The Experts below are selected from a list of 309 Experts worldwide ranked by ideXlab platform
P Valachovic - One of the best experts on this subject based on the ideXlab platform.
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ultrasound assisted extraction of water soluble polysaccharides from the roots of valerian valeriana officinalis l
Ultrasonics Sonochemistry, 2002Co-Authors: Zdenka Hromadkova, Anna Ebringerova, P ValachovicAbstract:Abstract The insoluble plant residues, obtained after preparation of medicinal Tinctures from the roots of valerian (Valeriana officinalis L.) by classical and ultrasound-assisted extraction with aqueous ethanol in a pilot plant, were subsequently treated with hot water to isolate the accessible polysaccharide cell wall components. At almost equal amounts of the hot-water extractable material, the yields of the recovered polysaccharides were lower in the ultrasonical experiment. This is due to the fact that a part of accessible polysaccharides were already solubilised by the aqueous ethanol and recoverable from the medicinal Tincture. Therefore, the net yield of extracted polysaccharides was enhanced in the ultrasonical procedure. This fact as well as the sugar composition and structural features of the isolated polysaccharides suggest that ultrasonication have attacked the integrity of cell walls, released and degraded its most accessible polysaccharides (pectic polysaccharides and starch) and increased also the extractibility of its less accessible components – xylan, mannan and glucan. The water-soluble polysaccharide fractions from both the conventional and ultrasonical experiments exhibit significant immunostimulatory activities in mitogenic and comitogenic thymocyte tests.
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towards the industrial production of medicinal Tincture by ultrasound assisted extraction
Ultrasonics Sonochemistry, 2001Co-Authors: P Valachovic, A Pechova, Timothy J MasonAbstract:Experiments related to the industrial production of medicinal Tinctures of sage (Salvia officinalis L.) and valerian (Valeriana officinalis L.) were performed using ultrasonically assisted extraction and quality of the final Tincture was evaluated by gas chromatography and dry residue determination. The constituents of the active sage compound: borneol, cineole, α- and β-thujone and also the dry residue were measured. In the case of valerian extractions, the efficiency of sonicated extraction was compared with classical extraction. The course of only the dry residue was monitored. The influence of ultrasound on the quality of valerian Tincture was examined by HPLC.
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comparison of classical and ultrasound assisted extraction of polysaccharides from salvia officinalis l
Ultrasonics Sonochemistry, 1999Co-Authors: Zdenka Hromadkova, Anna Ebringerova, P ValachovicAbstract:After preparation of medicine Tinctures from the herbal plant Salvia officinalis by classical and ultrasound-assisted extraction with aqueous ethanol, the insoluble plant residues were subsequently treated with hot water and dilute alkali to isolate polysaccharide cell wall components. The yields of the hot water extract as well as total extracted polysaccharides were higher in the case of the ultrasound-treated plant in both laboratory and pilot plant experiments. The water-extractable polysaccharide fractions, in all cases, contained glucose, galactose and arabinose as main sugar components, whereas the alkali-extractable fractions were rich in xylans. The fractions also contained considerable amounts of proteins. The water-soluble polysaccharides may contribute to the biological activity of the plant decoction. The results indicate that the addition of a subsequent extraction step during the preparation of the herbal Tincture might contribute to a better exploitation of the raw material.
Guenter Gisselmann - One of the best experts on this subject based on the ideXlab platform.
