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Marie Abboud - One of the best experts on this subject based on the ideXlab platform.
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Dentinal Tubule penetration of ah plus bc sealer and a novel tricalcium silicate sealer a confocal laser scanning microscopy study
Clinical Oral Investigations, 2019Co-Authors: Roula El Hachem, Issam Khalil, Guy Le Brun, Fabrice Pellen, Bernard Le Jeune, Maha H Daou, Nada El Osta, Alfred Naaman, Marie AbboudAbstract:The aim of this in vitro study was to assess the Dentinal Tubule penetration of three different sealers, AH Plus, BC Sealer and a novel tricalcium silicate sealer (NTS). Ninety-six human maxillary central incisors were divided into three experimental groups (n = 32) and were filled with gutta-percha using a single-cone technique in conjunction with one of the three sealers: AH Plus, BC Sealer or NTS. The roots in each group were cross-sectioned at 1 and 5 mm from the root apex, and the surfaces were examined under confocal laser scanning microscopy (CLSM). The sealer penetration depths were measured at their maximum depths and at four circumferential depths (12, 3, 6 and 9 o’clock) and were evaluated using ImageJ software (ImageJ, NIH). The maximum and mean penetration depths were significantly higher at 5 mm compared to 1 mm from the apex in the AH Plus (p < 0.001), BC Sealer (p < 0.001) and NTS groups (p < 0.001). No significant difference was observed between the groups at 1 mm for both parameters. The maximum and mean penetration depths were significantly lower at 5 mm for AH Plus compared with the other two groups (p = 0.012). Within the study limitations, the BC Sealer and NTS demonstrated better Tubule penetration results than the AH Plus sealer. Although no study has confirmed a relationship between the penetration depth of root canal sealers and the prevention of apical periodontitis, Dentinal Tubule sealer penetration may improve obturation quality.
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influence of the endoactivator irrigation system on Dentinal Tubule penetration of a novel tricalcium silicate based sealer
Dentistry journal, 2018Co-Authors: Roula El Hachem, Issam Khalil, Guy Le Brun, Fabrice Pellen, Bernard Le Jeune, Marie AbboudAbstract:This study compared the effects of a conventional endodontic needle with an agitation system on a novel tricalcium silicate-based sealer (NTS) in terms of Dentinal Tubule penetration and interfacial adaptation to a root canal. Fifty single-rooted, recently-extracted human maxillary central incisors were randomly distributed into two homogeneous groups characterized by two different final cleansing systems: Conventional endodontic needle, or EndoActivator®. After instrumentation, all the teeth were filled with the gutta-percha single cone technique in conjunction with the novel tricalcium silicate-based sealer. Teeth were horizontally sectioned at 1 and 5 mm from the apex and were observed under a confocal laser scanning microscope (CLSM) at five magnifications. The maximum, mean, and the circumferential percentage of the sealer penetration inside the Tubules were measured. Moreover, the gap width was evaluated using Image J software (National Institutes of Health, Bethesda, MD). EndoActivator® did not result in a significantly higher circumferential percentage of sealer penetration than conventional irrigation (p > 0.05). However, the gap width was significantly lower with EndoActivator®, compared to conventional needles at both 1 mm (p = 0.035) and 5 mm (p = 0.038). The EndoActivator® irrigation system did not significantly improve the NTS penetration, as compared to the conventional endodontic needle irrigation. Activation of the irrigation reduced the gap width significantly.
