The Experts below are selected from a list of 216 Experts worldwide ranked by ideXlab platform
Frantisek Baluska - One of the best experts on this subject based on the ideXlab platform.
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anaesthesia with Diethyl Ether impairs jasmonate signalling in the carnivorous plant venus flytrap dionaea muscipula
Annals of Botany, 2020Co-Authors: Andrej Pavlovic, Michaela Libiakova, Boris Bokor, Jana Jaksova, Ivan Petřik, Ondřej Novak, Frantisek BaluskaAbstract:BACKGROUND AND AIMS: General anaesthetics are compounds that induce loss of responsiveness to environmental stimuli in animals and humans. The primary site of action of general anaesthetics is the nervous system, where anaesthetics inhibit neuronal transmission. Although plants do not have neurons, they generate electrical signals in response to biotic and abiotic stresses. Here, we investigated the effect of the general volatile anaesthetic Diethyl Ether on the ability to sense potential prey or herbivore attacks in the carnivorous plant Venus flytrap (Dionaea muscipula). METHODS: We monitored trap movement, electrical signalling, phytohormone accumulation and gene expression in response to the mechanical stimulation of trigger hairs and wounding under Diethyl Ether treatment. KEY RESULTS: Diethyl Ether completely inhibited the generation of action potentials and trap closing reactions, which were easily and rapidly restored when the anaesthetic was removed. Diethyl Ether also inhibited the later response: jasmonic acid (JA) accumulation and expression of JA-responsive genes (cysteine protease dionain and type I chitinase). However, external application of JA bypassed the inhibited action potentials and restored gene expression under Diethyl Ether anaesthesia, indicating that downstream reactions from JA are not inhibited. CONCLUSIONS: The Venus flytrap cannot sense prey or a herbivore attack under Diethyl Ether treatment caused by inhibited action potentials, and the JA signalling pathway as a consequence.
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anaesthesia with Diethyl Ether impairs jasmonate signalling in the carnivorous plant venus flytrap dionaea muscipula
bioRxiv, 2019Co-Authors: Andrej Pavlovic, Michaela Libiakova, Boris Bokor, Jana Jaksova, Ivan Petřik, Ondřej Novak, Frantisek BaluskaAbstract:Summary General anaesthetics are compounds that induce loss of responsiveness to environmental stimuli in animals and humans. The primary site of general anaesthetic action is the nervous system, where anaesthetics inhibit neuronal transmission. Although plants do not have neurons, they generate electrical signals in response to biotic and abiotic stresses. Here, we investigated the effect of the general volatile anaesthetic Diethyl Ether on the ability to sense potential prey or herbivore attacks in the carnivorous plant Venus flytrap (Dionaea muscipula). We monitored trap movement, electrical signalling, phytohormone accumulation and gene expression in response to the mechanical stimulation of trigger hairs and wounding under Diethyl Ether treatment. Diethyl Ether completely inhibited the generation of action potentials and trap closing reactions, which were easily and rapidly restored when the anaesthetic was removed. Diethyl Ether also inhibited the later response: jasmonate (JA) accumulation and expression of JA-responsive genes. However, external application of JA bypassed the inhibited action potentials and restored gene expression under Diethyl Ether anaesthesia, indicating that downstream reactions from JA are not inhibited. Thus, the Venus flytrap cannot sense prey or a herbivore attack under Diethyl Ether treatment. This is an intriguing parallel to the effect of anaesthesia on animals and humans. Highlight Carnivorous plant Venus flytrap (Dionaea muscipula) is unresponsive to insect prey or herbivore attack due to impaired electrical and jasmonate signalling under general anaesthesia induced by Diethyl Ether.
Guido Busca - One of the best experts on this subject based on the ideXlab platform.
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ethanol dehydration on silica aluminas active sites and ethylene Diethyl Ether selectivities
Catalysis Communications, 2015Co-Authors: Thanh Khoa Phung, Guido BuscaAbstract:Abstract Commercial silica-alumina catalysts prepared by different procedures have been characterized. Both present strong Lewis acidity togEther with Bronsted sites able to protonate pyridine. No evidence of “zeolitic” bridging OH's but significant heterogeneity of terminal silanol groups, part of which are likely “pseudobridging”, was found. Similar high activity in ethanol conversion but markedly different selectivities to ethylene and Diethyl Ether were found. They are less active than both zeolites and γ-Al 2 O 3 . Lewis sites with alumina-like acidobasic neighbor are more selective for ethylene production while Lewis sites with silica-like covalent neighbor are more selective for Diethyl Ether.
