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Adolf Nahrstedt - One of the best experts on this subject based on the ideXlab platform.

  • quantitative determination of curcuminoids in curcuma rhizomes and rapid differentiation of curcuma domestica val and curcuma xanthorrhiza roxb by capillary electrophoresis
    Phytochemical Analysis, 2004
    Co-Authors: Matthias Lechtenberg, B Quandt, Adolf Nahrstedt
    Abstract:

    The three major curcuminoids, curcumin, Demethoxycurcumin and bis-Demethoxycurcumin, from Curcuma domestica Val. (Curcuma longa L.) and Curcuma xanthorrhiza Roxb. (Zingiberaceae) were fully separated and quantified in less than 5 min using a capillary zone electrophoresis method with standard fused-silica capillaries and photodiode array detection. An electrolyte solution of 20 mM phosphate, 50 mM sodium hydroxide and 14 mM beta-cyclodextrin was found to be appropriate. Quantification was performed with 3,4-dimethoxy-trans-cinnamic acid as internal standard, and the limit of detection was 0.01 mg/mL. Extraction, stabilisation during sample storage and quantification procedures were optimised and carried out with drugs and commercial curry powder from different provenances. The results were compared with the photometric method of the monograph Curcumae xanthorrhizae rhizoma of the European Pharmacopoeia.

Soyoung Park - One of the best experts on this subject based on the ideXlab platform.

  • curcuminoids from curcuma longa l zingiberaceae that protect pc12 rat pheochromocytoma and normal human umbilical vein endothelial cells from βa 1 42 insult
    Neuroscience Letters, 2001
    Co-Authors: Soyoung Park
    Abstract:

    β-Amyloid (βA) induced oxidative stress is a well-established pathway of neuronal cell death in Alzheimer's disease. From turmeric, Curcuma longa L. (Zingiberaceae), three curcuminoids, curcumin, Demethoxycurcumin, and bisDemethoxycurcumin, were found to protect PC12 rat pheochromocytoma and normal human umbilical vein endothelial (HUVEC) cells from βA(1–42) insult, as measured by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide reduction assay. ED50 values of curcumin, Demethoxycurcumin, and bisDemethoxycurcumin toward PC12 and HUVEC cells were 7.1±0.3, 4.7±0.1, 3.5±0.2 μg/ml and 6.8±0.4, 4.2±0.3, and 3.0±0.3 μg/ml, respectively. These compounds were better antioxidants than α-tocopherol as determined by DPPH radical trapping experiment. α-Tocopherol did not protect the cells from βA(1–42) insult even at>50 μg/ml concentration. The results suggest that these compounds may be protecting the cells from βA(1–42) insult through antioxidant pathway.

  • curcuminoids from curcuma longa l zingiberaceae that protect pc12 rat pheochromocytoma and normal human umbilical vein endothelial cells from βa 1 42 insult
    Neuroscience Letters, 2001
    Co-Authors: Darrick S H L Kim, Soyoung Park, Jinyung Kim
    Abstract:

    beta-Amyloid (betaA) induced oxidative stress is a well-established pathway of neuronal cell death in Alzheimer's disease. From turmeric, Curcuma longa L. (Zingiberaceae), three curcuminoids, curcumin, Demethoxycurcumin, and bisDemethoxycurcumin, were found to protect PC12 rat pheochromocytoma and normal human umbilical vein endothelial (HUVEC) cells from betaA(1-42) insult, as measured by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide reduction assay. ED(50) values of curcumin, Demethoxycurcumin, and bisDemethoxycurcumin toward PC12 and HUVEC cells were 7.1+/-0.3, 4.7+/-0.1, 3.5+/-0.2 microg/ml and 6.8+/-0.4, 4.2+/-0.3, and 3.0+/-0.3 microg/ml, respectively. These compounds were better antioxidants than alpha-tocopherol as determined by DPPH radical trapping experiment. alpha-Tocopherol did not protect the cells from betaA(1-42) insult even at>50 microg/ml concentration. The results suggest that these compounds may be protecting the cells from betaA(1-42) insult through antioxidant pathway.

Matthias Lechtenberg - One of the best experts on this subject based on the ideXlab platform.

  • quantitative determination of curcuminoids in curcuma rhizomes and rapid differentiation of curcuma domestica val and curcuma xanthorrhiza roxb by capillary electrophoresis
    Phytochemical Analysis, 2004
    Co-Authors: Matthias Lechtenberg, B Quandt, Adolf Nahrstedt
    Abstract:

    The three major curcuminoids, curcumin, Demethoxycurcumin and bis-Demethoxycurcumin, from Curcuma domestica Val. (Curcuma longa L.) and Curcuma xanthorrhiza Roxb. (Zingiberaceae) were fully separated and quantified in less than 5 min using a capillary zone electrophoresis method with standard fused-silica capillaries and photodiode array detection. An electrolyte solution of 20 mM phosphate, 50 mM sodium hydroxide and 14 mM beta-cyclodextrin was found to be appropriate. Quantification was performed with 3,4-dimethoxy-trans-cinnamic acid as internal standard, and the limit of detection was 0.01 mg/mL. Extraction, stabilisation during sample storage and quantification procedures were optimised and carried out with drugs and commercial curry powder from different provenances. The results were compared with the photometric method of the monograph Curcumae xanthorrhizae rhizoma of the European Pharmacopoeia.

