The Experts below are selected from a list of 31815 Experts worldwide ranked by ideXlab platform
Galia Oron - One of the best experts on this subject based on the ideXlab platform.
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erratum to expression of growth Differentiating Factor 9 and its type 1 receptor in human ovaries reprod biomed online 21 2010 109 117
Reproductive Biomedicine Online, 2011Co-Authors: Galia Oron, Benjamin Fisch, Xiao Yun Zhang, Jacob Farhi, Avi Benharoush, Gania KesselericeksonAbstract:a Infertility and IVF Unit, Helen Schneider Hospital for Women, Rabin Medical Center, Beilinson Hospital, Petach Tikva 49100, Israel; b Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel; c Department of Obstetrics and Gynecology, Royal Victoria Hospital, McGill University, Montreal, Quebec, Canada; d Department of Human Genetics, McGill University, Montreal, Quebec, Canada; e Felsenstein Medical Research Center, Beilinson Hospital, Petach Tikva 49100, Israel * Corresponding author. E-mail address: ronita@clalit.org.il (R Abir).
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expression of growth Differentiating Factor 9 and its type 1 receptor in human ovaries
Reproductive Biomedicine Online, 2010Co-Authors: Galia Oron, Benjamin Fisch, Xiao Yun Zhang, Jacob Farhi, Avi Ben Haroush, Gania KesselericeksonAbstract:The expression of growth-Differentiating Factor 9 (GDF9) has not been studied in ovaries from girls and human fetuses nor has its receptor transforming growth Factor-beta1 receptor (TGFbetaR1) been investigated in ovaries of girls/women. The aim of this study was to fill these gaps. Ovarian samples were obtained from 16 human fetuses at 21-35 gestational weeks and from 34 girls/women aged 5-39years. These specimens were prepared for immunohistochemical staining of the GDF9 and TGFbetaR1 proteins. Reverse transcription polymerase chain reaction was used to detect GDF9 mRNA transcripts and in-situ hybridization to localize TGFbetaR1 mRNA transcripts. Positive staining for the GDF9 protein was identified in oocytes and granulosa cells in all samples tested. GDF9 mRNA transcripts were present in all samples. Protein expression of TGFbetaR1 was identified in granulosa cells in all samples. Oocyte staining was identified in samples from girls/women but in only one fetal sample. TGFbetaR1 mRNA transcripts were identified in granulosa cells and oocytes in 50% of the samples from fetuses aged over 22 gestational weeks and in samples from girls/women. The detection of GDF9 and TGFbetaR1 at both at the protein and mRNA levels suggests that GDF9 may have functions in human preantral follicles.
Benjamin Fisch - One of the best experts on this subject based on the ideXlab platform.
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growth Differentiating Factor 9 gdf9 and bone morphogenetic protein 15 both activate development of human primordial follicles in vitro with seemingly more beneficial effects of gdf9
The Journal of Clinical Endocrinology and Metabolism, 2011Co-Authors: Alon Kedem, Benjamin Fisch, Roni Garor, Adi Benzaken, Taya Gizunterman, Carmela Felz, Avi Benharoush, Dragan Kravarusic, Ronit AbirAbstract:Context: The signals initiating growth of primordial follicles are unknown. Bone morphogenetic protein 15 (BMP15) and growth Differentiating Factor 9 (GDF9) are promising candidates. Objective: The objective of the study was to evaluate for the first time the effects of human recombinant BMP15 and human recombinant GDF9 on the in vitro development of human primordial follicles. Design and Setting: This was a controlled culture study performed in a major tertiary university-affiliated medical center. Materials: Materials included ovarian tissue from 17 girls/women and three aborted human fetuses. Intervention: There were no interventions. Main Outcome Measure: Histological and immunohistochemical (proliferating cell nuclear antigen, BMP15, and GDF9) studies and an endocrine assay of 17β-estradiol were conducted. Results: In the samples from girls/women, the number of developing follicles was greater with GDF9 or BMP15 alone than with no BMP15 or GDF9. Higher 17β-estradiol secretion was noted after treatmen...
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erratum to expression of growth Differentiating Factor 9 and its type 1 receptor in human ovaries reprod biomed online 21 2010 109 117
Reproductive Biomedicine Online, 2011Co-Authors: Galia Oron, Benjamin Fisch, Xiao Yun Zhang, Jacob Farhi, Avi Benharoush, Gania KesselericeksonAbstract:a Infertility and IVF Unit, Helen Schneider Hospital for Women, Rabin Medical Center, Beilinson Hospital, Petach Tikva 49100, Israel; b Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel; c Department of Obstetrics and Gynecology, Royal Victoria Hospital, McGill University, Montreal, Quebec, Canada; d Department of Human Genetics, McGill University, Montreal, Quebec, Canada; e Felsenstein Medical Research Center, Beilinson Hospital, Petach Tikva 49100, Israel * Corresponding author. E-mail address: ronita@clalit.org.il (R Abir).
