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Kiyotaka Okada - One of the best experts on this subject based on the ideXlab platform.
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the defective in anther Dehiscence1 gene encodes a novel phospholipase a1 catalyzing the initial step of jasmonic acid biosynthesis which synchronizes pollen maturation anther Dehiscence and flower opening in arabidopsis
The Plant Cell, 2001Co-Authors: Sumie Ishiguro, Akiko Kawaioda, Junichi Ueda, Ikuo Nishida, Kiyotaka OkadaAbstract:The Arabidopsis mutant defective in anther Dehiscence1 (dad1) shows defects in anther Dehiscence, pollen maturation, and flower opening. The defects were rescued by the exogenous application of jasmonic acid (JA) or linolenic acid, which is consistent with the reduced accumulation of JA in the dad1 flower buds. We identified the DAD1 gene by T-DNA tagging, which is characteristic to a putative N-terminal transit peptide and a conserved motif found in lipase active sites. DAD1 protein expressed in Escherichia coli hydrolyzed phospholipids in an sn-1–specific manner, and DAD1–green fluorescent protein fusion protein expressed in leaf epidermal cells localized predominantly in chloroplasts. These results indicate that the DAD1 protein is a chloroplastic phospholipase A1 that catalyzes the initial step of JA biosynthesis. DAD1 promoter::β-glucuronidase analysis revealed that the expression of DAD1 is restricted in the stamen filaments. A model is presented in which JA synthesized in the filaments regulates the water transport in stamens and petals.
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the defective in anther Dehiscence1 gene encodes a novel phospholipase a1 catalyzing the initial step of jasmonic acid biosynthesis which synchronizes pollen maturation anther Dehiscence and flower opening in arabidopsis
The Plant Cell, 2001Co-Authors: Sumie Ishiguro, Akiko Kawaioda, Junichi Ueda, Ikuo Nishida, Kiyotaka OkadaAbstract:The Arabidopsis mutant defective in anther Dehiscence1 (dad1) shows defects in anther Dehiscence, pollen maturation, and flower opening. The defects were rescued by the exogenous application of jasmonic acid (JA) or linolenic acid, which is consistent with the reduced accumulation of JA in the dad1 flower buds. We identified the DAD1 gene by T-DNA tagging, which is characteristic to a putative N-terminal transit peptide and a conserved motif found in lipase active sites. DAD1 protein expressed in Escherichia coli hydrolyzed phospholipids in an sn-1-specific manner, and DAD1-green fluorescent protein fusion protein expressed in leaf epidermal cells localized predominantly in chloroplasts. These results indicate that the DAD1 protein is a chloroplastic phospholipase A1 that catalyzes the initial step of JA biosynthesis. DAD1 promoter::beta-glucuronidase analysis revealed that the expression of DAD1 is restricted in the stamen filaments. A model is presented in which JA synthesized in the filaments regulates the water transport in stamens and petals.
Sumie Ishiguro - One of the best experts on this subject based on the ideXlab platform.
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the defective in anther Dehiscence1 gene encodes a novel phospholipase a1 catalyzing the initial step of jasmonic acid biosynthesis which synchronizes pollen maturation anther Dehiscence and flower opening in arabidopsis
The Plant Cell, 2001Co-Authors: Sumie Ishiguro, Akiko Kawaioda, Junichi Ueda, Ikuo Nishida, Kiyotaka OkadaAbstract:The Arabidopsis mutant defective in anther Dehiscence1 (dad1) shows defects in anther Dehiscence, pollen maturation, and flower opening. The defects were rescued by the exogenous application of jasmonic acid (JA) or linolenic acid, which is consistent with the reduced accumulation of JA in the dad1 flower buds. We identified the DAD1 gene by T-DNA tagging, which is characteristic to a putative N-terminal transit peptide and a conserved motif found in lipase active sites. DAD1 protein expressed in Escherichia coli hydrolyzed phospholipids in an sn-1–specific manner, and DAD1–green fluorescent protein fusion protein expressed in leaf epidermal cells localized predominantly in chloroplasts. These results indicate that the DAD1 protein is a chloroplastic phospholipase A1 that catalyzes the initial step of JA biosynthesis. DAD1 promoter::β-glucuronidase analysis revealed that the expression of DAD1 is restricted in the stamen filaments. A model is presented in which JA synthesized in the filaments regulates the water transport in stamens and petals.
