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Bernard Ollivier - One of the best experts on this subject based on the ideXlab platform.
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reclassification of the sulfate and nitrate reducing bacterium Desulfovibrio vulgaris subsp oxamicus as Desulfovibrio oxamicus sp nov comb nov
International Journal of Systematic and Evolutionary Microbiology, 2006Co-Authors: Alejandro Lopezcortes, Guy Fauque, Marie-laure Fardeau, Catherine Joulian, Bernard OllivierAbstract:Desulfovibrio vulgaris subsp. oxamicus (type strain, DSM 1925T) was found to use nitrate as a terminal electron acceptor, the latter being reduced to ammonium. Phylogenetic studies indicated that strain DSM 1925T was distantly related to the type strain of Desulfovibrio vulgaris (95.4 % similarity of the small-subunit rRNA gene) and had as its closest phylogenetic relatives two other nitrate- and sulfate-reducing bacteria, namely Desulfovibrio termitidis (99.4 % similarity) and Desulfovibrio longreachensis (98.4 % similarity). Additional experiments were conducted to characterize better strain DSM 1925T. This strain incompletely oxidized lactate and ethanol to acetate. It also oxidized butanol, pyruvate and citrate, but not glucose, fructose, acetate, propionate, butyrate, methanol, glycerol or peptone. The optimum temperature for growth was 37 °C (range 16–50 °C) and the optimum NaCl concentration for growth was 0.1 % (range 0–5 %). Because of significant genotypic and phenotypic differences from Desulfovibrio termitidis and Desulfovibrio longreachensis, reclassification of Desulfovibrio vulgaris subsp. oxamicus as Desulfovibrio oxamicus sp. nov., comb. nov., is proposed. The type strain is strain Monticello 2T (=DSM 1925T=NCIMB 9442T=ATCC 33405T).
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Reclassification of the sulfate- and nitrate-reducing bacterium Desulfovibrio vulgaris subsp. oxamicus as Desulfovibrio oxamicus sp. nov., comb. nov.
International journal of systematic and evolutionary microbiology, 2006Co-Authors: Alejandro López-cortés, Guy Fauque, Marie-laure Fardeau, Catherine Joulian, Bernard OllivierAbstract:Desulfovibrio vulgaris subsp. oxamicus (type strain, DSM 1925(T)) was found to use nitrate as a terminal electron acceptor, the latter being reduced to ammonium. Phylogenetic studies indicated that strain DSM 1925(T) was distantly related to the type strain of Desulfovibrio vulgaris (95.4 % similarity of the small-subunit rRNA gene) and had as its closest phylogenetic relatives two other nitrate- and sulfate-reducing bacteria, namely Desulfovibrio termitidis (99.4 % similarity) and Desulfovibrio longreachensis (98.4 % similarity). Additional experiments were conducted to characterize better strain DSM 1925(T). This strain incompletely oxidized lactate and ethanol to acetate. It also oxidized butanol, pyruvate and citrate, but not glucose, fructose, acetate, propionate, butyrate, methanol, glycerol or peptone. The optimum temperature for growth was 37 degrees C (range 16-50 degrees C) and the optimum NaCl concentration for growth was 0.1 % (range 0-5 %). Because of significant genotypic and phenotypic differences from Desulfovibrio termitidis and Desulfovibrio longreachensis, reclassification of Desulfovibrio vulgaris subsp. oxamicus as Desulfovibrio oxamicus sp. nov., comb. nov., is proposed. The type strain is strain Monticello 2(T) (=DSM 1925(T)=NCIMB 9442(T)=ATCC 33405(T)).
Guy Fauque - One of the best experts on this subject based on the ideXlab platform.
