The Experts below are selected from a list of 291 Experts worldwide ranked by ideXlab platform
Jing Li - One of the best experts on this subject based on the ideXlab platform.
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a comparative study of cellular uptake and Cytotoxicity of multi walled carbon nanotubes graphene oxide and nanodiamond
Toxicology Research, 2012Co-Authors: Xiaoyong Zhang, Wenbing Hu, Jing LiAbstract:Investigations of the interactions between carbon nanomaterials (CNMs) and living organisms and their subsequent biological responses are of fundamental significance for toxicity assessment and further biomedical applications. In this work, the cellular uptake and Cytotoxicity of multi-walled carbon nanotubes (MWCNTs), graphene oxide (GO) and nanodiamond (ND) were examined and compared. We demonstrated that all of the CNMs were readily internalized by HeLa cells through nonspecific cellular uptake. Their cell uptake ratios showed significant differences in the following order: ND > MWCNTs > GO. A series of biological assays were used to evaluate the Cytotoxicity of CNMs. It was found that CNMs showed dose- and time-dependent Cytotoxicity to HeLa cells. However, Cytotoxicity of CNMs was not associated with their cell uptake ratios. Among them, ND exhibited the highest cell uptake ratio and the least Cytotoxicity. To the best of our knowledge, this is the first study which has quantitatively determined and compared the cell uptake ratios and cytotoxicities of MWCNTs, GO and ND. And we expect that these results described here could provide useful information for the development of new strategies to design efficient drug delivery nanocarriers and therapeutic systems as well as deep insights into the mechanism of CNMs’ Cytotoxicity.
Yuliang Zhao - One of the best experts on this subject based on the ideXlab platform.
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Cytotoxicity of carbon nanomaterials: single-wall nanotube, multi-wall nanotube, and fullerene.
Environmental Science & Technology, 2005Co-Authors: Haifang Wang, Xiang Wang, Yuliang ZhaoAbstract:A Cytotoxicity test protocol for single-wall nanotubes (SWNTs), multi-wall nanotubes (with diameters ranging from 10 to 20 nm, MWNT10), and fullerene (C60) was tested. Profound Cytotoxicity of SWNTs was observed in alveolar macrophage (AM) after a 6-h exposure in vitro. The Cytotoxicity increases by as high as ∼35% when the dosage of SWNTs was increased by 11.30 μg/cm2. No significant toxicity was observed for C60 up to a dose of 226.00 μg/cm2. The Cytotoxicity apparently follows a sequence order on a mass basis: SWNTs > MWNT10 > quartz > C60. SWNTs significantly impaired phagocytosis of AM at the low dose of 0.38 μg/cm2, whereas MWNT10 and C60 induced injury only at the high dose of 3.06 μg/cm2. The macrophages exposed to SWNTs or MWNT10 of 3.06 μg/cm2 showed characteristic features of necrosis and degeneration. A sign of apoptotic cell death likely existed. Carbon nanomaterials with different geometric structures exhibit quite different Cytotoxicity and bioactivity in vitro, although they may not be ac...
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Cytotoxicity of carbon nanomaterials: Single-wall nanotube, multi-wall nanotube, and fullerene
Environmental Science and Technology, 2005Co-Authors: Guang Jia, Rongjuan Pei, Tao Yan, Lei Yan, Haifang Wang, Yuliang Zhao, Xiang Wang, Xinbiao GuoAbstract:A Cytotoxicity test protocol for single-wall nanotubes (SWNTs), multi-wall nanotubes (with diameters ranging from 10 to 20 nm, MWNT10), and fullerene (C60) was tested. Profound Cytotoxicity of SWNTs was observed in alveolar macrophage (AM) after a 6-h exposure in vitro. The Cytotoxicity increases by as high as ∼35% when the dosage of SWNTs was increased by 11.30 μg/cm2. No significant toxicity was observed for C60 up to a dose of 226.00 μg/cm2. The Cytotoxicity apparently follows a sequence order on a mass basis: SWNTs > MWNT10 > quartz > C60. SWNTs significantly impaired phagocytosis of AM at the low dose of 0.38 μg/cm2, whereas MWNT10 and C60 induced injury only at the high dose of 3.06 μg/cm2. The macrophages exposed to SWNTs or MWNT10 of 3.06 μg/cm2 showed characteristic features of necrosis and degeneration. A sign of apoptotic cell death likely existed. Carbon nanomaterials with different geometric structures exhibit quite different Cytotoxicity and bioactivity in vitro, although they may not be accurately reflected in the comparative toxicity in vivo.
Xiaoyong Zhang - One of the best experts on this subject based on the ideXlab platform.
