The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Yves Longtin - One of the best experts on this subject based on the ideXlab platform.
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correlation between clostridium difficile bacterial load commercial real time pcr cycle thresholds and results of diagnostic tests based on enzyme immunoAssay and cell culture Cytotoxicity Assay
Journal of Clinical Microbiology, 2013Co-Authors: Lealaurence Dionne, Frederic Raymond, Jacques Corbeil, Jean Longtin, Philippe Gervais, Yves LongtinAbstract:ABSTRACT The impact of Clostridium difficile fecal loads on diagnostic test results is poorly understood, but it may have clinical importance. In this study, we investigated the relationship between C. difficile fecal load and the results of four Assays: a glutamate dehydrogenase (GDH) enzyme immunoAssay (EIA), a toxin A/B antigen EIA (ToxAB), a cell culture Cytotoxicity Assay (CCA), and PCR targeting the tcdB gene. We also compared the PCR cycle threshold (CT) with the results of quantitative culture using Spearman9s rank correlation coefficient. Finally, we sequenced the genomes of 24 strains with different detection profiles. A total of 203 clinical samples harboring toxigenic C. difficile were analyzed and sorted into one of four groups: 17 PCR+ (group 1), 37 PCR+ GDH+ (group 2), 24 PCR+ GDH+ CCA+ (group 3), and 125 PCR+ GDH+ ToxAB+ (group 4). The overall median fecal load in log10 CFU/g was 6.67 (interquartile range [IQR], 5.57 to 7.54). The median fecal bacterial load of groups 1, 2, 3, and 4 were 4.15 (IQR, 3.00 to 4.98), 5.74 (IQR, 4.75 to 6.16), 6.20 (IQR, 5.23 to 6.80), and 7.08 (IQR, 6.35 to 7.83), respectively. Group 1 samples had lower fecal loads than those from each of the other groups (P
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correlation between clostridium difficile bacterial load commercial real time pcr cycle thresholds and results of diagnostic tests based on enzyme immunoAssay and cell culture Cytotoxicity Assay
Journal of Clinical Microbiology, 2013Co-Authors: Lealaurence Dionne, Frederic Raymond, Jacques Corbeil, Jean Longtin, Philippe Gervais, Yves LongtinAbstract:ABSTRACT The impact of Clostridium difficile fecal loads on diagnostic test results is poorly understood, but it may have clinical importance. In this study, we investigated the relationship between C. difficile fecal load and the results of four Assays: a glutamate dehydrogenase (GDH) enzyme immunoAssay (EIA), a toxin A/B antigen EIA (ToxAB), a cell culture Cytotoxicity Assay (CCA), and PCR targeting the tcdB gene. We also compared the PCR cycle threshold ( C T ) with the results of quantitative culture using Spearman9s rank correlation coefficient. Finally, we sequenced the genomes of 24 strains with different detection profiles. A total of 203 clinical samples harboring toxigenic C. difficile were analyzed and sorted into one of four groups: 17 PCR + (group 1), 37 PCR + GDH + (group 2), 24 PCR + GDH + CCA + (group 3), and 125 PCR + GDH + ToxAB + (group 4). The overall median fecal load in log 10 CFU/g was 6.67 (interquartile range [IQR], 5.57 to 7.54). The median fecal bacterial load of groups 1, 2, 3, and 4 were 4.15 (IQR, 3.00 to 4.98), 5.74 (IQR, 4.75 to 6.16), 6.20 (IQR, 5.23 to 6.80), and 7.08 (IQR, 6.35 to 7.83), respectively. Group 1 samples had lower fecal loads than those from each of the other groups ( P P C T and fecal loads (ρ = −0.697; P P = 0.041). This study demonstrates an association between C. difficile fecal load and the results of routinely used diagnostic tests.