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Influence of the EndoActivator Irrigation System on Dentinal Tubule Penetration of a Novel Tricalcium Silicate-Based Sealer
MDPI AG, 2018Co-Authors: Roula El Hachem, Issam Khalil, Guy Le Brun, Fabrice Pellen, Bernard Le Jeune, Marie AbboudAbstract:This study compared the effects of a conventional endodontic needle with an agitation system on a novel tricalcium silicate-based sealer (NTS) in terms of Dentinal Tubule penetration and interfacial adaptation to a root canal. Fifty single-rooted, recently-extracted human maxillary central incisors were randomly distributed into two homogeneous groups characterized by two different final cleansing systems: Conventional endodontic needle, or EndoActivator®. After instrumentation, all the teeth were filled with the gutta-percha single cone technique in conjunction with the novel tricalcium silicate-based sealer. Teeth were horizontally sectioned at 1 and 5 mm from the apex and were observed under a confocal laser scanning microscope (CLSM) at five magnifications. The maximum, mean, and the circumferential percentage of the sealer penetration inside the Tubules were measured. Moreover, the gap width was evaluated using Image J software (National Institutes of Health, Bethesda, MD). EndoActivator® did not result in a significantly higher circumferential percentage of sealer penetration than conventional irrigation (p > 0.05). However, the gap width was significantly lower with EndoActivator®, compared to conventional needles at both 1 mm (p = 0.035) and 5 mm (p = 0.038). The EndoActivator® irrigation system did not significantly improve the NTS penetration, as compared to the conventional endodontic needle irrigation. Activation of the irrigation reduced the gap width significantly
Nuhan Purali - One of the best experts on this subject based on the ideXlab platform.
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Dentinal Tubule penetration and retreatability of a calcium silicate based sealer tested in bulk or with different main core material
Journal of Endodontics, 2019Co-Authors: Ayhan Eymirli, Derya Deniz Sungur, Nuhan Purali, Ozgur Uyanik, Emre Nagas, Zafer C CehreliAbstract:Abstract Introduction The aim of this study was 2-fold: to evaluate the penetration of a tricalcium silicate–based endodontic sealer (EndoSequence BC Sealer; Brasseler USA, Savannah, GA) into Dentinal Tubules without a core material (sealer) or with .02 or .04 tapered bioceramic gutta-percha points and to compare the time required to remove the root fillings . Methods Roots of extracted human mandibular incisors (N = 60) were prepared with 0.04 taper nickel-titanium rotary files to #35 and randomly assigned into 3 groups (n = 10/group) according to the obturation method used: 1. obturating with sealer only, 2. sealer + .02 point, and 3. sealer + .04 point. The sealer was labeled with rhodamine B for analyzing Dentinal Tubule penetration under a confocal laser scanning microscope. The remaining specimens (n = 30) were used to measure the time for removal of the root canal fillings with retreatment files. The data were analyzed using 1-way analysis of variance and post hoc Games-Howell tests for Dentinal Tubule penetration and the Kruskal-Wallis test for retreatment time. Results Significantly greater sealer penetration and sealer-penetrated area was achieved when the sealer was used with a .04 gutta point (P .05). All test groups showed a similar depth of sealer penetration (P > .05). Groups with the gutta-percha points required a similar time to remove root filling (P > .05), whereas the working length could not be achieved in the sealer group. Conclusions The use of a matched-taper bioceramic gutta-percha point enhanced the Dentinal Tubule penetration of the tested tricalcium silicate–based sealer. The use of a core material in conjunction with sealer facilitates removal of the root filling to the working length.
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effect of ultrasonic activation on Dentinal Tubule penetration of calcium silicate based cements
Microscopy Research and Technique, 2019Co-Authors: Hacer Aksel, Nuhan Purali, Ozgur Uyanik, Elifcan Arslan, Emre NagasAbstract:This study investigated the Dentinal Tubule penetration of mineral trioxide aggregate (MTA), NeoMTA Plus and Biodentine placed by either manual condensation or ultrasonic activation in simulated open apex model. Standardized divergent open apex models were created using palatal roots of 60 human maxillary molars and divided into six groups according to the used cements and activation methods (n = 10): MTA-manual condensation, MTA-ultrasonic activation, NeoMTA Plus-manual condensation, NeoMTA Plus-ultrasonic activation, Biodentine-manual condensation, Biodentine-ultrasonic activation. For the measurement of penetration, the cements were mixed with 0.1% Rhodamin B and 6-mm apical portions of each root canal were obturated in an orthograde direction. The roots were embedded into acrylic blocks, and 1-mm-thick sections were obtained at 3 mm from the apex. Specimens were mounted onto glass slides and scanned under a confocal laser scanning microscope (CLSM) and stereomicroscope. Dentinal Tubule penetration areas, depth and percentage were measured using LSM and ImageJ software. The data were analyzed using two-way analysis of variance (anova) with Bonferroni correction (α = 0.05). No correlation was found between stereomicroscope and CLSM analyses (p > .05). CLSM analysis showed no significant differences between MTA, NeoMTA Plus, and Biodentine groups when manual condensation was used (p > .05). Ultrasonic activation did not increase the tubular penetration of MTA, NeoMTA Plus or Biodentine as compared to manual condensation of each material (p > .05). MTA, NeoMTA Plus and Biodentine showed similar tubular penetration when manual condensation was used. Ultrasonic activation of these cements had no effect on tubular penetration of each material as compared to the manual condensation counterparts.
