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Thorsten Schwark - One of the best experts on this subject based on the ideXlab platform.

  • a new multiplex pcr comprising autosomal and y specific strs and mitochondrial dna to analyze highly Degraded Material
    Forensic Science International-genetics, 2009
    Co-Authors: Nicole Von Wurmbschwark, Anke Heinrich, Eva Simeoni, Andrea Preusseprange, Thomas C G Bosch, Thorsten Schwark
    Abstract:

    The analysis of short tandem repeats is one of the most powerful tools in forensic genetics. Forensic practice sometimes requires the individualization of samples that may contain only highly Degraded nuclear DNA, mitochondrial DNA or PCR inhibitors that hamper DNA amplification. We designed a new multiplex PCR with reduced size amplicons (<200 bp), providing a double sex determination (amelogenin plus two Y-STRs), the detection of two autosomal markers and the amplification of mitochondrial specific fragments from the hypervariable region 1 (HVI). Additionally, a quality sensor was developed to check for the presence of any PCR inhibitors.The new multiplex PCR shows a reproducible detection threshold down to 25 pg and gives signals even out of highly Degraded Materials. All signals are reproducible and reliable as it could be shown in comparison to results from commercially available STR multiplex-PCRs. In no case DNA fragments were detectable using any other assay when the quality sensor was not detectable. There was a good correlation between detection of mitochondrial specific fragments in the multiplex-PCR and success of subsequent sequencing of HVI region. The same could be shown for STR analysis: Most samples successfully analyzed in our PCR yielded at least a partial STR profile using a commercial STR kit. We present an assay that allows an easy, reliable, and cost efficient evaluation of DNA sample quality combined with a first rough sample individualization and sex determination suitable for forensic purposes. This assay may help the forensic lab personnel to decide on further sample processing. (C) 2008 Elsevier Ireland Ltd. All rights reserved.

  • A new multiplex-PCR comprising autosomal and y-specific STRs and mitochondrial DNA to analyze highly Degraded Material
    Forensic Science International: Genetics, 2009
    Co-Authors: Nicole Von Wurmb-schwark, Andrea Preusse-prange, Anke Heinrich, Eva Simeoni, Thomas Bosch, Thorsten Schwark
    Abstract:

    The analysis of short tandem repeats is one of the most powerful tools in forensic genetics. Forensic practice sometimes requires the individualization of samples that may contain only highly Degraded nuclear DNA, mitochondrial DNA or PCR inhibitors that hamper DNA amplification. We designed a new multiplex PCR with reduced size amplicons (

Brendan C Okelly - One of the best experts on this subject based on the ideXlab platform.

  • geotechnical properties of municipal sewage sludge
    Geotechnical and Geological Engineering, 2006
    Co-Authors: Brendan C Okelly
    Abstract:

    The geotechnical properties of municipal sewage sludge, in particular those pertinent to the handling and landfilling of the Material, are presented. Index, drying, compaction, shear strength and consolidation tests were conducted on the Material at different states of biodegradation. The organic content and specific gravity of solids were found to be inversely related, with typical organic contents of 50–70% and specific gravity of solids values of 1.55–1.80. The density of the compacted Material was low in comparison with mineral soils. Standard Proctor compaction yielded a maximum dry density of 0.56 tonne/m3 at 85% water content. Laboratory vane-shear and triaxial compression tests indicated that, below about 180% water content, the shear strength of the sludge Material increased exponentially with reducing water content. Consolidated-undrained triaxial compression tests on the pasteurised sludge Material indicated an effective angle of shearing resistance of 32° for the moderately Degraded Material and 37° for the strongly Degraded Material. Biogas was produced at rates of up to 0.33 L/day/kg slurry due to ongoing biodegradation and the resulting pore pressure response must be taken into account in any stress analysis. Consolidation tests using the hydraulic consolidation cell, oedometer and triaxial apparatus indicated that the sludge Material was highly compressible although practically impermeable, for example the coefficient of permeability for the moderately Degraded slurry was of the order of 10−9m/s. However, creep deformation was significant with typical coefficient of secondary compression values of 0.02–0.08 for the compacted Material. A more free-draining Material was produced at higher states of biodegradation.