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correlation between clostridium difficile bacterial load commercial real time pcr cycle thresholds and results of diagnostic tests based on enzyme immunoAssay and cell culture Cytotoxicity Assay
Journal of Clinical Microbiology, 2013Co-Authors: Lealaurence Dionne, Frederic Raymond, Jacques Corbeil, Jean Longtin, Philippe Gervais, Yves LongtinAbstract:The impact of Clostridium difficile fecal loads on diagnostic test results is poorly understood, but it may have clinical importance. In this study, we investigated the relationship between C. difficile fecal load and the results of four Assays: a glutamate dehydrogenase (GDH) enzyme immunoAssay (EIA), a toxin A/B antigen EIA (ToxAB), a cell culture Cytotoxicity Assay (CCA), and PCR targeting the tcdB gene. We also compared the PCR cycle threshold (CT) with the results of quantitative culture using Spearman's rank correlation coefficient. Finally, we sequenced the genomes of 24 strains with different detection profiles. A total of 203 clinical samples harboring toxigenic C. difficile were analyzed and sorted into one of four groups: 17 PCR+ (group 1), 37 PCR+ GDH+ (group 2), 24 PCR+ GDH+ CCA+ (group 3), and 125 PCR+ GDH+ ToxAB+ (group 4). The overall median fecal load in log10 CFU/g was 6.67 (interquartile range [IQR], 5.57 to 7.54). The median fecal bacterial load of groups 1, 2, 3, and 4 were 4.15 (IQR, 3.00 to 4.98), 5.74 (IQR, 4.75 to 6.16), 6.20 (IQR, 5.23 to 6.80), and 7.08 (IQR, 6.35 to 7.83), respectively. Group 1 samples had lower fecal loads than those from each of the other groups (P < 0.001). Group 2 samples had lower fecal loads than those from groups 3 and 4 (P < 0.001). There was a significant correlation between PCR CT and fecal loads (ρ = −0.697; P < 0.001). NAP1 strains were associated with the detection of toxins by EIA or CCA (P = 0.041). This study demonstrates an association between C. difficile fecal load and the results of routinely used diagnostic tests.
Lealaurence Dionne - One of the best experts on this subject based on the ideXlab platform.
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correlation between clostridium difficile bacterial load commercial real time pcr cycle thresholds and results of diagnostic tests based on enzyme immunoAssay and cell culture Cytotoxicity Assay
Journal of Clinical Microbiology, 2013Co-Authors: Lealaurence Dionne, Frederic Raymond, Jacques Corbeil, Jean Longtin, Philippe Gervais, Yves LongtinAbstract:ABSTRACT The impact of Clostridium difficile fecal loads on diagnostic test results is poorly understood, but it may have clinical importance. In this study, we investigated the relationship between C. difficile fecal load and the results of four Assays: a glutamate dehydrogenase (GDH) enzyme immunoAssay (EIA), a toxin A/B antigen EIA (ToxAB), a cell culture Cytotoxicity Assay (CCA), and PCR targeting the tcdB gene. We also compared the PCR cycle threshold (CT) with the results of quantitative culture using Spearman9s rank correlation coefficient. Finally, we sequenced the genomes of 24 strains with different detection profiles. A total of 203 clinical samples harboring toxigenic C. difficile were analyzed and sorted into one of four groups: 17 PCR+ (group 1), 37 PCR+ GDH+ (group 2), 24 PCR+ GDH+ CCA+ (group 3), and 125 PCR+ GDH+ ToxAB+ (group 4). The overall median fecal load in log10 CFU/g was 6.67 (interquartile range [IQR], 5.57 to 7.54). The median fecal bacterial load of groups 1, 2, 3, and 4 were 4.15 (IQR, 3.00 to 4.98), 5.74 (IQR, 4.75 to 6.16), 6.20 (IQR, 5.23 to 6.80), and 7.08 (IQR, 6.35 to 7.83), respectively. Group 1 samples had lower fecal loads than those from each of the other groups (P
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correlation between clostridium difficile bacterial load commercial real time pcr cycle thresholds and results of diagnostic tests based on enzyme immunoAssay and cell culture Cytotoxicity Assay
Journal of Clinical Microbiology, 2013Co-Authors: Lealaurence Dionne, Frederic Raymond, Jacques Corbeil, Jean Longtin, Philippe Gervais, Yves LongtinAbstract:ABSTRACT The impact of Clostridium difficile fecal loads on diagnostic test results is poorly understood, but it may have clinical importance. In this study, we investigated the relationship between C. difficile fecal load and the results of four Assays: a glutamate dehydrogenase (GDH) enzyme immunoAssay (EIA), a toxin A/B antigen EIA (ToxAB), a cell culture Cytotoxicity Assay (CCA), and PCR targeting the tcdB gene. We also compared the PCR cycle threshold ( C T ) with the results of quantitative culture using Spearman9s rank correlation coefficient. Finally, we sequenced the genomes of 24 strains with different detection profiles. A total of 203 clinical samples harboring toxigenic C. difficile were analyzed and sorted into one of four groups: 17 PCR + (group 1), 37 PCR + GDH + (group 2), 24 PCR + GDH + CCA + (group 3), and 125 PCR + GDH + ToxAB + (group 4). The overall median fecal load in log 10 CFU/g was 6.67 (interquartile range [IQR], 5.57 to 7.54). The median fecal bacterial load of groups 1, 2, 3, and 4 were 4.15 (IQR, 3.00 to 4.98), 5.74 (IQR, 4.75 to 6.16), 6.20 (IQR, 5.23 to 6.80), and 7.08 (IQR, 6.35 to 7.83), respectively. Group 1 samples had lower fecal loads than those from each of the other groups ( P P C T and fecal loads (ρ = −0.697; P P = 0.041). This study demonstrates an association between C. difficile fecal load and the results of routinely used diagnostic tests.