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evaluation of Dentinal Tubule penetration depth and push out bond strength of ah 26 bioroot rcs and mta plus root canal sealers in presence or absence of smear layer
Journal of dental research dental clinics dental prospects, 2018Co-Authors: Sevinc Aktemur Turker, Emel Uzunoglu, Nuhan PuraliAbstract:Background. This study compared the effect of smear layer on the penetration depth and push-out bond strength of various root canal sealers. Methods. A total of 90 extracted human mandibular premolars were assigned into 2 groups: smear layer preserved and smear layer removed. Then the roots were further divided into 3 subgroups according to the sealer tested: AH 26, BioRoot RCS and MTA Plus. Obturation was performed with gutta-percha and the relevant sealer was mixed with 0.1% rhodamine B. Three 1-mm-thick slices were obtained from the mid-third area of each root. Two slices were selected for the push-out test and the remaining slice was used to calculate the Dentinal Tubule penetration depth and percentage. Results. The retention of MTA Plus and BioRoot RCS was higher than that of AH 26 when the smear layer was preserved (P<0.05). BioRoot RCS showed the lowest penetration depth when the smear layer was removed (P<0.05). Conclusion. Dentinal Tubule penetration of root canal sealers had a limited effect on their adhesion to root canal wall.
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Evaluation of Dentinal Tubule penetration depth and push-out bond strength of AH 26, BioRoot RCS, and MTA Plus root canal sealers in presence or absence of smear layer
Tabriz University of Medical Sciences, 2018Co-Authors: Sevinc Aktemur Turker, Emel Uzunoglu, Nuhan PuraliAbstract:Background.This study compared the effect of smear layer on the penetration depth and push-out bond strength of various root canal sealers. Methods.A total of 90 extracted human mandibular premolars were assigned into 2 groups: smear layer preserved and smear layer removed. Then the roots were further divided into 3 subgroups according to the sealer tested: AH 26, BioRoot RCS and MTA Plus. Obturation was performed with gutta-percha and the relevant sealer was mixed with 0.1% rhodamine B. Three 1-mm-thick slices were obtained from the mid-third area of each root. Two slices were selected for the push-out test and the remaining slice was used to calculate the Dentinal Tubule penetration depth and percentage. Results.The retention of MTA Plus and BioRoot RCS was higher than that of AH 26 when the smear layer was preserved (P
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Effect of a Low Surface Tension Vehicle on the Dentinal Tubule Penetration of Calcium Hydroxide and Triple Antibiotic Paste.
Journal of endodontics, 2017Co-Authors: Derya Deniz Sungur, Hacer Aksel, Nuhan PuraliAbstract:Abstract Introduction The purpose of this study was to evaluate Dentinal Tubule penetration (DTP) of calcium hydroxide (CH) and triple antibiotic paste (TAP) when performed with distilled water (DW) or a low surface tension liquid (ie, propylene glycol [PG]). Methods Root apices of 40 single-rooted premolars were removed to obtain 14-mm roots in length. Root canals were enlarged to simulate immature teeth. After smear layer removal, the roots were randomly divided into 4 groups ( n = 10) according to the root canal medicaments and the vehicles used: group 1:TAP + DW, group 2: TAP + PG, group 3: CH + DW, and group 4:CH + PG. Root canal medicaments were labeled with 0.1% rhodamine and applied into the canals using a Lentulo spiral. Specimens were molded into acrylic blocks, and 1-mm-thick sections were obtained from the middle third of each root. Specimens were mounted onto glass slides and scanned under a confocal laser scanning microscope. DTP depth, percentage, and area were measured using imaging software. Kruskal-Wallis and Mann-Whitney U tests were used for statistical analysis. The level of significance was set at P Results No significant difference was found among the experimental groups in terms of both percentage and depth of DTP ( P > .05). CH had a lower penetration area compared with TAP regardless of the vehicle used ( P Conclusions A low surface tension vehicle did not alter the penetration of CH and TAP.
