The Experts below are selected from a list of 32331 Experts worldwide ranked by ideXlab platform
Christopher W. Theodorakis - One of the best experts on this subject based on the ideXlab platform.
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Joint toxicity of sodium arsenate and sodium perchlorate to zebrafish Danio Rerio larvae.
Environmental toxicology and chemistry, 2005Co-Authors: Fujun Liu, Ronald J. Kendall, Christopher W. TheodorakisAbstract:Joint toxicity of arsenate and perchlorate was tested in larvae of Danio Rerio. Results indicated that the 96-h median lethal concentrations of sodium arsenate and sodium perchlorate were 258.8 and 1,401.2 mg/L, respectively, and that arsenate and perchlorate generally showed a concentration-additive effect.
Demian R Ifa - One of the best experts on this subject based on the ideXlab platform.
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Imaging of whole zebra fish (Danio Rerio) by desorption electrospray ionization mass spectrometry.
Rapid Communications in Mass Spectrometry, 2014Co-Authors: Alexander Chramow, Tanam S Hamid, Livia S Eberlin, Marion Girod, Demian R IfaAbstract:RATIONALE: To demonstrate the potential use of zebra fish (Danio Rerio) as a model vertebrate organism by producing two-dimensional ion images of the whole zebra fish, and being able to distinguish particular areas of interest such as the brain, spinal cord, and stomach region using a desorption electrospray ionization (DESI) ion source coupled to a linear ion trap. METHODS: Imaging experiments are performed on 45 µm sagittal slices of zebra fish (Danio Rerio), which are thaw-mounted onto microscope glass slides. The slides are then analyzed using a solvent of acetonitrile/dimethylformamide (50:50) (ACN/DMF), with a solvent flow rate of 1.5 μL/min; data are acquired in negative ion mode. Raw mass spectrum data files are converted into a readable file for Biomap. The images produced are then analyzed for ion distributions. RESULTS: We are able to create clear, distinct, chemical intensity images of the brain, spinal cord, and stomach based on lipid content as well as bile salt. The identities of these compounds were confirmed by tandem mass spectrometric (MS/MS) experiments and comparisons with literature. CONCLUSIONS: Imaging of whole zebra fish is possible using ambient ionization techniques such as DESI. Analyses are fast and reliable. For most of the compounds observed, the identification by MS/MS can be performed directly from the fish tissue sample. Copyright © 2014 John Wiley & Sons, Ltd.
Steve F Perry - One of the best experts on this subject based on the ideXlab platform.
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The tight junction protein claudin-b regulates epithelial permeability and sodium handling in larval zebrafish, Danio Rerio
American Journal of Physiology-regulatory Integrative and Comparative Physiology, 2013Co-Authors: Raymond W. M. Kwong, Steve F PerryAbstract:The functional role of the tight junction protein claudin-b in larval zebrafish (Danio Rerio) was investigated. We showed that claudin-b protein is expressed at epithelial cell-cell contacts on the...
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β-Adrenergic regulation of Na+ uptake by larval zebrafish Danio Rerio in acidic and ion-poor environments
American journal of physiology. Regulatory integrative and comparative physiology, 2012Co-Authors: Yusuke Kumai, Mellissa A. R. Ward, Steve F PerryAbstract:The potential role of adrenergic systems in regulating Na+ uptake in zebrafish (Danio Rerio) larvae was investigated. Treatment with isoproterenol (a generic β-adrenergic receptor agonist) stimulat...
Inger Porsch Hällström - One of the best experts on this subject based on the ideXlab platform.
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17α-Ethinyl estradiol affects anxiety and shoaling behavior in adult male zebra fish (Danio Rerio).
Aquatic Toxicology, 2011Co-Authors: Nasim Reyhanian, Kristina Volkova, Stefan Hallgren, Tomas Bollner, Per-erik Olsson, K. Håkan Olsén, Inger Porsch HällströmAbstract:17α-Ethinyl estradiol affects anxiety and shoaling behavior in adult male zebra fish (Danio Rerio)
Zdenka Svobodova - One of the best experts on this subject based on the ideXlab platform.