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the defective in anther Dehiscence1 gene encodes a novel phospholipase a1 catalyzing the initial step of jasmonic acid biosynthesis which synchronizes pollen maturation anther Dehiscence and flower opening in arabidopsis
The Plant Cell, 2001Co-Authors: Sumie Ishiguro, Akiko Kawaioda, Junichi Ueda, Ikuo Nishida, Kiyotaka OkadaAbstract:The Arabidopsis mutant defective in anther Dehiscence1 (dad1) shows defects in anther Dehiscence, pollen maturation, and flower opening. The defects were rescued by the exogenous application of jasmonic acid (JA) or linolenic acid, which is consistent with the reduced accumulation of JA in the dad1 flower buds. We identified the DAD1 gene by T-DNA tagging, which is characteristic to a putative N-terminal transit peptide and a conserved motif found in lipase active sites. DAD1 protein expressed in Escherichia coli hydrolyzed phospholipids in an sn-1-specific manner, and DAD1-green fluorescent protein fusion protein expressed in leaf epidermal cells localized predominantly in chloroplasts. These results indicate that the DAD1 protein is a chloroplastic phospholipase A1 that catalyzes the initial step of JA biosynthesis. DAD1 promoter::beta-glucuronidase analysis revealed that the expression of DAD1 is restricted in the stamen filaments. A model is presented in which JA synthesized in the filaments regulates the water transport in stamens and petals.
H.p, Chandra Isabella - One of the best experts on this subject based on the ideXlab platform.
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Faktor-Faktor yang Berhubungan dengan Kejadian Wound Dehiscence pada Pasien Post Laparatomi
Universitas Padjadjaran, 2017Co-Authors: Ningrum, Tita Puspita, Mediani, Henny Suzana, H.p, Chandra IsabellaAbstract:Wound Dehiscence sering terjadi setelah pembedahan mayor abdomen menimbulkan tingkat morbiditas dan mortalitas yang tinggi. Wound Dehiscence dapat menimbulkan stress, eviserasi, reoperasi, gangguan citra tubuh, meningkatnya lama rawat dan biaya rawat, menurunkan kualitas hidup pasien serta kematian sehingga perlu menangani faktor yang mempengaruhi kejadian wound Dehiscence. Tujuan dari penelitian ini adalah untuk menganalisis faktor-faktor yang berhubungan dengan kejadian wound Dehiscence pada pasien dewasa post laparatomi di RSUP Dr Hasan Sadikin Bandung. Metode penelitian menggunakan analitik korelasi dengan pendekatan cross sectional. Sampel yang digunakan berjumlah 40 orang yang diambil dengan menggunakan consecutive sampling. Pengumpulan data dengan cara wawancara, observasi dan studi dokumentasi. Analisis univariat menggunakan distribusi frekuensi dan analisis bivariat menggunakan uji Chi Square. Hasil penelitian menunjukkan kejadian wound Dehiscence terjadi ketika perawatan di rumah (35%). Hasil analisis bivariat menunjukkan adanya hubungan yang signifikan antara infeksi luka (p=0,0001), operasi emergensi (p = 0,020), hipoalbumin (p=0,037), anemia (p = 0,028), status nutrisi (0,010), dan adanya penyakit penyerta (p = 0,008) dengan kejadian wound Dehiscence, serta tidak ada hubungan yang signifikan antara faktor usia (p = 0,581) dan jenis kelamin (p= 0,604) dengan kejadian wound Dehiscence. Penting bagi perawat untuk mengidentifikasi potensial faktor risiko wound Dehiscence pada pasien yang dilakukan operasi laparatomi dan segera melakukan intervensi yang diperlukan untuk mencegah terjadinya komplikasi wound Dehiscence, diantaranya dengan melakukan discharge planning terkait perawatan luka dan pentingnya asupan protein yang adekuat supaya bisa dikenali ditahab mana terjadinya wound Dehiscence.Kata kunci: Pasien, post laparatomi, wound Dehiscence. Factors correlating of Wound Dehiscence in Patients after Laparatomi at Dr Hasan Sadikin General Hospital BandungAbsractWound Dehiscence is often occurred after major abdominal surgery which impacts on morbidity and mortality rates and significantly contributes to prolonged hospital stays, implicit and explicit costs, associate with psychosocial stressor on patients, evisceration re-surgical operation, and may affect to quality of life patients. It is therefore necessary to identify factors affecting wound Dehiscence. The aims of the study was to analyze factors correlating of post-operative wound Dehiscence in adult patients at Dr Hasan Sadikin general hospital. Correlational analytic with cross sectional approach was used in this study. 