Roula El Hachem - One of the best experts on this subject based on the ideXlab platform.
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Dentinal Tubule penetration of ah plus bc sealer and a novel tricalcium silicate sealer a confocal laser scanning microscopy study
Clinical Oral Investigations, 2019Co-Authors: Roula El Hachem, Issam Khalil, Guy Le Brun, Fabrice Pellen, Bernard Le Jeune, Maha H Daou, Nada El Osta, Alfred Naaman, Marie AbboudAbstract:The aim of this in vitro study was to assess the Dentinal Tubule penetration of three different sealers, AH Plus, BC Sealer and a novel tricalcium silicate sealer (NTS). Ninety-six human maxillary central incisors were divided into three experimental groups (n = 32) and were filled with gutta-percha using a single-cone technique in conjunction with one of the three sealers: AH Plus, BC Sealer or NTS. The roots in each group were cross-sectioned at 1 and 5 mm from the root apex, and the surfaces were examined under confocal laser scanning microscopy (CLSM). The sealer penetration depths were measured at their maximum depths and at four circumferential depths (12, 3, 6 and 9 o’clock) and were evaluated using ImageJ software (ImageJ, NIH). The maximum and mean penetration depths were significantly higher at 5 mm compared to 1 mm from the apex in the AH Plus (p < 0.001), BC Sealer (p < 0.001) and NTS groups (p < 0.001). No significant difference was observed between the groups at 1 mm for both parameters. The maximum and mean penetration depths were significantly lower at 5 mm for AH Plus compared with the other two groups (p = 0.012). Within the study limitations, the BC Sealer and NTS demonstrated better Tubule penetration results than the AH Plus sealer. Although no study has confirmed a relationship between the penetration depth of root canal sealers and the prevention of apical periodontitis, Dentinal Tubule sealer penetration may improve obturation quality.
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influence of the endoactivator irrigation system on Dentinal Tubule penetration of a novel tricalcium silicate based sealer
Dentistry journal, 2018Co-Authors: Roula El Hachem, Issam Khalil, Guy Le Brun, Fabrice Pellen, Bernard Le Jeune, Marie AbboudAbstract:This study compared the effects of a conventional endodontic needle with an agitation system on a novel tricalcium silicate-based sealer (NTS) in terms of Dentinal Tubule penetration and interfacial adaptation to a root canal. Fifty single-rooted, recently-extracted human maxillary central incisors were randomly distributed into two homogeneous groups characterized by two different final cleansing systems: Conventional endodontic needle, or EndoActivator®. After instrumentation, all the teeth were filled with the gutta-percha single cone technique in conjunction with the novel tricalcium silicate-based sealer. Teeth were horizontally sectioned at 1 and 5 mm from the apex and were observed under a confocal laser scanning microscope (CLSM) at five magnifications. The maximum, mean, and the circumferential percentage of the sealer penetration inside the Tubules were measured. Moreover, the gap width was evaluated using Image J software (National Institutes of Health, Bethesda, MD). EndoActivator® did not result in a significantly higher circumferential percentage of sealer penetration than conventional irrigation (p > 0.05). However, the gap width was significantly lower with EndoActivator®, compared to conventional needles at both 1 mm (p = 0.035) and 5 mm (p = 0.038). The EndoActivator® irrigation system did not significantly improve the NTS penetration, as compared to the conventional endodontic needle irrigation. Activation of the irrigation reduced the gap width significantly.
