The Experts below are selected from a list of 7602 Experts worldwide ranked by ideXlab platform
Gilbert Greub - One of the best experts on this subject based on the ideXlab platform.
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Applications of MALDI-TOF mass spectrometry in clinical Diagnostic Microbiology
FEMS Microbiology Reviews, 2012Co-Authors: Antony Croxatto, Guy Prod'hom, Gilbert GreubAbstract:Until recently, microbial identification in clinical Diagnostic laboratories has mainly relied on conventional phenotypic and gene sequencing identification techniques. The development of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) devices has revolutionized the routine identification of microorganisms in clinical Microbiology laboratories by introducing an easy, rapid, high throughput, low-cost, and efficient identification technique. This technology has been adapted to the constraint of clinical Diagnostic laboratories and has the potential to replace and/or complement conventional identification techniques for both bacterial and fungal strains. Using standardized procedures, the resolution of MALDI-TOF MS allows accurate identification at the species level of most Gram-positive and Gram-negative bacterial strains with the exception of a few difficult strains that require more attention and further development of the method. Similarly, the routine identification by MALDI-TOF MS of yeast isolates is reliable and much quicker than conventional techniques. Recent studies have shown that MALDI-TOF MS has also the potential to accurately identify filamentous fungi and dermatophytes, providing that specific standardized procedures are established for these microorganisms. Moreover, MALDI-TOF MS has been used successfully for microbial typing and identification at the subspecies level, demonstrating that this technology is a potential efficient tool for epidemiological studies and for taxonomical classification.
Antony Croxatto - One of the best experts on this subject based on the ideXlab platform.
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Applications of MALDI-TOF mass spectrometry in clinical Diagnostic Microbiology
FEMS Microbiology Reviews, 2012Co-Authors: Antony Croxatto, Guy Prod'hom, Gilbert GreubAbstract:Until recently, microbial identification in clinical Diagnostic laboratories has mainly relied on conventional phenotypic and gene sequencing identification techniques. The development of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) devices has revolutionized the routine identification of microorganisms in clinical Microbiology laboratories by introducing an easy, rapid, high throughput, low-cost, and efficient identification technique. This technology has been adapted to the constraint of clinical Diagnostic laboratories and has the potential to replace and/or complement conventional identification techniques for both bacterial and fungal strains. Using standardized procedures, the resolution of MALDI-TOF MS allows accurate identification at the species level of most Gram-positive and Gram-negative bacterial strains with the exception of a few difficult strains that require more attention and further development of the method. Similarly, the routine identification by MALDI-TOF MS of yeast isolates is reliable and much quicker than conventional techniques. Recent studies have shown that MALDI-TOF MS has also the potential to accurately identify filamentous fungi and dermatophytes, providing that specific standardized procedures are established for these microorganisms. Moreover, MALDI-TOF MS has been used successfully for microbial typing and identification at the subspecies level, demonstrating that this technology is a potential efficient tool for epidemiological studies and for taxonomical classification.
Luca Guardabassi - One of the best experts on this subject based on the ideXlab platform.
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Diagnostic Microbiology in veterinary dermatology present and future
Veterinary Dermatology, 2017Co-Authors: Luca Guardabassi, Peter Damborg, Ivonne Stamm, Peter A. Kopp, Els M. Broens, Pierre-louis ToutainAbstract:© 2017 The Authors. Veterinary Dermatology published by John Wiley & Sons Ltd on behalf of the ESVD and ACVD.
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Diagnostic Microbiology in veterinary dermatology: present and future
Veterinary Dermatology, 2017Co-Authors: Luca Guardabassi, Peter Damborg, Ivonne Stamm, Peter A. Kopp, Els M. Broens, Pierre-louis ToutainAbstract:Background - The Microbiology laboratory can be perceived as a service provider rather than an integral part of the healthcare team. Objectives - The aim of this review is to discuss the current challenges of providing a state-of-the-art Diagnostic veterinary Microbiology service including the identification (ID) and antimicrobial susceptibility testing (AST) of key pathogens in veterinary dermatology. Methods - The Study Group for Veterinary Microbiology (ESGVM) of the European Society of Clinical Microbiology and Infectious Diseases (ESCMID) identified scientific, technological, educational and regulatory issues impacting the predictive value of AST and the quality of the service offered by Microbiology laboratories. Results - The advent of mass spectrometry has significantly reduced the time required for ID of key pathogens such as Staphylococcus pseudintermedius. However, the turnaround time for validated AST methods has remained unchanged for many years. Beyond scientific and technological constraints, AST methods are not harmonized and clinical breakpoints for some antimicrobial drugs are either missing or inadequate. Small laboratories, including in-clinic laboratories, are usually not adequately equipped to run up-to-date clinical microbiologic Diagnostic tests. Conclusions and clinical importance - ESGVM recommends the use of laboratories employing mass spectrometry for ID and broth micro-dilution for AST, and offering assistance by expert microbiologists on pre- and post-analytical issues. Setting general standards for veterinary clinical Microbiology, promoting antimicrobial stewardship, and the development of new, validated and rapid Diagnostic methods, especially for AST, are among the missions of ESGVM.
Pierre-louis Toutain - One of the best experts on this subject based on the ideXlab platform.
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Diagnostic Microbiology in veterinary dermatology present and future
Veterinary Dermatology, 2017Co-Authors: Luca Guardabassi, Peter Damborg, Ivonne Stamm, Peter A. Kopp, Els M. Broens, Pierre-louis ToutainAbstract:© 2017 The Authors. Veterinary Dermatology published by John Wiley & Sons Ltd on behalf of the ESVD and ACVD.