  • consolidation properties of a dewatered municipal sewage sludge
    Canadian Geotechnical Journal, 2005
    Co-Authors: Brendan C Okelly
    Abstract:

    The consolidation properties of a dewatered municipal sewage sludge were studied using the oedometer, hydraulic consolidation cell, and triaxial apparatus. Bioactive and stabilized test specimens of dried-compacted Material and slurry Material at different states of biodegradation (LOI = 55–70%, where LOI is the loss on ignition) were consolidated under applied stresses of 3–400 kPa. The rate of biogas production and the resulting pore pressure response of the unsaturated Material were also studied for different specimen drainage conditions. The sludge Material largely consisted of organic clay-sized particles, and although highly compressible, the Material was practically impermeable (k = 10–9–10–11 m/s for slurry Material, where k is the coefficient of permeability). Primary consolidation generally constituted only a small part of the overall deformation response for moderately Degraded Material (LOI 70%). Secondary compression was dominant, and for the bioactive Material, included a significant contrib...

Anke Heinrich - One of the best experts on this subject based on the ideXlab platform.

  • a new multiplex pcr comprising autosomal and y specific strs and mitochondrial dna to analyze highly Degraded Material
    Forensic Science International-genetics, 2009
    Co-Authors: Nicole Von Wurmbschwark, Anke Heinrich, Eva Simeoni, Andrea Preusseprange, Thomas C G Bosch, Thorsten Schwark
    Abstract:

    The analysis of short tandem repeats is one of the most powerful tools in forensic genetics. Forensic practice sometimes requires the individualization of samples that may contain only highly Degraded nuclear DNA, mitochondrial DNA or PCR inhibitors that hamper DNA amplification. We designed a new multiplex PCR with reduced size amplicons (<200 bp), providing a double sex determination (amelogenin plus two Y-STRs), the detection of two autosomal markers and the amplification of mitochondrial specific fragments from the hypervariable region 1 (HVI). Additionally, a quality sensor was developed to check for the presence of any PCR inhibitors.The new multiplex PCR shows a reproducible detection threshold down to 25 pg and gives signals even out of highly Degraded Materials. All signals are reproducible and reliable as it could be shown in comparison to results from commercially available STR multiplex-PCRs. In no case DNA fragments were detectable using any other assay when the quality sensor was not detectable. There was a good correlation between detection of mitochondrial specific fragments in the multiplex-PCR and success of subsequent sequencing of HVI region. The same could be shown for STR analysis: Most samples successfully analyzed in our PCR yielded at least a partial STR profile using a commercial STR kit. We present an assay that allows an easy, reliable, and cost efficient evaluation of DNA sample quality combined with a first rough sample individualization and sex determination suitable for forensic purposes. This assay may help the forensic lab personnel to decide on further sample processing. (C) 2008 Elsevier Ireland Ltd. All rights reserved.

  • A new multiplex-PCR comprising autosomal and y-specific STRs and mitochondrial DNA to analyze highly Degraded Material
    Forensic Science International: Genetics, 2009
    Co-Authors: Nicole Von Wurmb-schwark, Andrea Preusse-prange, Anke Heinrich, Eva Simeoni, Thomas Bosch, Thorsten Schwark
    Abstract:

    The analysis of short tandem repeats is one of the most powerful tools in forensic genetics. Forensic practice sometimes requires the individualization of samples that may contain only highly Degraded nuclear DNA, mitochondrial DNA or PCR inhibitors that hamper DNA amplification. We designed a new multiplex PCR with reduced size amplicons (

Eva Simeoni - One of the best experts on this subject based on the ideXlab platform.