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Influence of the EndoActivator Irrigation System on Dentinal Tubule Penetration of a Novel Tricalcium Silicate-Based Sealer
MDPI AG, 2018Co-Authors: Roula El Hachem, Issam Khalil, Guy Le Brun, Fabrice Pellen, Bernard Le Jeune, Marie AbboudAbstract:This study compared the effects of a conventional endodontic needle with an agitation system on a novel tricalcium silicate-based sealer (NTS) in terms of Dentinal Tubule penetration and interfacial adaptation to a root canal. Fifty single-rooted, recently-extracted human maxillary central incisors were randomly distributed into two homogeneous groups characterized by two different final cleansing systems: Conventional endodontic needle, or EndoActivator®. After instrumentation, all the teeth were filled with the gutta-percha single cone technique in conjunction with the novel tricalcium silicate-based sealer. Teeth were horizontally sectioned at 1 and 5 mm from the apex and were observed under a confocal laser scanning microscope (CLSM) at five magnifications. The maximum, mean, and the circumferential percentage of the sealer penetration inside the Tubules were measured. Moreover, the gap width was evaluated using Image J software (National Institutes of Health, Bethesda, MD). EndoActivator® did not result in a significantly higher circumferential percentage of sealer penetration than conventional irrigation (p > 0.05). However, the gap width was significantly lower with EndoActivator®, compared to conventional needles at both 1 mm (p = 0.035) and 5 mm (p = 0.038). The EndoActivator® irrigation system did not significantly improve the NTS penetration, as compared to the conventional endodontic needle irrigation. Activation of the irrigation reduced the gap width significantly
Chun-pin Lin - One of the best experts on this subject based on the ideXlab platform.
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The dentin permeability of anti-inflammatory and antibacterial drugs: In vitro study
Elsevier, 2019Co-Authors: Chun-pei Lin, Yin-lin Wang, Li-juan Shen, Chun-pin LinAbstract:Background/purpose: Stimuli from the oral cavity may penetrate through exposed Dentinal Tubules and evoke inflammatory pulp response. Anti-bacterial and anti-inflammatory drugs applied to exposed dentin may infiltrate through the Dentinal Tubules and cause pulp recovery. This study investigated the dentin permeability of anti-bacterial and anti-inflammation drugs via an in-vitro transwell dentin disc tube model. Methods: Twenty-seven dentin discs prepared from extracted human molars were collected. Nine kinds of drugs were investigated with three dentin discs in each group. These nine drugs included two anti-bacterial drugs (ampicillin sodium and clindamycin phosphate), two corticosteroids (betamethasone sodium phosphate and hydrocortisone sodium succinate), three non-steroidal anti-inflammatory drugs (NSAIDs, piroxicam, lysine acetylsalicylate, and diclofenac sodium), and two natural extracts with anti-inflammatory effect (Ginsenoside Rg1 and Hinokitol). The drugs were introduced to the transwell dentin disc tube model and the 4-hour cumulative release of the drug was detected and recorded by UV-visible spectroscopy. Results: We found that ampicilin sodium had better dentin permeability than clindamycin phosphate. Betamethasone sodium phosphate revealed better dentin permeability than hydrocortisone sodium succinate. Lysine acetylsalicylate showed the best dentin permeability among the three NSAIDs. Ginsenoside Rg1 had the best dentin permeability among the nine drugs tested. However, Hinokitiol could not penetrate the dentin disc after 4 h. Conclusion: Regarding the dentin permeability, Ginsenoside Rg1 is the best among the seven anti-inflammatory drugs tested and ampicilin sodium is the better one between the two anti-bacterial drugs tested. Therefore, these two drugs may have high potential for treating exposed Dentinal Tubule diseases. Keywords: Anti-bacterial agents, Anti-inflammatory agents, Dentin permeability, Exposed Dentinal Tubule
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The combined occluding effect of sodium fluoride varnish and Nd:YAG laser irradiation on human Dentinal Tubules.
Journal of endodontics, 1999Co-Authors: Wan-hong Lan, Hsin-cheng Liu, Chun-pin LinAbstract:Various methods and materials used in the treatment of dentin hypersensitivity are thought to achieve a therapeutic benefit by Tubule occlusion. The aim of the present study was to evaluate the combined occluding effect of sodium fluoride varnish and Nd:YAG laser irradiation on human Dentinal Tubules. Thirty-six dentin specimens with exposed Dentinal Tubule orifices were used in this study. The samples were randomly divided into four groups. Groups A, B, and C were varnished by sodium fluoride, whereas group D served as a control. Then, group C was lased by 30 mJ of Nd:YAG laser, 10 pulses/s for 2 min by light painting. Three hours later, groups B and C were brushed by an electrical toothbrush for 30 min. Under SEM observation, the control group showed numerous exposed Dentinal Tubule orifices, and the sodium fluoride varnished specimens showed closure of exposed Dentinal Tubule orifices. After electrical toothbrushing, most of the sodium fluoride varnish was brushed away, except in the specimens that were irradiated by Nd:YAG laser. Over 90% of the Dentinal Tubule orifices were occluded by sodium fluoride varnish combined with Nd:YAG laser irradiation.
