The Experts below are selected from a list of 413040 Experts worldwide ranked by ideXlab platform
Rainer Stotzka - One of the best experts on this subject based on the ideXlab platform.
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an ensemble averaged cell density based Digital Model of zebrafish embryo development derived from light sheet microscopy data with single cell resolution
Scientific Reports, 2015Co-Authors: Andrei Yu Kobitski, Jens C Otte, Masanari Takamiya, Benjamin Schafer, Jonas Mertes, Johannes Stegmaier, Sepand Rastegar, F Rindone, Volker Hartmann, Rainer StotzkaAbstract:An ensemble-averaged, cell density-based Digital Model of zebrafish embryo development derived from light-sheet microscopy data with single-cell resolution
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an ensemble averaged cell density based Digital Model of zebrafish embryo development derived from light sheet microscopy data with single cell resolution
Scientific Reports, 2015Co-Authors: Andrei Yu Kobitski, Jens C Otte, Masanari Takamiya, Benjamin Schafer, Jonas Mertes, Johannes Stegmaier, Sepand Rastegar, F Rindone, Volker Hartmann, Rainer StotzkaAbstract:A new era in developmental biology has been ushered in by recent advances in the quantitative imaging of all-cell morphogenesis in living organisms. Here we have developed a light-sheet fluorescence microscopy-based framework with single-cell resolution for identification and characterization of subtle phenotypical changes of millimeter-sized organisms. Such a comparative study requires analyses of entire ensembles to be able to distinguish sample-to-sample variations from definitive phenotypical changes. We present a kinetic Digital Model of zebrafish embryos up to 16 h of development. The Model is based on the precise overlay and averaging of data taken on multiple individuals and describes the cell density and its migration direction at every point in time. Quantitative metrics for multi-sample comparative studies have been introduced to analyze developmental variations within the ensemble. The Digital Model may serve as a canvas on which the behavior of cellular subpopulations can be studied. As an example, we have investigated cellular rearrangements during germ layer formation at the onset of gastrulation. A comparison of the one-eyed pinhead (oep) mutant with the Digital Model of the wild-type embryo reveals its abnormal development at the onset of gastrulation, many hours before changes are obvious to the eye.
Andrei Yu Kobitski - One of the best experts on this subject based on the ideXlab platform.
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an ensemble averaged cell density based Digital Model of zebrafish embryo development derived from light sheet microscopy data with single cell resolution
Scientific Reports, 2015Co-Authors: Andrei Yu Kobitski, Jens C Otte, Masanari Takamiya, Benjamin Schafer, Jonas Mertes, Johannes Stegmaier, Sepand Rastegar, F Rindone, Volker Hartmann, Rainer StotzkaAbstract:An ensemble-averaged, cell density-based Digital Model of zebrafish embryo development derived from light-sheet microscopy data with single-cell resolution
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an ensemble averaged cell density based Digital Model of zebrafish embryo development derived from light sheet microscopy data with single cell resolution
Scientific Reports, 2015Co-Authors: Andrei Yu Kobitski, Jens C Otte, Masanari Takamiya, Benjamin Schafer, Jonas Mertes, Johannes Stegmaier, Sepand Rastegar, F Rindone, Volker Hartmann, Rainer StotzkaAbstract:A new era in developmental biology has been ushered in by recent advances in the quantitative imaging of all-cell morphogenesis in living organisms. Here we have developed a light-sheet fluorescence microscopy-based framework with single-cell resolution for identification and characterization of subtle phenotypical changes of millimeter-sized organisms. Such a comparative study requires analyses of entire ensembles to be able to distinguish sample-to-sample variations from definitive phenotypical changes. We present a kinetic Digital Model of zebrafish embryos up to 16 h of development. The Model is based on the precise overlay and averaging of data taken on multiple individuals and describes the cell density and its migration direction at every point in time. Quantitative metrics for multi-sample comparative studies have been introduced to analyze developmental variations within the ensemble. The Digital Model may serve as a canvas on which the behavior of cellular subpopulations can be studied. As an example, we have investigated cellular rearrangements during germ layer formation at the onset of gastrulation. A comparison of the one-eyed pinhead (oep) mutant with the Digital Model of the wild-type embryo reveals its abnormal development at the onset of gastrulation, many hours before changes are obvious to the eye.
Jens C Otte - One of the best experts on this subject based on the ideXlab platform.
