The Experts below are selected from a list of 11913 Experts worldwide ranked by ideXlab platform
Peter Mwaura - One of the best experts on this subject based on the ideXlab platform.
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resistance and tolerance of potato varieties to potato rot nematode ditylenchus destructor and stem nematode ditylenchus dipsaci
Annals of Applied Biology, 2015Co-Authors: Peter Mwaura, Bjorn Niere, Stefan VidalAbstract:Ditylenchus destructor and Ditylenchus dipsaci are economically important plant-parasitic nematodes, affecting potato production mostly in temperate climates. Management through crop rotation is not feasible because of their wide host range. These nematodes are listed as quarantine pests in many countries. Limited information exists on the resistance and tolerance of currently cultivated potatoes to D. destructor and D. dipsaci. Two greenhouse experiments were conducted to screen 25 potato varieties for resistance to and tolerance for D. destructor and D. dipsaci infections. Reproduction factor (RF) and relative susceptibility (RS) were used to evaluate resistance, while potato tuber damage and tuber weight reduction was used to evaluate tolerance. Based on the RF, 16 varieties were evaluated as susceptible (S) while 5 varieties were evaluated as resistant (R) to D. destructor; varieties ‘Innovator’, ‘Aveka’ and ‘Spunta’ were resistant to D. dipsaci based on RF. ‘Desiree’ was observed to be highly susceptible to D. destructor and D. dipsaci in both experiments and was used as the standard susceptible control variety for the calculation of RS. A scale of 1–9 was used to classify RS of the potato varieties to D. destructor and D. dipsaci, where 9 indicated the highest level of resistance. All classes of resistance to D. destructor and D. dipsaci were observed in the potato varieties tested in the experiments. Six varieties had significantly lower RS to D. dipsaci than the standard susceptible control variety. Tolerant to highly sensitive potato varieties to both nematodes were also observed. RS and external potato tuber damage were identified as suitable methods for resistance and tolerance determination, respectively. This study provides essential information on the status of resistance and tolerance in potato varieties against D. destructor and D. dipsaci but needs to be confirmed under field conditions.
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effect of initial population densities of ditylenchus destructor and d dipsaci on potato tuber damage and nematode reproduction
Nematology, 2015Co-Authors: Peter Mwaura, Bjorn Niere, Stefan VidalAbstract:Glasshouse experiments were conducted to evaluate the effect of initial population densities (Pi) of Ditylenchus destructor and D. dipsaci on potato tuber damage and nematode reproduction. Ditylenchus destructor did not influence tuber numbers but influenced tuber weight at high Pi levels. Ditylenchus dipsaci influenced tuber numbers and weights at a Pi level of 14.29 (g growing medium)−1. Tolerance limit estimates according to the Seinhorst model were very low indicating both nematode species have a major impact on potato tuber weight. External and internal tuber rot caused by both species increased with Pi levels. Ditylenchus destructor caused more tuber rot than D. dipsaci at all Pi levels. Reproduction rates of D. destructor were higher at all Pi levels studied compared to D. dipsaci. The equilibrium density of 1.3 and 0.6 for D. destructor and D. dipsaci, respectively, was observed at Pi level of 14.29 (g growing medium)−1.
Marianne Van Hagehamsten - One of the best experts on this subject based on the ideXlab platform.
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cloning of three new allergens from the dust mite lepidoglyphus destructor using phage surface display technology
FEBS Journal, 2001Co-Authors: Tove L J Eriksson, Omid Rasool, Sonia Huecas, Ulrich Appenzeller, Guro Gafvelin, Paul Whitley, Reto Crameri, Marianne Van HagehamstenAbstract:The dust mite Lepidoglyphus destructor is a common species in Europe and a major cause of dust mite allergy in rural surroundings, but it also contributes to dust mite allergy in urban areas. One major allergen, Lep d 2, has been expressed as a recombinant protein and evaluated both in vivo and in vitro and shown to detect 60% or more of L. destructor-sensitized subjects. Additional recombinant allergens are needed to obtain a reliable diagnostic tool for L. destructor allergy. The aim of this study was to clone and express new allergens from L. destructor and determine their recognition frequency among sensitized individuals. A phage display cDNA expression library was constructed and screened with sera from L. destructor-sensitized individuals. The cDNAs encoding the allergens were cloned into the pET17b vector and subsequently expressed in Escherichia coli as C-terminal His6-tagged proteins. Immunoblotting of the recombinant proteins was performed using sera from 45 subjects allergic to L. destructor. Three new allergens from L. destructor, Ld 5 (originating from a partial Lep d 5 clone), Lep d 7 and Lep d 13, were identified and recognized by 4/45 (9%), 28/45 (62%) and 6/45 (13%) sera from L. destructor-sensitized subjects, respectively.
