell nuclear transfer (iSCNT) embryos usually fail to develop to the blastocyst stage and beyond due to incomplete reprogramming of donor cell. We evaluated whether using a karyoplast that would require less extensive reprogramming such as an embryonic blastomere or the meiotic spindle from metaphase II oocytes would provide additional insight into the development of iSCNT embryos. Our results showed that karyoplasts of embryonic or oocyte origin are no different from somatic cells; all iSCNT embryos, irrespective of karyoplast origin, were arrested during early development. We hypothesized that nuclear-cytoplasmic incompatibility could be another reason for failure of embryonic development from iSCNT. We used pig-mouse cytoplasmic hybrids as a model to address nuclear-cytoplasmic incompatibility in iSCNT embryos. Fertilized murine zygotes were reconstructed by fusing with porcine Cytoplasts of varying cytoplasmic volumes (1/10 (small) and 1/5 (large) total volume of mouse zygote). The presence of pig cytoplasm significantly reduced the development of mouse zygotes to the blastocyst stage compared with control embryos at 120 h post-human chorionic gondotropin (41 vs 6 vs 94%, P<0.05; 1/10, 1/5, control respectively). While mitochondrial DNA copy numbers remained relatively unchanged, expression of several important genes namely Tfam, Polg, Polg2, Mfn2, Slc2a3 (Glut3), Slc2a1 (Glut1), Bcl2, Hspb1, Pou5f1 (Oct4), Nanog, Cdx2, Gata3, Tcfap2c, mt-Cox1 and mt-Cox2 was significantly reduced in cytoplasmic hybrids compared with control embryos. These results demonstrate that the presence of even a small amount of porcine cytoplasm is detrimental to murine embryo development and suggest that a range of factors are likely to contribute to the failure of inter-species nuclear transfer embryos.
-
effects of enucleation and caffeine on maturation promoting factor mpf and mitogen activated protein kinase mapk activities in ovine oocytes used as recipient Cytoplasts for nuclear transfer
Biology of Reproduction, 2006Co-Authors: Joonhee Lee, Keith H S CampbellAbstract:In general, oocytes arrested at metaphase of the second meiotic division (MII) are used as recipient Cytoplasts for nuclear transfer (NT) procedures. MII oocytes contain high levels of maturation-promoting factor (MPF) and mitogen-activated protein kinase (MAPK), which cause nuclear envelope breakdown (NEBD) and premature chromosome condensation (PCC) in the transferred nucleus and have been implicated in nuclear reprogramming. However, the occurrence of NEBD and the extent of PCC are variable between individual oocytes and species and are dependent on donor cell type and cell cycle stage. Enucleation, which removes oocyte cytoplasm, may reduce MPF and MAPK activities and reduce reprogramming; conversely, increasing kinase activities may increase reprogramming. We compared the effects of enucleation of ovine oocytes at anaphase/telophase of the first meiotic division (AI-TI) and at MII. MPF and MAPK activities were maximal at MII; blind enucleation at AI-TI was more efficient than at MII and removed a smaller volume of cytoplasm. Neither protocol significantly affected the activity of either kinase and the fate of the donor nucleus; however, enucleation per se significantly reduced the occurrence of NEBD in NT embryos. Treatment with 10 mM caffeine significantly increased the activities of both kinases and the occurrence of NEBD but did not affect the frequency of development to the blastocyst stage; however, a significant increase in total cell numbers was observed. The results show that caffeine can increase MPF and MAPK activities in ovine oocytes and that this may contribute to an increased reprogramming in NT embryos.
D N Wells - One of the best experts on this subject based on the ideXlab platform.