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Ethanol dehydration on silica-aluminas: Active sites and ethylene/Diethyl Ether selectivities
Catalysis Communications, 2015Co-Authors: Thanh Khoa Phung, Guido BuscaAbstract:Commercial silica-alumina catalysts prepared by different procedures have been characterized. Both present strong Lewis acidity togEther with Brønsted sites able to protonate pyridine. No evidence of "zeolitic" bridging OH's but significant heterogeneity of terminal silanol groups, part of which are likely "pseudobridging", was found. Similar high activity in ethanol conversion but markedly different selectivities to ethylene and Diethyl Ether were found. They are less active than both zeolites and γ-Al2O3. Lewis sites with alumina-like acidobasic neighbor are more selective for ethylene production while Lewis sites with silica-like covalent neighbor are more selective for Diethyl Ether.
Dionisios G Vlachos - One of the best experts on this subject based on the ideXlab platform.
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density functional theory computed mechanisms of ethylene and Diethyl Ether formation from ethanol on γ al2o3 100
ACS Catalysis, 2013Co-Authors: Matthew A. Christiansen, Giannis Mpourmpakis, Dionisios G VlachosAbstract:Multiple potential active sites on the surface of γ-Al2O3 have led to debate about the role of Lewis and/or Bronsted acidity in reactions of ethanol, while mechanistic insights into competitive production of ethylene and Diethyl Ether are scarce. In this study, elementary adsorption and reaction mechanisms for ethanol dehydration and Etherification are studied on the γ-Al2O3(100) surface using density functional theory calculations. The O atom of adsorbed ethanol interacts strongly with surface Al (Lewis acid) sites, while adsorption is weak on Bronsted (surface H) and surface O sites. Water, a byproduct of both ethylene and Diethyl Ether formation, competes with ethanol for adsorption sites. Multiple pathways for ethylene formation from ethanol are explored, and a concerted Lewis-catalyzed elimination (E2) mechanism is found to be the energetically preferred pathway, with a barrier of Ea = 37 kcal/mol at the most stable site. Diethyl Ether formation mechanisms presented for the first time on γ-Al2O3 indi...
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Density functional theory-computed mechanisms of ethylene and Diethyl Ether formation from ethanol on γ-Al2O3(100)
ACS Catalysis, 2013Co-Authors: Matthew A. Christiansen, Giannis Mpourmpakis, Dionisios G VlachosAbstract:Multiple potential active sites on the surface of γ-Al2O3 have led to debate about the role of Lewis and/or Brønsted acidity in reactions of ethanol, while mechanistic insights into competitive production of ethylene and Diethyl Ether are scarce. In this study, elementary adsorption and reaction mechanisms for ethanol dehydration and Etherification are studied on the γ-Al2O3(100) surface using density functional theory calculations. The O atom of adsorbed ethanol interacts strongly with surface Al (Lewis acid) sites, while adsorption is weak on Brønsted (surface H) and surface O sites. Water, a byproduct of both ethylene and Diethyl Ether formation, competes with ethanol for adsorption sites. Multiple pathways for ethylene formation from ethanol are explored, and a concerted Lewis-catalyzed elimination (E2) mechanism is found to be the energetically preferred pathway, with a barrier of Ea = 37 kcal/mol at the most stable site. Diethyl Ether formation mechanisms presented for the first time on γ-Al2O3 indicate that the most favorable pathways involve Lewis-catalyzed SN2 reactions (Ea = 35 kcal/mol). Additional novel mechanisms for Diethyl Ether decomposition to ethylene are reported. Brønsted-catalyzed mechanisms for ethylene and Ether formation are not favorable on the (100) facet because of weak adsorption on Brønsted sites. These results explain multiple experimental observations, including the competition between ethylene and Diethyl Ether formation on alumina surfaces.
Andrej Pavlovic - One of the best experts on this subject based on the ideXlab platform.