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Diagnostic Microbiology in veterinary dermatology: present and future
Veterinary Dermatology, 2017Co-Authors: Luca Guardabassi, Peter Damborg, Ivonne Stamm, Peter A. Kopp, Els M. Broens, Pierre-louis ToutainAbstract:Background - The Microbiology laboratory can be perceived as a service provider rather than an integral part of the healthcare team. Objectives - The aim of this review is to discuss the current challenges of providing a state-of-the-art Diagnostic veterinary Microbiology service including the identification (ID) and antimicrobial susceptibility testing (AST) of key pathogens in veterinary dermatology. Methods - The Study Group for Veterinary Microbiology (ESGVM) of the European Society of Clinical Microbiology and Infectious Diseases (ESCMID) identified scientific, technological, educational and regulatory issues impacting the predictive value of AST and the quality of the service offered by Microbiology laboratories. Results - The advent of mass spectrometry has significantly reduced the time required for ID of key pathogens such as Staphylococcus pseudintermedius. However, the turnaround time for validated AST methods has remained unchanged for many years. Beyond scientific and technological constraints, AST methods are not harmonized and clinical breakpoints for some antimicrobial drugs are either missing or inadequate. Small laboratories, including in-clinic laboratories, are usually not adequately equipped to run up-to-date clinical microbiologic Diagnostic tests. Conclusions and clinical importance - ESGVM recommends the use of laboratories employing mass spectrometry for ID and broth micro-dilution for AST, and offering assistance by expert microbiologists on pre- and post-analytical issues. Setting general standards for veterinary clinical Microbiology, promoting antimicrobial stewardship, and the development of new, validated and rapid Diagnostic methods, especially for AST, are among the missions of ESGVM.
Gwendolyn L. Gilbert - One of the best experts on this subject based on the ideXlab platform.
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Evidence based Diagnostic Microbiology: has its time come?
Journal of Clinical Pathology, 2001Co-Authors: Vitali Sintchenko, Gwendolyn L. GilbertAbstract:The concept of evidence based practice (EBP) has evolved in response to technological advances and external pressure to increase efficiency and to contain the cost of health care.1 The ambitious aim of evidence based health care, and evidence based pathology in particular, is to advance clinical diagnosis and prognosis of disease through research and dissemination of new knowledge that meets high standards of critical review.2, 3 EBP requirements and principles have been reviewed elsewhere.2, 4 Importantly, the approaches to evidence based treatment are well established; however, evidence based diagnosis is much less developed. The purpose of this commentary is to outline the current status of EBP in Diagnostic Microbiology and to suggest some steps that pathologists can take to promote patient outcome oriented Microbiology. Pathologists have been overwhelmed in recent years by the seemingly relentless growth of requirements to improve the effectiveness and efficiency of laboratory practice. In addition, the Diagnostic Microbiology market is becoming increasingly sensitive to costs and customer requirements. Cost cutting has a considerable negative effect on Microbiology research and development. Adding fuel to this concern, there is an emerging view that pathology is a clinical service that has to adjust to the expectations of patients and health service providers. The focus of quality control has been shifting from zero defects to zero defections of referring physicians. Diagnostic Microbiology services are increasingly criticised for encouraging higher rates of testing without concomitant benefits. At the same time, in a recent systematic review of laboratory audits, 46% of Microbiology Diagnostic tests were found to be inappropriate.5 How often appropriate tests are not requested has not been studied systematically.5, 6 The role of the Microbiology laboratory in the diagnosis of mild infections (acute bronchitis, urinary tract infections, community acquired pneumonia, etc) has …
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The MicroScan WalkAway Diagnostic Microbiology system — An evaluation
Pathology, 1995Co-Authors: Alistair Mcgregor, Francesca Schio, Sheila Beaton, Valerie Boulton, Margaret Perman, Gwendolyn L. GilbertAbstract:SummaryThe MicroScan WalkAway is an automated bacterial identification and susceptibility testing system that has only recently been marketed in Australasia.We evaluated the performance of the instrument using MicroScan Rapid fluorescent panels to determine the identity and antibiotic susceptibilities of 100 Gram negative and 100 Gram positive organisms representing both common clinical isolates and selected organisms of interest.MicroScan results were compared with those obtained by conventional biochemical identification, and antibiotic susceptibility testing using agar dilution following the National Committee on Clinical Laboratory Standards guidelines.MicroScan and reference identifications were in agreement for 93% of Gram negative organisms. MicroScan results were available within 2 hrs. Additional tests were required to confirm the identity of 9 isolates but on only 2 occasions would a definitive identification been delayed beyond 24 hrs. Very major or major discrepancies were seen in 2% and minor...
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the microscan walkaway Diagnostic Microbiology system an evaluation
Pathology, 1995Co-Authors: Alistair Mcgregor, Francesca Schio, Sheila Beaton, Valerie Boulton, Margaret Perman, Gwendolyn L. GilbertAbstract:SummaryThe MicroScan WalkAway is an automated bacterial identification and susceptibility testing system that has only recently been marketed in Australasia.We evaluated the performance of the instrument using MicroScan Rapid fluorescent panels to determine the identity and antibiotic susceptibilities of 100 Gram negative and 100 Gram positive organisms representing both common clinical isolates and selected organisms of interest.MicroScan results were compared with those obtained by conventional biochemical identification, and antibiotic susceptibility testing using agar dilution following the National Committee on Clinical Laboratory Standards guidelines.MicroScan and reference identifications were in agreement for 93% of Gram negative organisms. MicroScan results were available within 2 hrs. Additional tests were required to confirm the identity of 9 isolates but on only 2 occasions would a definitive identification been delayed beyond 24 hrs. Very major or major discrepancies were seen in 2% and minor...