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Yasushi Sugano - One of the best experts on this subject based on the ideXlab platform.
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Degradation of the synthetic dye amaranth by the fungus bjerkandera adusta dec 1 inference of the Degradation Pathway from an analysis of decolorized products
Biodegradation, 2011Co-Authors: Nichina Gomi, Shuji Yoshida, Kazutsugu Matsumoto, Masayuki Okudomi, Hiroki Konno, Toru Hisabori, Yasushi SuganoAbstract:We examined the Degradation of amaranth, a representative azo dye, by Bjerkandera adusta Dec 1. The Degradation products were analyzed by high performance liquid chromatography (HPLC), visible absorbance, and electrospray ionization time-of-flight mass spectroscopy (ESI-TOF-MS). At the primary culture stage (3 days), the probable reaction intermediates were 1-aminonaphthalene-2,3,6-triol, 4-(hydroxyamino) naphthalene-1-ol, and 2-hydroxy-3-[2-(4-sulfophenyl) hydrazinyl] benzenesulfonic acid. After 10 days, the reaction products detected were 4-nitrophenol, phenol, 2-hydroxy-3-nitrobenzenesulfonic acid, 4-nitrobenzene sulfonic acid, and 3,4′-disulfonyl azo benzene, suggesting that no aromatic amines were created. Manganese-dependent peroxidase activity increased sharply after 3 days culture. Based on these results, we herein propose, for the first time, a Degradation Pathway for amaranth. Our results suggest that Dec 1 degrades amaranth via the combined activities of peroxidase and hydrolase and reductase action.
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Degradation of the synthetic dye amaranth by the fungus bjerkandera adusta dec 1 inference of the Degradation Pathway from an analysis of decolorized products
Biodegradation, 2011Co-Authors: Nichina Gomi, Shuji Yoshida, Kazutsugu Matsumoto, Masayuki Okudomi, Hiroki Konno, Toru Hisabori, Yasushi SuganoAbstract:We examined the Degradation of amaranth, a representative azo dye, by Bjerkandera adusta Dec 1. The Degradation products were analyzed by high performance liquid chromatography (HPLC), visible absorbance, and electrospray ionization time-of-flight mass spectroscopy (ESI-TOF-MS). At the primary culture stage (3 days), the probable reaction intermediates were 1-aminonaphthalene-2,3,6-triol, 4-(hydroxyamino) naphthalene-1-ol, and 2-hydroxy-3-[2-(4-sulfophenyl) hydrazinyl] benzenesulfonic acid. After 10 days, the reaction products detected were 4-nitrophenol, phenol, 2-hydroxy-3-nitrobenzenesulfonic acid, 4-nitrobenzene sulfonic acid, and 3,4′-disulfonyl azo benzene, suggesting that no aromatic amines were created. Manganese-dependent peroxidase activity increased sharply after 3 days culture. Based on these results, we herein propose, for the first time, a Degradation Pathway for amaranth. Our results suggest that Dec 1 degrades amaranth via the combined activities of peroxidase and hydrolase and reductase action.
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Degradation Pathway of an anthraquinone dye catalyzed by a unique peroxidase dyp from thanatephorus cucumeris dec 1
Biodegradation, 2009Co-Authors: Yasushi Sugano, Yuko Matsushima, Katsunori Tsuchiya, Hirokazu Aoki, Mitsuyo Hirai, Makoto ShodaAbstract:The reactants produced by action of a purified unique dye-decolorizing peroxidase, DyP, on a commercial anthraquinone dye, Reactive Blue 5, were investigated using electrospray ionization mass spectrometry (ESI-MS), thin-layer chromatography (TLC), and 1H- and 13C- nuclear magnetic resonance (NMR). The results of ESI-MS analysis showed that phthalic acid, a Product 2 (molecular weight 472.5), and a Product 3 (molecular weight 301.5), were produced. Product 2 and Product 3 were generated by usual peroxidase reaction, whereas phthalic acid was generated by hydrolase- or oxygenase-catalyzed reaction. One potential associated product, o-aminobenzene sulfonic acid, was found to be converted to 2,2′-disulfonyl azobenzene by ESI-MS and NMR analyses. From these results, we propose, for the first time, the Degradation Pathway of an anthraquinone dye by the enzyme DyP.
