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Lukasz Stelinski - One of the best experts on this subject based on the ideXlab platform.
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A weevil sex pheromone serves as an attractant for its entomopathogenic nematode predators
Chemoecology, 2017Co-Authors: Monique J. Rivera, Xavier Martini, Ashot Khrimian, Lukasz StelinskiAbstract:Diaprepes abbreviatus is an invasive pest of citrus in the USA from the Caribbean. Entomopathogenic nematodes (EPNs) are used as biological control agents in citrus agroecosystems against D. abbreviatus . EPNs respond to herbivore-induced volatiles from citrus roots to assist in the location of insect hosts. Here, we investigated EPN response to the male-produced sex pheromone of D. abbreviatus . In the laboratory, we used two-choice tests to investigate the behavioral response of Steinernema Diaprepesi , Heterorhabditis indica , Steinernema riobrave , and the plant parasitic nematode, Tylenchulus semipenetrans , to the synthetic sex pheromone of D. abbreviatus , as well as its natural source, beetle frass. Nematodes were not attracted by volatiles of citrus plant origin or carvacrol, a non-pheromone volatile associated with beetle frass. S. Diaprepesi and H. indica were attracted to the frass and the pheromone of D. abbreviatus . The response of S. Diaprepesi to the pheromone was greater than that of H. indica at all doses tested; the greatest response from both species occurred at 0.12 and 1.2 ng of pheromone/μL of solvent. Deploying the pheromone in a citrus grove increased the mortality of caged Diaprepes larvae as compared to control larvae deployed with solvent alone. Also, more EPNs were found in the soil surrounding the pheromone-baited larvae than the surrounding controls.
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A weevil sex pheromone serves as an attractant for its entomopathogenic nematode predators
Chemoecology, 2017Co-Authors: Monique J. Rivera, Xavier Martini, Ashot Khrimian, Lukasz StelinskiAbstract:Diaprepes abbreviatus is an invasive pest of citrus in the USA from the Caribbean. Entomopathogenic nematodes (EPNs) are used as biological control agents in citrus agroecosystems against D. abbreviatus . EPNs respond to herbivore-induced volatiles from citrus roots to assist in the location of insect hosts. Here, we investigated EPN response to the male-produced sex pheromone of D. abbreviatus . In the laboratory, we used two-choice tests to investigate the behavioral response of Steinernema Diaprepesi , Heterorhabditis indica , Steinernema riobrave , and the plant parasitic nematode, Tylenchulus semipenetrans , to the synthetic sex pheromone of D. abbreviatus , as well as its natural source, beetle frass. Nematodes were not attracted by volatiles of citrus plant origin or carvacrol, a non-pheromone volatile associated with beetle frass. S. Diaprepesi and H. indica were attracted to the frass and the pheromone of D. abbreviatus . The response of S. Diaprepesi to the pheromone was greater than that of H. indica at all doses tested; the greatest response from both species occurred at 0.12 and 1.2 ng of pheromone/μL of solvent. Deploying the pheromone in a citrus grove increased the mortality of caged Diaprepes larvae as compared to control larvae deployed with solvent alone. Also, more EPNs were found in the soil surrounding the pheromone-baited larvae than the surrounding controls.
Stephen L. Lapointe - One of the best experts on this subject based on the ideXlab platform.