-
28 doubling oocyte cytoplasm volume increases blastocyst quality following interspecies somatic cell nuclear transfer in argali sheep ovis ammon
Reproduction Fertility and Development, 2016Co-Authors: Andria Green, F C Oback, J E Oliver, L Popovic, L T Mcgowan, S J Appleby, Fanli Meng, D L Hyndman, D Carson, D N WellsAbstract:Interspecies somatic cell NT (SCNT) can be used in the conservation of endangered animals but only when there is an abundant source of compatible oocytes and recipients. The objective of this study was to compare the efficiency of intra- and interspecies SCNT in sheep using zona-free embryo reconstruction methods. Skin fibroblasts from either an argali (Ovis ammon) or control (Ovis aries) ram were used as donor cells for SCNT between passages 2 to 5 and following culture in medium containing 0.5% FCS for 4 to 6 days. Single cells were electrically fused to Cytoplasts prepared following enucleation of in vitro-matured zona-free metaphase II-arrested oocytes obtained from domestic ewes. In an additional experiment with argali, a double Cytoplast (DC-SCNT) procedure was used whereby a second Cytoplast was fused to the first reconstruct within 1 h. Reconstructs were artificially activated ~25 h after the start of maturation using ionomycin and 6-DMAP. Zona-free parthenogenote (PG) control oocytes were activated around the same time. In each treatment, 10 to 12 zona-free embryos where cultured in microwells formed in 20-μL drops of modified synthetic oviduct fluid under oil. Half the medium was replaced on Day 3, and developing embryos were transferred to individual 5-μL drops on Day 6. Development on Day 7 was expressed as a percentage of cleaved embryos. Statistical significance was determined using Fisher’s exact test for embryo development and two-tailed t-test for embryo cell numbers. Total embryo development on Day 7 was significantly greater with intraspecies sheep SCNT compared with interspecies argali SCNT (34/157 = 21.7% v. 34/363 = 9.4%, respectively; P < 0.001). Moreover, SCNT embryo development was significantly less than PG controls (122/360 = 33.9%; P < 0.001). Although argali DC-SCNT had no effect on total embryo development compared with SCNT (9/69 = 13.0% v. 7/56 = 12.5%, respectively), doubling cytoplasm volume increased the proportion of grade 1 and 2 embryos on Day 7 (8.7 v. 0%; P < 0.05). Consequently, DC-SCNT blastocysts had greater numbers of nuclei compared with SCNT (108 ± 47 v. 65 ± 9; n = 6, n = 6, respectively; P = 0.054). In comparison, PG blastocysts possessed on average 122 ± 27 nuclei (n = 7). A sample of 14 argali cloned blastocysts were all confirmed to have been derived from the respective ram after genotyping ~6000 ovine single nucleotide polymorphisms on a custom-made chip (Illumina, San Diego, CA, USA). Chromosome spreads of argali embryos revealed a modal number of 4 bi-armed autosomes as opposed to 6 in Ovis aries. In conclusion, in vitro development following interspecies SCNT in argali was about half that compared with domestic sheep. Blastocyst quality was improved by increasing oocyte cytoplasmic volume facilitated by zona-free cloning. Alternative sources of cytoplasm may further improve development. Confirmation that Ovis aries cytoplasm can fully reprogram a differentiated argali nucleus remains to be determined. This research was supported by AgResearch Core Funding.
-
production of cloned calves following nuclear transfer with cultured adult mural granulosa cells
Biology of Reproduction, 1999Co-Authors: D N Wells, P M Misica, H R TervitAbstract:Adult somatic cell nuclear transfer was used to determine the totipotent potential of cultured mural granulosa cells, obtained from a Friesian dairy cow of high genetic merit. Nuclei were exposed to oocyte cytoplasm for prolonged periods by electrically fusing quiescent cultured cells to enucleated metaphase II Cytoplasts 4-6 h before activation (fusion before activation [FBA] treatment). Additionally, some first-generation morulae were recloned by fusing blastomeres to S-phase Cytoplasts. A significantly higher proportion of fused embryos developed in vitro to grade 1-2 blastocysts on Day 7 with FBA (27.5 +/- 2.5%) than with recloning (13.0 +/- 3.6%; p < 0. 05). After the transfer of 100 blastocysts from the FBA treatment, survival rates on Days 60, 100, 180, and term were 45%, 21%, 17%, and 10%, respectively. Ten heifer calves were delivered by elective cesarean section; all have survived. After the transfer of 16 recloned blastocysts, embryo survival on Day 60 was 38%; however, no fetuses survived to Day 100. DNA analyses confirmed that the calves are all genetically identical to the donor cow. It is suggested that the losses throughout gestation may in part be due to placental dysfunction at specific stages. The next advance in this technology will be to introduce specific genetic modifications of biomedical or agricultural interest.