Jens Nielsen - One of the best experts on this subject based on the ideXlab platform.
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Improved production of fatty acid ethyl esters in Saccharomyces cerevisiae through up-regulation of the ethanol Degradation Pathway and expression of the heterologous phosphoketolase Pathway
Microbial cell factories, 2014Co-Authors: Bouke Wim De Jong, Shuobo Shi, Verena Siewers, Jens NielsenAbstract:Due to an increasing demand of transportation fuels, a lower availability of cheap crude oil and a lack of sustainability of fossil fuels, a gradual shift from petroleum based fuels towards alternative and renewable fuel resources will be required in the near future. Fatty acid ethyl esters (FAEEs) have properties similar to current crude diesel and could therefore form an important contribution to the development of sustainable transportation fuels in future. It is important to develop novel cell factories for efficient production of FAEEs and their precursors. Here, a Saccharomyces cerevisiae cell factory expressing a heterologous wax ester synthase (ws2) from Marinobacter hydrocarbonoclasticus was used to produce FAEEs from ethanol and acyl-coenzyme A (acyl-CoA). The production of acyl-CoA requires large amounts of NADPH and acetyl-CoA. Therefore, two metabolic engineering strategies for improved provision of NADPH and acetyl-CoA were evaluated. First, the ethanol Degradation Pathway was employed to re-channel carbon flow towards the synthesis of acetyl-CoA. Therefore, ADH2 and ALD6 encoding, respectively, alcohol dehydrogenase and acetaldehyde dehydrogenase were overexpressed together with the heterologous gene acs SE L641P encoding acetyl-CoA synthetase. The co-overexpression of ADH2, ALD6 and acs SE L641P with ws2 resulted in 408 ± 270 μg FAEE gCDW−1, a 3-fold improvement. Secondly, for the expression of the PHK Pathway two genes, xpkA and ack, both descending from Aspergillus nidulans, were co-expressed together with ws2 to catalyze, respectively, the conversion of xylulose-5-phosphate to acetyl phosphate and glyceraldehyde-3-phosphate and acetyl phosphate to acetate. Alternatively, ack was substituted with pta from Bacillus subtilis, encoding phosphotransacetylase for the conversion of acetyl phosphate to acetyl-CoA. Both PHK Pathways were additionally expressed in a strain with multiple chromosomally integrated ws2 gene, which resulted in respectively 5100 ± 509 and 4670 ± 379 μg FAEE gCDW−1, an up to 1.7-fold improvement. Two different strategies for engineering of the central carbon metabolism for efficient provision of acetyl-CoA and NADPH required for fatty acid biosynthesis and hence FAEE production were evaluated and it was found that both the ethanol Degradation Pathway as well as the phosphoketolase Pathway improve the yield of FAEEs.