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anaesthesia with Diethyl Ether impairs jasmonate signalling in the carnivorous plant venus flytrap dionaea muscipula
Annals of Botany, 2020Co-Authors: Andrej Pavlovic, Michaela Libiakova, Boris Bokor, Jana Jaksova, Ivan Petřik, Ondřej Novak, Frantisek BaluskaAbstract:BACKGROUND AND AIMS: General anaesthetics are compounds that induce loss of responsiveness to environmental stimuli in animals and humans. The primary site of action of general anaesthetics is the nervous system, where anaesthetics inhibit neuronal transmission. Although plants do not have neurons, they generate electrical signals in response to biotic and abiotic stresses. Here, we investigated the effect of the general volatile anaesthetic Diethyl Ether on the ability to sense potential prey or herbivore attacks in the carnivorous plant Venus flytrap (Dionaea muscipula). METHODS: We monitored trap movement, electrical signalling, phytohormone accumulation and gene expression in response to the mechanical stimulation of trigger hairs and wounding under Diethyl Ether treatment. KEY RESULTS: Diethyl Ether completely inhibited the generation of action potentials and trap closing reactions, which were easily and rapidly restored when the anaesthetic was removed. Diethyl Ether also inhibited the later response: jasmonic acid (JA) accumulation and expression of JA-responsive genes (cysteine protease dionain and type I chitinase). However, external application of JA bypassed the inhibited action potentials and restored gene expression under Diethyl Ether anaesthesia, indicating that downstream reactions from JA are not inhibited. CONCLUSIONS: The Venus flytrap cannot sense prey or a herbivore attack under Diethyl Ether treatment caused by inhibited action potentials, and the JA signalling pathway as a consequence.
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anaesthesia with Diethyl Ether impairs jasmonate signalling in the carnivorous plant venus flytrap dionaea muscipula
bioRxiv, 2019Co-Authors: Andrej Pavlovic, Michaela Libiakova, Boris Bokor, Jana Jaksova, Ivan Petřik, Ondřej Novak, Frantisek BaluskaAbstract:Summary General anaesthetics are compounds that induce loss of responsiveness to environmental stimuli in animals and humans. The primary site of general anaesthetic action is the nervous system, where anaesthetics inhibit neuronal transmission. Although plants do not have neurons, they generate electrical signals in response to biotic and abiotic stresses. Here, we investigated the effect of the general volatile anaesthetic Diethyl Ether on the ability to sense potential prey or herbivore attacks in the carnivorous plant Venus flytrap (Dionaea muscipula). We monitored trap movement, electrical signalling, phytohormone accumulation and gene expression in response to the mechanical stimulation of trigger hairs and wounding under Diethyl Ether treatment. Diethyl Ether completely inhibited the generation of action potentials and trap closing reactions, which were easily and rapidly restored when the anaesthetic was removed. Diethyl Ether also inhibited the later response: jasmonate (JA) accumulation and expression of JA-responsive genes. However, external application of JA bypassed the inhibited action potentials and restored gene expression under Diethyl Ether anaesthesia, indicating that downstream reactions from JA are not inhibited. Thus, the Venus flytrap cannot sense prey or a herbivore attack under Diethyl Ether treatment. This is an intriguing parallel to the effect of anaesthesia on animals and humans. Highlight Carnivorous plant Venus flytrap (Dionaea muscipula) is unresponsive to insect prey or herbivore attack due to impaired electrical and jasmonate signalling under general anaesthesia induced by Diethyl Ether.
Thanh Khoa Phung - One of the best experts on this subject based on the ideXlab platform.
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ethanol dehydration on silica aluminas active sites and ethylene Diethyl Ether selectivities
Catalysis Communications, 2015Co-Authors: Thanh Khoa Phung, Guido BuscaAbstract:Abstract Commercial silica-alumina catalysts prepared by different procedures have been characterized. Both present strong Lewis acidity togEther with Bronsted sites able to protonate pyridine. No evidence of “zeolitic” bridging OH's but significant heterogeneity of terminal silanol groups, part of which are likely “pseudobridging”, was found. Similar high activity in ethanol conversion but markedly different selectivities to ethylene and Diethyl Ether were found. They are less active than both zeolites and γ-Al 2 O 3 . Lewis sites with alumina-like acidobasic neighbor are more selective for ethylene production while Lewis sites with silica-like covalent neighbor are more selective for Diethyl Ether.
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Ethanol dehydration on silica-aluminas: Active sites and ethylene/Diethyl Ether selectivities
Catalysis Communications, 2015Co-Authors: Thanh Khoa Phung, Guido BuscaAbstract:Commercial silica-alumina catalysts prepared by different procedures have been characterized. Both present strong Lewis acidity togEther with Brønsted sites able to protonate pyridine. No evidence of "zeolitic" bridging OH's but significant heterogeneity of terminal silanol groups, part of which are likely "pseudobridging", was found. Similar high activity in ethanol conversion but markedly different selectivities to ethylene and Diethyl Ether were found. They are less active than both zeolites and γ-Al2O3. Lewis sites with alumina-like acidobasic neighbor are more selective for ethylene production while Lewis sites with silica-like covalent neighbor are more selective for Diethyl Ether.