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Karine Alain - One of the best experts on this subject based on the ideXlab platform.

Mohamed Jebbar - One of the best experts on this subject based on the ideXlab platform.

Alasdair M Cook - One of the best experts on this subject based on the ideXlab platform.

  • Taurine-Degrading, Non-Sulfate-Reducing Bacterium Bilophila wadsworthia RZATAU Contains a Fused DsrB-DsrD Subunit
    2016
    Co-Authors: Heike Laue, Michael Friedrich, Alasdair M Cook
    Abstract:

    A dissimilatory sulfite reductase (DSR) was purified from the anaerobic, taurine-degrading bacterium Bilo-phila wadsworthia RZATAU to apparent homogeneity. The enzyme is involved in energy conservation by re-ducing sulfite, which is formed during the degradation of taurine as an electron acceptor, to sulfide. Accord-ing to its UV-visible absorption spectrum with maxima at 392, 410, 583, and 630 nm, the enzyme belongs to the desulfoviridin type of DSRs. The sulfite reductase was isolated as an a2b2gn (n> 2) multimer with a native size of 285 kDa as determined by gel filtration. We have sequenced the genes encoding the a and b subunits (dsrA and dsrB, respectively), which probably constitute one operon. dsrA and dsrB encode polypeptides of 49 (a) and 54 kDa (b) which show significant similarities to the homologous subunits of other DSRs. The dsrB gene product of B. wadsworthia is apparently a fusion protein of dsrB and dsrD. This indicates a possible functional role of DsrD in DSR function because of its presence as a fusion protein as an integral part of the DSR holoenzyme in B. wadsworthia. A phylogenetic analysis using the available Dsr sequences revealed that B. wadsworthia grouped with its closest 16S rDNA relative Desulfovibrio desulfuricans Essex 6. Bilophila wadsworthia is a strictly anaerobic, gram-negative bacterium (2) which belongs to the family Desulfovibrionaceae in the delta subdivision of the Proteobacteria, but does no

  • dissimilatory sulfite reductase desulfoviridin of the taurine degrading non sulfate reducing bacterium bilophila wadsworthia rzatau contains a fused dsrb dsrd subunit
    Journal of Bacteriology, 2001
    Co-Authors: Heike Laue, Michael W Friedrich, Jurgen Ruff, Alasdair M Cook
    Abstract:

    A dissimilatory sulfite reductase (DSR) was purified from the anaerobic, taurine-degrading bacterium Bilophila wadsworthia RZATAU to apparent homogeneity. The enzyme is involved in energy conservation by reducing sulfite, which is formed during the degradation of taurine as an electron acceptor, to sulfide. According to its UV-visible absorption spectrum with maxima at 392, 410, 583, and 630 nm, the enzyme belongs to the desulfoviridin type of DSRs. The sulfite reductase was isolated as an a2b2gn (n > 2) multimer with a native size of 285 kDa as determined by gel filtration. We have sequenced the genes encoding the a and b subunits (dsrA and dsrB, respectively), which probably constitute one operon. dsrA and dsrB encode polypeptides of 49 (a) and 54 kDa (b) which show significant similarities to the homologous subunits of other DSRs. The dsrB gene product of B. wadsworthia is apparently a fusion protein of dsrB and dsrD. This indicates a possible functional role of DsrD in DSR function because of its presence as a fusion protein as an integral part of the DSR holoenzyme in B. wadsworthia. A phylogenetic analysis using the available Dsr sequences revealed that B. wadsworthia grouped with its closest 16S rDNA relative Desulfovibrio desulfuricans Essex 6. Bilophila wadsworthia is a strictly anaerobic, gram-negative bacterium (2) which belongs to the family Desulfovibrionaceae in the delta subdivision of the Proteobacteria, but does not

  • a Fused DsrB-DsrD Subunit
    2000
    Co-Authors: Heike Laue, Michael Friedrich, Jurgen Ruff, Alasdair M Cook
    Abstract:

    A dissimilatory sulfite reductase (DSR) was purified from the anaerobic, taurine-degrading bacterium Bilophila wadsworthia RZATAU to apparent homogeneity. The enzyme is involved in energy conservation by reducing sulfite, which is formed during the degradation of taurine as an electron acceptor, to sulfide. According to its UV-visible absorption spectrum with maxima at 392, 410, 583, and 630 nm, the enzyme belongs to the desulfoviridin type of DSRs. The sulfite reductase was isolated as an � 2 � 2 � n (n> 2) multimer with a native size of 285 kDa as determined by gel filtration. We have sequenced the genes encoding the � and � subunits (dsrA and dsrB, respectively), which probably constitute one operon. dsrA and dsrB encode polypeptides of 49 (�) and 54 kDa (�) which show significant similarities to the homologous subunits of other DSRs. The dsrB gene product of B. wadsworthia is apparently a fusion protein of dsrB and dsrD. This indicates a possible functional role of DsrD in DSR function because of its presence as a fusion protein as an integral part of the DSR holoenzyme in B. wadsworthia. A phylogenetic analysis using the available Dsr sequences revealed that B. wadsworthia grouped with its closest 16S rDNA relative Desulfovibrio desulfuricans Essex 6. Bilophila wadsworthia is a strictly anaerobic, gram-negative bacterium (2) which belongs to the family Desulfovibrionaceae in the delta subdivision of the Proteobacteria, but does no

Abdoulaye Thioye - One of the best experts on this subject based on the ideXlab platform.