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Improved production of fatty acid ethyl esters in Saccharomyces cerevisiae through up-regulation of the ethanol Degradation Pathway and expression of the heterologous phosphoketolase Pathway
Microbial Cell Factories, 2014Co-Authors: Bouke Wim De Jong, Shuobo Shi, Verena Siewers, Jens NielsenAbstract:BACKGROUND: Due to an increasing demand of transportation fuels, a lower availability of cheap crude oil and a lack of sustainability of fossil fuels, a gradual shift from petroleum based fuels towards alternative and renewable fuel resources will be required in the near future. Fatty acid ethyl esters (FAEEs) have properties similar to current crude diesel and could therefore form an important contribution to the development of sustainable transportation fuels in future. It is important to develop novel cell factories for efficient production of FAEEs and their precursors.\n\nRESULTS: Here, a Saccharomyces cerevisiae cell factory expressing a heterologous wax ester synthase (ws2) from Marinobacter hydrocarbonoclasticus was used to produce FAEEs from ethanol and acyl-coenzyme A (acyl-CoA). The production of acyl-CoA requires large amounts of NADPH and acetyl-CoA. Therefore, two metabolic engineering strategies for improved provision of NADPH and acetyl-CoA were evaluated. First, the ethanol Degradation Pathway was employed to re-channel carbon flow towards the synthesis of acetyl-CoA. Therefore, ADH2 and ALD6 encoding, respectively, alcohol dehydrogenase and acetaldehyde dehydrogenase were overexpressed together with the heterologous gene acsSEL641P encoding acetyl-CoA synthetase. The co-overexpression of ADH2, ALD6 and acsSEL641P with ws2 resulted in 408 ± 270 μg FAEE gCDW-1, a 3-fold improvement. Secondly, for the expression of the PHK Pathway two genes, xpkA and ack, both descending from Aspergillus nidulans, were co-expressed together with ws2 to catalyze, respectively, the conversion of xylulose-5-phosphate to acetyl phosphate and glyceraldehyde-3-phosphate and acetyl phosphate to acetate. Alternatively, ack was substituted with pta from Bacillus subtilis, encoding phosphotransacetylase for the conversion of acetyl phosphate to acetyl-CoA. Both PHK Pathways were additionally expressed in a strain with multiple chromosomally integrated ws2 gene, which resulted in respectively 5100 ± 509 and 4670 ± 379 μg FAEE gCDW-1, an up to 1.7-fold improvement.\n\nCONCLUSION: Two different strategies for engineering of the central carbon metabolism for efficient provision of acetyl-CoA and NADPH required for fatty acid biosynthesis and hence FAEE production were evaluated and it was found that both the ethanol Degradation Pathway as well as the phosphoketolase Pathway improve the yield of FAEEs.
Carl E Cerniglia - One of the best experts on this subject based on the ideXlab platform.
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complete and integrated pyrene Degradation Pathway in mycobacterium vanbaalenii pyr 1 based on systems biology
Journal of Bacteriology, 2007Co-Authors: Seongjae Kim, Ohgew Kweon, Richard C Jones, J P Freeman, Ricky D Edmondson, Carl E CernigliaAbstract:Mycobacterium vanbaalenii PYR-1 was the first bacterium isolated by virtue of its ability to metabolize the high-molecular-weight polycyclic aromatic hydrocarbon (PAH) pyrene. We used metabolic, genomic, and proteomic approaches in this investigation to construct a complete and integrated pyrene Degradation Pathway for M. vanbaalenii PYR-1. Genome sequence analyses identified genes involved in the pyrene Degradation Pathway that we have proposed for this bacterium. To identify proteins involved in the Degradation, we conducted a proteome analysis of cells exposed to pyrene using one-dimensional gel electrophoresis in combination with liquid chromatography-tandem mass spectrometry. Database searching performed with the M. vanbaalenii PYR-1 genome resulted in identification of 1,028 proteins with a protein false discovery rate of <1%. Based on both genomic and proteomic data, we identified 27 enzymes necessary for constructing a complete Pathway for pyrene Degradation. Our analyses indicate that this bacterium degrades pyrene to central intermediates through o-phthalate and the -ketoadipate Pathway. Proteomic analysis also revealed that 18 enzymes in the Pathway were upregulated more than twofold, as indicated by peptide counting when the organism was grown with pyrene; three copies of the terminal subunits of ring-hydroxylating oxygenase (NidAB2, MvanDraft_0817/0818, and PhtAaAb), dihydrodiol dehydrogenase (MvanDraft_0815), and ring cleavage dioxygenase (MvanDraft_3242) were detected only in pyrene-grown cells. The results presented here provide a comprehensive picture of pyrene metabolism in M. vanbaalenii PYR-1 and a useful framework for understanding cellular processes involved in PAH Degradation.
Nichina Gomi - One of the best experts on this subject based on the ideXlab platform.