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identification of glycyrrhiza as the rikkunshito constituent with the highest antagonistic potential on heterologously expressed 5 ht3a receptors due to the action of flavonoids
Frontiers in Pharmacology, 2015Co-Authors: Robin Herbrechter, Paul M Ziemba, Katrin M Hoffmann, Hanns Hatt, Markus Werner, Guenter GisselmannAbstract:The traditional Japanese phytomedicine rikkunshito is traditionally used for the treatment of gastrointestinal motility disorders, cachexia and nausea. These effects indicate 5-HT3 receptor antagonism, due to the involvement of these receptors in such pathophysiological processes. E.g., setrons, specific 5-HT3 receptor antagonists are the strongest antiemetics, developed so far. Therefore, the antagonistic effects of the eight rikkunshito constituents at heterologously expressed 5-HT3Areceptors were analyzed using the two-electrode voltage-clamp technique. The results indicate that Tinctures from Aurantii, Ginseng, Zingiberis, Atractylodis and Glycyrrhiza inhibited the 5-HT3A receptor response, whereas the Tinctures of Poria cocos, Jujubae and Pinellia exhibited no effect. Surprisingly, the strongest antagonism was found for Glycyrrhiza, whereas the Zingiberis Tincture, which is considered to be primarily responsible for the effect of rikkunshito, exhibited the weakest antagonism of 5-HT3A receptors. Rikkunshito contains various vanilloids, ginsenosides and flavonoids, a portion of which show an antagonistic effect on 5-HT3 receptors. A screening of the established ingredients of the active rikkunshito constituents and related substances lead to the identification of new antagonists within the class of flavonoids. The flavonoids (-)-liquiritigenin, glabridin and licochalcone A from Glycyrrhiza species were found to be the most effective inhibitors of the 5-HT-induced currents in the screening. The flavonoids (-)-liquiritigenin and hesperetin from Aurantii inhibited the receptor response in a non-competitive manner, whereas glabridin and licochalcone A exhibited a potential competitive antagonism. Furthermore, licochalcone A acts as a partial antagonist of 5-HT3A receptors. Thus, this study reveals new 5-HT3A receptor antagonists with the aid of increasing the comprehension of the complex effects of rikkunshito.
Robin Herbrechter - One of the best experts on this subject based on the ideXlab platform.
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identification of glycyrrhiza as the rikkunshito constituent with the highest antagonistic potential on heterologously expressed 5 ht3a receptors due to the action of flavonoids
Frontiers in Pharmacology, 2015Co-Authors: Robin Herbrechter, Paul M Ziemba, Katrin M Hoffmann, Hanns Hatt, Markus Werner, Guenter GisselmannAbstract:The traditional Japanese phytomedicine rikkunshito is traditionally used for the treatment of gastrointestinal motility disorders, cachexia and nausea. These effects indicate 5-HT3 receptor antagonism, due to the involvement of these receptors in such pathophysiological processes. E.g., setrons, specific 5-HT3 receptor antagonists are the strongest antiemetics, developed so far. Therefore, the antagonistic effects of the eight rikkunshito constituents at heterologously expressed 5-HT3Areceptors were analyzed using the two-electrode voltage-clamp technique. The results indicate that Tinctures from Aurantii, Ginseng, Zingiberis, Atractylodis and Glycyrrhiza inhibited the 5-HT3A receptor response, whereas the Tinctures of Poria cocos, Jujubae and Pinellia exhibited no effect. Surprisingly, the strongest antagonism was found for Glycyrrhiza, whereas the Zingiberis Tincture, which is considered to be primarily responsible for the effect of rikkunshito, exhibited the weakest antagonism of 5-HT3A receptors. Rikkunshito contains various vanilloids, ginsenosides and flavonoids, a portion of which show an antagonistic effect on 5-HT3 receptors. A screening of the established ingredients of the active rikkunshito constituents and related substances lead to the identification of new antagonists within the class of flavonoids. The flavonoids (-)-liquiritigenin, glabridin and licochalcone A from Glycyrrhiza species were found to be the most effective inhibitors of the 5-HT-induced currents in the screening. The flavonoids (-)-liquiritigenin and hesperetin from Aurantii inhibited the receptor response in a non-competitive manner, whereas glabridin and licochalcone A exhibited a potential competitive antagonism. Furthermore, licochalcone A acts as a partial antagonist of 5-HT3A receptors. Thus, this study reveals new 5-HT3A receptor antagonists with the aid of increasing the comprehension of the complex effects of rikkunshito.