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cloning of three new allergens from the dust mite lepidoglyphus destructor using phage surface display technology
FEBS Journal, 2001Co-Authors: Tove L J Eriksson, Omid Rasool, Sonia Huecas, Ulrich Appenzeller, Guro Gafvelin, Paul Whitley, Reto Crameri, Marianne Van HagehamstenAbstract:The dust mite Lepidoglyphus destructor is a common species in Europe and a major cause of dust mite allergy in rural surroundings, but it also contributes to dust mite allergy in urban areas. One major allergen, Lep d 2, has been expressed as a recombinant protein and evaluated both in vivo and in vitro and shown to detect 60% or more of L. destructor-sensitized subjects. Additional recombinant allergens are needed to obtain a reliable diagnostic tool for L. destructor allergy. The aim of this study was to clone and express new allergens from L. destructor and determine their recognition frequency among sensitized individuals. A phage display cDNA expression library was constructed and screened with sera from L. destructor-sensitized individuals. The cDNAs encoding the allergens were cloned into the pET17b vector and subsequently expressed in Escherichia coli as C-terminal His6-tagged proteins. Immunoblotting of the recombinant proteins was performed using sera from 45 subjects allergic to L. destructor. Three new allergens from L. destructor, Ld 5 (originating from a partial Lep d 5 clone), Lep d 7 and Lep d 13, were identified and recognized by 4/45 (9%), 28/45 (62%) and 6/45 (13%) sera from L. destructor-sensitized subjects, respectively.
Stefan Vidal - One of the best experts on this subject based on the ideXlab platform.
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resistance and tolerance of potato varieties to potato rot nematode ditylenchus destructor and stem nematode ditylenchus dipsaci
Annals of Applied Biology, 2015Co-Authors: Peter Mwaura, Bjorn Niere, Stefan VidalAbstract:Ditylenchus destructor and Ditylenchus dipsaci are economically important plant-parasitic nematodes, affecting potato production mostly in temperate climates. Management through crop rotation is not feasible because of their wide host range. These nematodes are listed as quarantine pests in many countries. Limited information exists on the resistance and tolerance of currently cultivated potatoes to D. destructor and D. dipsaci. Two greenhouse experiments were conducted to screen 25 potato varieties for resistance to and tolerance for D. destructor and D. dipsaci infections. Reproduction factor (RF) and relative susceptibility (RS) were used to evaluate resistance, while potato tuber damage and tuber weight reduction was used to evaluate tolerance. Based on the RF, 16 varieties were evaluated as susceptible (S) while 5 varieties were evaluated as resistant (R) to D. destructor; varieties ‘Innovator’, ‘Aveka’ and ‘Spunta’ were resistant to D. dipsaci based on RF. ‘Desiree’ was observed to be highly susceptible to D. destructor and D. dipsaci in both experiments and was used as the standard susceptible control variety for the calculation of RS. A scale of 1–9 was used to classify RS of the potato varieties to D. destructor and D. dipsaci, where 9 indicated the highest level of resistance. All classes of resistance to D. destructor and D. dipsaci were observed in the potato varieties tested in the experiments. Six varieties had significantly lower RS to D. dipsaci than the standard susceptible control variety. Tolerant to highly sensitive potato varieties to both nematodes were also observed. RS and external potato tuber damage were identified as suitable methods for resistance and tolerance determination, respectively. This study provides essential information on the status of resistance and tolerance in potato varieties against D. destructor and D. dipsaci but needs to be confirmed under field conditions.
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effect of initial population densities of ditylenchus destructor and d dipsaci on potato tuber damage and nematode reproduction
Nematology, 2015Co-Authors: Peter Mwaura, Bjorn Niere, Stefan VidalAbstract:Glasshouse experiments were conducted to evaluate the effect of initial population densities (Pi) of Ditylenchus destructor and D. dipsaci on potato tuber damage and nematode reproduction. Ditylenchus destructor did not influence tuber numbers but influenced tuber weight at high Pi levels. Ditylenchus dipsaci influenced tuber numbers and weights at a Pi level of 14.29 (g growing medium)−1. Tolerance limit estimates according to the Seinhorst model were very low indicating both nematode species have a major impact on potato tuber weight. External and internal tuber rot caused by both species increased with Pi levels. Ditylenchus destructor caused more tuber rot than D. dipsaci at all Pi levels. Reproduction rates of D. destructor were higher at all Pi levels studied compared to D. dipsaci. The equilibrium density of 1.3 and 0.6 for D. destructor and D. dipsaci, respectively, was observed at Pi level of 14.29 (g growing medium)−1.
Tove L J Eriksson - One of the best experts on this subject based on the ideXlab platform.
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cloning of three new allergens from the dust mite lepidoglyphus destructor using phage surface display technology
FEBS Journal, 2001Co-Authors: Tove L J Eriksson, Omid Rasool, Sonia Huecas, Ulrich Appenzeller, Guro Gafvelin, Paul Whitley, Reto Crameri, Marianne Van HagehamstenAbstract:The dust mite Lepidoglyphus destructor is a common species in Europe and a major cause of dust mite allergy in rural surroundings, but it also contributes to dust mite allergy in urban areas. One major allergen, Lep d 2, has been expressed as a recombinant protein and evaluated both in vivo and in vitro and shown to detect 60% or more of L. destructor-sensitized subjects. Additional recombinant allergens are needed to obtain a reliable diagnostic tool for L. destructor allergy. The aim of this study was to clone and express new allergens from L. destructor and determine their recognition frequency among sensitized individuals. A phage display cDNA expression library was constructed and screened with sera from L. destructor-sensitized individuals. The cDNAs encoding the allergens were cloned into the pET17b vector and subsequently expressed in Escherichia coli as C-terminal His6-tagged proteins. Immunoblotting of the recombinant proteins was performed using sera from 45 subjects allergic to L. destructor. Three new allergens from L. destructor, Ld 5 (originating from a partial Lep d 5 clone), Lep d 7 and Lep d 13, were identified and recognized by 4/45 (9%), 28/45 (62%) and 6/45 (13%) sera from L. destructor-sensitized subjects, respectively.