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Degradation of the synthetic dye amaranth by the fungus bjerkandera adusta dec 1 inference of the Degradation Pathway from an analysis of decolorized products
Biodegradation, 2011Co-Authors: Nichina Gomi, Shuji Yoshida, Kazutsugu Matsumoto, Masayuki Okudomi, Hiroki Konno, Toru Hisabori, Yasushi SuganoAbstract:We examined the Degradation of amaranth, a representative azo dye, by Bjerkandera adusta Dec 1. The Degradation products were analyzed by high performance liquid chromatography (HPLC), visible absorbance, and electrospray ionization time-of-flight mass spectroscopy (ESI-TOF-MS). At the primary culture stage (3 days), the probable reaction intermediates were 1-aminonaphthalene-2,3,6-triol, 4-(hydroxyamino) naphthalene-1-ol, and 2-hydroxy-3-[2-(4-sulfophenyl) hydrazinyl] benzenesulfonic acid. After 10 days, the reaction products detected were 4-nitrophenol, phenol, 2-hydroxy-3-nitrobenzenesulfonic acid, 4-nitrobenzene sulfonic acid, and 3,4′-disulfonyl azo benzene, suggesting that no aromatic amines were created. Manganese-dependent peroxidase activity increased sharply after 3 days culture. Based on these results, we herein propose, for the first time, a Degradation Pathway for amaranth. Our results suggest that Dec 1 degrades amaranth via the combined activities of peroxidase and hydrolase and reductase action.
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Degradation of the synthetic dye amaranth by the fungus bjerkandera adusta dec 1 inference of the Degradation Pathway from an analysis of decolorized products
Biodegradation, 2011Co-Authors: Nichina Gomi, Shuji Yoshida, Kazutsugu Matsumoto, Masayuki Okudomi, Hiroki Konno, Toru Hisabori, Yasushi SuganoAbstract:We examined the Degradation of amaranth, a representative azo dye, by Bjerkandera adusta Dec 1. The Degradation products were analyzed by high performance liquid chromatography (HPLC), visible absorbance, and electrospray ionization time-of-flight mass spectroscopy (ESI-TOF-MS). At the primary culture stage (3 days), the probable reaction intermediates were 1-aminonaphthalene-2,3,6-triol, 4-(hydroxyamino) naphthalene-1-ol, and 2-hydroxy-3-[2-(4-sulfophenyl) hydrazinyl] benzenesulfonic acid. After 10 days, the reaction products detected were 4-nitrophenol, phenol, 2-hydroxy-3-nitrobenzenesulfonic acid, 4-nitrobenzene sulfonic acid, and 3,4′-disulfonyl azo benzene, suggesting that no aromatic amines were created. Manganese-dependent peroxidase activity increased sharply after 3 days culture. Based on these results, we herein propose, for the first time, a Degradation Pathway for amaranth. Our results suggest that Dec 1 degrades amaranth via the combined activities of peroxidase and hydrolase and reductase action.
Shaohua Chen - One of the best experts on this subject based on the ideXlab platform.
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Characterization of a Pyrethroid-Degrading Pseudomonas fulva Strain P31 and Biochemical Degradation Pathway of D-Phenothrin.
Frontiers in microbiology, 2018Co-Authors: Jingjing Yang, Hui Zhan, Yanmei Feng, Lianhui Zhang, Jie Liu, Yang Fang, Kaiyang Zhang, Shaohua ChenAbstract:D-phenothrin is one of the most popular pyrethroid insecticides for its broad spectrum and high insecticidal activity. However, continuous use of D-phenothrin has resulted in serious environmental contamination and raised public concern about its impact on human health. BioDegradation of D-phenothrin has never been investigated and its metabolic behaviors remain unknown. Here, a novel bacterial strain P31 was isolated from active sludge, which completely degraded (100%) D-phenothrin at 50 mg·L-1 in 72 h. Based on the morphology, 16S rRNA gene and Biolog tests, the strain was identified as Pseudomonas fulva. BioDegradation conditions were optimized as 29.5 °C and pH 7.3 by utilizing response surface methodology (RSM). Strain P31 depicted high tolerance and strong D-phenothrin Degradation ability through hydrolysis Pathway. Strain P31 degraded D-phenothrin at inhibition constant (Ki) of 482.1673 mg·L−1 and maximum specific Degradation constant (qmax) of 0.0455 h-1 whereas critical inhibitor concentration remained as 41.1189 mg·L-1. 3-Phenoxybenzaldehyde and 1,2-benzenedicarboxylic butyl dacyl ester were identified as the major intermediate metabolites of D-phenothrin Degradation Pathway through high-performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC-MS). Bioaugmentation of D-phenothrin-contaminated soils with strain P31 dramatically enhanced its Degradation, and over 75% of D-phenothrin was removed from soils within 10 days. Moreover, the strain illustrated a remarkable capacity to degrade other synthetic pyrethroids, including permethrin, cyhalothrin, β-cypermethrin, deltamethrin, fenpropathrin, and bifenthrin, exhibiting great potential in bioremediation of pyrethroid-contaminated environment.
