The Experts below are selected from a list of 1053 Experts worldwide ranked by ideXlab platform
H F Merk - One of the best experts on this subject based on the ideXlab platform.
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Dexpanthenol modulates gene expression in skin wound healing in vivo
Skin Pharmacology and Physiology, 2012Co-Authors: Ruth Heise, Yvonne Marquardt, Katharina Czaja, C Skazik, K Sebastian, P Kurschat, B Denecke, S Ekanayakebohlig, K P Wilhelm, H F MerkAbstract:Topical application of Dexpanthenol is widely used in clinical practice for the improvement of wound healing. Previous in vitro experiments identified a stimulatory effect of pantothenate on migration, proliferation and gene regulation in cultured human dermal fibroblasts. To correlate these in vitro findings with the more complex in vivo situation of wound healing, a clinical trial was performed in which the Dexpanthenol-induced gene expression profile in punch biopsies of previously injured and Dexpanthenol-treated skin in comparison to placebo-treated skin was analyzed at the molecular level by Affymetrix® GeneChip analysis. Upregulation of IL-6, IL-1β, CYP1B1, CXCL1, CCL18 and KAP 4–2 gene expression and downregulation of psorasin mRNA and protein expression were identified in samples treated topically with Dexpanthenol. This in vivo study might provide new insight into the molecular mechanisms responsible for the effect of Dexpanthenol in wound healing and shows strong correlations to previous in vitro data using cultured dermal fibroblasts.
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allergic contact reaction to Dexpanthenol lymphocyte transformation test and evidence for microsomal dependent metabolism of the allergen
Contact Dermatitis, 1993Co-Authors: Carolyn Hahn, Stefani Rritzsche, Rainier Fritzsche, Rosemarie Schneider, H F MerkAbstract:: In a patient with contact dermatitis, Dexpanthenol was found to be the causative allergen. There was a positive reaction to Dexpanthenol on patch testing. Controls did not show any positive reactions to Dexpanthenol on patch testing. Additionally, an LTT was performed. After preincubation with Dexpanthenol-modified microsomes, we observed an increase in lymphocyte proliferation to Dexpanthenol, in comparison to Dexpanthenol without microsomes, suggesting that microsomal metabolism plays a role in the pathogenesis of Dexpanthenol sensitization, because microsomes are known to possess drug metabolizing enzymes such as cytochrome P450.
Jens M. Baron - One of the best experts on this subject based on the ideXlab platform.
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Dexpanthenol in wound healing after medical and cosmetic interventions postprocedure wound healing
Pharmaceuticals, 2020Co-Authors: Julian Gorski, Jens M. Baron, Ehrhardt Proksch, Daphne Schmid, Lei ZhangAbstract:With the availability of new technologies, the number of subjects undergoing medical and cosmetic interventions is increasing. Many procedures (e.g., ablative fractional laser treatment) resulting in superficial/minor wounds require appropriate aftercare to prevent complications in wound healing and poor cosmetic outcome. We review the published evidence of the usefulness of topical Dexpanthenol in postprocedure wound healing and the associated mechanisms of action at the molecular level. A search in the PubMed and Embase databases was performed to query the terms Dexpanthenol, panthenol, superficial wound, minor wound, wound healing, skin repair, and postprocedure. Search results were categorized as clinical trials and in vitro studies. In vitro and clinical studies provided evidence that topically applied Dexpanthenol promotes superficial and postprocedure wound healing. Latest findings confirmed that Dexpanthenol upregulates genes that are critical for the healing process. The gene expression data are of clinical relevance as evidenced by prospective clinical studies indicating that topical Dexpanthenol accelerates wound healing with rapid re-epithelialization and restoration of skin barrier function following skin injury. It can therefore be inferred that topical Dexpanthenol represents an appropriate and state-of-the-art treatment option for superficial postprocedure wounds, especially when applied early after the superficial skin damage.