40 patients were selected to be participated in this study by using consecutive sampling. Observations, interviews and study documents were conducted in data collection process. Univariate and Bivariate analysis with Chi Square were performed to analyze the data. Results of the study identified than wound Dehiscence were occurred during patients at home (35%). Result of analysis bivariate showed that there was a significance correlation between wound infection (p=0, 0001), surgical emergency (p = 0,020), hypo albumin (p=0,037), anemia (p = 0,028), nutrition status (0,010), and other illness (p = 0,008) with wound Dehiscence. Whereas, there was no correlation significantly between age factor (p = 0,581) and gender (p= 0,604) with wound Dehiscence. It is important for nurses to identify potential risk factors of wound Dehiscence in patients after post-operative laparotomy and prevent complication of wound Dehiscence by doing discharge planning especially in term of wound care and the need of taking protein consumption adequately to avoid wound Dehiscence.Key words: Adult patients, post-laparatomi, wound Dehiscence
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Faktor-Faktor yang Berhubungan dengan Kejadian Wound Dehiscence pada Pasien Post Laparatomi
Padjadjaran University, 2017Co-Authors: Ningrum T. P., Mediani H. S., H.p, Chandra IsabellaAbstract:Wound Dehiscence sering terjadi setelah pembedahan mayor abdomen menimbulkan tingkat morbiditas dan mortalitas yang tinggi. Wound Dehiscence dapat menimbulkan stress, eviserasi, reoperasi, gangguan citra tubuh, meningkatnya lama rawat dan biaya rawat, menurunkan kualitas hidup pasien serta kematian sehingga perlu menangani faktor yang mempengaruhi kejadian wound Dehiscence. Tujuan dari penelitian ini adalah untuk menganalisis faktor-faktor yang berhubungan dengan kejadian wound Dehiscence pada pasien dewasa post laparatomi di RSUP Dr Hasan Sadikin Bandung. Metode penelitian menggunakan analitik korelasi dengan pendekatan cross sectional. Sampel yang digunakan berjumlah 40 orang yang diambil dengan menggunakan consecutive sampling. Pengumpulan data dengan cara wawancara, observasi dan studi dokumentasi. Analisis univariat menggunakan distribusi frekuensi dan analisis bivariat menggunakan uji Chi Square. Hasil penelitian menunjukkan kejadian wound Dehiscence terjadi ketika perawatan di rumah (35%). Hasil analisis bivariat menunjukkan adanya hubungan yang signifikan antara infeksi luka (p=0,0001), operasi emergensi (p = 0,020), hipoalbumin (p=0,037), anemia (p = 0,028), status nutrisi (0,010), dan adanya penyakit penyerta (p = 0,008) dengan kejadian wound Dehiscence, serta tidak ada hubungan yang signifikan antara faktor usia (p = 0,581) dan jenis kelamin (p= 0,604) dengan kejadian wound Dehiscence. Penting bagi perawat untuk mengidentifikasi potensial faktor risiko wound Dehiscence pada pasien yang dilakukan operasi laparatomi dan segera melakukan intervensi yang diperlukan untuk mencegah terjadinya komplikasi wound Dehiscence, diantaranya dengan melakukan discharge planning terkait perawatan luka dan pentingnya asupan protein yang adekuat supaya bisa dikenali ditahab mana terjadinya wound Dehiscence.Kata kunci: Pasien, post laparatomi, wound Dehiscence. Factors correlating of Wound Dehiscence in Patients after Laparatomi at Dr Hasan Sadikin General Hospital BandungAbsractWound Dehiscence is often occurred after major abdominal surgery which impacts on morbidity and mortality rates and significantly contributes to prolonged hospital stays, implicit and explicit costs, associate with psychosocial stressor on patients, evisceration re-surgical operation, and may affect to quality of life patients. It is therefore necessary to identify factors affecting wound Dehiscence. The aims of the study was to analyze factors correlating of post-operative wound Dehiscence in adult patients at Dr Hasan Sadikin general hospital. Correlational analytic with cross sectional approach was used in this study. 40 patients were selected to be participated in this study by using consecutive sampling. Observations, interviews and study documents were conducted in data collection process. Univariate and Bivariate analysis with Chi Square were performed to analyze the data. Results of the study identified than wound Dehiscence were occurred during patients at home (35%). Result of analysis bivariate showed that there was a significance correlation between wound infection (p=0, 0001), surgical emergency (p = 0,020), hypo albumin (p=0,037), anemia (p = 0,028), nutrition status (0,010), and other illness (p = 0,008) with wound Dehiscence. Whereas, there was no correlation significantly between age factor (p = 0,581) and gender (p= 0,604) with wound Dehiscence. It is important for nurses to identify potential risk factors of wound Dehiscence in patients after post-operative laparotomy and prevent complication of wound Dehiscence by doing discharge planning especially in term of wound care and the need of taking protein consumption adequately to avoid wound Dehiscence