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Infection of the Diaprepes Root Weevil, Diaprepes abbreviatus (Coleoptera: Curculionidae) by an Iridovirus
2015Co-Authors: Stephen L. LapointeAbstract:The Diaprepes root weevil (DRW), Diaprepes abbreviatus (L.), is now regarded as a principal threat to sustainability of the citrus industry in Florida. Since its introduction into Florida in 1964, the weevil has spread throughout the citrus producing areas of peninsular Florida. Adult females oviposit on leaves. Neonate larvae fall to the ground and burrow into the soil where they feed on progressively larger roots as the larvae grow. Tre
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J Chem Ecol (2012) 38:408–417 DOI 10.1007/s10886-012-0096-8 Identification and Synthesis of a Male-Produced Pheromone
2012Co-Authors: Stephen L. Lapointe, Ashot Khrimian, Hans T. Alborn, Rocco T. Aless, Paul S. Robbins, Ales Svatos, Joseph C. Dickens, O Otálora-luna, Fatma Kaplan, Peter E. TealAbstract:# The Author(s) 2012. This article is published with open access at SpringerLink.com Abstract An unsaturated hydroxy-ester pheromone was isolated from the headspace and feces of male Diaprepes abbreviatus, identified, and synthesized. The pheromone, methyl (E)-3-(2-hydroxyethyl)-4-methyl-2-pentenoate, was discovered by gas chromatography-coupled electroantennogram detection (GC-EAD), and identified by gas chromatography– mass spectrometry (GC-MS) and nuclear magnetic resonance Electronic supplementary material The online version of this article (doi:10.1007/s10886-012-0096-8) contains supplementary material, which is available to authorized users
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Cuticular hydrocarbons on elytra of the Diaprepes root weevil Diaprepes abbreviatus (L.) (Coleoptera: Curculionidae)
Agricultural and Forest Entomology, 2004Co-Authors: Stephen L. Lapointe, Wayne B. Hunter, Rocco T. AlessandroAbstract:External gland openings and associated structures on the elytra of teneral and mature Diaprepes root weevils, Diaprepes abbreviatus (L.), were elucidated by scanning electron microscopy (SEM). 2 There were clear differences between teneral, callow adults and fully mature adults. In the field, teneral adults remain in the pupal chamber in the soil until sclerotization of the cuticle is complete or nearly so. 3 Phenotypic variation of the elytra in this species consists of varying patterns and coloration of scaled intervals between a variable number of raised ridges devoid of scales. In addition to being thinner and lighter in colour than fully mature adults, the elytra of teneral adults were devoid of waxy hydrocarbon secretions. 4 External gland openings at the base of each scale were observed on teneral elytra and mature elytra washed with methylene chloride. 5 SEM evidence to document the production of waxy filaments by these glands and partial characterization of these by gas chromatography and mass spectro- metry are presented.
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EFFECT OF DIETARY COPPER ON LARVAL DEVELOPMENT OF Diaprepes ABBREVIATUS (COLEOPTERA: CURCULIONIDAE)
Florida Entomologist, 2004Co-Authors: Stephen L. Lapointe, Albert A. Weathersbee, Hamed Doostdar, Richard T. MayerAbstract:Larvae of the Diaprepes root weevil, Diaprepes abbreviatus (L.), were reared from hatching on an artificial diet containing four concentrations of two copper compounds, cupric sulfate (CuSO4) or cupric hydroxide [Cu(OH)2]. Negative effects of copper on insect development were observed only for early instars. Survival of larvae from hatching to 4 weeks of age was significantly affected by the copper compounds compared with the artificial diet alone, and greater mortality was associated with CuSO4 compared with Cu(OH)2. The two compounds had equivalent effects on larval weight gain of early instars. Weight gain was negatively correlated with increasing copper concentration. No effect of copper was observed on late instars maintained on these diets beyond the initial 4 weeks. Larval and pupal period, weight gain, and survival of late instars were statistically similar. No effect on larval survival or weight gain was observed when copper solutions were applied at nonphytotoxic levels to two varieties of citrus rootstock. The potential for manipulating citrus tree copper content to control this pest is discussed.
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Iridovirus infection of cell cultures from the Diaprepes root weevil, Diaprepes abbreviatus.
Journal of Insect Science, 2003Co-Authors: Wayne B. Hunter, Stephen L. LapointeAbstract:We here report the development and viral infection of a Diaprepes root weevil cell culture. Embryonic tissues of the root weevil were used to establish cell cultures for use in screening viral pathogens as potential biological control agents. Tissues were seeded into a prepared solution of insect medium and kept at a temperature of 24 o C. The cell culture had primarily fibroblast-like morphology with some epithelial monolayers. Root weevil cells were successfully infected in vitro with a known insect virus, Invertebrate Iridescent Virus 6. Potential uses of insect cell cultures and insect viruses are discussed.
Monique J. Rivera - One of the best experts on this subject based on the ideXlab platform.
