The Experts below are selected from a list of 56319 Experts worldwide ranked by ideXlab platform
Fei Sun - One of the best experts on this subject based on the ideXlab platform.
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reprint of amorphous nickel titanium alloy film a new choice for cryo electron microscopy sample preparation
Progress in Biophysics & Molecular Biology, 2021Co-Authors: Xiaojun Huang, Lei Zhang, Zuoling Wen, Hui Chen, Changcheng Yin, Fei SunAbstract:Abstract Cryo-electron microscopy (cryoEM) has become one of the most important approach for structural biology. However, barriers are still there for an increased successful rate, a better resolution and improved efficiency from sample preparation, Data Collection to image processing. CryoEM sample preparation is one of the bottlenecks with many efforts made recently, including the optimization of supporting substrate (e.g. ultra-thin carbon, graphene, pure gold, 2d crystal of streptavidin, and affinity modification), which was aimed to solve air-water interface problem, or reduce beam induced motion (BIM), or change particle distribution in the grid hole. Here, we report another effort of developing a new supporting substrate, the amorphous nickel-titanium alloy (ANTA) film, for cryoEM sample preparation as a layer of holey supporting film covering on TEM grid. Our investigations showed advantages of ANTA film in comparison with conventional carbon film, including much better electron conductivity and trace non-specific interaction with protein. These advantages yield less BIM and significantly improved particle distribution during cryoEM experiment of human apo-ferritn, thus resulting an improved reconstruction resolution from a reduced number of micrographs and particles. Unlike the pure gold film, the usage of the ANTA film is just same with the carbon film, compatible to conventional automatic cryoEM Data Collection Procedure.
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amorphous nickel titanium alloy film a new choice for cryo electron microscopy sample preparation
Progress in Biophysics & Molecular Biology, 2020Co-Authors: Xiaojun Huang, Lei Zhang, Zuoling Wen, Hui Chen, Changcheng Yin, Fei SunAbstract:Cryo-electron microscopy (cryoEM) has become one of the most important approach for structural biology. However, barriers are still there for an increased successful rate, a better resolution and improved efficiency from sample preparation, Data Collection to image processing. CryoEM sample preparation is one of the bottlenecks with many efforts made recently, including the optimization of supporting substrate (e.g. ultra-thin carbon, graphene, pure gold, 2d crystal of streptavidin, and affinity modification), which was aimed to solve air-water interface problem, or reduce beam induced motion (BIM), or change particle distribution in the grid hole. Here, we report another effort of developing a new supporting substrate, the amorphous nickel-titanium alloy (ANTA) film, for cryoEM sample preparation as a layer of holey supporting film covering on TEM grid. Our investigations showed advantages of ANTA film in comparison with conventional carbon film, including much better electron conductivity and trace non-specific interaction with protein. These advantages yield less BIM and significantly improved particle distribution during cryoEM experiment of human apo-ferritn, thus resulting an improved reconstruction resolution from a reduced number of micrographs and particles. Unlike the pure gold film, the usage of the ANTA film is just same with the carbon film, compatible to conventional automatic cryoEM Data Collection Procedure.
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amorphous nickel titanium alloy film a new choice for cryo electron microscopy sample preparation
bioRxiv, 2020Co-Authors: Xiaojun Huang, Lei Zhang, Zuoling Wen, Hui Chen, Changcheng Yin, Fei SunAbstract:Cryo-electron microscopy (cryoEM) has become one of the most important approach for structural biology. However, barriers are still there for an increase successful rate, a better resolution and improved efficiency from sample preparation, Data Collection to image processing. CryoEM sample preparation is one of the bottlenecks with many efforts made recently, including the optimization of supporting substrate (e.g. ultra-thin carbon, graphene, pure gold, 2d crystal of streptavidin, and affinity modification), which was aimed to solve air-water interface problem, or reduce beam induced motion (BIM), or change particle distribution in the grid hole. Here, we report another effort of developing a new supporting substrate, the amorphous nickel-titanium alloy (ANTA) film, for cryoEM sample preparation. Our investigations showed advantages of ANTA film in comparison with conventional carbon film, including superior electron conductivity and trace non-specific interaction with protein. These advantages yield less BIM and significantly improved particle distribution during cryoEM experiment of human apo-ferritn, thus resulting an improved reconstruction resolution from a reduced number of micrographs and particles. Unlike the pure gold film, the usage of the ANTA film is just same with the carbon film, compatible to conventional automatic cryoEM Data Collection Procedure.