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Characterization of a novel standardized human three-dimensional skin wound healing model using non-sequential fractional ultrapulsed CO2 laser treatments
Lasers in Surgery and Medicine, 2015Co-Authors: Yvonne Marquardt, Katharina Czaja, Timm Steiner, Claudia Skazik-voogt, Philipp M. Amann, Ruth Heise, Hans F. Merk, Jens M. BaronAbstract:BACKGROUND AND OBJECTIVE: At present, there is no standardized in vitro human skin model for wound healing. Therefore, our aim was to establish and characterize an in vitro/ex vivo three-dimensional (3D) wound healing model, which we employed to analyze the effects of Dexpanthenol on wound healing and gene regulation.\nMATERIALS AND METHODS: The novel human 3D skin wound healing model using scaffold and collagen 3D organotypic skin equivalents was irradiated with a non-sequential fractional ultrapulsed CO2 laser. These standardized injured full-thickness skin equivalents enable qRT-PCR, microarray, and histological studies analyzing the effect of topically or systemically applied compounds on skin wound healing.\nRESULTS: These human laser-irradiated skin models were found to be appropriate for in vitro wound healing analysis. Topical treatment of skin wounds with a 5% Dexpanthenol water-in-oil emulsion or two different 5% Dexpanthenol oil-in-water emulsions clearly enhanced wound closure compared to laser-irradiated untreated control models. To find out whether this positive effect is caused by the active substance Dexpanthenol, laser-irradiated skin models were cultured in calciumpantothenate containing medium (20 μg/ml) compared to skin equivalents cultured without calciumpantothenate. 3D models cultured in calciumpantothenate revealed considerably faster wound closure compared to the control models. Quantitative RT-PCR studies showed enhanced mRNA expression of MMP3, IL1α, keratin-associated protein 4-12 (KRTAP4-12), and decreased expression of S100A7 in laser-irradiated skin models cultured in medium containing calciumpantothenate.\nCONCLUSION: This novel standardized human 3D skin wound healing model proves useful for topical pharmacological studies on wound healing and reveals new insights into molecular mechanisms of Dexpanthenol-mediated effects on wound healing. In addition, these novel 3D model systems can be used to monitor ex vivo effects of various laser systems on gene expression and morphology of human skin. Lasers Surg. Med. © 2015 Wiley Periodicals, Inc.
Sasan Zaeri - One of the best experts on this subject based on the ideXlab platform.
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fabrication characterization and in vivo evaluation of Dexpanthenol sustained release nanofibers for wound healing
Polymer Testing, 2020Co-Authors: Maryam Najafiasl, Shahriar Osfouri, Reza Azin, Sasan ZaeriAbstract:Abstract In this study, wound dressings consisting of Dexpanthenol (Dex)-loaded electrospun nanofibers were fabricated using polyvinyl alcohol (PVA)/sodium alginate (SA), and chitosan as the core and the shell, respectively. Considering the remarkable properties of chitosan, it was used as a shell against drug release and to improve the thermal stability, and tensile strength of the scaffold. By comparing the thermogravimetric, and tensile strength results of nanofibers with and without shell, it was revealed that the presence of chitosan in the shell side could improve the thermal stability and increased the tensile strength by about three times. The isotherm models of Dexpanthenol release from the PVA/SA/Dex-CS scaffold was best described by the Langmuir model. Besides, Fourier transform infrared, scanning electron microscopy, and X-ray diffraction techniques were performed to characterize nanofibers. Furthermore, an in vivo investigation of a wound dressing with Dexpanthenol showed better healing compared to the wound dressings without Dexpanthenol.
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alginate based electrospun core shell nanofibers containing Dexpanthenol a good candidate for wound dressing
Journal of Drug Delivery Science and Technology, 2020Co-Authors: Maryam Najafiasl, Shahriar Osfouri, Reza Azin, Sasan ZaeriAbstract:Abstract The skin prevents infection and contamination entering the body, and wound dressings are one of the most serious tools in wound healing. In the present work, the biocompatibility and swelling tendency of nanofibers increased by adding alginates to a polymer solution is investigated. Glutaraldehyde was used in different methods to strengthen nanofibers, and it was found that a better cross-link was made from the combination of glutaraldehyde with the polymer solution before electrospinning. As the use of drug accelerates the healing process, Dexpanthenol was added to the polymeric composition of polyvinyl alcohol (PVA) and sodium alginate (SA) using a blending method. The resulting composition was then used as the core of the nanofibers, and drug release was controlled by different shells. The results showed that the presence of chitosan 1% (w/v) in the shell side of nanofibers helped better control the drug release. Also, the drug release from Dexpanthenol-loaded wound dressing followed the Fickian diffusion mechanism with the Korsmeyer-Peppas model. MTT analysis and cell culture indicated that Dexpanthenol-loaded PVA/SA/Triton-Chitosan nanofibers not only were nontoxic to the fibroblast cells but also appropriately affected the cellular attachment and morphology. It was revealed that PVA/SA/Triton-Chitosan nanofibers could be used for tissue engineering applications.