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assessment of diclofenac lc50 reference values in juvenile and embryonic stages of the zebrafish Danio Rerio
Polish Journal of Veterinary Sciences, 2011Co-Authors: Eva Praskova, Stanislava Macova, Eva Voslarova, Lucie Plhalova, Petr Marsalek, Zuzana Siroka, Vladimira Pistekova, Zdenka SvobodovaAbstract:The aim of the study was to compare the acute toxicity of diclofenac to juvenile and embryonic stages of the zebrafish (Danio Rerio). Acute toxicity tests were performed on the aquarium fish Danio Rerio, which is one of the model organisms most commonly used in toxicity testing. The tests were performed using a semi-static method according to OECD guideline No. 203 (Fish, acute toxicity test). Embryo toxicity tests were performed in zebrafish embryos (Danio Rerio) in compliance with OECD No. 212 methodology (Fish, short-term toxicity test on embryo and sac-fry stages). The results were subjected to a probit analysis using the EKO-TOX 5.2 programme to determine 96hLC50 and 144hLC50 (median lethal concentration, 50% mortality after a 96 h or 144 h interval, respectively) values of diclofenac. The statistical significance of the difference between LC50 values in juvenile and embryonic stages of Danio Rerio was tested using the Mann-Whitney non-parametric test implemented in the Unistat 5.1 programme. The LC50 mean value of diclofenac was 166.6 +/- 9.8 mg/L in juvenile Danio Rerio, and 6.11 +/- 2.48 mg/L in embryonic stages of Danio Rerio. The study demonstrated a statistically higher sensitivity to diclofenac (P < 0.05) in embryonic stages compared to the juvenile fish.
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terbutryn toxicity to Danio Rerio effects of subchronic exposure on fish growth
Neuro endocrinology letters, 2009Co-Authors: Lucie Plhalova, Stanislava Macova, Ivana Haluzova, Andrea Slaninova, Eva Voslarova, Vladimira Pistěkova, Petra Doleželova, Petr Marsalek, Iveta Bedanova, Zdenka SvobodovaAbstract:OBJECTIVES: The aim of this study was to investigate effects of subchronic exposure to sublethal levels of terbutryn on growth and on histopathological changes in Danio Rerio. DESIGN: The acute toxicity tests were performed on the juvenile stage of Danio Rerio according to OECD No. 203 Fish, Acute Toxicity Test. The juvenile growth tests were performed on D. Rerio according to the OECD method No. 215. Fish at the age of 20 days were exposed to the terbutryn environmental concentration commonly detected in the Czech rivers (0.02 microg L-1) and the range of sublethal concentrations of terbutryn (0.06, 0.2, 0.6 and 2 mg L-1) for 28 days. RESULTS: The 96hLC50 terbutryn mean value for the juvenile stage of D. Rerio was 5.71 +/- 0.46 mg L-1. A significant decrease (p < 0.01) in the fish growth caused by terbutryn was observed in the concentration of 0.6 mg L-1. The value of NOEC was 0.2 mg L-1 and LOEC was 0.6 mg L-1 of terbutryn. We found the damage to tubular system of kidneys in the concentration of 0.6 mg L-1 of terbutryn. CONCLUSION: The environmental concentration of terbutryn in the Czech rivers did not have any effects on growth and on histopathological changes in D. Rerio and this concentration is lower than determined LOEC and NOEC values of terbutryn.
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Terbutryn toxicity to Danio Rerio: effects of subchronic exposure on fish growth.