-
production of cloned lambs from an established embryonic cell line a comparison between in vivo and in vitro matured Cytoplasts
Biology of Reproduction, 1997Co-Authors: D N Wells, P M Misica, H R TervitAbstract:Nuclear transfer procedures were used to determine the in vivo developmental potential of an ovine embryonic cell line isolated from the inner cell mass of a Day 8 blastocyst-stage embryo. This cell line possessed a differentiated epithelial-like cell morphology. In this study, a comparison was made between in vivo- and in vitro-derived oocytes used as recipient Cytoplasts in the nuclear transfer procedure. Cultured cells were induced to quiesce and enter presumptive GO before being used as donor karyoplasts between passages 8 and 16 of culture. After cell fusion, reconstructed embryos were cultured for 6 days in vitro in embryo culture medium. Blastocyst-stage embryos were subsequently transferred to synchronized recipient ewes (n = 37), and development was allowed to proceed to term. There was a significant effect of source of recipient Cytoplast, with development being consistently greater with in vivo compared to in vitro Cytoplasts in terms of, respectively, blastocysts produced (24.2 3.8% vs. 17.1 ± 2.3%; p = 0.1), Day 35 pregnancy rate (40.0% vs. 9.1 %; p < 0.05), and Day 35 embryo survival (19.4% vs. 4.5%; p < 0.05). A high proportion of fetuses died during late gestation (5 of 8). The major abnormalities were associated with the urogenital tract. However, three lambs were delivered alive following cesarean section on Day 147. One lamb, derived from an in vitro-matured oocyte, died after 10 min, while the remaining two from in vivo-ovulated oocytes are apparently normal and healthy. DNA microsatellite markers conclusively show that the three lambs are genetically identical and were derived from the embryonic cell line. In conclusion, some cells from this blastocyst-derived embryonic cell line are totipotent by nuclear transfer and can produce viable offspring.
Yungkuan Chan - One of the best experts on this subject based on the ideXlab platform.
-
nucleus and Cytoplast contour detector from a cervical smear image
Expert Systems With Applications, 2012Co-Authors: Peiyan Pai, Chinchen Chang, Yungkuan ChanAbstract:In this paper, a nucleus and Cytoplast contour detector (NCC detector) is presented to automatically detect the Cytoplast and nucleus contours of a cell in a cervical smear image. The NCC detector uses the adaptable threshold decision (ATD) method to separate the cell from the cervical smear image, and then uses the maximal gray-level-gradient-difference (MGLGD) method, proposed in this paper, to extract the nucleus from the cell. The experimental results show that the NCC detector is superior to two existing methods, the gradient vector flow-active contour model (GVF-ACM) and the edge enhancement nucleus and Cytoplast contour (ENNCC) detector, in segmenting the Cytoplast and nucleus of a cell.
-
detection and segmentation of cervical cell Cytoplast and nucleus
International Journal of Imaging Systems and Technology, 2009Co-Authors: Chuenhorng Lin, Yungkuan Chan, Chunchieh ChenAbstract:This article aims to develop a method for the detection and segmentation of a Cytoplast and nucleus from a cervix smear image. First, the technique of equalization method with Gaussian filter is adopted to eliminate noise in the image. Second, a new edge enhancement technique is proposed to work out the coarseness of each pixel, which is later used as a determining characteristic of reinforced object images. A two-group object enhancement technique is then used to reinforce this object according to rough pixels. Third, the proposed detector enhances the gradients of the edges of the Cytoplast and nucleus while suppressing the noise gradients, and then specifies the pixels with higher gradients as possible edge pixels. Finally, it picks out the two longest closed curves constructed by part of the edge pixels. Detection and segmentation performance of the proposed method is later compared with seed region growing feature extraction and level set method using 10 cervix smear images as example. Besides comparing the contour segment of the Cytoplast and nucleus obtained by using different methods, we also compare the quality of the segmentation results. Experimental results show that the proposed detector demonstrates an impressive performance. © 2009 Wiley Periodicals, Inc. Int J Imaging Syst Technol, 19, 260–270, 2009
-
edge enhancement nucleus and Cytoplast contour detector of cervical smear images
Systems Man and Cybernetics, 2008Co-Authors: Shysfan Yangmao, Yungkuan Chan, Yenping ChuAbstract:This paper presents an edge enhancement nucleus and Cytoplast contour (EENCC) detector to enable cutting the nucleus and Cytoplast from a cervical smear cell image. To clean up noises from an image, this paper proposes a trim-meaning filter that can effectively remove impulse and Gaussian noises but still preserves the sharpness of object boundaries. In addition, a bigroup enhancer is proposed to make a clear-cut separation of the pixels lying in-between two objects. A mean vector difference enhancer is presented to suppress the gradients of noises and also to brighten the gradients of object contours. What is more, a relative-distance-error measure is put forward to evaluate the segmentation error between the extracted and target object contours. The experimental results show that all the aforementioned techniques proposed have performed impressively. Other than for cervical smear images, these proposed techniques can also be utilized in object segmentation of other images.
Da-yuan Chen - One of the best experts on this subject based on the ideXlab platform.