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reclassification of the sulfate and nitrate reducing bacterium Desulfovibrio vulgaris subsp oxamicus as Desulfovibrio oxamicus sp nov comb nov
International Journal of Systematic and Evolutionary Microbiology, 2006Co-Authors: Alejandro Lopezcortes, Guy Fauque, Marie-laure Fardeau, Catherine Joulian, Bernard OllivierAbstract:Desulfovibrio vulgaris subsp. oxamicus (type strain, DSM 1925T) was found to use nitrate as a terminal electron acceptor, the latter being reduced to ammonium. Phylogenetic studies indicated that strain DSM 1925T was distantly related to the type strain of Desulfovibrio vulgaris (95.4 % similarity of the small-subunit rRNA gene) and had as its closest phylogenetic relatives two other nitrate- and sulfate-reducing bacteria, namely Desulfovibrio termitidis (99.4 % similarity) and Desulfovibrio longreachensis (98.4 % similarity). Additional experiments were conducted to characterize better strain DSM 1925T. This strain incompletely oxidized lactate and ethanol to acetate. It also oxidized butanol, pyruvate and citrate, but not glucose, fructose, acetate, propionate, butyrate, methanol, glycerol or peptone. The optimum temperature for growth was 37 °C (range 16–50 °C) and the optimum NaCl concentration for growth was 0.1 % (range 0–5 %). Because of significant genotypic and phenotypic differences from Desulfovibrio termitidis and Desulfovibrio longreachensis, reclassification of Desulfovibrio vulgaris subsp. oxamicus as Desulfovibrio oxamicus sp. nov., comb. nov., is proposed. The type strain is strain Monticello 2T (=DSM 1925T=NCIMB 9442T=ATCC 33405T).
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Reclassification of the sulfate- and nitrate-reducing bacterium Desulfovibrio vulgaris subsp. oxamicus as Desulfovibrio oxamicus sp. nov., comb. nov.
International journal of systematic and evolutionary microbiology, 2006Co-Authors: Alejandro López-cortés, Guy Fauque, Marie-laure Fardeau, Catherine Joulian, Bernard OllivierAbstract:Desulfovibrio vulgaris subsp. oxamicus (type strain, DSM 1925(T)) was found to use nitrate as a terminal electron acceptor, the latter being reduced to ammonium. Phylogenetic studies indicated that strain DSM 1925(T) was distantly related to the type strain of Desulfovibrio vulgaris (95.4 % similarity of the small-subunit rRNA gene) and had as its closest phylogenetic relatives two other nitrate- and sulfate-reducing bacteria, namely Desulfovibrio termitidis (99.4 % similarity) and Desulfovibrio longreachensis (98.4 % similarity). Additional experiments were conducted to characterize better strain DSM 1925(T). This strain incompletely oxidized lactate and ethanol to acetate. It also oxidized butanol, pyruvate and citrate, but not glucose, fructose, acetate, propionate, butyrate, methanol, glycerol or peptone. The optimum temperature for growth was 37 degrees C (range 16-50 degrees C) and the optimum NaCl concentration for growth was 0.1 % (range 0-5 %). Because of significant genotypic and phenotypic differences from Desulfovibrio termitidis and Desulfovibrio longreachensis, reclassification of Desulfovibrio vulgaris subsp. oxamicus as Desulfovibrio oxamicus sp. nov., comb. nov., is proposed. The type strain is strain Monticello 2(T) (=DSM 1925(T)=NCIMB 9442(T)=ATCC 33405(T)).
Marie-laure Fardeau - One of the best experts on this subject based on the ideXlab platform.