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kinetics and novel Degradation Pathway of permethrin in acinetobacter baumannii zh 14
Frontiers in Microbiology, 2018Co-Authors: Hui Zhan, Huishan Wang, Lisheng Liao, Yanmei Feng, Xinghui Fan, Lianhui Zhang, Shaohua ChenAbstract:Persistent use of permethrin has resulted in its ubiquitous presence as a contaminant in surface streams and soils, yet little is known about the kinetics and metabolic behaviors of this pesticide. In this study, a novel bacterial strain Acinetobacter baumannii ZH-14 utilizing permethrin via partial hydrolysis Pathways was isolated from sewage sludge. Response surface methodology based on Box-Behnken design of cultural conditions was used for optimization resulting in 100% Degradation of permethrin (50 mg·L-1) within 72 h. Strain ZH-14 degraded permethrin up to a concentration of 800 mg·L-1. BioDegradation kinetics analysis indicated that permethrin Degradation by this strain was concentration dependent, with a maximum specific Degradation rate, half-saturation constant, and inhibition constant of 0.0454 h-1, 4.7912 mg·L-1, and 367.2165 mg·L-1, respectively. High-performance liquid chromatography and gas chromatography-mass spectrometry identified 3-phenoxybenzenemethanol and 3-phenoxybenzaldehyde as the major intermediate metabolites of the permethrin Degradation Pathway. Bioaugmentation of permethrin-contaminated soils with strain ZH-14 significantly enhanced Degradation, and over 85% of permethrin was degraded within 9 days with the Degradation process following the first-order kinetic model. In addition to Degradation of permethrin, strain ZH-14 was capable of degrading a large range of synthetic pyrethroids such as deltamethrin, bifenthrin, fenpropathrin, cyhalothrin, and beta-cypermethrin which are also widely used pesticides with environmental contamination problems, suggesting the promising potentials of A. baumannii ZH-14 in bioremediation of pyrethroid-contaminated terrestrial and aquatic environments.
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Characterization of a Pyrethroid-Degrading Pseudomonas fulva Strain P31 and Biochemical Degradation Pathway of D-Phenothrin
Frontiers Media S.A., 2018Co-Authors: Jingjing Yang, Hui Zhan, Yanmei Feng, Lianhui Zhang, Jie Liu, Kaiyang Zhang, Fang Yang, Shaohua ChenAbstract:D-phenothrin is one of the most popular pyrethroid insecticides for its broad spectrum and high insecticidal activity. However, continuous use of D-phenothrin has resulted in serious environmental contamination and raised public concern about its impact on human health. BioDegradation of D-phenothrin has never been investigated and its metabolic behaviors remain unknown. Here, a novel bacterial strain P31 was isolated from active sludge, which completely degraded (100%) D-phenothrin at 50 mg⋅L-1 in 72 h. Based on the morphology, 16S rRNA gene and Biolog tests, the strain was identified as Pseudomonas fulva. BioDegradation conditions were optimized as 29.5°C and pH 7.3 by utilizing response surface methodology. Strain P31 depicted high tolerance and strong D-phenothrin Degradation ability through hydrolysis Pathway. Strain P31 degraded D-phenothrin at inhibition constant (Ki) of 482.1673 mg⋅L-1 and maximum specific Degradation constant (qmax) of 0.0455 h-1 whereas critical inhibitor concentration remained as 41.1189 mg⋅L-1. The 3-Phenoxybenzaldehyde and 1,2-benzenedicarboxylic butyl dacyl ester were identified as the major intermediate metabolites of D-phenothrin Degradation Pathway through high-performance liquid chromatography and gas chromatography-mass spectrometry. Bioaugmentation of D-phenothrin-contaminated soils with strain P31 dramatically enhanced its Degradation, and over 75% of D-phenothrin was removed from soils within 10 days. Moreover, the strain illustrated a remarkable capacity to degrade other synthetic pyrethroids, including permethrin, cyhalothrin, β-cypermethrin, deltamethrin, fenpropathrin, and bifenthrin, exhibiting great potential in bioremediation of pyrethroid-contaminated environment
