The Experts below are selected from a list of 261 Experts worldwide ranked by ideXlab platform
Steven L Kelly - One of the best experts on this subject based on the ideXlab platform.
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use of 70 Alcohol for the routine removal of microbial hard surface bioburden in life science cleanrooms
Future Microbiology, 2014Co-Authors: Richard Salvage, Claire M Hull, Diane E Kelly, Steven L KellyAbstract:ABSTRACT Alcohol-based disinfectants are used for the removal of microbial hard surface bioburden in Life science Cleanrooms. Evidence for using formulations containing 70% Alcohol has been lost over time but probably originates from historical observations of the activity of 60–70% Alcohol. Tradition is no longer adequate to inform contemporary cleaning practice. We evaluated the efficacy of ethanol, isopropanol and trade-specific Denatured Alcohol 7 against vegetative Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli and Enterococcus hirae using standardized European Suspension and Hard Surface tests. All three Alcohols were effective at lower concentrations than the 70% standard. This constitutes the first evaluation of disinfectant formulations containing ≤70% Alcohol using standard methodology. The utility of trade-specific Denatured Alcohol #7 and evidence-based cleanroom practice warrant further validation.
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Use of 70% Alcohol for the routine removal of microbial hard surface bioburden in life science cleanrooms
Future Microbiology, 2014Co-Authors: Richard Salvage, Claire M Hull, Diane E Kelly, Steven L KellyAbstract:Alcohol-based disinfectants are used for the removal of microbial hard surface bioburden in Life science Cleanrooms. Evidence for using formulations containing 70% Alcohol has been lost over time but probably originates from historical observations of the activity of 60-70% Alcohol. Tradition is no longer adequate to inform contemporary cleaning practice. We evaluated the efficacy of ethanol, isopropanol and trade-specific Denatured Alcohol 7 against vegetative Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli and Enterococcus hirae using standardized European Suspension and Hard Surface tests. All three Alcohols were effective at lower concentrations than the 70% standard. This constitutes the first evaluation of disinfectant formulations containing ≤70% Alcohol using standard methodology. The utility of trade-specific Denatured Alcohol #7 and evidence-based cleanroom practice warrant further validation.
Andre H. Lawrence - One of the best experts on this subject based on the ideXlab platform.
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Direct Injection Gas Chromatographic/Mass Spectrometric Analysis for Denatonium Benzoate in Specific Denatured Alcohol Formulations
Analytical Chemistry, 1998Co-Authors: Michel Hupé, Jean Harnois, Andre H. LawrenceAbstract:Direct injection GC/MS was investigated for the analysis of benzyldiethyl(2,6-xylylcarbamoylmethyl)ammonium benzoate (Bitrex), a quaternary ammonium salt, in various Canadian Denatured Alcohol formulations. Bitrex yielded predominantly a peak due to the neutral diethylamine derivative (I). The structure of I, elucidated by MS and NMR, is strongly related to that of the cation of Bitrex. Compound I was formed from Bitrex in the heated injector port of the GC via a decomposition reaction similar to Stevens rearrangement. The response of I was found to be dependent on the injector port temperature, and the optimal temperature was determined to be in the range 250−350 °C. The GC/MS response of I in SIM mode was used to quantify Bitrex. The effects of the codenaturants sucrose octaacetate (SOA), diethyl phthalate (DEP), and camphor, which are present at much higher concentration than Bitrex in several formulations, were also investigated. The presence of SOA enhanced the response of the analyte considerably, w...
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direct injection gas chromatographic mass spectrometric analysis for denatonium benzoate in specific Denatured Alcohol formulations
Analytical Chemistry, 1998Co-Authors: Michel Hupé, Jean Harnois, Andre H. LawrenceAbstract:Direct injection GC/MS was investigated for the analysis of benzyldiethyl(2,6-xylylcarbamoylmethyl)ammonium benzoate (Bitrex), a quaternary ammonium salt, in various Canadian Denatured Alcohol formulations. Bitrex yielded predominantly a peak due to the neutral diethylamine derivative (I). The structure of I, elucidated by MS and NMR, is strongly related to that of the cation of Bitrex. Compound I was formed from Bitrex in the heated injector port of the GC via a decomposition reaction similar to Stevens rearrangement. The response of I was found to be dependent on the injector port temperature, and the optimal temperature was determined to be in the range 250−350 °C. The GC/MS response of I in SIM mode was used to quantify Bitrex. The effects of the codenaturants sucrose octaacetate (SOA), diethyl phthalate (DEP), and camphor, which are present at much higher concentration than Bitrex in several formulations, were also investigated. The presence of SOA enhanced the response of the analyte considerably, w...
Niall Eames - One of the best experts on this subject based on the ideXlab platform.