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expression of growth Differentiating Factor 9 and its type 1 receptor in human ovaries
Reproductive Biomedicine Online, 2010Co-Authors: Galia Oron, Benjamin Fisch, Xiao Yun Zhang, Jacob Farhi, Avi Ben Haroush, Gania KesselericeksonAbstract:The expression of growth-Differentiating Factor 9 (GDF9) has not been studied in ovaries from girls and human fetuses nor has its receptor transforming growth Factor-beta1 receptor (TGFbetaR1) been investigated in ovaries of girls/women. The aim of this study was to fill these gaps. Ovarian samples were obtained from 16 human fetuses at 21-35 gestational weeks and from 34 girls/women aged 5-39years. These specimens were prepared for immunohistochemical staining of the GDF9 and TGFbetaR1 proteins. Reverse transcription polymerase chain reaction was used to detect GDF9 mRNA transcripts and in-situ hybridization to localize TGFbetaR1 mRNA transcripts. Positive staining for the GDF9 protein was identified in oocytes and granulosa cells in all samples tested. GDF9 mRNA transcripts were present in all samples. Protein expression of TGFbetaR1 was identified in granulosa cells in all samples. Oocyte staining was identified in samples from girls/women but in only one fetal sample. TGFbetaR1 mRNA transcripts were identified in granulosa cells and oocytes in 50% of the samples from fetuses aged over 22 gestational weeks and in samples from girls/women. The detection of GDF9 and TGFbetaR1 at both at the protein and mRNA levels suggests that GDF9 may have functions in human preantral follicles.
Gania Kesselericekson - One of the best experts on this subject based on the ideXlab platform.
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erratum to expression of growth Differentiating Factor 9 and its type 1 receptor in human ovaries reprod biomed online 21 2010 109 117
Reproductive Biomedicine Online, 2011Co-Authors: Galia Oron, Benjamin Fisch, Xiao Yun Zhang, Jacob Farhi, Avi Benharoush, Gania KesselericeksonAbstract:a Infertility and IVF Unit, Helen Schneider Hospital for Women, Rabin Medical Center, Beilinson Hospital, Petach Tikva 49100, Israel; b Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel; c Department of Obstetrics and Gynecology, Royal Victoria Hospital, McGill University, Montreal, Quebec, Canada; d Department of Human Genetics, McGill University, Montreal, Quebec, Canada; e Felsenstein Medical Research Center, Beilinson Hospital, Petach Tikva 49100, Israel * Corresponding author. E-mail address: ronita@clalit.org.il (R Abir).
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expression of growth Differentiating Factor 9 and its type 1 receptor in human ovaries
Reproductive Biomedicine Online, 2010Co-Authors: Galia Oron, Benjamin Fisch, Xiao Yun Zhang, Jacob Farhi, Avi Ben Haroush, Gania KesselericeksonAbstract:The expression of growth-Differentiating Factor 9 (GDF9) has not been studied in ovaries from girls and human fetuses nor has its receptor transforming growth Factor-beta1 receptor (TGFbetaR1) been investigated in ovaries of girls/women. The aim of this study was to fill these gaps. Ovarian samples were obtained from 16 human fetuses at 21-35 gestational weeks and from 34 girls/women aged 5-39years. These specimens were prepared for immunohistochemical staining of the GDF9 and TGFbetaR1 proteins. Reverse transcription polymerase chain reaction was used to detect GDF9 mRNA transcripts and in-situ hybridization to localize TGFbetaR1 mRNA transcripts. Positive staining for the GDF9 protein was identified in oocytes and granulosa cells in all samples tested. GDF9 mRNA transcripts were present in all samples. Protein expression of TGFbetaR1 was identified in granulosa cells in all samples. Oocyte staining was identified in samples from girls/women but in only one fetal sample. TGFbetaR1 mRNA transcripts were identified in granulosa cells and oocytes in 50% of the samples from fetuses aged over 22 gestational weeks and in samples from girls/women. The detection of GDF9 and TGFbetaR1 at both at the protein and mRNA levels suggests that GDF9 may have functions in human preantral follicles.
Jacob Farhi - One of the best experts on this subject based on the ideXlab platform.