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A weevil sex pheromone serves as an attractant for its entomopathogenic nematode predators
Chemoecology, 2017Co-Authors: Monique J. Rivera, Xavier Martini, Ashot Khrimian, Lukasz StelinskiAbstract:Diaprepes abbreviatus is an invasive pest of citrus in the USA from the Caribbean. Entomopathogenic nematodes (EPNs) are used as biological control agents in citrus agroecosystems against D. abbreviatus . EPNs respond to herbivore-induced volatiles from citrus roots to assist in the location of insect hosts. Here, we investigated EPN response to the male-produced sex pheromone of D. abbreviatus . In the laboratory, we used two-choice tests to investigate the behavioral response of Steinernema Diaprepesi , Heterorhabditis indica , Steinernema riobrave , and the plant parasitic nematode, Tylenchulus semipenetrans , to the synthetic sex pheromone of D. abbreviatus , as well as its natural source, beetle frass. Nematodes were not attracted by volatiles of citrus plant origin or carvacrol, a non-pheromone volatile associated with beetle frass. S. Diaprepesi and H. indica were attracted to the frass and the pheromone of D. abbreviatus . The response of S. Diaprepesi to the pheromone was greater than that of H. indica at all doses tested; the greatest response from both species occurred at 0.12 and 1.2 ng of pheromone/μL of solvent. Deploying the pheromone in a citrus grove increased the mortality of caged Diaprepes larvae as compared to control larvae deployed with solvent alone. Also, more EPNs were found in the soil surrounding the pheromone-baited larvae than the surrounding controls.
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A weevil sex pheromone serves as an attractant for its entomopathogenic nematode predators
Chemoecology, 2017Co-Authors: Monique J. Rivera, Xavier Martini, Ashot Khrimian, Lukasz StelinskiAbstract:Diaprepes abbreviatus is an invasive pest of citrus in the USA from the Caribbean. Entomopathogenic nematodes (EPNs) are used as biological control agents in citrus agroecosystems against D. abbreviatus . EPNs respond to herbivore-induced volatiles from citrus roots to assist in the location of insect hosts. Here, we investigated EPN response to the male-produced sex pheromone of D. abbreviatus . In the laboratory, we used two-choice tests to investigate the behavioral response of Steinernema Diaprepesi , Heterorhabditis indica , Steinernema riobrave , and the plant parasitic nematode, Tylenchulus semipenetrans , to the synthetic sex pheromone of D. abbreviatus , as well as its natural source, beetle frass. Nematodes were not attracted by volatiles of citrus plant origin or carvacrol, a non-pheromone volatile associated with beetle frass. S. Diaprepesi and H. indica were attracted to the frass and the pheromone of D. abbreviatus . The response of S. Diaprepesi to the pheromone was greater than that of H. indica at all doses tested; the greatest response from both species occurred at 0.12 and 1.2 ng of pheromone/μL of solvent. Deploying the pheromone in a citrus grove increased the mortality of caged Diaprepes larvae as compared to control larvae deployed with solvent alone. Also, more EPNs were found in the soil surrounding the pheromone-baited larvae than the surrounding controls.
Ashot Khrimian - One of the best experts on this subject based on the ideXlab platform.
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A weevil sex pheromone serves as an attractant for its entomopathogenic nematode predators
Chemoecology, 2017Co-Authors: Monique J. Rivera, Xavier Martini, Ashot Khrimian, Lukasz StelinskiAbstract:Diaprepes abbreviatus is an invasive pest of citrus in the USA from the Caribbean. Entomopathogenic nematodes (EPNs) are used as biological control agents in citrus agroecosystems against D. abbreviatus . EPNs respond to herbivore-induced volatiles from citrus roots to assist in the location of insect hosts. Here, we investigated EPN response to the male-produced sex pheromone of D. abbreviatus . In the laboratory, we used two-choice tests to investigate the behavioral response of Steinernema Diaprepesi , Heterorhabditis indica , Steinernema riobrave , and the plant parasitic nematode, Tylenchulus semipenetrans , to the synthetic sex pheromone of D. abbreviatus , as well as its natural source, beetle frass. Nematodes were not attracted by volatiles of citrus plant origin or carvacrol, a non-pheromone volatile associated with beetle frass. S. Diaprepesi and H. indica were attracted to the frass and the pheromone of D. abbreviatus . The response of S. Diaprepesi to the pheromone was greater than that of H. indica at all doses tested; the greatest response from both species occurred at 0.12 and 1.2 ng of pheromone/μL of solvent. Deploying the pheromone in a citrus grove increased the mortality of caged Diaprepes larvae as compared to control larvae deployed with solvent alone. Also, more EPNs were found in the soil surrounding the pheromone-baited larvae than the surrounding controls.