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Antisepsis of the skin before spinal surgery with povidone iodine-Alcohol followed by chlorhexidine gluconate-Alcohol versus povidone iodine-Alcohol applied twice for the prevention of contamination of the wound by bacteria: a randomised controlled t
Journal of Bone and Joint Surgery-british Volume, 2017Co-Authors: Sheila Patrick, Andrew Mcdowell, Andrew W. Lee, Alessandra Frau, Una Martin, G. C. Mclorinan, Evelyn Gardner, Niall EamesAbstract:Aims The aim of this study was to determine whether the sequential application of povidone iodine-Alcohol (PVI) followed by chlorhexidine gluconate-Alcohol (CHG) would reduce surgical wound contamination to a greater extent than PVI applied twice in patients undergoing spinal surgery. Patients and Methods A single-centre, interventional, two arm, parallel group randomised controlled trial was undertaken, involving 407 patients who underwent elective spinal surgery. For 203 patients, the skin was disinfected before surgery using PVI (10% [w/w (1% w/w available iodine)] in 95% industrial Denatured Alcohol, povidone iodine; Videne Alcoholic Tincture) twice, and for 204 patients using PVI once followed by CHG (2% [w/v] chlorhexidine gluconate in 70% [v/v] isopropyl Alcohol; Chloraprep with tint). The primary outcome measure was contamination of the wound determined by aerobic and anaerobic bacterial growth from samples taken after disinfection. Results The detection of viable bacteria in any one of the samples taken after disinfection (culture-positive) was significantly lower in the group treated with both PVI and CHG than in the group treated with PVI alone (59 (29.1%) versus 85 (41.7%), p = 0.009; odds ratio 0.574; 95% confidence interval, 0.380 to 0.866). Conclusions Antisepsis of the skin with the sequential application of PVI and CHG more effectively reduces the contamination of a surgical wound than PVI alone. Cite this article: Bone Joint J 2017;99-B:1354–65.
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Pre-spinal surgery skin antisepsis with povidone iodine-Alcohol followed by chlorhexidine gluconate-Alcohol versus povidone iodine-Alcohol applied twice for prevention of surgical wound contamination by bacteria: a randomised, controlled trial.
Journal of Bone and Joint Surgery-british Volume, 2017Co-Authors: Sheila Patrick, Andrew Mcdowell, Andrew W. Lee, Alessandra Frau, Una Martin, Evie Gardiner, G. C. Mclorinan, Niall EamesAbstract:Aims:To determine if sequential application of povidone iodine-Alcohol (PVI) followed by chlorhexidine gluconate-Alcohol (CHG) would reduce surgical wound contamination to a greater extent than PVI applied twice in spinal surgery patients. Patients and Methods: A single-centre, interventional, two arm, parallel group randomised controlled trial of 407 patients attending hospital for elective spinal surgery was conducted. For 203 patients, pre-surgical skin disinfection was by application of PVI (10% [w/w (1% w/w available iodine)] povidone iodine in 95% industrial Denatured Alcohol; Videne Alcoholic Tincture) twice and for 204 patients application of PVI once followed by application of CHG (2% [w/v] chlorhexadine gluconate in 70% [v/v] isopropyl Alcohol; Chloraprep with tint). The primary outcome measure was post-skindisinfection surgical site contamination determined by aerobic and anaerobicbacterial growth from post-skin disinfection samples. Results: The detection of viable bacteria in any one of the post-skin disinfection samples (culture-positive) was significantly lower in the group treated with both PVI and CHG than in the group treated with PVI alone: 29.1% (59) vs 41.7% (85), P=0.009; relative risk, 0.574; 95% confidence interval, 0.380 to 0.866. Conclusions: Skin antisepsis with sequential application of PVI and CHG more effectively reduces surgical wound contamination than PVI alone.
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Antisepsis of the skin before spinal surgery with povidone iodine-Alcohol followed by chlorhexidine gluconate-Alcohol versus povidone iodine-Alcohol applied twice for the prevention of contamination of the wound by bacteria: a randomised controlled t
The bone & joint journal, 2017Co-Authors: Sheila Patrick, Andrew Mcdowell, Alessandra Frau, Una Martin, G. C. Mclorinan, Evelyn Gardner, A Lee, Niall EamesAbstract:The aim of this study was to determine whether the sequential application of povidone iodine-Alcohol (PVI) followed by chlorhexidine gluconate-Alcohol (CHG) would reduce surgical wound contamination to a greater extent than PVI applied twice in patients undergoing spinal surgery. A single-centre, interventional, two arm, parallel group randomised controlled trial was undertaken, involving 407 patients who underwent elective spinal surgery. For 203 patients, the skin was disinfected before surgery using PVI (10% [w/w (1% w/w available iodine)] in 95% industrial Denatured Alcohol, povidone iodine; Videne Alcoholic Tincture) twice, and for 204 patients using PVI once followed by CHG (2% [w/v] chlorhexidine gluconate in 70% [v/v] isopropyl Alcohol; Chloraprep with tint). The primary outcome measure was contamination of the wound determined by aerobic and anaerobic bacterial growth from samples taken after disinfection. The detection of viable bacteria in any one of the samples taken after disinfection (culture-positive) was significantly lower in the group treated with both PVI and CHG than in the group treated with PVI alone (59 (29.1%) versus 85 (41.7%), p = 0.009; odds ratio 0.574; 95% confidence interval, 0.380 to 0.866). Antisepsis of the skin with the sequential application of PVI and CHG more effectively reduces the contamination of a surgical wound than PVI alone. Cite this article: Bone Joint J 2017;99-B:1354-65. ©2017 The British Editorial Society of Bone & Joint Surgery.