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reclassification of the sulfate and nitrate reducing bacterium Desulfovibrio vulgaris subsp oxamicus as Desulfovibrio oxamicus sp nov comb nov
International Journal of Systematic and Evolutionary Microbiology, 2006Co-Authors: Alejandro Lopezcortes, Guy Fauque, Marie-laure Fardeau, Catherine Joulian, Bernard OllivierAbstract:Desulfovibrio vulgaris subsp. oxamicus (type strain, DSM 1925T) was found to use nitrate as a terminal electron acceptor, the latter being reduced to ammonium. Phylogenetic studies indicated that strain DSM 1925T was distantly related to the type strain of Desulfovibrio vulgaris (95.4 % similarity of the small-subunit rRNA gene) and had as its closest phylogenetic relatives two other nitrate- and sulfate-reducing bacteria, namely Desulfovibrio termitidis (99.4 % similarity) and Desulfovibrio longreachensis (98.4 % similarity). Additional experiments were conducted to characterize better strain DSM 1925T. This strain incompletely oxidized lactate and ethanol to acetate. It also oxidized butanol, pyruvate and citrate, but not glucose, fructose, acetate, propionate, butyrate, methanol, glycerol or peptone. The optimum temperature for growth was 37 °C (range 16–50 °C) and the optimum NaCl concentration for growth was 0.1 % (range 0–5 %). Because of significant genotypic and phenotypic differences from Desulfovibrio termitidis and Desulfovibrio longreachensis, reclassification of Desulfovibrio vulgaris subsp. oxamicus as Desulfovibrio oxamicus sp. nov., comb. nov., is proposed. The type strain is strain Monticello 2T (=DSM 1925T=NCIMB 9442T=ATCC 33405T).
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Reclassification of the sulfate- and nitrate-reducing bacterium Desulfovibrio vulgaris subsp. oxamicus as Desulfovibrio oxamicus sp. nov., comb. nov.
International journal of systematic and evolutionary microbiology, 2006Co-Authors: Alejandro López-cortés, Guy Fauque, Marie-laure Fardeau, Catherine Joulian, Bernard OllivierAbstract:Desulfovibrio vulgaris subsp. oxamicus (type strain, DSM 1925(T)) was found to use nitrate as a terminal electron acceptor, the latter being reduced to ammonium. Phylogenetic studies indicated that strain DSM 1925(T) was distantly related to the type strain of Desulfovibrio vulgaris (95.4 % similarity of the small-subunit rRNA gene) and had as its closest phylogenetic relatives two other nitrate- and sulfate-reducing bacteria, namely Desulfovibrio termitidis (99.4 % similarity) and Desulfovibrio longreachensis (98.4 % similarity). Additional experiments were conducted to characterize better strain DSM 1925(T). This strain incompletely oxidized lactate and ethanol to acetate. It also oxidized butanol, pyruvate and citrate, but not glucose, fructose, acetate, propionate, butyrate, methanol, glycerol or peptone. The optimum temperature for growth was 37 degrees C (range 16-50 degrees C) and the optimum NaCl concentration for growth was 0.1 % (range 0-5 %). Because of significant genotypic and phenotypic differences from Desulfovibrio termitidis and Desulfovibrio longreachensis, reclassification of Desulfovibrio vulgaris subsp. oxamicus as Desulfovibrio oxamicus sp. nov., comb. nov., is proposed. The type strain is strain Monticello 2(T) (=DSM 1925(T)=NCIMB 9442(T)=ATCC 33405(T)).
Catherine Joulian - One of the best experts on this subject based on the ideXlab platform.
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reclassification of the sulfate and nitrate reducing bacterium Desulfovibrio vulgaris subsp oxamicus as Desulfovibrio oxamicus sp nov comb nov
International Journal of Systematic and Evolutionary Microbiology, 2006Co-Authors: Alejandro Lopezcortes, Guy Fauque, Marie-laure Fardeau, Catherine Joulian, Bernard OllivierAbstract:Desulfovibrio vulgaris subsp. oxamicus (type strain, DSM 1925T) was found to use nitrate as a terminal electron acceptor, the latter being reduced to ammonium. Phylogenetic studies indicated that strain DSM 1925T was distantly related to the type strain of Desulfovibrio vulgaris (95.4 % similarity of the small-subunit rRNA gene) and had as its closest phylogenetic relatives two other nitrate- and sulfate-reducing bacteria, namely Desulfovibrio termitidis (99.4 % similarity) and Desulfovibrio longreachensis (98.4 % similarity). Additional experiments were conducted to characterize better strain DSM 1925T. This strain incompletely oxidized lactate and ethanol to acetate. It also oxidized butanol, pyruvate and citrate, but not glucose, fructose, acetate, propionate, butyrate, methanol, glycerol or peptone. The optimum temperature for growth was 37 °C (range 16–50 °C) and the optimum NaCl concentration for growth was 0.1 % (range 0–5 %). Because of significant genotypic and phenotypic differences from Desulfovibrio termitidis and Desulfovibrio longreachensis, reclassification of Desulfovibrio vulgaris subsp. oxamicus as Desulfovibrio oxamicus sp. nov., comb. nov., is proposed. The type strain is strain Monticello 2T (=DSM 1925T=NCIMB 9442T=ATCC 33405T).