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Identification of \(\it Glycyrrhiza\) as the rikkunshito constituent with the highest antagonistic potential on heterologously expressed \(5-HT_{3A}\) receptors due to the action of flavonoids
2015Co-Authors: Robin Herbrechter, Ziemba, Paul Michael, Hoffmann, Katrin M., Hatt Hanns, Werner Markus, Gisselmann GünterAbstract:The traditional Japanese phytomedicine rikkunshito is traditionally used for the treatment of gastrointestinal motility disorders, cachexia and nausea. These effects indicate \(5-HT_{3}\) receptor antagonism, due to the involvement of these receptors in such pathophysiological processes. E.g., setrons, specific \(5-HT_{3}\) receptor antagonists are the strongest antiemetics, developed so far. Therefore, the antagonistic effects of the eight rikkunshito constituents at heterologously expressed \(5-HT_{3A}\)receptors were analyzed using the two-electrode voltage-clamp technique. The results indicate that Tinctures from \(\textit {Aurantii, Ginseng, Zingiberis, Atractylodis}\) and \(\it Glycyrrhiza\) inhibited the \(5-HT_{3A}\) receptor response, whereas the Tinctures of \(\textit {Poria cocos, Jujubae}\) and \(\it Pinellia\) exhibited no effect. Surprisingly, the strongest antagonism was found for \(\it Glycyrrhiza\), whereas the \(\it Zingiberis\) Tincture, which is considered to be primarily responsible for the effect of rikkunshito, exhibited the weakest antagonism of \(5-HT_{3A}\) receptors. Rikkunshito contains various vanilloids, ginsenosides and flavonoids, a portion of which show an antagonistic effect on \(5-HT_{3}\) receptors. A screening of the established ingredients of the active rikkunshito constituents and related substances lead to the identification of new antagonists within the class of flavonoids. The flavonoids (-)-liquiritigenin, glabridin and licochalcone A from \(\it Glycyrrhiza\) species were found to be the most effective inhibitors of the 5-HT-induced currents in the screening. The flavonoids (-)-liquiritigenin and hesperetin from \(\it Aurantii\) inhibited the receptor response in a non-competitive manner, whereas glabridin and licochalcone A exhibited a potential competitive antagonism. Furthermore, licochalcone A acts as a partial antagonist of \(5-HT_{3A}\) receptors. Thus, this study reveals new \(5-HT_{3A}\) receptor antagonists with the aid of increasing the comprehension of the complex effects of rikkunshito
Zdenka Hromadkova - One of the best experts on this subject based on the ideXlab platform.
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ultrasound assisted extraction of water soluble polysaccharides from the roots of valerian valeriana officinalis l
Ultrasonics Sonochemistry, 2002Co-Authors: Zdenka Hromadkova, Anna Ebringerova, P ValachovicAbstract:Abstract The insoluble plant residues, obtained after preparation of medicinal Tinctures from the roots of valerian (Valeriana officinalis L.) by classical and ultrasound-assisted extraction with aqueous ethanol in a pilot plant, were subsequently treated with hot water to isolate the accessible polysaccharide cell wall components. At almost equal amounts of the hot-water extractable material, the yields of the recovered polysaccharides were lower in the ultrasonical experiment. This is due to the fact that a part of accessible polysaccharides were already solubilised by the aqueous ethanol and recoverable from the medicinal Tincture. Therefore, the net yield of extracted polysaccharides was enhanced in the ultrasonical procedure. This fact as well as the sugar composition and structural features of the isolated polysaccharides suggest that ultrasonication have attacked the integrity of cell walls, released and degraded its most accessible polysaccharides (pectic polysaccharides and starch) and increased also the extractibility of its less accessible components – xylan, mannan and glucan. The water-soluble polysaccharide fractions from both the conventional and ultrasonical experiments exhibit significant immunostimulatory activities in mitogenic and comitogenic thymocyte tests.