  • desulfovibrio senegalensis sp nov a mesophilic sulfate reducer isolated from marine sediment
    International Journal of Systematic and Evolutionary Microbiology, 2017
    Co-Authors: Abdoulaye Thioye, Malick Mbengue, Manon Josephbartoli, Coumba Tourekane, Jean-luc Cayol, Marc Labat
    Abstract:

    Several strains of sulfate-reducing bacteria were isolated from marine sediments recovered from Hann Bay (Senegal). All were related to members of the genus Desulfovibrio . A strictly anaerobic, mesophilic and moderately halophilic strain designated BLaC1T was further characterized. Cells of strain BLaC1T stained Gram-negative and were 0.5 µm wide and 2–4 µm long, motile, rod-shaped and non-spore-forming. The four major fatty acids were anteiso-C15 : 0, iso-C15 : 0, iso-C17 : 0 and anteiso-C17 : 0. Growth was observed from 15 to 45 °C (optimum 40 °C) and at pH 5.5–8 (optimum pH 7.5). The salinity range for growth was 5–65 g NaCl l−1 (optimum 30 g l−1). Yeast extract was required for growth. Strain BLaC1T was able to grow on lactate and acetate in the presence of sulfate as an electron acceptor. Sulfate, thiosulfate and sulfite could serve as terminal electron acceptors, but not fumarate, nitrate or elemental sulfur. The DNA G+C content was 55.8 mol%. 16S rRNA gene sequence analysis assigned strain BLaC1T to the family Desulfovibrionaceae ; its closest relative was Desulfovibrio oxyclinae DSM 19275T (93.7 % similarity). On the basis of 16S rRNA gene sequence comparisons and physiological characteristics, strain BLaC1T is proposed as representing a novel species of Desulfovibrio , with the name Desulfovibrio senegalensis sp. nov. The type strain is BLaC1T (=DSM 101509T=JCM 31063T).

Jean-luc Cayol - One of the best experts on this subject based on the ideXlab platform.

  • desulfovibrio senegalensis sp nov a mesophilic sulfate reducer isolated from marine sediment
    International Journal of Systematic and Evolutionary Microbiology, 2017
    Co-Authors: Abdoulaye Thioye, Malick Mbengue, Manon Josephbartoli, Coumba Tourekane, Jean-luc Cayol, Marc Labat
    Abstract:

    Several strains of sulfate-reducing bacteria were isolated from marine sediments recovered from Hann Bay (Senegal). All were related to members of the genus Desulfovibrio . A strictly anaerobic, mesophilic and moderately halophilic strain designated BLaC1T was further characterized. Cells of strain BLaC1T stained Gram-negative and were 0.5 µm wide and 2–4 µm long, motile, rod-shaped and non-spore-forming. The four major fatty acids were anteiso-C15 : 0, iso-C15 : 0, iso-C17 : 0 and anteiso-C17 : 0. Growth was observed from 15 to 45 °C (optimum 40 °C) and at pH 5.5–8 (optimum pH 7.5). The salinity range for growth was 5–65 g NaCl l−1 (optimum 30 g l−1). Yeast extract was required for growth. Strain BLaC1T was able to grow on lactate and acetate in the presence of sulfate as an electron acceptor. Sulfate, thiosulfate and sulfite could serve as terminal electron acceptors, but not fumarate, nitrate or elemental sulfur. The DNA G+C content was 55.8 mol%. 16S rRNA gene sequence analysis assigned strain BLaC1T to the family Desulfovibrionaceae ; its closest relative was Desulfovibrio oxyclinae DSM 19275T (93.7 % similarity). On the basis of 16S rRNA gene sequence comparisons and physiological characteristics, strain BLaC1T is proposed as representing a novel species of Desulfovibrio , with the name Desulfovibrio senegalensis sp. nov. The type strain is BLaC1T (=DSM 101509T=JCM 31063T).

  • Desulfocurvus vexinensis gen. nov., sp. nov., a sulfate-reducing bacterium isolated from a deep subsurface aquifer.
    International Journal of Systematic and Evolutionary Microbiology, 2009
    Co-Authors: Nihel Klouche, Jean-luc Cayol, Odile Basso, Jean-francois Lascourreges, Pierre Thomas, Guy Fauque, Marie Laure Fardeau, Michel Magot
    Abstract:

    A novel anaerobic, chemo-organotrophic bacterium, designated VNs36(T), was isolated from a well that collected water from a deep saline aquifer used for underground gas storage at a depth of 830 m in the Paris Basin, France. Cells were curved motile rods or vibrios (3.0-5.0x0.5 microm). Strain VNs36(T) grew at temperatures between 20 and 50 degrees C (optimum 37 degrees C) and at pH values between 5.0 and 9.0 (optimum 6.9). It did not require salt for growth, but tolerated up to 20 g NaCl l(-1) (optimum 2 g l(-1)). In the presence of sulfate, strain VNs36(T) used lactate, formate and pyruvate as carbon and energy sources. The main fermentation products from lactate were acetate, H(2) and CO(2). Sulfate, thiosulfate and sulfite were used as electron acceptors, but not sulfur. The genomic DNA G+C content of strain VNs36(T) was 67.2 mol%. Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain VNs36(T) was affiliated with the family Desulfovibrionaceae within the class Deltaproteobacteria. On the basis of 16S rRNA gene sequence comparisons, DNA G+C content and the absence of desulfoviridin in cell extracts, it is proposed that strain VNs36(T) be assigned to a new genus, Desulfocurvus gen. nov., as a representative of a novel species, Desulfocurvus vexinensis sp. nov. The type species of this genus is Desulfocurvus vexinensis with the type strain VNs36(T) (=DSM 17965(T)=JCM 14038(T)).