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erratum to expression of growth Differentiating Factor 9 and its type 1 receptor in human ovaries reprod biomed online 21 2010 109 117
Reproductive Biomedicine Online, 2011Co-Authors: Galia Oron, Benjamin Fisch, Xiao Yun Zhang, Jacob Farhi, Avi Benharoush, Gania KesselericeksonAbstract:a Infertility and IVF Unit, Helen Schneider Hospital for Women, Rabin Medical Center, Beilinson Hospital, Petach Tikva 49100, Israel; b Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel; c Department of Obstetrics and Gynecology, Royal Victoria Hospital, McGill University, Montreal, Quebec, Canada; d Department of Human Genetics, McGill University, Montreal, Quebec, Canada; e Felsenstein Medical Research Center, Beilinson Hospital, Petach Tikva 49100, Israel * Corresponding author. E-mail address: ronita@clalit.org.il (R Abir).
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expression of growth Differentiating Factor 9 and its type 1 receptor in human ovaries
Reproductive Biomedicine Online, 2010Co-Authors: Galia Oron, Benjamin Fisch, Xiao Yun Zhang, Jacob Farhi, Avi Ben Haroush, Gania KesselericeksonAbstract:The expression of growth-Differentiating Factor 9 (GDF9) has not been studied in ovaries from girls and human fetuses nor has its receptor transforming growth Factor-beta1 receptor (TGFbetaR1) been investigated in ovaries of girls/women. The aim of this study was to fill these gaps. Ovarian samples were obtained from 16 human fetuses at 21-35 gestational weeks and from 34 girls/women aged 5-39years. These specimens were prepared for immunohistochemical staining of the GDF9 and TGFbetaR1 proteins. Reverse transcription polymerase chain reaction was used to detect GDF9 mRNA transcripts and in-situ hybridization to localize TGFbetaR1 mRNA transcripts. Positive staining for the GDF9 protein was identified in oocytes and granulosa cells in all samples tested. GDF9 mRNA transcripts were present in all samples. Protein expression of TGFbetaR1 was identified in granulosa cells in all samples. Oocyte staining was identified in samples from girls/women but in only one fetal sample. TGFbetaR1 mRNA transcripts were identified in granulosa cells and oocytes in 50% of the samples from fetuses aged over 22 gestational weeks and in samples from girls/women. The detection of GDF9 and TGFbetaR1 at both at the protein and mRNA levels suggests that GDF9 may have functions in human preantral follicles.
Masahiro Shirakawa - One of the best experts on this subject based on the ideXlab platform.
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^1H, ^13C, and ^15N resonance assignment of the TIR domain of human MyD88
Biomolecular NMR Assignments, 2010Co-Authors: Hidenori Ohnishi, Hidehito Tochio, Zenichiro Kato, Takeshi Kimura, Hidekazu Hiroaki, Naomi Kondo, Masahiro ShirakawaAbstract:Myeloid Differentiating Factor 88 (MyD88) is one of a critical adaptor molecule in the Toll-like receptor (TLR) signaling pathway. The TIR domain of MyD88 serves as a protein–protein interaction module and interacts with other TIR-containing proteins such as Mal (MyD88 adaptor-like) and Toll-like receptor 4 to form signal initiation complexes. Here we report the ^15N, ^13C, and ^1H chemical shift assignments of the TIR domain of MyD88. The resonance assignments obtained in this work will contribute to the study of heteromeric TIR–TIR interactions between MyD88 and TIR-containing receptors or adaptors.
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structural basis for the multiple interactions of the myd88 tir domain in tlr4 signaling
Proceedings of the National Academy of Sciences of the United States of America, 2009Co-Authors: Hidenori Ohnishi, Hidehito Tochio, Zenichiro Kato, Takeshi Kimura, Hidekazu Hiroaki, Naomi Kondo, Kenji E Orii, Ailian Li, Masahiro ShirakawaAbstract:Myeloid Differentiating Factor 88 (MyD88) and MyD88 adaptor-like (Mal) are adaptor molecules critically involved in the Toll-like receptor (TLR) 4 signaling pathway. While Mal has been proposed to serve as a membrane-sorting adaptor, MyD88 mediates signal transduction from activated TLR4 to downstream components. The Toll/Interleukin-1 receptor (TIR) domain of MyD88 is responsible for sorting and signaling via direct or indirect TIR−TIR interactions between Mal and TLR4. However, the molecular mechanisms involved in multiple interactions of the TIR domain remain unclear. The present study describes the solution structure of the MyD88 TIR domain. Reporter gene assays revealed that 3 discrete surface sites in the TIR domain of MyD88 are important for TLR4 signaling. Two of these sites were shown to mediate direct binding to the TIR domain of Mal. Interestingly, Mal-TIR, but not MyD88-TIR, directly binds to the cytosolic TIR domain of TLR4. These observations suggested that the heteromeric assembly of TIR domains of the receptor and adaptors constitutes the initial step of TLR4 intracellular signal transduction.