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A weevil sex pheromone serves as an attractant for its entomopathogenic nematode predators
Chemoecology, 2017Co-Authors: Monique J. Rivera, Xavier Martini, Ashot Khrimian, Lukasz StelinskiAbstract:Diaprepes abbreviatus is an invasive pest of citrus in the USA from the Caribbean. Entomopathogenic nematodes (EPNs) are used as biological control agents in citrus agroecosystems against D. abbreviatus . EPNs respond to herbivore-induced volatiles from citrus roots to assist in the location of insect hosts. Here, we investigated EPN response to the male-produced sex pheromone of D. abbreviatus . In the laboratory, we used two-choice tests to investigate the behavioral response of Steinernema Diaprepesi , Heterorhabditis indica , Steinernema riobrave , and the plant parasitic nematode, Tylenchulus semipenetrans , to the synthetic sex pheromone of D. abbreviatus , as well as its natural source, beetle frass. Nematodes were not attracted by volatiles of citrus plant origin or carvacrol, a non-pheromone volatile associated with beetle frass. S. Diaprepesi and H. indica were attracted to the frass and the pheromone of D. abbreviatus . The response of S. Diaprepesi to the pheromone was greater than that of H. indica at all doses tested; the greatest response from both species occurred at 0.12 and 1.2 ng of pheromone/μL of solvent. Deploying the pheromone in a citrus grove increased the mortality of caged Diaprepes larvae as compared to control larvae deployed with solvent alone. Also, more EPNs were found in the soil surrounding the pheromone-baited larvae than the surrounding controls.
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J Chem Ecol (2012) 38:408–417 DOI 10.1007/s10886-012-0096-8 Identification and Synthesis of a Male-Produced Pheromone
2012Co-Authors: Stephen L. Lapointe, Ashot Khrimian, Hans T. Alborn, Rocco T. Aless, Paul S. Robbins, Ales Svatos, Joseph C. Dickens, O Otálora-luna, Fatma Kaplan, Peter E. TealAbstract:# The Author(s) 2012. This article is published with open access at SpringerLink.com Abstract An unsaturated hydroxy-ester pheromone was isolated from the headspace and feces of male Diaprepes abbreviatus, identified, and synthesized. The pheromone, methyl (E)-3-(2-hydroxyethyl)-4-methyl-2-pentenoate, was discovered by gas chromatography-coupled electroantennogram detection (GC-EAD), and identified by gas chromatography– mass spectrometry (GC-MS) and nuclear magnetic resonance Electronic supplementary material The online version of this article (doi:10.1007/s10886-012-0096-8) contains supplementary material, which is available to authorized users
Wayne B. Hunter - One of the best experts on this subject based on the ideXlab platform.
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Cuticular hydrocarbons on elytra of the Diaprepes root weevil Diaprepes abbreviatus (L.) (Coleoptera: Curculionidae)
Agricultural and Forest Entomology, 2004Co-Authors: Stephen L. Lapointe, Wayne B. Hunter, Rocco T. AlessandroAbstract:External gland openings and associated structures on the elytra of teneral and mature Diaprepes root weevils, Diaprepes abbreviatus (L.), were elucidated by scanning electron microscopy (SEM). 2 There were clear differences between teneral, callow adults and fully mature adults. In the field, teneral adults remain in the pupal chamber in the soil until sclerotization of the cuticle is complete or nearly so. 3 Phenotypic variation of the elytra in this species consists of varying patterns and coloration of scaled intervals between a variable number of raised ridges devoid of scales. In addition to being thinner and lighter in colour than fully mature adults, the elytra of teneral adults were devoid of waxy hydrocarbon secretions. 4 External gland openings at the base of each scale were observed on teneral elytra and mature elytra washed with methylene chloride. 5 SEM evidence to document the production of waxy filaments by these glands and partial characterization of these by gas chromatography and mass spectro- metry are presented.
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Iridovirus infection of cell cultures from the Diaprepes root weevil, Diaprepes abbreviatus.
Journal of Insect Science, 2003Co-Authors: Wayne B. Hunter, Stephen L. LapointeAbstract:We here report the development and viral infection of a Diaprepes root weevil cell culture. Embryonic tissues of the root weevil were used to establish cell cultures for use in screening viral pathogens as potential biological control agents. Tissues were seeded into a prepared solution of insect medium and kept at a temperature of 24 o C. The cell culture had primarily fibroblast-like morphology with some epithelial monolayers. Root weevil cells were successfully infected in vitro with a known insect virus, Invertebrate Iridescent Virus 6. Potential uses of insect cell cultures and insect viruses are discussed.