  • a new multiplex pcr comprising autosomal and y specific strs and mitochondrial dna to analyze highly Degraded Material
    Forensic Science International-genetics, 2009
    Co-Authors: Nicole Von Wurmbschwark, Anke Heinrich, Eva Simeoni, Andrea Preusseprange, Thomas C G Bosch, Thorsten Schwark
    Abstract:

    The analysis of short tandem repeats is one of the most powerful tools in forensic genetics. Forensic practice sometimes requires the individualization of samples that may contain only highly Degraded nuclear DNA, mitochondrial DNA or PCR inhibitors that hamper DNA amplification. We designed a new multiplex PCR with reduced size amplicons (<200 bp), providing a double sex determination (amelogenin plus two Y-STRs), the detection of two autosomal markers and the amplification of mitochondrial specific fragments from the hypervariable region 1 (HVI). Additionally, a quality sensor was developed to check for the presence of any PCR inhibitors.The new multiplex PCR shows a reproducible detection threshold down to 25 pg and gives signals even out of highly Degraded Materials. All signals are reproducible and reliable as it could be shown in comparison to results from commercially available STR multiplex-PCRs. In no case DNA fragments were detectable using any other assay when the quality sensor was not detectable. There was a good correlation between detection of mitochondrial specific fragments in the multiplex-PCR and success of subsequent sequencing of HVI region. The same could be shown for STR analysis: Most samples successfully analyzed in our PCR yielded at least a partial STR profile using a commercial STR kit. We present an assay that allows an easy, reliable, and cost efficient evaluation of DNA sample quality combined with a first rough sample individualization and sex determination suitable for forensic purposes. This assay may help the forensic lab personnel to decide on further sample processing. (C) 2008 Elsevier Ireland Ltd. All rights reserved.

  • A new multiplex-PCR comprising autosomal and y-specific STRs and mitochondrial DNA to analyze highly Degraded Material
    Forensic Science International: Genetics, 2009
    Co-Authors: Nicole Von Wurmb-schwark, Andrea Preusse-prange, Anke Heinrich, Eva Simeoni, Thomas Bosch, Thorsten Schwark
    Abstract:

    The analysis of short tandem repeats is one of the most powerful tools in forensic genetics. Forensic practice sometimes requires the individualization of samples that may contain only highly Degraded nuclear DNA, mitochondrial DNA or PCR inhibitors that hamper DNA amplification. We designed a new multiplex PCR with reduced size amplicons (

Nicole Von Wurmbschwark - One of the best experts on this subject based on the ideXlab platform.

  • a new multiplex pcr comprising autosomal and y specific strs and mitochondrial dna to analyze highly Degraded Material
    Forensic Science International-genetics, 2009
    Co-Authors: Nicole Von Wurmbschwark, Anke Heinrich, Eva Simeoni, Andrea Preusseprange, Thomas C G Bosch, Thorsten Schwark
    Abstract:

    The analysis of short tandem repeats is one of the most powerful tools in forensic genetics. Forensic practice sometimes requires the individualization of samples that may contain only highly Degraded nuclear DNA, mitochondrial DNA or PCR inhibitors that hamper DNA amplification. We designed a new multiplex PCR with reduced size amplicons (<200 bp), providing a double sex determination (amelogenin plus two Y-STRs), the detection of two autosomal markers and the amplification of mitochondrial specific fragments from the hypervariable region 1 (HVI). Additionally, a quality sensor was developed to check for the presence of any PCR inhibitors.The new multiplex PCR shows a reproducible detection threshold down to 25 pg and gives signals even out of highly Degraded Materials. All signals are reproducible and reliable as it could be shown in comparison to results from commercially available STR multiplex-PCRs. In no case DNA fragments were detectable using any other assay when the quality sensor was not detectable. There was a good correlation between detection of mitochondrial specific fragments in the multiplex-PCR and success of subsequent sequencing of HVI region. The same could be shown for STR analysis: Most samples successfully analyzed in our PCR yielded at least a partial STR profile using a commercial STR kit. We present an assay that allows an easy, reliable, and cost efficient evaluation of DNA sample quality combined with a first rough sample individualization and sex determination suitable for forensic purposes. This assay may help the forensic lab personnel to decide on further sample processing. (C) 2008 Elsevier Ireland Ltd. All rights reserved.