Jinyung Kim - One of the best experts on this subject based on the ideXlab platform.

  • curcuminoids from curcuma longa l zingiberaceae that protect pc12 rat pheochromocytoma and normal human umbilical vein endothelial cells from βa 1 42 insult
    Neuroscience Letters, 2001
    Co-Authors: Darrick S H L Kim, Soyoung Park, Jinyung Kim
    Abstract:

    beta-Amyloid (betaA) induced oxidative stress is a well-established pathway of neuronal cell death in Alzheimer's disease. From turmeric, Curcuma longa L. (Zingiberaceae), three curcuminoids, curcumin, Demethoxycurcumin, and bisDemethoxycurcumin, were found to protect PC12 rat pheochromocytoma and normal human umbilical vein endothelial (HUVEC) cells from betaA(1-42) insult, as measured by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide reduction assay. ED(50) values of curcumin, Demethoxycurcumin, and bisDemethoxycurcumin toward PC12 and HUVEC cells were 7.1+/-0.3, 4.7+/-0.1, 3.5+/-0.2 microg/ml and 6.8+/-0.4, 4.2+/-0.3, and 3.0+/-0.3 microg/ml, respectively. These compounds were better antioxidants than alpha-tocopherol as determined by DPPH radical trapping experiment. alpha-Tocopherol did not protect the cells from betaA(1-42) insult even at>50 microg/ml concentration. The results suggest that these compounds may be protecting the cells from betaA(1-42) insult through antioxidant pathway.

Yosi Bayu Murti - One of the best experts on this subject based on the ideXlab platform.

  • identifikasi senyawa curcumin bisDemethoxycurcumin dan Demethoxycurcumin rimpang curcuma xanthorrhiza roxb dan curcuma longa l dengan 1h nmr
    Proceeding Biology Education Conference: Biology Science Enviromental and Learning, 2018
    Co-Authors: Erwin Nur Indiarto, Tri Rini Nuringtyas, Yekti Asih Purwestri, Respati Tri Swasono, Yosi Bayu Murti
    Abstract:

    Curcumin and its derivates ( BisDemethoxycurcumin, Demethoxycurcumin ) are natural dyes found in Curcuma xanthorrhiza dan Curcuma longa . Parmacologic evidence suggests all three are antioxidant, antiinflamatory, and anticancer. The objective of this research was to identify and measure the intensity of Curcumin compounds, BisDemethoxycurcumin , and Demethoxycurcumin rhizome C. xanthorrhiza and C. longa by using 1 H NMR spectra. Rhizome C.xanthorrhiza and C. longa collected from Wonogiri, Central Java. The rhizomes were dried in the oven at 40 °C and were powdered using a blender. The dried powder sample was extracted using methanol- d 4  containing 0.01% TMSP and followed by ultrasonication for 15 minutes. The supernatant was separated from the solution by 10.000 rpm centrifugation for 10 minutes. The supernatant was transferred to NMR tube and analysed by using JEOL 500 MHz NMR. The obtained data were analysed by Mnova 12 software and Simca 14. Results of comparsion of 1 H NMR spectra of both rhizomes with reference were identified Curcumin, BisDemethoxycurcumin, Demethoxycurcumin compounds. The highest intensity all of three compound was found in Curcuma longa and with One way Anova test result obtained p<0,05. The two rhizomes show a good separation on the OPLS-DA scoreplot with Q2 82,1% indicating a good model. The study showed that Curcumin, BisDemethoxycurcumin, Demethoxycurcumin compounds were found in both rhizomes and had different intensity

  • Identifikasi Senyawa Curcumin, BisDemethoxycurcumin dan Demethoxycurcumin Rimpang Curcuma xanthorrhiza Roxb. dan Curcuma longa L. dengan 1H NMR
    2018
    Co-Authors: Erwin Nur Indiarto, Tri Rini Nuringtyas, Yekti Asih Purwestri, Respati Tri Swasono, Yosi Bayu Murti
    Abstract:

    Curcumin and its derivates ( BisDemethoxycurcumin, Demethoxycurcumin ) are natural dyes found in Curcuma xanthorrhiza dan Curcuma longa . Parmacologic evidence suggests all three are antioxidant, antiinflamatory, and anticancer. The objective of this research was to identify and measure the intensity of Curcumin compounds, BisDemethoxycurcumin , and Demethoxycurcumin rhizome C. xanthorrhiza and C. longa by using 1 H NMR spectra. Rhizome C.xanthorrhiza and C. longa collected from Wonogiri, Central Java. The rhizomes were dried in the oven at 40 °C and were powdered using a blender. The dried powder sample was extracted using methanol- d 4  containing 0.01% TMSP and followed by ultrasonication for 15 minutes. The supernatant was separated from the solution by 10.000 rpm centrifugation for 10 minutes. The supernatant was transferred to NMR tube and analysed by using JEOL 500 MHz NMR. The obtained data were analysed by Mnova 12 software and Simca 14. Results of comparsion of 1 H NMR spectra of both rhizomes with reference were identified Curcumin, BisDemethoxycurcumin, Demethoxycurcumin compounds. The highest intensity all of three compound was found in Curcuma longa and with One way Anova test result obtained p