-
rabbit oocyte cytoplasm supports development of nuclear transfer embryos derived from the somatic cells of the camel and tibetan antelope
Journal of Reproduction and Development, 2006Co-Authors: Zhen-jun Zhao, Ying-chun Ouyang, Chang-long Nan, Zi-li Lei, Xiang-fen Song, Qing-yuan Sun, Da-yuan ChenAbstract:This study was designed to examine the ability of rabbit metaphase II oocyte cytoplasm to support the development of interspecies nuclear transfer embryos reconstructed using donor nuclei from different species. Skin fibroblast cells from a camel and Tibetan antelope were used as donor nuclei. As a first step, we investigated the efficiency of different activation protocols by comparing the parthenogenetic development of rabbit oocytes. The protocol that yielded the highest blastocyst rate was used to activate the reconstructed embryos in nuclear transfer experiments. In addition, the effect of donor cell serum starvation on the development of the reconstructed embryo was also examined. More than half of the karyoplast-Cytoplast couplets could be fused, and about one third of the reconstructed embryos were capable of completing first cleavage, regardless of the species of donor nuclei. Some of the cleaving reconstructed embryos were even capable of progressing further and developing to the blastocyst stage (1.4-8.7% for the Tibetan antelope and 0-7.5% for the camel, respectively). Our results suggest that the mechanisms regulating early embryo development may be conserved among mammalian species and some factors existing in rabbit oocyte cytoplasm for somatic nucleus reprogramming and dedifferentiation may not be species-specific. Rabbit oocyte cytoplasm can reprogram donor nuclei regardless of the origin of the nucleus and support in vitro development to an advanced stage.
-
asynchronous Cytoplast and karyoplast transplantation reveals that the cytoplasm determines the developmental fate of the nucleus in mouse oocytes
Molecular Reproduction and Development, 2003Co-Authors: Yong Cheng, Qing-yuan Sun, Hengyu Fan, Duan Cheng Wen, Chao Tong, Zi Yu Zhu, Lei Lei, Da-yuan ChenAbstract:The relationship between nucleus and cytoplasm can be well revealed by nuclear transplantation. Here, we have investigated the behavior changes of the reconstructed oocytes after transferring the karyoplasts from mouse GV, MI, and MII oocytes into the Cytoplasts at the different developmental stages. When the GV Cytoplast was used as recipient and MI or MII karyoplast was used as donor (MI-GV pair and MII-GV pair), the reconstructed pairs extruded a polar body after electrofusion and culture. Both the cytoplasm and the polar body had a metaphase spindle in the MI-GV pair, while only a clutch of condensed chromatin was observed in the cytoplasm and polar body of the MII-GV pair. When the MI Cytoplast was used as recipient and GV or MII karyoplast was used as donor (GV-MI pair and MII-MI pair), the reconstructed pairs also extruded a polar body. Each had one spindle and a group of metaphase chromosomes in the cytoplasm and polar body, respectively. When the MII Cytoplast was used as recipient and GV or MI karyoplast was used as donor (GV-MII pair and MI-MII pair), the reconstructed pairs were activated, became parthenogenetic embryos and even developed to hatching blastocysts after electrofusion. The result from immunoblotting showed that MAP kinase activity was high in the MI and MII Cytoplasts, while not detected in GV Cytoplast. The results demonstrate that the cytoplasmic environment determines the behavior of asynchronous donors.
-
viable rabbits derived from reconstructed oocytes by germinal vesicle transfer after intracytoplasmic sperm injection icsi
Molecular Reproduction and Development, 2001Co-Authors: Da-yuan Chen, Jilong Liu, Qing-yuan Sun, Li Lian, Minkang Wang, Zhiming HanAbstract:Abnormal oocyte spindle due to the improper function of ooplasm is associated with female infertility of advanced maternal age. A possible way to overcome this problem is to transfer an oocyte germinal vesicle (GV) which contains genetic materials of a patient with a history of poor embryo development to the Cytoplast from a donor oocyte. Here we demonstrate that GV transfer is feasible using a rabbit model. When the GVs were transferred to auto- or hetero-Cytoplasts of GV stage oocytes, around 80% of the reconstructed oocytes could mature in vitro and 7.1-9.4% of the oocytes developed to blastocyst stage after intracytoplasmic sperm injection (ICSI). Transfer of 93 fertilized eggs reconstructed via GV transfer into six recipients resulted in two live offspring. Results of this experiment indicate that GV transfer can potentially become a new approach in treatment of infertility because of advanced maternal age.