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correlation between clostridium difficile bacterial load commercial real time pcr cycle thresholds and results of diagnostic tests based on enzyme immunoAssay and cell culture Cytotoxicity Assay
Journal of Clinical Microbiology, 2013Co-Authors: Lealaurence Dionne, Frederic Raymond, Jacques Corbeil, Jean Longtin, Philippe Gervais, Yves LongtinAbstract:The impact of Clostridium difficile fecal loads on diagnostic test results is poorly understood, but it may have clinical importance. In this study, we investigated the relationship between C. difficile fecal load and the results of four Assays: a glutamate dehydrogenase (GDH) enzyme immunoAssay (EIA), a toxin A/B antigen EIA (ToxAB), a cell culture Cytotoxicity Assay (CCA), and PCR targeting the tcdB gene. We also compared the PCR cycle threshold (CT) with the results of quantitative culture using Spearman's rank correlation coefficient. Finally, we sequenced the genomes of 24 strains with different detection profiles. A total of 203 clinical samples harboring toxigenic C. difficile were analyzed and sorted into one of four groups: 17 PCR+ (group 1), 37 PCR+ GDH+ (group 2), 24 PCR+ GDH+ CCA+ (group 3), and 125 PCR+ GDH+ ToxAB+ (group 4). The overall median fecal load in log10 CFU/g was 6.67 (interquartile range [IQR], 5.57 to 7.54). The median fecal bacterial load of groups 1, 2, 3, and 4 were 4.15 (IQR, 3.00 to 4.98), 5.74 (IQR, 4.75 to 6.16), 6.20 (IQR, 5.23 to 6.80), and 7.08 (IQR, 6.35 to 7.83), respectively. Group 1 samples had lower fecal loads than those from each of the other groups (P < 0.001). Group 2 samples had lower fecal loads than those from groups 3 and 4 (P < 0.001). There was a significant correlation between PCR CT and fecal loads (ρ = −0.697; P < 0.001). NAP1 strains were associated with the detection of toxins by EIA or CCA (P = 0.041). This study demonstrates an association between C. difficile fecal load and the results of routinely used diagnostic tests.
Karen C Carroll - One of the best experts on this subject based on the ideXlab platform.
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frequency of sample submission for optimal utilization of the cell culture Cytotoxicity Assay for detection of clostridium difficile toxin
Journal of Clinical Microbiology, 2005Co-Authors: Anita P Borek, Deborah Aird, Karen C CarrollAbstract:We reviewed the results of repeated sample submissions within a 7-day time frame for Clostridium difficile toxin testing. A total of 2,940 samples were tested during a 3-month period using a cell culture Cytotoxicity Assay (CCCA). The results from all second samples (n = 1,101) were concordant with the original test result. In only two cases (0.8%; n = 247) was a third sample positive when the first two samples were negative. In this study, submission of multiple samples for CCCA did not increase detection of Clostridium difficile infection.