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Sealing depth of Nd:YAG laser on human Dentinal Tubules.
Journal of endodontics, 1997Co-Authors: Hsin-cheng Liu, Chun-pin Lin, Wan-hong LanAbstract:Dentin permeability and hypersensitivity are both reduced when the Dentinal Tubules are occluded. Previous scanning electron microscopic studies showed that Nd:YAG laser could cause melting of dentin and closure of exposed Dentinal Tubules without dentin surface cracking. Therefore, the aim of the present study was to evaluate the sealing depth of Nd:YAG laser on human Dentinal Tubules. Thirty-six dentin specimens with exposed Dentinal Tubule orifices were used in this study. Samples were randomly divided into three groups. Groups A and B were lased by Nd:YAG laser at energy of 30 mJ with 10 pulses/s for a stroke along the dentin surface. Group C was not lased and served as a control. Subsequently, group B was frozen in liquid nitrogen and split by a sharp chisel. Under SEM observation, nonlased specimens showed numerous exposed Dentinal Tubule orifices, and lased specimens showed melting of dentin and closure of exposed Dentinal Tubule orifices. The sealing depth of Nd:YAG laser on human Dentinal Tubules was ∼4 μm.
Wan-hong Lan - One of the best experts on this subject based on the ideXlab platform.
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The combined occluding effect of sodium fluoride varnish and Nd:YAG laser irradiation on human Dentinal Tubules.
Journal of endodontics, 1999Co-Authors: Wan-hong Lan, Hsin-cheng Liu, Chun-pin LinAbstract:Various methods and materials used in the treatment of dentin hypersensitivity are thought to achieve a therapeutic benefit by Tubule occlusion. The aim of the present study was to evaluate the combined occluding effect of sodium fluoride varnish and Nd:YAG laser irradiation on human Dentinal Tubules. Thirty-six dentin specimens with exposed Dentinal Tubule orifices were used in this study. The samples were randomly divided into four groups. Groups A, B, and C were varnished by sodium fluoride, whereas group D served as a control. Then, group C was lased by 30 mJ of Nd:YAG laser, 10 pulses/s for 2 min by light painting. Three hours later, groups B and C were brushed by an electrical toothbrush for 30 min. Under SEM observation, the control group showed numerous exposed Dentinal Tubule orifices, and the sodium fluoride varnished specimens showed closure of exposed Dentinal Tubule orifices. After electrical toothbrushing, most of the sodium fluoride varnish was brushed away, except in the specimens that were irradiated by Nd:YAG laser. Over 90% of the Dentinal Tubule orifices were occluded by sodium fluoride varnish combined with Nd:YAG laser irradiation.
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Sealing depth of Nd:YAG laser on human Dentinal Tubules.
Journal of endodontics, 1997Co-Authors: Hsin-cheng Liu, Chun-pin Lin, Wan-hong LanAbstract:Dentin permeability and hypersensitivity are both reduced when the Dentinal Tubules are occluded. Previous scanning electron microscopic studies showed that Nd:YAG laser could cause melting of dentin and closure of exposed Dentinal Tubules without dentin surface cracking. Therefore, the aim of the present study was to evaluate the sealing depth of Nd:YAG laser on human Dentinal Tubules. Thirty-six dentin specimens with exposed Dentinal Tubule orifices were used in this study. Samples were randomly divided into three groups. Groups A and B were lased by Nd:YAG laser at energy of 30 mJ with 10 pulses/s for a stroke along the dentin surface. Group C was not lased and served as a control. Subsequently, group B was frozen in liquid nitrogen and split by a sharp chisel. Under SEM observation, nonlased specimens showed numerous exposed Dentinal Tubule orifices, and lased specimens showed melting of dentin and closure of exposed Dentinal Tubule orifices. The sealing depth of Nd:YAG laser on human Dentinal Tubules was ∼4 μm.