Yuval Neria - One of the best experts on this subject based on the ideXlab platform.
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when home isn t a safe haven insecure attachment orientations perceived social support and ptsd symptoms among israeli evacuees under missile threat
Psychological Trauma: Theory Research Practice and Policy, 2012Co-Authors: Avi Besser, Yuval NeriaAbstract:This study examined a theoretically based mediation model including intensity of trauma exposure, adult attachment, perceived social support, and posttraumatic stress disorder (PTSD) symptoms. The sample consisted of 135 Israeli students who were evacuated from a college campus located near the Israel–Gaza border in response to increased missile fire in the area. An Internet-based Data Collection Procedure was used to survey the evacuees, who remained within the affected region. Individuals at different locations within the affected area were exposed to different levels of trauma intensity. Results indicated that intensity of trauma exposure did not affect levels of PTSD symptoms or perceived social support. Structural equation modeling showed that the link between attachment anxiety and PTSD symptoms is mediated by low levels of perceived social support. These findings underscore the role of interpersonal resources in the psychological sequelae of exposure to a continuous, life-threatening situation by highlighting the negative role played by low perceived social support among insecurely attached individuals exposed to missile attacks. Theoretical and clinical implications of the findings are discussed.
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the effects of insecure attachment orientations and perceived social support on posttraumatic stress and depressive symptoms among civilians exposed to the 2009 israel gaza war a follow up cross lagged panel design study
Journal of Research in Personality, 2010Co-Authors: Avi Besser, Yuval NeriaAbstract:A follow-up Cross-Lagged-design was used to test the effects of attachment orientations and perceived social support on posttraumatic stress disorder (PTSD) and major depressive disorder symptoms (MDD) in a sample of 135 Israeli students who were evacuated from a university campus located near the Israel–Gaza border in response to increased missile-fire in the area. An internet-based Data Collection Procedure enabled the simultaneous survey of evacuees located up to 40 km from the border at war, both during the fighting and 4 months after the ceasefire. Proximity to the border did not affect levels of PTSD or MDD symptoms, attachment orientation, or levels of perceived social support. Analyses involving Cross-Lagged Panel Correlation (CLPC) path models revealed that Attachment–Anxiety had significant positive effects on PTSD, MDD, and perceived social support. Neither PTSD nor MDD nor perceived social support had any reciprocal follow-up effect on Attachment–Anxiety. These findings underscore the central role of individual trait personality differences in predicting changes in both mental health problems and interpersonal relations over time, following exposure to trauma.
Xiaojun Huang - One of the best experts on this subject based on the ideXlab platform.
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reprint of amorphous nickel titanium alloy film a new choice for cryo electron microscopy sample preparation
Progress in Biophysics & Molecular Biology, 2021Co-Authors: Xiaojun Huang, Lei Zhang, Zuoling Wen, Hui Chen, Changcheng Yin, Fei SunAbstract:Abstract Cryo-electron microscopy (cryoEM) has become one of the most important approach for structural biology. However, barriers are still there for an increased successful rate, a better resolution and improved efficiency from sample preparation, Data Collection to image processing. CryoEM sample preparation is one of the bottlenecks with many efforts made recently, including the optimization of supporting substrate (e.g. ultra-thin carbon, graphene, pure gold, 2d crystal of streptavidin, and affinity modification), which was aimed to solve air-water interface problem, or reduce beam induced motion (BIM), or change particle distribution in the grid hole. Here, we report another effort of developing a new supporting substrate, the amorphous nickel-titanium alloy (ANTA) film, for cryoEM sample preparation as a layer of holey supporting film covering on TEM grid. Our investigations showed advantages of ANTA film in comparison with conventional carbon film, including much better electron conductivity and trace non-specific interaction with protein. These advantages yield less BIM and significantly improved particle distribution during cryoEM experiment of human apo-ferritn, thus resulting an improved reconstruction resolution from a reduced number of micrographs and particles. Unlike the pure gold film, the usage of the ANTA film is just same with the carbon film, compatible to conventional automatic cryoEM Data Collection Procedure.