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comparison of classical and ultrasound assisted extraction of polysaccharides from salvia officinalis l
Ultrasonics Sonochemistry, 1999Co-Authors: Zdenka Hromadkova, Anna Ebringerova, P ValachovicAbstract:After preparation of medicine Tinctures from the herbal plant Salvia officinalis by classical and ultrasound-assisted extraction with aqueous ethanol, the insoluble plant residues were subsequently treated with hot water and dilute alkali to isolate polysaccharide cell wall components. The yields of the hot water extract as well as total extracted polysaccharides were higher in the case of the ultrasound-treated plant in both laboratory and pilot plant experiments. The water-extractable polysaccharide fractions, in all cases, contained glucose, galactose and arabinose as main sugar components, whereas the alkali-extractable fractions were rich in xylans. The fractions also contained considerable amounts of proteins. The water-soluble polysaccharides may contribute to the biological activity of the plant decoction. The results indicate that the addition of a subsequent extraction step during the preparation of the herbal Tincture might contribute to a better exploitation of the raw material.
Anna Rita Bilia - One of the best experts on this subject based on the ideXlab platform.
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Analysis and stability of the constituents of Curcuma longa and Harpagophytum procumbens Tinctures by HPLC-DAD and HPLC-ESI-MS.
Journal of Pharmaceutical and Biomedical Analysis, 2011Co-Authors: Anastasia Karioti, Elisa Fani, Franco Francesco Vincieri, Anna Rita BiliaAbstract:In the present study two methods based on liquid chromatography with diode array detection (HPLC-DAD) coupled to an electrospray ionisation (ESI-MS) interface were developed for the determination of constituents in the Tinctures (60%, v/v, DER 1:5) of turmeric (Curcuma longa L.) and Devil's claw (Harpagophytum procumbens L.). The developed simple and effective assays permitted the quality control of both Tinctures. The aim of this work was to assess the qualitative and quantitative profile of the constituents of two widely marketed commercial preparations and to evaluate chemical stability of their marker constituents during accelerated thermal stability test by HPLC analysis. Characteristic constituents of C. longa rhizomes are the curcuminoids, whereas characteristic constituents of H. procumbens are acylated iridoid glycosides and phenylethylalcohols. Constituents of Devil's claw Tincture (mainly iridoids) were more stable than curcuminoids of turmeric.
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evaluation of the content and stability of the constituents of mother Tinctures and Tinctures the case of crataegus oxyacantha l and hieracium pilosella l
Journal of Pharmaceutical and Biomedical Analysis, 2007Co-Authors: Anna Rita Bilia, Federico Eterno, Maria Camilla Bergonzi, G Mazzi, Franco Francesco VincieriAbstract:Abstract As a part of our investigations on the content and stability of herbal drug preparations, we evaluated the content and stability of Tinctures and mother Tinctures of Hawthorn leaves and flowers and Hawkweed. Hawthorn preparations are mainly used by patients with cardiac diseases; Hawkweed is employed for the treatment of cellulitis and obesity due to its diuretic properties. Both Tinctures (DER 1:5) and mother Tinctures (DER 1:10) are herbal preparations reported in the European Pharmacopoeia. The first preparation is obtained using dried herbal drugs; the latter is a homoeopathic preparation obtained with fresh plant material, often used in substitution of Tinctures. The aim of this work was to assess the qualitative and quantitative profile of the constituents of the investigated preparations and the chemical stability of their marker constituents from long-term testing using HPLC assays. Characteristic constituents of Hawthorn leaves and flowers are flavonoids such as vitexin-2″- O -rhamnoside and hyperoside and oligomeric procyanidins. Charcteristic constituents of Hawkweed are caffeoyl-quinic acid derivatives, flavonoids and a coumarin:umbelliferone. Our investigation showed that Hawthorn mother Tincture had a higher concentration of procyanidins with respect to the Tincture but the stability of these constituents were very low in both preparations. Total flavonoidic content was 3.33 mg/ml, about 1.5 times more than the content of mother Tincture and the shelf-life t 90 was about 7 months for both preparations. For Hawkweed preparations a content of caffeoyl-quinic acid derivatives (ca. 4 mg/ml) was found, but their stability was good only in the Tincture. The concentrations of flavonoids and umbelliferone were two times as much in the Tincture with respect to the mother Tincture. Stability of these two classes of constituents was good for both preparations over a 9-month period.