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an ensemble averaged cell density based Digital Model of zebrafish embryo development derived from light sheet microscopy data with single cell resolution
Scientific Reports, 2015Co-Authors: Andrei Yu Kobitski, Jens C Otte, Masanari Takamiya, Benjamin Schafer, Jonas Mertes, Johannes Stegmaier, Sepand Rastegar, F Rindone, Volker Hartmann, Rainer StotzkaAbstract:An ensemble-averaged, cell density-based Digital Model of zebrafish embryo development derived from light-sheet microscopy data with single-cell resolution
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an ensemble averaged cell density based Digital Model of zebrafish embryo development derived from light sheet microscopy data with single cell resolution
Scientific Reports, 2015Co-Authors: Andrei Yu Kobitski, Jens C Otte, Masanari Takamiya, Benjamin Schafer, Jonas Mertes, Johannes Stegmaier, Sepand Rastegar, F Rindone, Volker Hartmann, Rainer StotzkaAbstract:A new era in developmental biology has been ushered in by recent advances in the quantitative imaging of all-cell morphogenesis in living organisms. Here we have developed a light-sheet fluorescence microscopy-based framework with single-cell resolution for identification and characterization of subtle phenotypical changes of millimeter-sized organisms. Such a comparative study requires analyses of entire ensembles to be able to distinguish sample-to-sample variations from definitive phenotypical changes. We present a kinetic Digital Model of zebrafish embryos up to 16 h of development. The Model is based on the precise overlay and averaging of data taken on multiple individuals and describes the cell density and its migration direction at every point in time. Quantitative metrics for multi-sample comparative studies have been introduced to analyze developmental variations within the ensemble. The Digital Model may serve as a canvas on which the behavior of cellular subpopulations can be studied. As an example, we have investigated cellular rearrangements during germ layer formation at the onset of gastrulation. A comparison of the one-eyed pinhead (oep) mutant with the Digital Model of the wild-type embryo reveals its abnormal development at the onset of gastrulation, many hours before changes are obvious to the eye.
Masanari Takamiya - One of the best experts on this subject based on the ideXlab platform.
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an ensemble averaged cell density based Digital Model of zebrafish embryo development derived from light sheet microscopy data with single cell resolution
Scientific Reports, 2015Co-Authors: Andrei Yu Kobitski, Jens C Otte, Masanari Takamiya, Benjamin Schafer, Jonas Mertes, Johannes Stegmaier, Sepand Rastegar, F Rindone, Volker Hartmann, Rainer StotzkaAbstract:An ensemble-averaged, cell density-based Digital Model of zebrafish embryo development derived from light-sheet microscopy data with single-cell resolution
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an ensemble averaged cell density based Digital Model of zebrafish embryo development derived from light sheet microscopy data with single cell resolution
Scientific Reports, 2015Co-Authors: Andrei Yu Kobitski, Jens C Otte, Masanari Takamiya, Benjamin Schafer, Jonas Mertes, Johannes Stegmaier, Sepand Rastegar, F Rindone, Volker Hartmann, Rainer StotzkaAbstract:A new era in developmental biology has been ushered in by recent advances in the quantitative imaging of all-cell morphogenesis in living organisms. Here we have developed a light-sheet fluorescence microscopy-based framework with single-cell resolution for identification and characterization of subtle phenotypical changes of millimeter-sized organisms. Such a comparative study requires analyses of entire ensembles to be able to distinguish sample-to-sample variations from definitive phenotypical changes. We present a kinetic Digital Model of zebrafish embryos up to 16 h of development. The Model is based on the precise overlay and averaging of data taken on multiple individuals and describes the cell density and its migration direction at every point in time. Quantitative metrics for multi-sample comparative studies have been introduced to analyze developmental variations within the ensemble. The Digital Model may serve as a canvas on which the behavior of cellular subpopulations can be studied. As an example, we have investigated cellular rearrangements during germ layer formation at the onset of gastrulation. A comparison of the one-eyed pinhead (oep) mutant with the Digital Model of the wild-type embryo reveals its abnormal development at the onset of gastrulation, many hours before changes are obvious to the eye.
Benjamin Schafer - One of the best experts on this subject based on the ideXlab platform.
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an ensemble averaged cell density based Digital Model of zebrafish embryo development derived from light sheet microscopy data with single cell resolution
Scientific Reports, 2015Co-Authors: Andrei Yu Kobitski, Jens C Otte, Masanari Takamiya, Benjamin Schafer, Jonas Mertes, Johannes Stegmaier, Sepand Rastegar, F Rindone, Volker Hartmann, Rainer StotzkaAbstract:An ensemble-averaged, cell density-based Digital Model of zebrafish embryo development derived from light-sheet microscopy data with single-cell resolution
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an ensemble averaged cell density based Digital Model of zebrafish embryo development derived from light sheet microscopy data with single cell resolution
Scientific Reports, 2015Co-Authors: Andrei Yu Kobitski, Jens C Otte, Masanari Takamiya, Benjamin Schafer, Jonas Mertes, Johannes Stegmaier, Sepand Rastegar, F Rindone, Volker Hartmann, Rainer StotzkaAbstract:A new era in developmental biology has been ushered in by recent advances in the quantitative imaging of all-cell morphogenesis in living organisms. Here we have developed a light-sheet fluorescence microscopy-based framework with single-cell resolution for identification and characterization of subtle phenotypical changes of millimeter-sized organisms. Such a comparative study requires analyses of entire ensembles to be able to distinguish sample-to-sample variations from definitive phenotypical changes. We present a kinetic Digital Model of zebrafish embryos up to 16 h of development. The Model is based on the precise overlay and averaging of data taken on multiple individuals and describes the cell density and its migration direction at every point in time. Quantitative metrics for multi-sample comparative studies have been introduced to analyze developmental variations within the ensemble. The Digital Model may serve as a canvas on which the behavior of cellular subpopulations can be studied. As an example, we have investigated cellular rearrangements during germ layer formation at the onset of gastrulation. A comparison of the one-eyed pinhead (oep) mutant with the Digital Model of the wild-type embryo reveals its abnormal development at the onset of gastrulation, many hours before changes are obvious to the eye.