The Experts below are selected from a list of 8442 Experts worldwide ranked by ideXlab platform

Iftikhar Hussain Gul - One of the best experts on this subject based on the ideXlab platform.

Denise J Wedel - One of the best experts on this subject based on the ideXlab platform.

  • Density Specific Gravity and baricity of spinal anesthetic solutions at body temperature
    Anesthesia & Analgesia, 1993
    Co-Authors: Terese T Horlocker, Denise J Wedel
    Abstract:

    One of the most important physical properties affecting the level of analgesia achieved after intrathecal administration of a local anesthetic is its Density relative to the Density of the cerebrospinal fluid (CSF) at 37 degrees C. In this study, Density, Specific Gravity, and baricity of local anesthetic solutions at body temperature were determined volumetrically to five significant figures. Standard solutions of 2% lidocaine, 0.5% and 0.75% bupivacaine, and 0.9% sodium chloride were tested. Bupivacaine, 0.75%-water dilutions and tetracaine, 0.2%, in water were also studied. The densities of all commercially prepared solutions were less than that of the normal range of CSF at 37 degrees C. The Density of 0.2% tetracaine was the same as water. Continued dilution of 0.75% bupivacaine with water resulted in increasingly hypobaric solutions. However, only the 0.075% bupivacaine (1:9 dilution) solution had a Density comparable to water. Knowledge of relative baricities aids in the selection of an appropriate local anesthetic for intrathecal use.

Wei Seng Ho Wei - One of the best experts on this subject based on the ideXlab platform.

  • Gene-associated single nucleotide polymorphism (SNP) in cinnamate 4-hydroxylase (C4H) and cinnamyl alcohol dehydrogenase (CAD) genes from acacia mangium superbulk trees*
    Biotechnology(Faisalabad), 2011
    Co-Authors: Shek Ling Pang Shek, Jusoh Ismail, Ling Tchin Boon, Wei Seng Ho Wei
    Abstract:

    Candidate-gene-based association study which involves the identification of causative Single Nucleotide Polymorphisms (SNPs) for excellent traits has been proposed as a promising approach to dissect complex traits in forest trees. Hence, the goal of this study was to identify the genetic association among SNPs from Cinnamate 4-Hydroxylase (C4H) and Cinnamyl Alcohol Dehydrogenase (CAD) genes and an array of wood properties namely, Specific Gravity, wood Density, fiber-length, cell wall thickness and microfibril angle from Acacia mangium Superbulk trees. Sequence variations within these two genes in 12 A. mangium Superbulk trees were examined and wood properties were measured. The data obtained was tested using General Linear Model (GLM) within TASSEL software. Two SNPs were identified in the exon of C4H, of which all the SNPs caused nonsynonymous mutations whereas five SNPs were identified in the CAD exons along with one deletion mutation. In addition, two SNPs were also identified in the CAD introns. Variation in these two lignin biosynthesis genes might change the structural, functional or biochemical properties of the enzyme being produced, and therefore possibly lead to changes in phenotypic characteristic of the trees. The genetic association study also revealed that SNPs in CAD gene do associate with the wood Density, Specific Gravity and cell wall thickness (p

  • gene associated single nucleotide polymorphism snp in cinnamate 4 hydroxylase c4h and cinnamyl alcohol dehydrogenase cad genes from acacia mangium superbulk trees
    Biotechnology(faisalabad), 2011
    Co-Authors: Shek Ling Pang Shek, Jusoh Ismail, Ling Tchin Boon, Wei Seng Ho Wei
    Abstract:

    Candidate-gene-based association study which involves the identification of causative Single Nucleotide Polymorphisms (SNPs) for excellent traits has been proposed as a promising approach to dissect complex traits in forest trees. Hence, the goal of this study was to identify the genetic association among SNPs from Cinnamate 4-Hydroxylase (C4H) and Cinnamyl Alcohol Dehydrogenase (CAD) genes and an array of wood properties namely, Specific Gravity, wood Density, fiber-length, cell wall thickness and microfibril angle from Acacia mangium Superbulk trees. Sequence variations within these two genes in 12 A. mangium Superbulk trees were examined and wood properties were measured. The data obtained was tested using General Linear Model (GLM) within TASSEL software. Two SNPs were identified in the exon of C4H, of which all the SNPs caused nonsynonymous mutations whereas five SNPs were identified in the CAD exons along with one deletion mutation. In addition, two SNPs were also identified in the CAD introns. Variation in these two lignin biosynthesis genes might change the structural, functional or biochemical properties of the enzyme being produced, and therefore possibly lead to changes in phenotypic characteristic of the trees. The genetic association study also revealed that SNPs in CAD gene do associate with the wood Density, Specific Gravity and cell wall thickness (p<0.05). However, no significant results were obtained for SNPs in C4H gene with wood properties studied. Thickening of cell wall is affected by the arrangement of biopolymer aggregates which comprise of cellulose, hemicellulose and lignin. Results indicated that SNP in CAD gene might alter the lignin biosynthesis and thus lead to changes in phenotypic characteristics of the trees. Overall, the study has demonstrated that SNP is very useful in association genetic study to identify Quantitative Trait Nucleotide (QTN) which then leads to Gene-assisted Selection (GAS) in the tree breeding programme.

Asghari Maqsood - One of the best experts on this subject based on the ideXlab platform.

Terese T Horlocker - One of the best experts on this subject based on the ideXlab platform.

  • Density Specific Gravity and baricity of spinal anesthetic solutions at body temperature
    Anesthesia & Analgesia, 1993
    Co-Authors: Terese T Horlocker, Denise J Wedel
    Abstract:

    One of the most important physical properties affecting the level of analgesia achieved after intrathecal administration of a local anesthetic is its Density relative to the Density of the cerebrospinal fluid (CSF) at 37 degrees C. In this study, Density, Specific Gravity, and baricity of local anesthetic solutions at body temperature were determined volumetrically to five significant figures. Standard solutions of 2% lidocaine, 0.5% and 0.75% bupivacaine, and 0.9% sodium chloride were tested. Bupivacaine, 0.75%-water dilutions and tetracaine, 0.2%, in water were also studied. The densities of all commercially prepared solutions were less than that of the normal range of CSF at 37 degrees C. The Density of 0.2% tetracaine was the same as water. Continued dilution of 0.75% bupivacaine with water resulted in increasingly hypobaric solutions. However, only the 0.075% bupivacaine (1:9 dilution) solution had a Density comparable to water. Knowledge of relative baricities aids in the selection of an appropriate local anesthetic for intrathecal use.