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cloning of three new allergens from the dust mite lepidoglyphus destructor using phage surface display technology
FEBS Journal, 2001Co-Authors: Tove L J Eriksson, Omid Rasool, Sonia Huecas, Ulrich Appenzeller, Guro Gafvelin, Paul Whitley, Reto Crameri, Marianne Van HagehamstenAbstract:The dust mite Lepidoglyphus destructor is a common species in Europe and a major cause of dust mite allergy in rural surroundings, but it also contributes to dust mite allergy in urban areas. One major allergen, Lep d 2, has been expressed as a recombinant protein and evaluated both in vivo and in vitro and shown to detect 60% or more of L. destructor-sensitized subjects. Additional recombinant allergens are needed to obtain a reliable diagnostic tool for L. destructor allergy. The aim of this study was to clone and express new allergens from L. destructor and determine their recognition frequency among sensitized individuals. A phage display cDNA expression library was constructed and screened with sera from L. destructor-sensitized individuals. The cDNAs encoding the allergens were cloned into the pET17b vector and subsequently expressed in Escherichia coli as C-terminal His6-tagged proteins. Immunoblotting of the recombinant proteins was performed using sera from 45 subjects allergic to L. destructor. Three new allergens from L. destructor, Ld 5 (originating from a partial Lep d 5 clone), Lep d 7 and Lep d 13, were identified and recognized by 4/45 (9%), 28/45 (62%) and 6/45 (13%) sera from L. destructor-sensitized subjects, respectively.
Guro Gafvelin - One of the best experts on this subject based on the ideXlab platform.
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cloning of three new allergens from the dust mite lepidoglyphus destructor using phage surface display technology
FEBS Journal, 2001Co-Authors: Tove L J Eriksson, Omid Rasool, Sonia Huecas, Ulrich Appenzeller, Guro Gafvelin, Paul Whitley, Reto Crameri, Marianne Van HagehamstenAbstract:The dust mite Lepidoglyphus destructor is a common species in Europe and a major cause of dust mite allergy in rural surroundings, but it also contributes to dust mite allergy in urban areas. One major allergen, Lep d 2, has been expressed as a recombinant protein and evaluated both in vivo and in vitro and shown to detect 60% or more of L. destructor-sensitized subjects. Additional recombinant allergens are needed to obtain a reliable diagnostic tool for L. destructor allergy. The aim of this study was to clone and express new allergens from L. destructor and determine their recognition frequency among sensitized individuals. A phage display cDNA expression library was constructed and screened with sera from L. destructor-sensitized individuals. The cDNAs encoding the allergens were cloned into the pET17b vector and subsequently expressed in Escherichia coli as C-terminal His6-tagged proteins. Immunoblotting of the recombinant proteins was performed using sera from 45 subjects allergic to L. destructor. Three new allergens from L. destructor, Ld 5 (originating from a partial Lep d 5 clone), Lep d 7 and Lep d 13, were identified and recognized by 4/45 (9%), 28/45 (62%) and 6/45 (13%) sera from L. destructor-sensitized subjects, respectively.
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cloning of three new allergens from the dust mite lepidoglyphus destructor using phage surface display technology
FEBS Journal, 2001Co-Authors: Tove L J Eriksson, Omid Rasool, Sonia Huecas, Ulrich Appenzeller, Guro Gafvelin, Paul Whitley, Reto Crameri, Marianne Van HagehamstenAbstract:The dust mite Lepidoglyphus destructor is a common species in Europe and a major cause of dust mite allergy in rural surroundings, but it also contributes to dust mite allergy in urban areas. One major allergen, Lep d 2, has been expressed as a recombinant protein and evaluated both in vivo and in vitro and shown to detect 60% or more of L. destructor-sensitized subjects. Additional recombinant allergens are needed to obtain a reliable diagnostic tool for L. destructor allergy. The aim of this study was to clone and express new allergens from L. destructor and determine their recognition frequency among sensitized individuals. A phage display cDNA expression library was constructed and screened with sera from L. destructor-sensitized individuals. The cDNAs encoding the allergens were cloned into the pET17b vector and subsequently expressed in Escherichia coli as C-terminal His6-tagged proteins. Immunoblotting of the recombinant proteins was performed using sera from 45 subjects allergic to L. destructor. Three new allergens from L. destructor, Ld 5 (originating from a partial Lep d 5 clone), Lep d 7 and Lep d 13, were identified and recognized by 4/45 (9%), 28/45 (62%) and 6/45 (13%) sera from L. destructor-sensitized subjects, respectively.