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Alginate-based electrospun core/shell nanofibers containing Dexpanthenol: A good candidate for wound dressing
Journal of Drug Delivery Science and Technology, 2020Co-Authors: Maryam Najafiasl, Shahriar Osfouri, Reza Azin, Sasan ZaeriAbstract:Abstract The skin prevents infection and contamination entering the body, and wound dressings are one of the most serious tools in wound healing. In the present work, the biocompatibility and swelling tendency of nanofibers increased by adding alginates to a polymer solution is investigated. Glutaraldehyde was used in different methods to strengthen nanofibers, and it was found that a better cross-link was made from the combination of glutaraldehyde with the polymer solution before electrospinning. As the use of drug accelerates the healing process, Dexpanthenol was added to the polymeric composition of polyvinyl alcohol (PVA) and sodium alginate (SA) using a blending method. The resulting composition was then used as the core of the nanofibers, and drug release was controlled by different shells. The results showed that the presence of chitosan 1% (w/v) in the shell side of nanofibers helped better control the drug release. Also, the drug release from Dexpanthenol-loaded wound dressing followed the Fickian diffusion mechanism with the Korsmeyer-Peppas model. MTT analysis and cell culture indicated that Dexpanthenol-loaded PVA/SA/Triton-Chitosan nanofibers not only were nontoxic to the fibroblast cells but also appropriately affected the cellular attachment and morphology. It was revealed that PVA/SA/Triton-Chitosan nanofibers could be used for tissue engineering applications.
Irene Tausch - One of the best experts on this subject based on the ideXlab platform.
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Topical Use of Dexpanthenol in Skin Disorders
American Journal of Clinical Dermatology, 2002Co-Authors: Fritz Ebner, Andreas Heller, Frank Rippke, Irene TauschAbstract:Pantothenic acid is essential to normal epithelial function. It is a component of coenzyme A, which serves as a cofactor for a variety of enzyme-catalyzed reactions that are important in the metabolism of carbohydrates, fatty acids, proteins, gluconeogenesis, sterols, steroid hormones, and porphyrins. The topical use of Dexpanthenol, the stable alcoholic analog of pantothenic acid, is based on good skin penetration and high local concentrations of Dexpanthenol when administered in an adequate vehicle, such as water-in-oil emulsions. Topical Dexpanthenol acts like a moisturizer, improving stratum corneum hydration, reducing transepidermal water loss and maintaining skin softness and elasticity. Activation of fibroblast proliferation, which is of relevance in wound healing, has been observed both in vitro and in vivo with Dexpanthenol. Accelerated re-epithelization in wound healing, monitored by means of the transepidermal water loss as an indicator of the intact epidermal barrier function, has also been seen. Dexpanthenol has been shown to have an anti-inflammatory effect on experimental ultraviolet-induced erythema. Beneficial effects of Dexpanthenol have been observed in patients who have undergone skin transplantation or scar treatment, or therapy for burn injuries and different dermatoses. The stimulation of epithelization, granulation and mitigation of itching were the most prominent effects of formulations containing Dexpanthenol. In double-blind placebo-controlled clinical trials, Dexpanthenol was evaluated for its efficacy in improving wound healing. Epidermal wounds treated with Dexpanthenol emulsion showed a reduction in erythema, and more elastic and solid tissue regeneration. Monitoring of transepidermal water loss showed a significant acceleration of epidermal regeneration as a result of Dexpanthenol therapy, as compared with the vehicle. In an irritation model, pretreatment with Dexpanthenol cream resulted in significantly less damage to the stratum corneum barrier, compared with no pretreatment. Adjuvant skin care with Dexpanthenol considerably improved the symptoms of skin irritation, such as dryness of the skin, roughness, scaling, pruritus, erythema, erosion/fissures, over 3 to 4 weeks. Usually, the topical administration of Dexpanthenol preparations is well tolerated, with minimal risk of skin irritancy or sensitization.