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amorphous nickel titanium alloy film a new choice for cryo electron microscopy sample preparation
Progress in Biophysics & Molecular Biology, 2020Co-Authors: Xiaojun Huang, Lei Zhang, Zuoling Wen, Hui Chen, Changcheng Yin, Fei SunAbstract:Cryo-electron microscopy (cryoEM) has become one of the most important approach for structural biology. However, barriers are still there for an increased successful rate, a better resolution and improved efficiency from sample preparation, Data Collection to image processing. CryoEM sample preparation is one of the bottlenecks with many efforts made recently, including the optimization of supporting substrate (e.g. ultra-thin carbon, graphene, pure gold, 2d crystal of streptavidin, and affinity modification), which was aimed to solve air-water interface problem, or reduce beam induced motion (BIM), or change particle distribution in the grid hole. Here, we report another effort of developing a new supporting substrate, the amorphous nickel-titanium alloy (ANTA) film, for cryoEM sample preparation as a layer of holey supporting film covering on TEM grid. Our investigations showed advantages of ANTA film in comparison with conventional carbon film, including much better electron conductivity and trace non-specific interaction with protein. These advantages yield less BIM and significantly improved particle distribution during cryoEM experiment of human apo-ferritn, thus resulting an improved reconstruction resolution from a reduced number of micrographs and particles. Unlike the pure gold film, the usage of the ANTA film is just same with the carbon film, compatible to conventional automatic cryoEM Data Collection Procedure.
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amorphous nickel titanium alloy film a new choice for cryo electron microscopy sample preparation
bioRxiv, 2020Co-Authors: Xiaojun Huang, Lei Zhang, Zuoling Wen, Hui Chen, Changcheng Yin, Fei SunAbstract:Cryo-electron microscopy (cryoEM) has become one of the most important approach for structural biology. However, barriers are still there for an increase successful rate, a better resolution and improved efficiency from sample preparation, Data Collection to image processing. CryoEM sample preparation is one of the bottlenecks with many efforts made recently, including the optimization of supporting substrate (e.g. ultra-thin carbon, graphene, pure gold, 2d crystal of streptavidin, and affinity modification), which was aimed to solve air-water interface problem, or reduce beam induced motion (BIM), or change particle distribution in the grid hole. Here, we report another effort of developing a new supporting substrate, the amorphous nickel-titanium alloy (ANTA) film, for cryoEM sample preparation. Our investigations showed advantages of ANTA film in comparison with conventional carbon film, including superior electron conductivity and trace non-specific interaction with protein. These advantages yield less BIM and significantly improved particle distribution during cryoEM experiment of human apo-ferritn, thus resulting an improved reconstruction resolution from a reduced number of micrographs and particles. Unlike the pure gold film, the usage of the ANTA film is just same with the carbon film, compatible to conventional automatic cryoEM Data Collection Procedure.
Milica Gasic - One of the best experts on this subject based on the ideXlab platform.
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multiwoz a large scale multi domain wizard of oz Dataset for task oriented dialogue modelling
arXiv: Computation and Language, 2018Co-Authors: Pawel Budzianowski, Tsunghsien Wen, Bohsiang Tseng, Inigo Casanueva, Stefan Ultes, Osman Ramadan, Milica GasicAbstract:Even though machine learning has become the major scene in dialogue research community, the real breakthrough has been blocked by the scale of Data available. To address this fundamental obstacle, we introduce the Multi-Domain Wizard-of-Oz Dataset (MultiWOZ), a fully-labeled Collection of human-human written conversations spanning over multiple domains and topics. At a size of $10$k dialogues, it is at least one order of magnitude larger than all previous annotated task-oriented corpora. The contribution of this work apart from the open-sourced Dataset labelled with dialogue belief states and dialogue actions is two-fold: firstly, a detailed description of the Data Collection Procedure along with a summary of Data structure and analysis is provided. The proposed Data-Collection pipeline is entirely based on crowd-sourcing without the need of hiring professional annotators; secondly, a set of benchmark results of belief tracking, dialogue act and response generation is reported, which shows the usability of the Data and sets a baseline for future studies.
