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Melvin J Oliver - One of the best experts on this subject based on the ideXlab platform.
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sporobolus stapfianus insights into desiccation tolerance in the resurrection grasses from linking transcriptomics to metabolomics
BMC Plant Biology, 2017Co-Authors: Abou Yobi, Karen Schlauch, Richard L Tillett, Catherine Espinoza, Bernard W M Wone, John C Cushman, Melvin J OliverAbstract:Understanding the response of resurrection angiosperms to Dehydration and rehydration is critical for deciphering the mechanisms of how plants cope with the rigors of water loss from their vegetative tissues. We have focused our studies on the C4 resurrection grass, Sporobolus stapfianus Gandoger, as a member of a group of important forage grasses. We have combined non-targeted metabolomics with transcriptomics, via a NimbleGen array platform, to develop an understanding of how gene expression and metabolite profiles can be linked to generate a more detailed mechanistic appreciation of the cellular response to both desiccation and rehydration. The rehydration transcriptome and metabolome are primarily geared towards the rapid return of photosynthesis, energy metabolism, protein turnover, and protein synthesis during the rehydration phase. However, there are some metabolites associated with ROS protection that remain elevated during rehydration, most notably the tocopherols. The analysis of the Dehydration transcriptome reveals a strong concordance between transcript abundance and the associated metabolite abundance reported earlier, but only in responses that are directly related to cellular protection during Dehydration: carbohydrate metabolism and redox homeostasis. The transcriptome response also provides strong support for the involvement of cellular protection processes as exemplified by the increases in the abundance of transcripts encoding late embryogenesis abundant (LEA) proteins, anti-oxidant enzymes, early light-induced proteins (ELIP) proteins, and cell-wall modification enzymes. There is little concordance between transcript and metabolite abundance for processes such as amino acid metabolism that do not appear to contribute directly to cellular protection, but are nonetheless important for the desiccation tolerant phenotype of S. stapfianus. The transcriptomes of both Dehydration and rehydration offer insight into the complexity of the regulation of responses to these processes that involve complex signaling pathways and associated transcription factors. ABA appears to be important in the control of gene expression in both the latter stages of the Dehydration and the early stages of rehydration. These findings add to the growing body of information detailing how plants tolerate and survive the severe cellular perturbations of Dehydration, desiccation, and rehydration.
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sporobolus stapfianus insights into desiccation tolerance in the resurrection grasses from linking transcriptomics to metabolomics
BMC Plant Biology, 2017Co-Authors: Abou Yobi, Karen Schlauch, Richard L Tillett, Catherine Espinoza, Bernard W M Wone, John C Cushman, Melvin J OliverAbstract:Understanding the response of resurrection angiosperms to Dehydration and rehydration is critical for deciphering the mechanisms of how plants cope with the rigors of water loss from their vegetative tissues. We have focused our studies on the C4 resurrection grass, Sporobolus stapfianus Gandoger, as a member of a group of important forage grasses. We have combined non-targeted metabolomics with transcriptomics, via a NimbleGen array platform, to develop an understanding of how gene expression and metabolite profiles can be linked to generate a more detailed mechanistic appreciation of the cellular response to both desiccation and rehydration. The rehydration transcriptome and metabolome are primarily geared towards the rapid return of photosynthesis, energy metabolism, protein turnover, and protein synthesis during the rehydration phase. However, there are some metabolites associated with ROS protection that remain elevated during rehydration, most notably the tocopherols. The analysis of the Dehydration transcriptome reveals a strong concordance between transcript abundance and the associated metabolite abundance reported earlier, but only in responses that are directly related to cellular protection during Dehydration: carbohydrate metabolism and redox homeostasis. The transcriptome response also provides strong support for the involvement of cellular protection processes as exemplified by the increases in the abundance of transcripts encoding late embryogenesis abundant (LEA) proteins, anti-oxidant enzymes, early light-induced proteins (ELIP) proteins, and cell-wall modification enzymes. There is little concordance between transcript and metabolite abundance for processes such as amino acid metabolism that do not appear to contribute directly to cellular protection, but are nonetheless important for the desiccation tolerant phenotype of S. stapfianus. The transcriptomes of both Dehydration and rehydration offer insight into the complexity of the regulation of responses to these processes that involve complex signaling pathways and associated transcription factors. ABA appears to be important in the control of gene expression in both the latter stages of the Dehydration and the early stages of rehydration. These findings add to the growing body of information detailing how plants tolerate and survive the severe cellular perturbations of Dehydration, desiccation, and rehydration.