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Reclassification of the sulfate- and nitrate-reducing bacterium Desulfovibrio vulgaris subsp. oxamicus as Desulfovibrio oxamicus sp. nov., comb. nov.
International journal of systematic and evolutionary microbiology, 2006Co-Authors: Alejandro López-cortés, Guy Fauque, Marie-laure Fardeau, Catherine Joulian, Bernard OllivierAbstract:Desulfovibrio vulgaris subsp. oxamicus (type strain, DSM 1925(T)) was found to use nitrate as a terminal electron acceptor, the latter being reduced to ammonium. Phylogenetic studies indicated that strain DSM 1925(T) was distantly related to the type strain of Desulfovibrio vulgaris (95.4 % similarity of the small-subunit rRNA gene) and had as its closest phylogenetic relatives two other nitrate- and sulfate-reducing bacteria, namely Desulfovibrio termitidis (99.4 % similarity) and Desulfovibrio longreachensis (98.4 % similarity). Additional experiments were conducted to characterize better strain DSM 1925(T). This strain incompletely oxidized lactate and ethanol to acetate. It also oxidized butanol, pyruvate and citrate, but not glucose, fructose, acetate, propionate, butyrate, methanol, glycerol or peptone. The optimum temperature for growth was 37 degrees C (range 16-50 degrees C) and the optimum NaCl concentration for growth was 0.1 % (range 0-5 %). Because of significant genotypic and phenotypic differences from Desulfovibrio termitidis and Desulfovibrio longreachensis, reclassification of Desulfovibrio vulgaris subsp. oxamicus as Desulfovibrio oxamicus sp. nov., comb. nov., is proposed. The type strain is strain Monticello 2(T) (=DSM 1925(T)=NCIMB 9442(T)=ATCC 33405(T)).
J.a. Cowan - One of the best experts on this subject based on the ideXlab platform.
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Functional Expression and Characterization of the Assimilatory-Type Sulfite Reductase from Desulfovibrio vulgaris (Hildenborough)
Archives of biochemistry and biophysics, 1994Co-Authors: J. Tan, A. Soriano, Siu Man Lui, J.a. CowanAbstract:By use of a conjugational transfer method, the gene encoding the assimilatory-type sulfite reductase (ASiR) from Desulfovibrio vulgaris (Hildenborough) has been functionally expressed in Desulfovibrio hosts using the broad-host-range plasmid pDSK519. Production is increased greater than 50-fold relative to natural expression levels. Recombinant enzyme has been characterized by standard biochemical methods, nuclear magnetic resonance, electron paramagnetic resonance, electronic absorption, and activity measurements. It cannot be distinguished from the native enzyme on the basis of spectroscopic characteristics or enzyme activity.
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Sequence and activity of an endogenous promoter of the assimilatory sulfite reductase gene from Desulfovibrio vulgaris (Hildenborough)
Biotechnology Letters, 1993Co-Authors: J. Tan, J.a. CowanAbstract:The 5′-flanking region of the assimilatory-type sulfite reductase-encoding gene from the anaerobic bacterium, Desulfovibrio vulgaris (Hildenborough), has been isolated and sequenced. The promoter element has been identified and compared with the consensus sequence for Escherichia coli and the sequences of promoter elements identified in other Desulfovibrio strains. The utility of such promoter sequences in E. coli-based expression systems is discussed.