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toxicity and repellency of tephrosia candida to larval and adult Diaprepes root weevil coleoptera curculionidae
Journal of Economic Entomology, 2003Co-Authors: Stephen L. Lapointe, Cindy L Mckenzie, Wayne B. HunterAbstract:Abstract Leaves of the tropical legume Tephrosia candida DC deterred feeding by adults of the Diaprepes root weevil, Diaprepes abbreviatus (L.), compared with leaves of Citrus macrophylla Wester, a common citrus rootstock, or T. vogelii Hook. f. When larvae were placed in pots containing plants of the three species for 28 d in a growth chamber, larval survival and weight gain were significantly reduced in pots containing plants of T. candida compared with larvae in pots with C. macrophylla or T. vogelii. Diet incorporation of lyophilized roots of T. candida into an artificial diet increasingly inhibited the growth of larvae and increased larval mortality with increased concentration of roots, whereas roots from C. macrophylla or T. vogelii had no effect compared with the diet-only control. T. candida, but not T. vogelii, contains at least one constituent that acts as an antifeedant toward adult D. abbreviatus and as a toxicant toward larvae. No antifeedant effect of roots of T. candida toward larvae was o...
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Toxicity and Repellency of Tephrosia candida to Larval and Adult Diaprepes Root Weevil (Coleoptera: Curculionidae)
Journal of Economic Entomology, 2003Co-Authors: Stephen L. Lapointe, Cindy L Mckenzie, Wayne B. HunterAbstract:Leaves of the tropical legume Tephrosia candida DC deterred feeding by adults of the Diaprepes root weevil, Diaprepes abbreviatus (L.), compared with leaves of Citrus macrophylla Wester, a common citrus rootstock, or T. vogelii Hook. f. When larvae were placed in pots containing plants of the three species for 28 d in a growth chamber, larval survival and weight gain were significantly reduced in pots containing plants of T. candida compared with larvae in pots with C. macrophylla or T. vogelii. Diet incorporation of lyophilized roots of T. candida into an artificial diet increasingly inhibited the growth of larvae and increased larval mortality with increased concentration of roots, whereas roots from C. macrophylla or T. vogelii had no effect compared with the diet-only control. T. candida, but not T. vogelii, contains at least one constituent that acts as an antifeedant toward adult D. abbreviatus and as a toxicant toward larvae. No antifeedant effect of roots of T. candida toward larvae was observed in no-choice pot tests or in a diet incorporation bioassay. In pots, larval feeding damage to roots of T. candida was evident. In the diet incorporation assay, 97% of larvae survived 29 d on a diet of cellulose powder (a nutritionally inert filler) despite losing weight. We conclude that decreased survival and weight gain of larvae-fed fresh or lyophilized roots of T. candida were the result of ingestion of a toxicant and not deterrence from feeding.
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Iridovirus in the root weevil Diaprepes abbreviatus
Journal of Insect Science, 2003Co-Authors: Wayne B. Hunter, Stephen L. Lapointe, X. H. Sinisterra, D. S. Achor, C. J. FunkAbstract:Invertebrate iridescent virus 6 (IIV6) was evaluated for mode of transmission and ability to cause infection in the root weevil, Diaprepes abbreviatus (L.). This is the first evidence of IIV6 infection in D. abbreviatus, which caused both patent and sub-lethal covert infections in both larvae and adults. Adults and larvae were successfully infected with IIV6 by puncture, injection and per os. Transmission of IIV6 was demonstrated between infected and healthy individuals regardless of gender. Virus was detected in egg masses produced by virus-infected females suggesting IIV6 is transmitted transovarially. Virus particles were observed in the cytoplasm of weevil cells, and were shown to infect fat bodies, muscle, and nerve tissues, as visualized using transmission electron microscopy. Patent infections resulted in death of individuals within 3 to 4 days post infection. Individuals with covert infections tested positive for virus infection on day 7 by polymerase chain reaction analysis. Sequencing of PCR amplicons confirmed virus infection. Discovery of new pathogens against root weevils may provide new management tools for development of control strategies based on induced epizootics. This is the first report of a virus infecting D. abbreviatus.