Mohamad Suhail Rafudeen - One of the best experts on this subject based on the ideXlab platform.
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glycerolipid analysis during desiccation and recovery of the resurrection plant xerophyta humilis bak dur and schinz
Plant Cell and Environment, 2018Co-Authors: Freedom Tshabuse, Lydie Humbert, Deborah Moura, Dominique Rainteau, Christophe Espinasse, Sébastien Acket, Franck Merlier, J. Mark Farrant, Abdelghani Idrissi, Mohamad Suhail RafudeenAbstract:: Xerophyta humilis is a poikilochlorophyllous monocot resurrection plant used as a model to study vegetative desiccation tolerance. Dehydration imposes tension and ultimate loss of integrity of membranes in desiccation sensitive species. We investigated the predominant molecular species of glycerolipids present in root and leaf tissues, using multiple reaction monitoring mass spectrometry, and then analysed changes therein during Dehydration and subsequent rehydration of whole plants. The presence of fatty acids with long carbon chains and with odd numbers of carbons were detected and confirmed by gas chromatography. Dehydration of both leaves and roots resulted in an increase in species containing polyunsaturated fatty acids and a decrease in disaturated species. Upon rehydration, lipid saturation was reversed, with this being initiated immediately upon watering in roots but only 12-24 hr later in leaves. Relative levels of species with short-chained odd-numbered saturated fatty acids decreased during Dehydration and increased during rehydration, whereas the reverse trend was observed for long-chained fatty acids. X. humilis has a unique lipid composition, this report being one of the few to demonstrate the presence of odd-numbered fatty acids in plant phosphoglycerolipids.
Kenneth B Storey - One of the best experts on this subject based on the ideXlab platform.
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role of antioxidant defenses in the tolerance of severe Dehydration by anurans the case of the leopard frog rana pipiens
Molecular and Cellular Biochemistry, 1998Co-Authors: Marcelo Hermeslima, Kenneth B StoreyAbstract:Many anurans have excellent Dehydration tolerance that allows endurance of the loss of up to 50-60% of total body water. One of the effects of severe Dehydration is circulatory impairment due the reduced volume and increased viscosity of blood, which leads to organ hypoxia. The rehydration situation, therefore, involves a reoxygenation of tissues that may include elements of oxidative stress that resemble the injury in post-ischemic reperfusion of mammalian organs. The role of endogenous defenses against oxygen radicals in the tolerance of severe Dehydration by leopard frogs, Rana pipiens, was investigated by monitoring the activities of antioxidant enzymes and glutathione levels (reduced GSH and oxidized GSSG) in leg muscle and liver of control, 50%-dehydrated, and fully rehydrated frogs. The maximal activities of muscle catalase and liver glutathione peroxidase, measured per mg soluble protein, increased significantly by 52 and 74%, respectively, after Dehydration whereas muscle superoxide dismutase and glutathione reductase activities responded oppositely, decreasing by 32 and 35%, respectively. Enzyme activities returned to control levels after full rehydration. Hepatic GSH and GSSG increased early in the rehydration process (30% recovery of total body water), but returned to control levels after full recovery. A similar trend was observed for liver GSSG. The elevation of antioxidant defenses against peroxides during Dehydration could provide protection against post-hypoxic oxyradical stress during rehydration. Indeed, analysis of one product of lipid peroxidation, thiobarbituric acid reactive substances, in frog tissues gave no indication of oxidative stress during the Dehydration/rehydration cycle.