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multiwoz a large scale multi domain wizard of oz Dataset for task oriented dialogue modelling
Empirical Methods in Natural Language Processing, 2018Co-Authors: Pawel Budzianowski, Tsunghsien Wen, Bohsiang Tseng, Inigo Casanueva, Stefan Ultes, Osman Ramadan, Milica GasicAbstract:Even though machine learning has become the major scene in dialogue research community, the real breakthrough has been blocked by the scale of Data available.To address this fundamental obstacle, we introduce the Multi-Domain Wizard-of-Oz Dataset (MultiWOZ), a fully-labeled Collection of human-human written conversations spanning over multiple domains and topics.At a size of 10k dialogues, it is at least one order of magnitude larger than all previous annotated task-oriented corpora.The contribution of this work apart from the open-sourced Dataset is two-fold:firstly, a detailed description of the Data Collection Procedure along with a summary of Data structure and analysis is provided. The proposed Data-Collection pipeline is entirely based on crowd-sourcing without the need of hiring professional annotators;secondly, a set of benchmark results of belief tracking, dialogue act and response generation is reported, which shows the usability of the Data and sets a baseline for future studies.
Avi Besser - One of the best experts on this subject based on the ideXlab platform.
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when home isn t a safe haven insecure attachment orientations perceived social support and ptsd symptoms among israeli evacuees under missile threat
Psychological Trauma: Theory Research Practice and Policy, 2012Co-Authors: Avi Besser, Yuval NeriaAbstract:This study examined a theoretically based mediation model including intensity of trauma exposure, adult attachment, perceived social support, and posttraumatic stress disorder (PTSD) symptoms. The sample consisted of 135 Israeli students who were evacuated from a college campus located near the Israel–Gaza border in response to increased missile fire in the area. An Internet-based Data Collection Procedure was used to survey the evacuees, who remained within the affected region. Individuals at different locations within the affected area were exposed to different levels of trauma intensity. Results indicated that intensity of trauma exposure did not affect levels of PTSD symptoms or perceived social support. Structural equation modeling showed that the link between attachment anxiety and PTSD symptoms is mediated by low levels of perceived social support. These findings underscore the role of interpersonal resources in the psychological sequelae of exposure to a continuous, life-threatening situation by highlighting the negative role played by low perceived social support among insecurely attached individuals exposed to missile attacks. Theoretical and clinical implications of the findings are discussed.
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the effects of insecure attachment orientations and perceived social support on posttraumatic stress and depressive symptoms among civilians exposed to the 2009 israel gaza war a follow up cross lagged panel design study
Journal of Research in Personality, 2010Co-Authors: Avi Besser, Yuval NeriaAbstract:A follow-up Cross-Lagged-design was used to test the effects of attachment orientations and perceived social support on posttraumatic stress disorder (PTSD) and major depressive disorder symptoms (MDD) in a sample of 135 Israeli students who were evacuated from a university campus located near the Israel–Gaza border in response to increased missile-fire in the area. An internet-based Data Collection Procedure enabled the simultaneous survey of evacuees located up to 40 km from the border at war, both during the fighting and 4 months after the ceasefire. Proximity to the border did not affect levels of PTSD or MDD symptoms, attachment orientation, or levels of perceived social support. Analyses involving Cross-Lagged Panel Correlation (CLPC) path models revealed that Attachment–Anxiety had significant positive effects on PTSD, MDD, and perceived social support. Neither PTSD nor MDD nor perceived social support had any reciprocal follow-up effect on Attachment–Anxiety. These findings underscore the central role of individual trait personality differences in predicting changes in both mental health problems and interpersonal relations over time, following exposure to trauma.