R. W. Günther - One of the best experts on this subject based on the ideXlab platform.
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Posterior semicircular canal Dehiscence: a morphologic cause of vertigo similar to superior semicircular canal Dehiscence
European Radiology, 2003Co-Authors: G. A. Krombach, E. Dimartino, T. Schmitz-rode, A. Prescher, P. Haage, S. Kinzel, R. W. GüntherAbstract:The aim of this study was to assess imaging findings of posterior semicircular Dehiscence on computed tomography and to evaluate incidence of posterior and superior semicircular canal Dehiscence in patients presenting with vertigo, sensorineuronal hearing loss or in a control group without symptoms related to the inner ear. Computed tomography was performed in 507 patients presenting either with vertigo ( n =128; 23 of these patients suffered also from sensorineuronal hearing loss), other symptoms related to the inner ear, such as hearing loss or tinnitus ( n =183) or symptoms unrelated to the labyrinth ( n =196). All images were reviewed for presence of Dehiscence of the bone, overlying the semicircular canals. Twenty-nine patients had superior semicircular canal Dehiscence. Of these patients, 83% presented with vertigo, 10% with hearing loss or tinnitus and the remaining 7% with symptoms unrelated to the inner ear. In 23 patients Dehiscence of the posterior semicircular canal was encountered. Of these patients, 86% presented with vertigo, 9% with hearing loss or tinnitus and 5% with symptoms unrelated to the inner ear. Defects of the bony overly are found at the posterior semicircular canal, in addition to the recently introduced superior canal Dehiscence syndrome. Significant prevalence of vertigo in these patients suggests that posterior semicircular canal Dehiscence can cause vertigo, similar to superior semicircular canal Dehiscence.
Akiko Kawaioda - One of the best experts on this subject based on the ideXlab platform.
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the defective in anther Dehiscence1 gene encodes a novel phospholipase a1 catalyzing the initial step of jasmonic acid biosynthesis which synchronizes pollen maturation anther Dehiscence and flower opening in arabidopsis
The Plant Cell, 2001Co-Authors: Sumie Ishiguro, Akiko Kawaioda, Junichi Ueda, Ikuo Nishida, Kiyotaka OkadaAbstract:The Arabidopsis mutant defective in anther Dehiscence1 (dad1) shows defects in anther Dehiscence, pollen maturation, and flower opening. The defects were rescued by the exogenous application of jasmonic acid (JA) or linolenic acid, which is consistent with the reduced accumulation of JA in the dad1 flower buds. We identified the DAD1 gene by T-DNA tagging, which is characteristic to a putative N-terminal transit peptide and a conserved motif found in lipase active sites. DAD1 protein expressed in Escherichia coli hydrolyzed phospholipids in an sn-1–specific manner, and DAD1–green fluorescent protein fusion protein expressed in leaf epidermal cells localized predominantly in chloroplasts. These results indicate that the DAD1 protein is a chloroplastic phospholipase A1 that catalyzes the initial step of JA biosynthesis. DAD1 promoter::β-glucuronidase analysis revealed that the expression of DAD1 is restricted in the stamen filaments. A model is presented in which JA synthesized in the filaments regulates the water transport in stamens and petals.
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the defective in anther Dehiscence1 gene encodes a novel phospholipase a1 catalyzing the initial step of jasmonic acid biosynthesis which synchronizes pollen maturation anther Dehiscence and flower opening in arabidopsis
The Plant Cell, 2001Co-Authors: Sumie Ishiguro, Akiko Kawaioda, Junichi Ueda, Ikuo Nishida, Kiyotaka OkadaAbstract:The Arabidopsis mutant defective in anther Dehiscence1 (dad1) shows defects in anther Dehiscence, pollen maturation, and flower opening. The defects were rescued by the exogenous application of jasmonic acid (JA) or linolenic acid, which is consistent with the reduced accumulation of JA in the dad1 flower buds. We identified the DAD1 gene by T-DNA tagging, which is characteristic to a putative N-terminal transit peptide and a conserved motif found in lipase active sites. DAD1 protein expressed in Escherichia coli hydrolyzed phospholipids in an sn-1-specific manner, and DAD1-green fluorescent protein fusion protein expressed in leaf epidermal cells localized predominantly in chloroplasts. These results indicate that the DAD1 protein is a chloroplastic phospholipase A1 that catalyzes the initial step of JA biosynthesis. DAD1 promoter::beta-glucuronidase analysis revealed that the expression of DAD1 is restricted in the stamen filaments. A model is presented in which JA synthesized in the filaments regulates the water transport in stamens and petals.