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metabolic effects of Dehydration on an aquatic frog rana pipiens
The Journal of Experimental Biology, 1995Co-Authors: Thomas A Churchill, Kenneth B StoreyAbstract:Cellular responses to Dehydration were analyzed in six organs of leopard frogs Rana pipiens. Frogs at 5 degrees C endured the loss of up to 50% of their total body water content but water contents of individual organs were strongly defended. Skeletal muscle water content was strongly affected by Dehydration, dropping from 80.7% of wet mass in controls to 67.2% in frogs that had lost 50% of their total body water. However, water contents of internal organs dropped by only 3-8% of their wet masses. Water contents of all organs except skeletal muscle were fully restored by 24h of rehydration in water at 5 degrees C. Dehydration had no consistent effect on the protein content of five organs but in a sixth, the kidney, protein levels were elevated (by 60-72%) at the higher levels of Dehydration and during rehydration. Dehydration led to a rapid increase in glucose concentration in the liver; compared with control values of 13 +/- 2 nmol mg-1 protein, levels were doubled by 12.2% Dehydration and continued to increase to a maximum of 307 +/- 44 nmol mg-1 protein (20 mumol g-1 wet mass) in 50% dehydrated frogs. Glucose accumulation was supported by a decrease in liver glycogen content and a parallel rise in glucose 6-phosphate levels, but not in the levels of other glycolytic intermediates, confirming that glycogenolytic flux was being directed into glucose synthesis. Blood glucose levels also increased as a function of increasing Dehydration, reaching values 13.8 times higher than controls, but only the kidney and brain showed a significant accumulation of glucose over the course of Dehydration.(ABSTRACT TRUNCATED AT 250 WORDS)
Abou Yobi - One of the best experts on this subject based on the ideXlab platform.
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sporobolus stapfianus insights into desiccation tolerance in the resurrection grasses from linking transcriptomics to metabolomics
BMC Plant Biology, 2017Co-Authors: Abou Yobi, Karen Schlauch, Richard L Tillett, Catherine Espinoza, Bernard W M Wone, John C Cushman, Melvin J OliverAbstract:Understanding the response of resurrection angiosperms to Dehydration and rehydration is critical for deciphering the mechanisms of how plants cope with the rigors of water loss from their vegetative tissues. We have focused our studies on the C4 resurrection grass, Sporobolus stapfianus Gandoger, as a member of a group of important forage grasses. We have combined non-targeted metabolomics with transcriptomics, via a NimbleGen array platform, to develop an understanding of how gene expression and metabolite profiles can be linked to generate a more detailed mechanistic appreciation of the cellular response to both desiccation and rehydration. The rehydration transcriptome and metabolome are primarily geared towards the rapid return of photosynthesis, energy metabolism, protein turnover, and protein synthesis during the rehydration phase. However, there are some metabolites associated with ROS protection that remain elevated during rehydration, most notably the tocopherols. The analysis of the Dehydration transcriptome reveals a strong concordance between transcript abundance and the associated metabolite abundance reported earlier, but only in responses that are directly related to cellular protection during Dehydration: carbohydrate metabolism and redox homeostasis. The transcriptome response also provides strong support for the involvement of cellular protection processes as exemplified by the increases in the abundance of transcripts encoding late embryogenesis abundant (LEA) proteins, anti-oxidant enzymes, early light-induced proteins (ELIP) proteins, and cell-wall modification enzymes. There is little concordance between transcript and metabolite abundance for processes such as amino acid metabolism that do not appear to contribute directly to cellular protection, but are nonetheless important for the desiccation tolerant phenotype of S. stapfianus. The transcriptomes of both Dehydration and rehydration offer insight into the complexity of the regulation of responses to these processes that involve complex signaling pathways and associated transcription factors. ABA appears to be important in the control of gene expression in both the latter stages of the Dehydration and the early stages of rehydration. These findings add to the growing body of information detailing how plants tolerate and survive the severe cellular perturbations of Dehydration, desiccation, and rehydration.