Neuro endocrinology letters, 2009Co-Authors: Lucie Plhalova, Stanislava Macova, Ivana Haluzova, Andrea Slaninova, Eva Voslarova, Petra Doleželova, Petr Marsalek, Iveta Bedanova, Vladimira Pistekova, Zdenka SvobodovaAbstract:The aim of this study was to investigate effects of subchronic exposure to sublethal levels of terbutryn on growth and on histopathological changes in Danio Rerio. The acute toxicity tests were performed on the juvenile stage of Danio Rerio according to OECD No. 203 Fish, Acute Toxicity Test. The juvenile growth tests were performed on D. Rerio according to the OECD method No. 215. Fish at the age of 20 days were exposed to the terbutryn environmental concentration commonly detected in the Czech rivers (0.02 microg L-1) and the range of sublethal concentrations of terbutryn (0.06, 0.2, 0.6 and 2 mg L-1) for 28 days. The 96hLC50 terbutryn mean value for the juvenile stage of D. Rerio was 5.71 +/- 0.46 mg L-1. A significant decrease (p < 0.01) in the fish growth caused by terbutryn was observed in the concentration of 0.6 mg L-1. The value of NOEC was 0.2 mg L-1 and LOEC was 0.6 mg L-1 of terbutryn. We found the damage to tubular system of kidneys in the concentration of 0.6 mg L-1 of terbutryn. The environmental concentration of terbutryn in the Czech rivers did not have any effects on growth and on histopathological changes in D. Rerio and this concentration is lower than determined LOEC and NOEC values of terbutryn.
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Nitrite Toxicity to Danio Rerio : Effects of Subchronic Exposure on Fish Growth
Acta Veterinaria Brno, 2008Co-Authors: Eva Voslářová, Vladimira Pistěkova, Zdenka Svobodova, Iveta BedáňováAbstract:Voslařova E., V. Pistěkova, Z. Svobodova, I. Bedaňova: Nitrite Toxicity to Danio Rerio: Effects of Subchronic Exposure on Fish Growth. Acta Vet. Brno 2008, 77: 455-460. The aim of this study was to investigate the long-term effects of subchronic exposure to sublethal levels of nitrite, ranging from 15 to 130 mg·l -1 NO2 , on growth in aquarium fish Danio Rerio. The juvenile growth test according to OECD 215 was used in the experiments. Fish weight was measured at the beginning of the experiment and then using the same method, fish weight was observed 28 days after fish stocking. Compared to the control, growth suppression was detected from the concentration of 73 mg·l -1 NO2 (P < 0.05) and a significant inhibition of fish body growth was shown from 130 mg·l -1 NO 2 (P < 0.01). An exponential relationship between nitrite concentrations and specific growth rate (R 2 = 0.896) was detected.
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Evaluation of xenoestrogenic potential of propylparaben in zebrafish (Danio Rerio).
Neuro endocrinology letters, 2006Co-Authors: Premysl Mikula, Zdenka Svobodova, Radka Dobsikova, Jirí JarkovskýAbstract:Xenoestrogenic potential of propylparaben (PP), one of the most commonly used preservatives in drugs, cosmetics and food, was investigated in vivo using zebrafish (Danio Rerio). Juvenile zebrafish (20 days post hatching) were exposed to three different concentrations of propylparaben (PP) dissolved in ethanol and added into the water. After 20 days of exposure the fish were euthanized and vitellogenin concentrations in their whole body homogenates were measured by the method of direct sandwich ELISA. Simultaneously, vitellogenin concentrations in either fish from the control group (exposed to solvent without the substance tested) and in fish from the positive control group (exposed to natural estrogen 17beta-estradiol) were measured. Vitellogenin concentration in whole body homogenates of control fish was 400 (396-540) ng/ml(-1) (geometric mean (95% CI)). Zebrafish exposure to propylparaben at the concentrations of 0.1; 0.4 and 0.9 mg/l(-1) elicited statistically significant decline (P<0.001) of vitellogenin production, i.e. geometric means of vitellogenin concentrations in whole body homogenates were 240 (186-311); 218 (175-270) and 270 (234-311) ng/ml(-1), respectively. Conversely, the geometric mean of vitellogenin concentration in whole body homogenates of zebrafish exposed to 100 ng/ml(-1) of 17beta-estradiol (positive control) was significantly higher (P<0.001) than values in all other groups, i.e. 35,553 (16,860-74,968) ng/ml(-1). Our results suggest an antiestrogenic potential of propylparaben tested in vivo in juvenile zebrafish (Danio Rerio). The estrogenic effect of 17beta-estradiol was confirmed.