Richard Salvage - One of the best experts on this subject based on the ideXlab platform.
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use of 70 Alcohol for the routine removal of microbial hard surface bioburden in life science cleanrooms
Future Microbiology, 2014Co-Authors: Richard Salvage, Claire M Hull, Diane E Kelly, Steven L KellyAbstract:ABSTRACT Alcohol-based disinfectants are used for the removal of microbial hard surface bioburden in Life science Cleanrooms. Evidence for using formulations containing 70% Alcohol has been lost over time but probably originates from historical observations of the activity of 60–70% Alcohol. Tradition is no longer adequate to inform contemporary cleaning practice. We evaluated the efficacy of ethanol, isopropanol and trade-specific Denatured Alcohol 7 against vegetative Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli and Enterococcus hirae using standardized European Suspension and Hard Surface tests. All three Alcohols were effective at lower concentrations than the 70% standard. This constitutes the first evaluation of disinfectant formulations containing ≤70% Alcohol using standard methodology. The utility of trade-specific Denatured Alcohol #7 and evidence-based cleanroom practice warrant further validation.
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Use of 70% Alcohol for the routine removal of microbial hard surface bioburden in life science cleanrooms
Future Microbiology, 2014Co-Authors: Richard Salvage, Claire M Hull, Diane E Kelly, Steven L KellyAbstract:Alcohol-based disinfectants are used for the removal of microbial hard surface bioburden in Life science Cleanrooms. Evidence for using formulations containing 70% Alcohol has been lost over time but probably originates from historical observations of the activity of 60-70% Alcohol. Tradition is no longer adequate to inform contemporary cleaning practice. We evaluated the efficacy of ethanol, isopropanol and trade-specific Denatured Alcohol 7 against vegetative Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli and Enterococcus hirae using standardized European Suspension and Hard Surface tests. All three Alcohols were effective at lower concentrations than the 70% standard. This constitutes the first evaluation of disinfectant formulations containing ≤70% Alcohol using standard methodology. The utility of trade-specific Denatured Alcohol #7 and evidence-based cleanroom practice warrant further validation.
Michel Hupé - One of the best experts on this subject based on the ideXlab platform.
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Direct Injection Gas Chromatographic/Mass Spectrometric Analysis for Denatonium Benzoate in Specific Denatured Alcohol Formulations
Analytical Chemistry, 1998Co-Authors: Michel Hupé, Jean Harnois, Andre H. LawrenceAbstract:Direct injection GC/MS was investigated for the analysis of benzyldiethyl(2,6-xylylcarbamoylmethyl)ammonium benzoate (Bitrex), a quaternary ammonium salt, in various Canadian Denatured Alcohol formulations. Bitrex yielded predominantly a peak due to the neutral diethylamine derivative (I). The structure of I, elucidated by MS and NMR, is strongly related to that of the cation of Bitrex. Compound I was formed from Bitrex in the heated injector port of the GC via a decomposition reaction similar to Stevens rearrangement. The response of I was found to be dependent on the injector port temperature, and the optimal temperature was determined to be in the range 250−350 °C. The GC/MS response of I in SIM mode was used to quantify Bitrex. The effects of the codenaturants sucrose octaacetate (SOA), diethyl phthalate (DEP), and camphor, which are present at much higher concentration than Bitrex in several formulations, were also investigated. The presence of SOA enhanced the response of the analyte considerably, w...
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direct injection gas chromatographic mass spectrometric analysis for denatonium benzoate in specific Denatured Alcohol formulations
Analytical Chemistry, 1998Co-Authors: Michel Hupé, Jean Harnois, Andre H. LawrenceAbstract:Direct injection GC/MS was investigated for the analysis of benzyldiethyl(2,6-xylylcarbamoylmethyl)ammonium benzoate (Bitrex), a quaternary ammonium salt, in various Canadian Denatured Alcohol formulations. Bitrex yielded predominantly a peak due to the neutral diethylamine derivative (I). The structure of I, elucidated by MS and NMR, is strongly related to that of the cation of Bitrex. Compound I was formed from Bitrex in the heated injector port of the GC via a decomposition reaction similar to Stevens rearrangement. The response of I was found to be dependent on the injector port temperature, and the optimal temperature was determined to be in the range 250−350 °C. The GC/MS response of I in SIM mode was used to quantify Bitrex. The effects of the codenaturants sucrose octaacetate (SOA), diethyl phthalate (DEP), and camphor, which are present at much higher concentration than Bitrex in several formulations, were also investigated. The presence of SOA enhanced the response of the analyte considerably, w...