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sporobolus stapfianus insights into desiccation tolerance in the resurrection grasses from linking transcriptomics to metabolomics
BMC Plant Biology, 2017Co-Authors: Abou Yobi, Karen Schlauch, Richard L Tillett, Catherine Espinoza, Bernard W M Wone, John C Cushman, Melvin J OliverAbstract:Understanding the response of resurrection angiosperms to Dehydration and rehydration is critical for deciphering the mechanisms of how plants cope with the rigors of water loss from their vegetative tissues. We have focused our studies on the C4 resurrection grass, Sporobolus stapfianus Gandoger, as a member of a group of important forage grasses. We have combined non-targeted metabolomics with transcriptomics, via a NimbleGen array platform, to develop an understanding of how gene expression and metabolite profiles can be linked to generate a more detailed mechanistic appreciation of the cellular response to both desiccation and rehydration. The rehydration transcriptome and metabolome are primarily geared towards the rapid return of photosynthesis, energy metabolism, protein turnover, and protein synthesis during the rehydration phase. However, there are some metabolites associated with ROS protection that remain elevated during rehydration, most notably the tocopherols. The analysis of the Dehydration transcriptome reveals a strong concordance between transcript abundance and the associated metabolite abundance reported earlier, but only in responses that are directly related to cellular protection during Dehydration: carbohydrate metabolism and redox homeostasis. The transcriptome response also provides strong support for the involvement of cellular protection processes as exemplified by the increases in the abundance of transcripts encoding late embryogenesis abundant (LEA) proteins, anti-oxidant enzymes, early light-induced proteins (ELIP) proteins, and cell-wall modification enzymes. There is little concordance between transcript and metabolite abundance for processes such as amino acid metabolism that do not appear to contribute directly to cellular protection, but are nonetheless important for the desiccation tolerant phenotype of S. stapfianus. The transcriptomes of both Dehydration and rehydration offer insight into the complexity of the regulation of responses to these processes that involve complex signaling pathways and associated transcription factors. ABA appears to be important in the control of gene expression in both the latter stages of the Dehydration and the early stages of rehydration. These findings add to the growing body of information detailing how plants tolerate and survive the severe cellular perturbations of Dehydration, desiccation, and rehydration.
Freedom Tshabuse - One of the best experts on this subject based on the ideXlab platform.
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glycerolipid analysis during desiccation and recovery of the resurrection plant xerophyta humilis bak dur and schinz
Plant Cell and Environment, 2018Co-Authors: Freedom Tshabuse, Lydie Humbert, Deborah Moura, Dominique Rainteau, Christophe Espinasse, Sébastien Acket, Franck Merlier, J. Mark Farrant, Abdelghani Idrissi, Mohamad Suhail RafudeenAbstract:: Xerophyta humilis is a poikilochlorophyllous monocot resurrection plant used as a model to study vegetative desiccation tolerance. Dehydration imposes tension and ultimate loss of integrity of membranes in desiccation sensitive species. We investigated the predominant molecular species of glycerolipids present in root and leaf tissues, using multiple reaction monitoring mass spectrometry, and then analysed changes therein during Dehydration and subsequent rehydration of whole plants. The presence of fatty acids with long carbon chains and with odd numbers of carbons were detected and confirmed by gas chromatography. Dehydration of both leaves and roots resulted in an increase in species containing polyunsaturated fatty acids and a decrease in disaturated species. Upon rehydration, lipid saturation was reversed, with this being initiated immediately upon watering in roots but only 12-24 hr later in leaves. Relative levels of species with short-chained odd-numbered saturated fatty acids decreased during Dehydration and increased during rehydration, whereas the reverse trend was observed for long-chained fatty acids. X. humilis has a unique lipid composition, this report being one of the few to demonstrate the presence of odd-numbered fatty acids in plant phosphoglycerolipids.