Yvonne Marquardt - One of the best experts on this subject based on the ideXlab platform.
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Characterization of a novel standardized human three-dimensional skin wound healing model using non-sequential fractional ultrapulsed CO2 laser treatments
Lasers in Surgery and Medicine, 2015Co-Authors: Yvonne Marquardt, Katharina Czaja, Timm Steiner, Claudia Skazik-voogt, Philipp M. Amann, Ruth Heise, Hans F. Merk, Jens M. BaronAbstract:BACKGROUND AND OBJECTIVE: At present, there is no standardized in vitro human skin model for wound healing. Therefore, our aim was to establish and characterize an in vitro/ex vivo three-dimensional (3D) wound healing model, which we employed to analyze the effects of Dexpanthenol on wound healing and gene regulation.\nMATERIALS AND METHODS: The novel human 3D skin wound healing model using scaffold and collagen 3D organotypic skin equivalents was irradiated with a non-sequential fractional ultrapulsed CO2 laser. These standardized injured full-thickness skin equivalents enable qRT-PCR, microarray, and histological studies analyzing the effect of topically or systemically applied compounds on skin wound healing.\nRESULTS: These human laser-irradiated skin models were found to be appropriate for in vitro wound healing analysis. Topical treatment of skin wounds with a 5% Dexpanthenol water-in-oil emulsion or two different 5% Dexpanthenol oil-in-water emulsions clearly enhanced wound closure compared to laser-irradiated untreated control models. To find out whether this positive effect is caused by the active substance Dexpanthenol, laser-irradiated skin models were cultured in calciumpantothenate containing medium (20 μg/ml) compared to skin equivalents cultured without calciumpantothenate. 3D models cultured in calciumpantothenate revealed considerably faster wound closure compared to the control models. Quantitative RT-PCR studies showed enhanced mRNA expression of MMP3, IL1α, keratin-associated protein 4-12 (KRTAP4-12), and decreased expression of S100A7 in laser-irradiated skin models cultured in medium containing calciumpantothenate.\nCONCLUSION: This novel standardized human 3D skin wound healing model proves useful for topical pharmacological studies on wound healing and reveals new insights into molecular mechanisms of Dexpanthenol-mediated effects on wound healing. In addition, these novel 3D model systems can be used to monitor ex vivo effects of various laser systems on gene expression and morphology of human skin. Lasers Surg. Med. © 2015 Wiley Periodicals, Inc.
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Dexpanthenol modulates gene expression in skin wound healing in vivo
Skin Pharmacology and Physiology, 2012Co-Authors: Ruth Heise, Yvonne Marquardt, Katharina Czaja, C Skazik, K Sebastian, P Kurschat, B Denecke, S Ekanayakebohlig, K P Wilhelm, H F MerkAbstract:Topical application of Dexpanthenol is widely used in clinical practice for the improvement of wound healing. Previous in vitro experiments identified a stimulatory effect of pantothenate on migration, proliferation and gene regulation in cultured human dermal fibroblasts. To correlate these in vitro findings with the more complex in vivo situation of wound healing, a clinical trial was performed in which the Dexpanthenol-induced gene expression profile in punch biopsies of previously injured and Dexpanthenol-treated skin in comparison to placebo-treated skin was analyzed at the molecular level by Affymetrix® GeneChip analysis. Upregulation of IL-6, IL-1β, CYP1B1, CXCL1, CCL18 and KAP 4–2 gene expression and downregulation of psorasin mRNA and protein expression were identified in samples treated topically with Dexpanthenol. This in vivo study might provide new insight into the molecular mechanisms responsible for the effect of Dexpanthenol in wound healing and shows strong correlations to previous in vitro data using cultured dermal fibroblasts.