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Data_Sheet_1_Characterization of a Pyrethroid-Degrading Pseudomonas fulva Strain P31 and Biochemical Degradation Pathway of D-Phenothrin.PDF
2018Co-Authors: Jingjing Yang, Hui Zhan, Yanmei Feng, Lianhui Zhang, Jie Liu, Kaiyang Zhang, Fang Yang, Shaohua ChenAbstract:D-phenothrin is one of the most popular pyrethroid insecticides for its broad spectrum and high insecticidal activity. However, continuous use of D-phenothrin has resulted in serious environmental contamination and raised public concern about its impact on human health. BioDegradation of D-phenothrin has never been investigated and its metabolic behaviors remain unknown. Here, a novel bacterial strain P31 was isolated from active sludge, which completely degraded (100%) D-phenothrin at 50 mg⋅L-1 in 72 h. Based on the morphology, 16S rRNA gene and Biolog tests, the strain was identified as Pseudomonas fulva. BioDegradation conditions were optimized as 29.5°C and pH 7.3 by utilizing response surface methodology. Strain P31 depicted high tolerance and strong D-phenothrin Degradation ability through hydrolysis Pathway. Strain P31 degraded D-phenothrin at inhibition constant (Ki) of 482.1673 mg⋅L-1 and maximum specific Degradation constant (qmax) of 0.0455 h-1 whereas critical inhibitor concentration remained as 41.1189 mg⋅L-1. The 3-Phenoxybenzaldehyde and 1,2-benzenedicarboxylic butyl dacyl ester were identified as the major intermediate metabolites of D-phenothrin Degradation Pathway through high-performance liquid chromatography and gas chromatography-mass spectrometry. Bioaugmentation of D-phenothrin-contaminated soils with strain P31 dramatically enhanced its Degradation, and over 75% of D-phenothrin was removed from soils within 10 days. Moreover, the strain illustrated a remarkable capacity to degrade other synthetic pyrethroids, including permethrin, cyhalothrin, β-cypermethrin, deltamethrin, fenpropathrin, and bifenthrin, exhibiting great potential in bioremediation of pyrethroid-contaminated environment.
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characterization of a novel cyfluthrin degrading bacterial strain brevibacterium aureum and its biochemical Degradation Pathway
Bioresource Technology, 2013Co-Authors: Shaohua Chen, Lianhui Zhang, Yihu Dong, Changqing Chang, Yinyue Deng, Xi Fen Zhang, Guohua Zhong, Haiwei SongAbstract:Brevibacterium aureum DG-12, a new bacterial strain isolated from active sludge, was able to degrade and utilize cyfluthrin as a growth substrate in the mineral medium. Response surface methodology using central composite rotatable design of cultural conditions was successfully employed for optimization resulting in 88.6% Degradation of cyfluthrin (50mgL(-1)) within 5days. The bacterium degraded cyfluthrin by cleavage of both the carboxylester linkage and diaryl bond to form 2,2,3,3-tetramethyl-cyclopropanemethanol, 4-fluoro-3-phenexy-benzoic acid, 3,5-dimethoxy phenol, and phenol, and subsequently transformed these compounds with a maximum specific Degradation rate, half-saturation constant and inhibition constant of 1.0384day(-1), 20.4967mgL(-1), and 141.9013mgL(-1), respectively. A novel Degradation Pathway for cyfluthrin was proposed based on analysis of these metabolites. In addition, this strain was found capable of degrading a wide range of synthetic pyrethroid insecticides. Our results suggest that B. aureum DG-12 may be an ideal microorganism for bioremediation of the pyrethroid-contaminated environments.