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comparison of the tox a b test to a cell culture Cytotoxicity Assay for the detection of clostridium difficile in stools
Diagnostic Microbiology and Infectious Disease, 2000Co-Authors: William E Aldeen, M Bingham, A Aiderzada, J Kucera, S Jense, Karen C CarrollAbstract:The TOX A/B Test (Techlab, Blacksburg, VA, USA) was compared to cell culture Cytotoxicity Assay on 1109 consecutive diarrheal stool samples collected from patients with the presumptive diagnosis of Clostridium difficile disease. The TOX A/B Test is an enzyme immunoAssay in a microtiter format that detects both toxins A and B. The procedure used for this study takes approximately 1.5 h to perform. Cell culture Cytotoxicity was performed by using a fibroblast cell line in a microtiter format read at 4 h, 24 h, and 48 h. One hundred ninety-four of the 1109 samples were positive by the "gold standard" Cytotoxicity Assay, whereas 189 were positive by EIA. There was a 98.5% agreement between the two Assays. When compared to the Cytotoxicity Assay, the EIA had an initial sensitivity of 94.3% and a specificity of 99.3%. However, after resolution of six discrepants using another ELISA for toxin A detection the sensitivity, specificity, positive and negative predictive values for the TOX A/B test are as follows: 94.5%; 100%; 100%; 98.8%. The corresponding values for the Cytotoxicity Assay are: 97%; 100%; 100%; and 99.3%. This test seems to have excellent sensitivity and specificity as compared to an in-house cell culture Cytotoxicity Assay. It is sensitive enough to use as a stand-alone test for the detection of C. difficile toxin in laboratories that do not have cell culture Cytotoxicity testing capability.
Arun K. Bhunia - One of the best experts on this subject based on the ideXlab platform.
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WST-1-based cell Cytotoxicity Assay as a substitute for MTT-based Assay for rapid detection of toxigenic Bacillus species using CHO cell line.
Journal of microbiological methods, 2008Co-Authors: Puriya Ngamwongsatit, Padmapriya P. Banada, Watanalai Panbangred, Arun K. BhuniaAbstract:Bacillus cereus continues to be one of the important foodborne pathogens due to its ability to produce various heat-labile and -stable toxins. Several methods have been developed to assess the pathogenicity of the B. cereus strains; however, most of these take more than 2-3 days to provide confirmatory results. In this study we standardized a one-step Cytotoxicity Assay using WST-1 (4-[3-(4-iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]-1,3-benzene disulfonate) and compared with the traditional MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide)-based Assay for rapid detection of cytotoxic Bacillus spp. using Chinese hamster ovary (CHO) cell line. Crude toxin preparations from 50 isolates of Bacillus spp. were exposed to CHO cell line for 1 h or 24 h and the Cytotoxicity was determined by using WST-1 and MTT-based methods. Most B. cereus strains and some strains of other Bacillus species from our collection or from food sources showed comparably high Cytotoxicity using either of the methods (P=0.81); however, WST-1 Assay provided results in only 3 h while MTT Assay in 44-52 h. A positive correlation (R2=0.93) between WST-1 and MTT Assays strongly suggests that the WST-1-based Cytotoxicity Assay could be used as an alternative method to MTT Assay for rapid (3 h) confirmation of toxigenic Bacillus species in foods prior to their retail distribution or consumption.
Philippe Gervais - One of the best experts on this subject based on the ideXlab platform.
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correlation between clostridium difficile bacterial load commercial real time pcr cycle thresholds and results of diagnostic tests based on enzyme immunoAssay and cell culture Cytotoxicity Assay