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a comparative study of cellular uptake and Cytotoxicity of multi walled carbon nanotubes graphene oxide and nanodiamond
Toxicology Research, 2012Co-Authors: Xiaoyong Zhang, Wenbing Hu, Jing LiAbstract:Investigations of the interactions between carbon nanomaterials (CNMs) and living organisms and their subsequent biological responses are of fundamental significance for toxicity assessment and further biomedical applications. In this work, the cellular uptake and Cytotoxicity of multi-walled carbon nanotubes (MWCNTs), graphene oxide (GO) and nanodiamond (ND) were examined and compared. We demonstrated that all of the CNMs were readily internalized by HeLa cells through nonspecific cellular uptake. Their cell uptake ratios showed significant differences in the following order: ND > MWCNTs > GO. A series of biological assays were used to evaluate the Cytotoxicity of CNMs. It was found that CNMs showed dose- and time-dependent Cytotoxicity to HeLa cells. However, Cytotoxicity of CNMs was not associated with their cell uptake ratios. Among them, ND exhibited the highest cell uptake ratio and the least Cytotoxicity. To the best of our knowledge, this is the first study which has quantitatively determined and compared the cell uptake ratios and cytotoxicities of MWCNTs, GO and ND. And we expect that these results described here could provide useful information for the development of new strategies to design efficient drug delivery nanocarriers and therapeutic systems as well as deep insights into the mechanism of CNMs’ Cytotoxicity.
R Schnettler - One of the best experts on this subject based on the ideXlab platform.
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an in vitro assessment of the antibacterial properties and Cytotoxicity of nanoparticulate silver bone cement
Biomaterials, 2004Co-Authors: Volker Alt, Thorsten Bechert, Peter Steinrucke, Michael Wagener, P Seidel, E Dingeldein, Eugen Domann, R SchnettlerAbstract:Abstract Infections with multiresistant bacteria have become a serious problem in joint arthroplasty. This study reports about in vitro antibacterial activity against multiresistant bacteria and in vitro Cytotoxicity of polymethylmetacrylate bone cement loaded with metallic silver particles with a size of 5–50 nm called NanoSilver. In vitro antibacterial activity against S. epidermidis , methicillin-resistant S. epidermidis (MRSE), and methicillin-resistant S. aureus (MRSA) was studied by microplate proliferation tests. Quantitative elution testing and qualitative ongrowth of human osteoblasts was done to study in vitro Cytotoxicity. Only NanoSilver cement showed high-antibacterial activity against all strains, including MRSE and MRSA. Gentamicin cement was not effective against MRSA and MRSE due to the high-level gentamicin resistance of the tested strains. Plain cement did not inhibit proliferation of any strains. There was no significant difference regarding in vitro cytotoxicty between NanoSilver and the non-toxic control. Cytotoxicity of cement loaded with silver salts made this kind of silver unsuitable for all day clinical use in the past. This new form of silver called NanoSilver was free of in vitro Cytotoxicity and showed high effectiveness against multiresistant bacteria. If the results can be confirmed in vivo NanoSilver may have a high interest in joint arthroplasty.
Haifang Wang - One of the best experts on this subject based on the ideXlab platform.
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Cytotoxicity of carbon nanomaterials: single-wall nanotube, multi-wall nanotube, and fullerene.
Environmental Science & Technology, 2005Co-Authors: Haifang Wang, Xiang Wang, Yuliang ZhaoAbstract:A Cytotoxicity test protocol for single-wall nanotubes (SWNTs), multi-wall nanotubes (with diameters ranging from 10 to 20 nm, MWNT10), and fullerene (C60) was tested. Profound Cytotoxicity of SWNTs was observed in alveolar macrophage (AM) after a 6-h exposure in vitro. The Cytotoxicity increases by as high as ∼35% when the dosage of SWNTs was increased by 11.30 μg/cm2. No significant toxicity was observed for C60 up to a dose of 226.00 μg/cm2. The Cytotoxicity apparently follows a sequence order on a mass basis: SWNTs > MWNT10 > quartz > C60. SWNTs significantly impaired phagocytosis of AM at the low dose of 0.38 μg/cm2, whereas MWNT10 and C60 induced injury only at the high dose of 3.06 μg/cm2. The macrophages exposed to SWNTs or MWNT10 of 3.06 μg/cm2 showed characteristic features of necrosis and degeneration. A sign of apoptotic cell death likely existed. Carbon nanomaterials with different geometric structures exhibit quite different Cytotoxicity and bioactivity in vitro, although they may not be ac...
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Cytotoxicity of carbon nanomaterials: Single-wall nanotube, multi-wall nanotube, and fullerene
Environmental Science and Technology, 2005Co-Authors: Guang Jia, Rongjuan Pei, Tao Yan, Lei Yan, Haifang Wang, Yuliang Zhao, Xiang Wang, Xinbiao GuoAbstract:A Cytotoxicity test protocol for single-wall nanotubes (SWNTs), multi-wall nanotubes (with diameters ranging from 10 to 20 nm, MWNT10), and fullerene (C60) was tested. Profound Cytotoxicity of SWNTs was observed in alveolar macrophage (AM) after a 6-h exposure in vitro. The Cytotoxicity increases by as high as ∼35% when the dosage of SWNTs was increased by 11.30 μg/cm2. No significant toxicity was observed for C60 up to a dose of 226.00 μg/cm2. The Cytotoxicity apparently follows a sequence order on a mass basis: SWNTs > MWNT10 > quartz > C60. SWNTs significantly impaired phagocytosis of AM at the low dose of 0.38 μg/cm2, whereas MWNT10 and C60 induced injury only at the high dose of 3.06 μg/cm2. The macrophages exposed to SWNTs or MWNT10 of 3.06 μg/cm2 showed characteristic features of necrosis and degeneration. A sign of apoptotic cell death likely existed. Carbon nanomaterials with different geometric structures exhibit quite different Cytotoxicity and bioactivity in vitro, although they may not be accurately reflected in the comparative toxicity in vivo.