Journal of Clinical Microbiology, 2013Co-Authors: Lealaurence Dionne, Frederic Raymond, Jacques Corbeil, Jean Longtin, Philippe Gervais, Yves LongtinAbstract:ABSTRACT The impact of Clostridium difficile fecal loads on diagnostic test results is poorly understood, but it may have clinical importance. In this study, we investigated the relationship between C. difficile fecal load and the results of four Assays: a glutamate dehydrogenase (GDH) enzyme immunoAssay (EIA), a toxin A/B antigen EIA (ToxAB), a cell culture Cytotoxicity Assay (CCA), and PCR targeting the tcdB gene. We also compared the PCR cycle threshold (CT) with the results of quantitative culture using Spearman9s rank correlation coefficient. Finally, we sequenced the genomes of 24 strains with different detection profiles. A total of 203 clinical samples harboring toxigenic C. difficile were analyzed and sorted into one of four groups: 17 PCR+ (group 1), 37 PCR+ GDH+ (group 2), 24 PCR+ GDH+ CCA+ (group 3), and 125 PCR+ GDH+ ToxAB+ (group 4). The overall median fecal load in log10 CFU/g was 6.67 (interquartile range [IQR], 5.57 to 7.54). The median fecal bacterial load of groups 1, 2, 3, and 4 were 4.15 (IQR, 3.00 to 4.98), 5.74 (IQR, 4.75 to 6.16), 6.20 (IQR, 5.23 to 6.80), and 7.08 (IQR, 6.35 to 7.83), respectively. Group 1 samples had lower fecal loads than those from each of the other groups (P
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correlation between clostridium difficile bacterial load commercial real time pcr cycle thresholds and results of diagnostic tests based on enzyme immunoAssay and cell culture Cytotoxicity Assay
Journal of Clinical Microbiology, 2013Co-Authors: Lealaurence Dionne, Frederic Raymond, Jacques Corbeil, Jean Longtin, Philippe Gervais, Yves LongtinAbstract:ABSTRACT The impact of Clostridium difficile fecal loads on diagnostic test results is poorly understood, but it may have clinical importance. In this study, we investigated the relationship between C. difficile fecal load and the results of four Assays: a glutamate dehydrogenase (GDH) enzyme immunoAssay (EIA), a toxin A/B antigen EIA (ToxAB), a cell culture Cytotoxicity Assay (CCA), and PCR targeting the tcdB gene. We also compared the PCR cycle threshold ( C T ) with the results of quantitative culture using Spearman9s rank correlation coefficient. Finally, we sequenced the genomes of 24 strains with different detection profiles. A total of 203 clinical samples harboring toxigenic C. difficile were analyzed and sorted into one of four groups: 17 PCR + (group 1), 37 PCR + GDH + (group 2), 24 PCR + GDH + CCA + (group 3), and 125 PCR + GDH + ToxAB + (group 4). The overall median fecal load in log 10 CFU/g was 6.67 (interquartile range [IQR], 5.57 to 7.54). The median fecal bacterial load of groups 1, 2, 3, and 4 were 4.15 (IQR, 3.00 to 4.98), 5.74 (IQR, 4.75 to 6.16), 6.20 (IQR, 5.23 to 6.80), and 7.08 (IQR, 6.35 to 7.83), respectively. Group 1 samples had lower fecal loads than those from each of the other groups ( P P C T and fecal loads (ρ = −0.697; P P = 0.041). This study demonstrates an association between C. difficile fecal load and the results of routinely used diagnostic tests.
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correlation between clostridium difficile bacterial load commercial real time pcr cycle thresholds and results of diagnostic tests based on enzyme immunoAssay and cell culture Cytotoxicity Assay
Journal of Clinical Microbiology, 2013Co-Authors: Lealaurence Dionne, Frederic Raymond, Jacques Corbeil, Jean Longtin, Philippe Gervais, Yves LongtinAbstract:The impact of Clostridium difficile fecal loads on diagnostic test results is poorly understood, but it may have clinical importance. In this study, we investigated the relationship between C. difficile fecal load and the results of four Assays: a glutamate dehydrogenase (GDH) enzyme immunoAssay (EIA), a toxin A/B antigen EIA (ToxAB), a cell culture Cytotoxicity Assay (CCA), and PCR targeting the tcdB gene. We also compared the PCR cycle threshold (CT) with the results of quantitative culture using Spearman's rank correlation coefficient. Finally, we sequenced the genomes of 24 strains with different detection profiles. A total of 203 clinical samples harboring toxigenic C. difficile were analyzed and sorted into one of four groups: 17 PCR+ (group 1), 37 PCR+ GDH+ (group 2), 24 PCR+ GDH+ CCA+ (group 3), and 125 PCR+ GDH+ ToxAB+ (group 4). The overall median fecal load in log10 CFU/g was 6.67 (interquartile range [IQR], 5.57 to 7.54). The median fecal bacterial load of groups 1, 2, 3, and 4 were 4.15 (IQR, 3.00 to 4.98), 5.74 (IQR, 4.75 to 6.16), 6.20 (IQR, 5.23 to 6.80), and 7.08 (IQR, 6.35 to 7.83), respectively. Group 1 samples had lower fecal loads than those from each of the other groups (P < 0.001). Group 2 samples had lower fecal loads than those from groups 3 and 4 (P < 0.001). There was a significant correlation between PCR CT and fecal loads (ρ = −0.697; P < 0.001). NAP1 strains were associated with the detection of toxins by EIA or CCA (P = 0.041). This study demonstrates an association between C. difficile fecal load and the results of routinely used diagnostic tests.
